Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia

Autores
Zhe, Hu; Kui, Guo; Cheng, Du; Jinhui, Sun; Naletoski, Ivancho; Xiaoyu, Chu; Yuezhi, Lin; Xuefeng, Wang; Barrandeguy, Maria Edith; Miño, Samuel; Wen, Wang; Lau, Patrick Imtung; Wernery, Ulrich; Raghavan, Rekha; Xiaojun, Wang
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is required to detect inapparent infection. In this study, a B-cell common epitope-based blocking ELISA (bELISA) was developed using a monoclonal antibody together with the EIAV p26 protein labelled with HRP. The test has been evaluated against the standard and with field serum samples globally. This bELISA test can be completed within 75 min, and the sensitivity is higher than those of either the AGID or one commercial cELISA kit. This bELISA assay was 8–16 times more analytically sensitive than AGID, and 2 to 4 times more analytically sensitive than one cELISA kit by testing three sera from the USA, Argentina, and China, respectively. The 353 serum samples from Argentina were tested, in comparison with AGID, the diagnostic sensitivity and specificity of our bELISA assay were 100% (154/154) and 97.0% (193/199), respectively, and the accuracy of the bELISA test was 98.3%. The bELISA test developed in this study is a rapid, sensitive, specific method for the detection of EIAV infection, and could be a promising candidate for use in the monitoring of the EIA epidemic worldwide.
Instituto de Virologia
Fil: Zhe, Hu. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Zhe, Hu. WOAH Reference Laboratory for Equine Infectious Anemia; China
Fil: Kui, Guo. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Cheng, Du. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Jinhui, Sun. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Jinhui, Sun. Northeast Agricultural University; China
Fil: Naletoski, Ivancho. International Atomic Energy Agency. Animal Production and Health Section. Joint FAO/IAEA Centre for Nuclear Applications in Food and Agriculture, Department of Nuclear Sciences and Applications; Austria
Fil: Xiaoyu, Chu. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Yuezhi, Lin. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Xuefeng, Wang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Barrandeguy, Maria Edith. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wen, Wang. Animal Health Supervision Institute of Xinjiang Uygur Autonomous Region; China
Fil: Lau, Patrick Imtung. Agriculture Fisheries and Conservation Department. Equine Disease Division; China
Fil: Wernery, Ulrich. Central Veterinary Research Laboratory; Emiratos Arabes Unidos
Fil: Raghavan, Rekha. Central Veterinary Research Laboratory; Emiratos Arabes Unidos
Fil: Xiaojun, Wang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Xiaojun, Wang. WOAH Reference Laboratory for Equine Infectious Anemia; China
Fuente
Applied Microbiology and Biotechnology 107 : 3305–3317. (2023)
Materia
Enfermedades de los Animales
Anemia Infecciosa Equina
Inmunodiagnóstico
Animal Diseases
Equine Infectious Anaemia
Immunodiagnosis
ELISA
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14713
Acceder
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oai_identifier_str oai:localhost:20.500.12123/14713
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemiaZhe, HuKui, GuoCheng, DuJinhui, SunNaletoski, IvanchoXiaoyu, ChuYuezhi, LinXuefeng, WangBarrandeguy, Maria EdithMiño, SamuelWen, WangLau, Patrick ImtungWernery, UlrichRaghavan, RekhaXiaojun, WangEnfermedades de los AnimalesAnemia Infecciosa EquinaInmunodiagnósticoAnimal DiseasesEquine Infectious AnaemiaImmunodiagnosisELISAEquine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is required to detect inapparent infection. In this study, a B-cell common epitope-based blocking ELISA (bELISA) was developed using a monoclonal antibody together with the EIAV p26 protein labelled with HRP. The test has been evaluated against the standard and with field serum samples globally. This bELISA test can be completed within 75 min, and the sensitivity is higher than those of either the AGID or one commercial cELISA kit. This bELISA assay was 8–16 times more analytically sensitive than AGID, and 2 to 4 times more analytically sensitive than one cELISA kit by testing three sera from the USA, Argentina, and China, respectively. The 353 serum samples from Argentina were tested, in comparison with AGID, the diagnostic sensitivity and specificity of our bELISA assay were 100% (154/154) and 97.0% (193/199), respectively, and the accuracy of the bELISA test was 98.3%. The bELISA test developed in this study is a rapid, sensitive, specific method for the detection of EIAV infection, and could be a promising candidate for use in the monitoring of the EIA epidemic worldwide.Instituto de VirologiaFil: Zhe, Hu. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Zhe, Hu. WOAH Reference Laboratory for Equine Infectious Anemia; ChinaFil: Kui, Guo. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Cheng, Du. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Jinhui, Sun. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Jinhui, Sun. Northeast Agricultural University; ChinaFil: Naletoski, Ivancho. International Atomic Energy Agency. Animal Production and Health Section. Joint FAO/IAEA Centre for Nuclear Applications in Food and Agriculture, Department of Nuclear Sciences and Applications; AustriaFil: Xiaoyu, Chu. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Yuezhi, Lin. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Xuefeng, Wang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Barrandeguy, Maria Edith. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Wen, Wang. Animal Health Supervision Institute of Xinjiang Uygur Autonomous Region; ChinaFil: Lau, Patrick Imtung. Agriculture Fisheries and Conservation Department. Equine Disease Division; ChinaFil: Wernery, Ulrich. Central Veterinary Research Laboratory; Emiratos Arabes UnidosFil: Raghavan, Rekha. Central Veterinary Research Laboratory; Emiratos Arabes UnidosFil: Xiaojun, Wang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Xiaojun, Wang. WOAH Reference Laboratory for Equine Infectious Anemia; ChinaSpringer2023-07-07T12:49:32Z2023-07-07T12:49:32Z2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14713https://link.springer.com/article/10.1007/s00253-023-12504-50175-75981432-0614https://doi.org/10.1007/s00253-023-12504-5Applied Microbiology and Biotechnology 107 : 3305–3317. (2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:24:38Zoai:localhost:20.500.12123/14713instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:24:38.537INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
title Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
spellingShingle Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
Zhe, Hu
Enfermedades de los Animales
Anemia Infecciosa Equina
Inmunodiagnóstico
Animal Diseases
Equine Infectious Anaemia
Immunodiagnosis
ELISA
title_short Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
title_full Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
title_fullStr Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
title_full_unstemmed Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
title_sort Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
dc.creator.none.fl_str_mv Zhe, Hu
Kui, Guo
Cheng, Du
Jinhui, Sun
Naletoski, Ivancho
Xiaoyu, Chu
Yuezhi, Lin
Xuefeng, Wang
Barrandeguy, Maria Edith
Miño, Samuel
Wen, Wang
Lau, Patrick Imtung
Wernery, Ulrich
Raghavan, Rekha
Xiaojun, Wang
author Zhe, Hu
author_facet Zhe, Hu
Kui, Guo
Cheng, Du
Jinhui, Sun
Naletoski, Ivancho
Xiaoyu, Chu
Yuezhi, Lin
Xuefeng, Wang
Barrandeguy, Maria Edith
Miño, Samuel
Wen, Wang
Lau, Patrick Imtung
Wernery, Ulrich
Raghavan, Rekha
Xiaojun, Wang
author_role author
author2 Kui, Guo
Cheng, Du
Jinhui, Sun
Naletoski, Ivancho
Xiaoyu, Chu
Yuezhi, Lin
Xuefeng, Wang
Barrandeguy, Maria Edith
Miño, Samuel
Wen, Wang
Lau, Patrick Imtung
Wernery, Ulrich
Raghavan, Rekha
Xiaojun, Wang
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Anemia Infecciosa Equina
Inmunodiagnóstico
Animal Diseases
Equine Infectious Anaemia
Immunodiagnosis
ELISA
topic Enfermedades de los Animales
Anemia Infecciosa Equina
Inmunodiagnóstico
Animal Diseases
Equine Infectious Anaemia
Immunodiagnosis
ELISA
dc.description.none.fl_txt_mv Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is required to detect inapparent infection. In this study, a B-cell common epitope-based blocking ELISA (bELISA) was developed using a monoclonal antibody together with the EIAV p26 protein labelled with HRP. The test has been evaluated against the standard and with field serum samples globally. This bELISA test can be completed within 75 min, and the sensitivity is higher than those of either the AGID or one commercial cELISA kit. This bELISA assay was 8–16 times more analytically sensitive than AGID, and 2 to 4 times more analytically sensitive than one cELISA kit by testing three sera from the USA, Argentina, and China, respectively. The 353 serum samples from Argentina were tested, in comparison with AGID, the diagnostic sensitivity and specificity of our bELISA assay were 100% (154/154) and 97.0% (193/199), respectively, and the accuracy of the bELISA test was 98.3%. The bELISA test developed in this study is a rapid, sensitive, specific method for the detection of EIAV infection, and could be a promising candidate for use in the monitoring of the EIA epidemic worldwide.
Instituto de Virologia
Fil: Zhe, Hu. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Zhe, Hu. WOAH Reference Laboratory for Equine Infectious Anemia; China
Fil: Kui, Guo. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Cheng, Du. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Jinhui, Sun. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Jinhui, Sun. Northeast Agricultural University; China
Fil: Naletoski, Ivancho. International Atomic Energy Agency. Animal Production and Health Section. Joint FAO/IAEA Centre for Nuclear Applications in Food and Agriculture, Department of Nuclear Sciences and Applications; Austria
Fil: Xiaoyu, Chu. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Yuezhi, Lin. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Xuefeng, Wang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Barrandeguy, Maria Edith. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wen, Wang. Animal Health Supervision Institute of Xinjiang Uygur Autonomous Region; China
Fil: Lau, Patrick Imtung. Agriculture Fisheries and Conservation Department. Equine Disease Division; China
Fil: Wernery, Ulrich. Central Veterinary Research Laboratory; Emiratos Arabes Unidos
Fil: Raghavan, Rekha. Central Veterinary Research Laboratory; Emiratos Arabes Unidos
Fil: Xiaojun, Wang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Xiaojun, Wang. WOAH Reference Laboratory for Equine Infectious Anemia; China
description Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is required to detect inapparent infection. In this study, a B-cell common epitope-based blocking ELISA (bELISA) was developed using a monoclonal antibody together with the EIAV p26 protein labelled with HRP. The test has been evaluated against the standard and with field serum samples globally. This bELISA test can be completed within 75 min, and the sensitivity is higher than those of either the AGID or one commercial cELISA kit. This bELISA assay was 8–16 times more analytically sensitive than AGID, and 2 to 4 times more analytically sensitive than one cELISA kit by testing three sera from the USA, Argentina, and China, respectively. The 353 serum samples from Argentina were tested, in comparison with AGID, the diagnostic sensitivity and specificity of our bELISA assay were 100% (154/154) and 97.0% (193/199), respectively, and the accuracy of the bELISA test was 98.3%. The bELISA test developed in this study is a rapid, sensitive, specific method for the detection of EIAV infection, and could be a promising candidate for use in the monitoring of the EIA epidemic worldwide.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-07T12:49:32Z
2023-07-07T12:49:32Z
2023-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14713
https://link.springer.com/article/10.1007/s00253-023-12504-5
0175-7598
1432-0614
https://doi.org/10.1007/s00253-023-12504-5
url http://hdl.handle.net/20.500.12123/14713
https://link.springer.com/article/10.1007/s00253-023-12504-5
https://doi.org/10.1007/s00253-023-12504-5
identifier_str_mv 0175-7598
1432-0614
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Applied Microbiology and Biotechnology 107 : 3305–3317. (2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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