Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses

Autores
Costa, Sofia; Sastre, Patricia; Pérez, Teresa; Tapia, Istar; Barrandeguy, Maria Edith; Sanchez-Vizcaino, José Manuel; Sanchez-Matamoros, Almudena; Wigdorovitz, Andres; Sanz, Antonio; Rueda, Paloma
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
African horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products over the past decades, together with the socio-economic impact of previous AHS and EIA outbreaks, there is a clear demand for an early discrimination and a strict control of their transmission between enzootic and AHS/EIA-free regions. Currently, the individual control and prevention of AHS or EIA relies on a series of measures, including the restriction of animal movements, vector control, and the use of several laboratory techniques for viral identification, amongst others. Despite being widely employed in surveillance programmes and in the control of animal movements, the available serological assays can only detect AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow assay (LFA) for simultaneous detection and differentiation of specific antibodies against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 outer core protein and the EIAV-P26 major core protein. The results indicated that the duplex LFA presented a good analytical performance, detecting simultaneously and specifically antibodies against AHSV and EIAV. The initial diagnostic evaluation revealed a good agreement with results from the AHS and EIA tests prescribed by the OIE, and it highlighted the usefulness of the new AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis.
Inst.de Virología
Fil: Costa, Sofia. Inmunología y Genética Aplicada S.A.; España
Fil: Sastre, Patricia. Inmunología y Genética Aplicada S.A.; España
Fil: Pérez, Teresa. Inmunología y Genética Aplicada S.A.; España
Fil: Tapia, Istar. Inmunología y Genética Aplicada S.A.; España
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Fil: Sanchez-Vizcaino, José Manuel. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Sanidad Animal; España. VISAVET Health Surveillance Centre; España
Fil: Sanchez-Matamoros, Almudena. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Sanidad Animal; España. VISAVET Health Surveillance Centre; España
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rueda, Paloma. Inmunología y Genética Aplicada S.A.; España
Fuente
Journal of virological methods 237 : 127-131. (November 2016)
Materia
Enfermedades de los Animales
Anemia Infecciosa Equina
Peste Equina Africana
Anticuerpos
Animal Diseases
Equine Infectious Anaemia
African Horse Sickness
Antibodies
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1369
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1369
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spelling Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia virusesCosta, SofiaSastre, PatriciaPérez, TeresaTapia, IstarBarrandeguy, Maria EdithSanchez-Vizcaino, José ManuelSanchez-Matamoros, AlmudenaWigdorovitz, AndresSanz, AntonioRueda, PalomaEnfermedades de los AnimalesAnemia Infecciosa EquinaPeste Equina AfricanaAnticuerposAnimal DiseasesEquine Infectious AnaemiaAfrican Horse SicknessAntibodiesAfrican horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products over the past decades, together with the socio-economic impact of previous AHS and EIA outbreaks, there is a clear demand for an early discrimination and a strict control of their transmission between enzootic and AHS/EIA-free regions. Currently, the individual control and prevention of AHS or EIA relies on a series of measures, including the restriction of animal movements, vector control, and the use of several laboratory techniques for viral identification, amongst others. Despite being widely employed in surveillance programmes and in the control of animal movements, the available serological assays can only detect AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow assay (LFA) for simultaneous detection and differentiation of specific antibodies against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 outer core protein and the EIAV-P26 major core protein. The results indicated that the duplex LFA presented a good analytical performance, detecting simultaneously and specifically antibodies against AHSV and EIAV. The initial diagnostic evaluation revealed a good agreement with results from the AHS and EIA tests prescribed by the OIE, and it highlighted the usefulness of the new AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis.Inst.de VirologíaFil: Costa, Sofia. Inmunología y Genética Aplicada S.A.; EspañaFil: Sastre, Patricia. Inmunología y Genética Aplicada S.A.; EspañaFil: Pérez, Teresa. Inmunología y Genética Aplicada S.A.; EspañaFil: Tapia, Istar. Inmunología y Genética Aplicada S.A.; EspañaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; ArgentinaFil: Sanchez-Vizcaino, José Manuel. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Sanidad Animal; España. VISAVET Health Surveillance Centre; EspañaFil: Sanchez-Matamoros, Almudena. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Sanidad Animal; España. VISAVET Health Surveillance Centre; EspañaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rueda, Paloma. Inmunología y Genética Aplicada S.A.; España2017-10-02T11:48:21Z2017-10-02T11:48:21Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1369http://www.sciencedirect.com/science/article/pii/S0166093416301641?via%3Dihub0166-0934 (Print)1879-0984 (Online)https://doi.org/10.1016/j.jviromet.2016.09.009Journal of virological methods 237 : 127-131. (November 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:01Zoai:localhost:20.500.12123/1369instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:02.051INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
title Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
spellingShingle Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
Costa, Sofia
Enfermedades de los Animales
Anemia Infecciosa Equina
Peste Equina Africana
Anticuerpos
Animal Diseases
Equine Infectious Anaemia
African Horse Sickness
Antibodies
title_short Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
title_full Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
title_fullStr Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
title_full_unstemmed Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
title_sort Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
dc.creator.none.fl_str_mv Costa, Sofia
Sastre, Patricia
Pérez, Teresa
Tapia, Istar
Barrandeguy, Maria Edith
Sanchez-Vizcaino, José Manuel
Sanchez-Matamoros, Almudena
Wigdorovitz, Andres
Sanz, Antonio
Rueda, Paloma
author Costa, Sofia
author_facet Costa, Sofia
Sastre, Patricia
Pérez, Teresa
Tapia, Istar
Barrandeguy, Maria Edith
Sanchez-Vizcaino, José Manuel
Sanchez-Matamoros, Almudena
Wigdorovitz, Andres
Sanz, Antonio
Rueda, Paloma
author_role author
author2 Sastre, Patricia
Pérez, Teresa
Tapia, Istar
Barrandeguy, Maria Edith
Sanchez-Vizcaino, José Manuel
Sanchez-Matamoros, Almudena
Wigdorovitz, Andres
Sanz, Antonio
Rueda, Paloma
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Anemia Infecciosa Equina
Peste Equina Africana
Anticuerpos
Animal Diseases
Equine Infectious Anaemia
African Horse Sickness
Antibodies
topic Enfermedades de los Animales
Anemia Infecciosa Equina
Peste Equina Africana
Anticuerpos
Animal Diseases
Equine Infectious Anaemia
African Horse Sickness
Antibodies
dc.description.none.fl_txt_mv African horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products over the past decades, together with the socio-economic impact of previous AHS and EIA outbreaks, there is a clear demand for an early discrimination and a strict control of their transmission between enzootic and AHS/EIA-free regions. Currently, the individual control and prevention of AHS or EIA relies on a series of measures, including the restriction of animal movements, vector control, and the use of several laboratory techniques for viral identification, amongst others. Despite being widely employed in surveillance programmes and in the control of animal movements, the available serological assays can only detect AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow assay (LFA) for simultaneous detection and differentiation of specific antibodies against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 outer core protein and the EIAV-P26 major core protein. The results indicated that the duplex LFA presented a good analytical performance, detecting simultaneously and specifically antibodies against AHSV and EIAV. The initial diagnostic evaluation revealed a good agreement with results from the AHS and EIA tests prescribed by the OIE, and it highlighted the usefulness of the new AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis.
Inst.de Virología
Fil: Costa, Sofia. Inmunología y Genética Aplicada S.A.; España
Fil: Sastre, Patricia. Inmunología y Genética Aplicada S.A.; España
Fil: Pérez, Teresa. Inmunología y Genética Aplicada S.A.; España
Fil: Tapia, Istar. Inmunología y Genética Aplicada S.A.; España
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Fil: Sanchez-Vizcaino, José Manuel. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Sanidad Animal; España. VISAVET Health Surveillance Centre; España
Fil: Sanchez-Matamoros, Almudena. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Sanidad Animal; España. VISAVET Health Surveillance Centre; España
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rueda, Paloma. Inmunología y Genética Aplicada S.A.; España
description African horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products over the past decades, together with the socio-economic impact of previous AHS and EIA outbreaks, there is a clear demand for an early discrimination and a strict control of their transmission between enzootic and AHS/EIA-free regions. Currently, the individual control and prevention of AHS or EIA relies on a series of measures, including the restriction of animal movements, vector control, and the use of several laboratory techniques for viral identification, amongst others. Despite being widely employed in surveillance programmes and in the control of animal movements, the available serological assays can only detect AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow assay (LFA) for simultaneous detection and differentiation of specific antibodies against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 outer core protein and the EIAV-P26 major core protein. The results indicated that the duplex LFA presented a good analytical performance, detecting simultaneously and specifically antibodies against AHSV and EIAV. The initial diagnostic evaluation revealed a good agreement with results from the AHS and EIA tests prescribed by the OIE, and it highlighted the usefulness of the new AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-10-02T11:48:21Z
2017-10-02T11:48:21Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1369
http://www.sciencedirect.com/science/article/pii/S0166093416301641?via%3Dihub
0166-0934 (Print)
1879-0984 (Online)
https://doi.org/10.1016/j.jviromet.2016.09.009
url http://hdl.handle.net/20.500.12123/1369
http://www.sciencedirect.com/science/article/pii/S0166093416301641?via%3Dihub
https://doi.org/10.1016/j.jviromet.2016.09.009
identifier_str_mv 0166-0934 (Print)
1879-0984 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of virological methods 237 : 127-131. (November 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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