P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum

Autores
Mira, Anabela; Garro, Carlos Javier; De Alba, Paloma; Monti, Demián Esteban; Lang, Maria Cecilia; Vivas, Alejandro; Medina, Esteban; Franco, Juan Cruz; Gutierrez, Álvaro; Schnittger, Leonhard; Wigdorovitz, Andres; Parreño, Gladys Viviana; Bok, Marina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background/Objectives: Cryptosporidium parvum is a zoonotic enteroparasite causing severe diarrhea in newborn calves, leading to significant economic losses in dairy and beef farming. This study aimed to evaluate whether C. parvum p23-specific IgY antibodies could control neonatal calf diarrhea caused by C. parvum. Methods: A recombinant immunogen comprising the p23 protein fused to the antigen-presenting cell homing (APCH) molecule was expressed using the baculovirus system. Hens were immunized with the APCH-p23 immunogen, and the resulting IgY was spray-dried for treatment use. Eight newborn calves were included in the study and received commercial colostrum within the first 12 h of life. Four calves were treated with 20 g of powdered egg containing IgY (p23-specific IgY titer of 256 in milk) twice daily for 7 days. The remaining four calves received regular non-supplemented milk. All calves were orally infected with 6 million oocysts and monitored for 21 days. Results: Calves treated with p23-specific IgY exhibited significantly reduced diarrhea duration (3.5 vs. 7.5 days; p = 0.0397) and oocyst shedding duration (6.50 vs. 12 days; p = 0.0089). In addition, the total number of excreted oocysts, as measured by the change of the area under the curve (AUC), was significantly reduced in the treated group (14.25 vs. 33.45; p = 0.0117). Although the onset of diarrhea was delayed (3.5 to 6.5 days post-infection; p = 0.1840), and diarrhea severity was reduced (24.25 to 17 AUC; p = 0.1236), both parameters were not statistically significant. Conclusions: P23-specific IgY antibodies effectively reduced the C. parvum-induced duration of diarrhea in experimentally infected calves. These findings highlight the potential of this passive treatment as a promising strategy for controlling neonatal calf diarrhea.
Instituto de Patobiología
Fil: Mira, Anabela. Bioinnovo SA; Argentina
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Monti, Demian Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Lang, Maria Cecilia. Bioinnovo SA; Argentina
Fil: Vivas, Alejandro. Bioinnovo SA; Argentina
Fil: Medina, Esteban. Bioinnovo SA; Argentina
Fil: Franco, Juan Cruz. Bioinnovo SA; Argentina
Fil: Gutierrez, Álvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas; Argentina
Fuente
Vaccines 13 (2) : 162 (February 2025)
Materia
Cryptosporidium parvum
Calves
Diarrhoea
Oocysts
Ternero
Diarrea
Oocisto
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21518
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21518
network_acronym_str INTADig
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spelling P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvumMira, AnabelaGarro, Carlos JavierDe Alba, PalomaMonti, Demián EstebanLang, Maria CeciliaVivas, AlejandroMedina, EstebanFranco, Juan CruzGutierrez, ÁlvaroSchnittger, LeonhardWigdorovitz, AndresParreño, Gladys VivianaBok, MarinaCryptosporidium parvumCalvesDiarrhoeaOocystsTerneroDiarreaOocistoBackground/Objectives: Cryptosporidium parvum is a zoonotic enteroparasite causing severe diarrhea in newborn calves, leading to significant economic losses in dairy and beef farming. This study aimed to evaluate whether C. parvum p23-specific IgY antibodies could control neonatal calf diarrhea caused by C. parvum. Methods: A recombinant immunogen comprising the p23 protein fused to the antigen-presenting cell homing (APCH) molecule was expressed using the baculovirus system. Hens were immunized with the APCH-p23 immunogen, and the resulting IgY was spray-dried for treatment use. Eight newborn calves were included in the study and received commercial colostrum within the first 12 h of life. Four calves were treated with 20 g of powdered egg containing IgY (p23-specific IgY titer of 256 in milk) twice daily for 7 days. The remaining four calves received regular non-supplemented milk. All calves were orally infected with 6 million oocysts and monitored for 21 days. Results: Calves treated with p23-specific IgY exhibited significantly reduced diarrhea duration (3.5 vs. 7.5 days; p = 0.0397) and oocyst shedding duration (6.50 vs. 12 days; p = 0.0089). In addition, the total number of excreted oocysts, as measured by the change of the area under the curve (AUC), was significantly reduced in the treated group (14.25 vs. 33.45; p = 0.0117). Although the onset of diarrhea was delayed (3.5 to 6.5 days post-infection; p = 0.1840), and diarrhea severity was reduced (24.25 to 17 AUC; p = 0.1236), both parameters were not statistically significant. Conclusions: P23-specific IgY antibodies effectively reduced the C. parvum-induced duration of diarrhea in experimentally infected calves. These findings highlight the potential of this passive treatment as a promising strategy for controlling neonatal calf diarrhea.Instituto de PatobiologíaFil: Mira, Anabela. Bioinnovo SA; ArgentinaFil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Monti, Demian Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); ArgentinaFil: Lang, Maria Cecilia. Bioinnovo SA; ArgentinaFil: Vivas, Alejandro. Bioinnovo SA; ArgentinaFil: Medina, Esteban. Bioinnovo SA; ArgentinaFil: Franco, Juan Cruz. Bioinnovo SA; ArgentinaFil: Gutierrez, Álvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); ArgentinaFil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); ArgentinaFil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas; ArgentinaMDPI2025-02-28T13:55:53Z2025-02-28T13:55:53Z2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21518https://www.mdpi.com/2076-393X/13/2/1622076-393Xhttps://doi.org/10.3390/vaccines13020162Vaccines 13 (2) : 162 (February 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Saludinfo:eu-repograntAgreement/INTA/2023-PD-L01-I118, Innovaciones en los procesos de crianza, recría y vaca en transición de rodeos lecherosinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:25:37Zoai:localhost:20.500.12123/21518instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:25:38.201INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
title P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
spellingShingle P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
Mira, Anabela
Cryptosporidium parvum
Calves
Diarrhoea
Oocysts
Ternero
Diarrea
Oocisto
title_short P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
title_full P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
title_fullStr P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
title_full_unstemmed P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
title_sort P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
dc.creator.none.fl_str_mv Mira, Anabela
Garro, Carlos Javier
De Alba, Paloma
Monti, Demián Esteban
Lang, Maria Cecilia
Vivas, Alejandro
Medina, Esteban
Franco, Juan Cruz
Gutierrez, Álvaro
Schnittger, Leonhard
Wigdorovitz, Andres
Parreño, Gladys Viviana
Bok, Marina
author Mira, Anabela
author_facet Mira, Anabela
Garro, Carlos Javier
De Alba, Paloma
Monti, Demián Esteban
Lang, Maria Cecilia
Vivas, Alejandro
Medina, Esteban
Franco, Juan Cruz
Gutierrez, Álvaro
Schnittger, Leonhard
Wigdorovitz, Andres
Parreño, Gladys Viviana
Bok, Marina
author_role author
author2 Garro, Carlos Javier
De Alba, Paloma
Monti, Demián Esteban
Lang, Maria Cecilia
Vivas, Alejandro
Medina, Esteban
Franco, Juan Cruz
Gutierrez, Álvaro
Schnittger, Leonhard
Wigdorovitz, Andres
Parreño, Gladys Viviana
Bok, Marina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cryptosporidium parvum
Calves
Diarrhoea
Oocysts
Ternero
Diarrea
Oocisto
topic Cryptosporidium parvum
Calves
Diarrhoea
Oocysts
Ternero
Diarrea
Oocisto
dc.description.none.fl_txt_mv Background/Objectives: Cryptosporidium parvum is a zoonotic enteroparasite causing severe diarrhea in newborn calves, leading to significant economic losses in dairy and beef farming. This study aimed to evaluate whether C. parvum p23-specific IgY antibodies could control neonatal calf diarrhea caused by C. parvum. Methods: A recombinant immunogen comprising the p23 protein fused to the antigen-presenting cell homing (APCH) molecule was expressed using the baculovirus system. Hens were immunized with the APCH-p23 immunogen, and the resulting IgY was spray-dried for treatment use. Eight newborn calves were included in the study and received commercial colostrum within the first 12 h of life. Four calves were treated with 20 g of powdered egg containing IgY (p23-specific IgY titer of 256 in milk) twice daily for 7 days. The remaining four calves received regular non-supplemented milk. All calves were orally infected with 6 million oocysts and monitored for 21 days. Results: Calves treated with p23-specific IgY exhibited significantly reduced diarrhea duration (3.5 vs. 7.5 days; p = 0.0397) and oocyst shedding duration (6.50 vs. 12 days; p = 0.0089). In addition, the total number of excreted oocysts, as measured by the change of the area under the curve (AUC), was significantly reduced in the treated group (14.25 vs. 33.45; p = 0.0117). Although the onset of diarrhea was delayed (3.5 to 6.5 days post-infection; p = 0.1840), and diarrhea severity was reduced (24.25 to 17 AUC; p = 0.1236), both parameters were not statistically significant. Conclusions: P23-specific IgY antibodies effectively reduced the C. parvum-induced duration of diarrhea in experimentally infected calves. These findings highlight the potential of this passive treatment as a promising strategy for controlling neonatal calf diarrhea.
Instituto de Patobiología
Fil: Mira, Anabela. Bioinnovo SA; Argentina
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Monti, Demian Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Lang, Maria Cecilia. Bioinnovo SA; Argentina
Fil: Vivas, Alejandro. Bioinnovo SA; Argentina
Fil: Medina, Esteban. Bioinnovo SA; Argentina
Fil: Franco, Juan Cruz. Bioinnovo SA; Argentina
Fil: Gutierrez, Álvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas; Argentina
description Background/Objectives: Cryptosporidium parvum is a zoonotic enteroparasite causing severe diarrhea in newborn calves, leading to significant economic losses in dairy and beef farming. This study aimed to evaluate whether C. parvum p23-specific IgY antibodies could control neonatal calf diarrhea caused by C. parvum. Methods: A recombinant immunogen comprising the p23 protein fused to the antigen-presenting cell homing (APCH) molecule was expressed using the baculovirus system. Hens were immunized with the APCH-p23 immunogen, and the resulting IgY was spray-dried for treatment use. Eight newborn calves were included in the study and received commercial colostrum within the first 12 h of life. Four calves were treated with 20 g of powdered egg containing IgY (p23-specific IgY titer of 256 in milk) twice daily for 7 days. The remaining four calves received regular non-supplemented milk. All calves were orally infected with 6 million oocysts and monitored for 21 days. Results: Calves treated with p23-specific IgY exhibited significantly reduced diarrhea duration (3.5 vs. 7.5 days; p = 0.0397) and oocyst shedding duration (6.50 vs. 12 days; p = 0.0089). In addition, the total number of excreted oocysts, as measured by the change of the area under the curve (AUC), was significantly reduced in the treated group (14.25 vs. 33.45; p = 0.0117). Although the onset of diarrhea was delayed (3.5 to 6.5 days post-infection; p = 0.1840), and diarrhea severity was reduced (24.25 to 17 AUC; p = 0.1236), both parameters were not statistically significant. Conclusions: P23-specific IgY antibodies effectively reduced the C. parvum-induced duration of diarrhea in experimentally infected calves. These findings highlight the potential of this passive treatment as a promising strategy for controlling neonatal calf diarrhea.
publishDate 2025
dc.date.none.fl_str_mv 2025-02-28T13:55:53Z
2025-02-28T13:55:53Z
2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21518
https://www.mdpi.com/2076-393X/13/2/162
2076-393X
https://doi.org/10.3390/vaccines13020162
url http://hdl.handle.net/20.500.12123/21518
https://www.mdpi.com/2076-393X/13/2/162
https://doi.org/10.3390/vaccines13020162
identifier_str_mv 2076-393X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud
info:eu-repograntAgreement/INTA/2023-PD-L01-I118, Innovaciones en los procesos de crianza, recría y vaca en transición de rodeos lecheros
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Vaccines 13 (2) : 162 (February 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
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repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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