Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development

Autores
Coluccio Leskow, Carla; Dominguez, Pia Guadalupe; Díaz Zirpolo, José Antonio; Obata, Toshihiro; Kamenetzky, Laura; Costa, Hernán; Taboga, Oscar Alberto; Marti, Hector Ruben; Keurentjes, Joost; Sulpice, Ronan; Ishihara, Hirofumi; Stitt, Mark; Fernie, Alisdair R.; Carrari, Fernando
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.
Inst. de Biotecnología
Fil: Coluccio Leskow, Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Díaz Zirpolo, José Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; Holanda
Fil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Journal of experimental botany 67 (14) : 4091–4103 (July 2016)
Materia
Fitomejoramiento
Arabidopsis
Biomasa
Loci de Rasgos Cuantitativos
Plant Breeding
Biomass
Quantitative Trait Loci
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1187
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1187
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant developmentColuccio Leskow, CarlaDominguez, Pia GuadalupeDíaz Zirpolo, José AntonioObata, ToshihiroKamenetzky, LauraCosta, HernánTaboga, Oscar AlbertoMarti, Hector RubenKeurentjes, JoostSulpice, RonanIshihara, HirofumiStitt, MarkFernie, Alisdair R.Carrari, FernandoFitomejoramientoArabidopsisBiomasaLoci de Rasgos CuantitativosPlant BreedingBiomassQuantitative Trait LociImproving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.Inst. de BiotecnologíaFil: Coluccio Leskow, Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Díaz Zirpolo, José Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; HolandaFil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina2017-09-11T12:55:58Z2017-09-11T12:55:58Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1187https://academic.oup.com/jxb/article/67/14/4091/21976440022-0957 (Print)1460-2431 (Online)https://doi.org/10.1093/jxb/erw185Journal of experimental botany 67 (14) : 4091–4103 (July 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:10Zoai:localhost:20.500.12123/1187instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:10.822INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
spellingShingle Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
Coluccio Leskow, Carla
Fitomejoramiento
Arabidopsis
Biomasa
Loci de Rasgos Cuantitativos
Plant Breeding
Biomass
Quantitative Trait Loci
title_short Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title_full Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title_fullStr Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title_full_unstemmed Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title_sort Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
dc.creator.none.fl_str_mv Coluccio Leskow, Carla
Dominguez, Pia Guadalupe
Díaz Zirpolo, José Antonio
Obata, Toshihiro
Kamenetzky, Laura
Costa, Hernán
Taboga, Oscar Alberto
Marti, Hector Ruben
Keurentjes, Joost
Sulpice, Ronan
Ishihara, Hirofumi
Stitt, Mark
Fernie, Alisdair R.
Carrari, Fernando
author Coluccio Leskow, Carla
author_facet Coluccio Leskow, Carla
Dominguez, Pia Guadalupe
Díaz Zirpolo, José Antonio
Obata, Toshihiro
Kamenetzky, Laura
Costa, Hernán
Taboga, Oscar Alberto
Marti, Hector Ruben
Keurentjes, Joost
Sulpice, Ronan
Ishihara, Hirofumi
Stitt, Mark
Fernie, Alisdair R.
Carrari, Fernando
author_role author
author2 Dominguez, Pia Guadalupe
Díaz Zirpolo, José Antonio
Obata, Toshihiro
Kamenetzky, Laura
Costa, Hernán
Taboga, Oscar Alberto
Marti, Hector Ruben
Keurentjes, Joost
Sulpice, Ronan
Ishihara, Hirofumi
Stitt, Mark
Fernie, Alisdair R.
Carrari, Fernando
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fitomejoramiento
Arabidopsis
Biomasa
Loci de Rasgos Cuantitativos
Plant Breeding
Biomass
Quantitative Trait Loci
topic Fitomejoramiento
Arabidopsis
Biomasa
Loci de Rasgos Cuantitativos
Plant Breeding
Biomass
Quantitative Trait Loci
dc.description.none.fl_txt_mv Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.
Inst. de Biotecnología
Fil: Coluccio Leskow, Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Díaz Zirpolo, José Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; Holanda
Fil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-09-11T12:55:58Z
2017-09-11T12:55:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1187
https://academic.oup.com/jxb/article/67/14/4091/2197644
0022-0957 (Print)
1460-2431 (Online)
https://doi.org/10.1093/jxb/erw185
url http://hdl.handle.net/20.500.12123/1187
https://academic.oup.com/jxb/article/67/14/4091/2197644
https://doi.org/10.1093/jxb/erw185
identifier_str_mv 0022-0957 (Print)
1460-2431 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of experimental botany 67 (14) : 4091–4103 (July 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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