Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development

Autores
Coluccio Leskow, Carla; Kamenetzky, Laura; Dominguez, Pia Guadalupe; Diaz Zirpolo, Jose Antonio; Obata, Toshihiro; Costa, Hernán; Marti, Marcelo Adrian; Taboga, Oscar Alberto; Keurentjes, Joost; Sulpice, Ronan; Ishihara, Hirofumi; Stitt, Mark; Fernie, Alisdair Robert; Carrari, Fernando Oscar
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar Km for both alleles and a slightly higher Vmax for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vacInv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.
Fil: Coluccio Leskow, Carla. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diaz Zirpolo, Jose Antonio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Costa, Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; Países Bajos
Fil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Genomica
Metabolomica
Arabidopsis
Biomass
Inhibitor
Near Isogenic Line
Quantitative Trait Loci
Vacuolar Invertase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46969
Acceder
id CONICETDig_afb6a93e34619f39fe24f8be50d4e258
oai_identifier_str oai:ri.conicet.gov.ar:11336/46969
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant developmentColuccio Leskow, CarlaKamenetzky, LauraDominguez, Pia GuadalupeDiaz Zirpolo, Jose AntonioObata, ToshihiroCosta, HernánMarti, Marcelo AdrianTaboga, Oscar AlbertoKeurentjes, JoostSulpice, RonanIshihara, HirofumiStitt, MarkFernie, Alisdair RobertCarrari, Fernando OscarGenomicaMetabolomicaArabidopsisBiomassInhibitorNear Isogenic LineQuantitative Trait LociVacuolar Invertasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar Km for both alleles and a slightly higher Vmax for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vacInv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.Fil: Coluccio Leskow, Carla. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diaz Zirpolo, Jose Antonio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Costa, Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; ArgentinaFil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; Países BajosFil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaOxford University Press2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46969Coluccio Leskow, Carla; Kamenetzky, Laura; Dominguez, Pia Guadalupe; Diaz Zirpolo, Jose Antonio; Obata, Toshihiro; et al.; Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development; Oxford University Press; Journal of Experimental Botany; 67; 14; 7-2016; 4091-41030022-09571460-2431CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/67/14/4091/2197644info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erw185info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:36Zoai:ri.conicet.gov.ar:11336/46969instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:36.832CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
spellingShingle Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
Coluccio Leskow, Carla
Genomica
Metabolomica
Arabidopsis
Biomass
Inhibitor
Near Isogenic Line
Quantitative Trait Loci
Vacuolar Invertase
title_short Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title_full Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title_fullStr Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title_full_unstemmed Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
title_sort Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
dc.creator.none.fl_str_mv Coluccio Leskow, Carla
Kamenetzky, Laura
Dominguez, Pia Guadalupe
Diaz Zirpolo, Jose Antonio
Obata, Toshihiro
Costa, Hernán
Marti, Marcelo Adrian
Taboga, Oscar Alberto
Keurentjes, Joost
Sulpice, Ronan
Ishihara, Hirofumi
Stitt, Mark
Fernie, Alisdair Robert
Carrari, Fernando Oscar
author Coluccio Leskow, Carla
author_facet Coluccio Leskow, Carla
Kamenetzky, Laura
Dominguez, Pia Guadalupe
Diaz Zirpolo, Jose Antonio
Obata, Toshihiro
Costa, Hernán
Marti, Marcelo Adrian
Taboga, Oscar Alberto
Keurentjes, Joost
Sulpice, Ronan
Ishihara, Hirofumi
Stitt, Mark
Fernie, Alisdair Robert
Carrari, Fernando Oscar
author_role author
author2 Kamenetzky, Laura
Dominguez, Pia Guadalupe
Diaz Zirpolo, Jose Antonio
Obata, Toshihiro
Costa, Hernán
Marti, Marcelo Adrian
Taboga, Oscar Alberto
Keurentjes, Joost
Sulpice, Ronan
Ishihara, Hirofumi
Stitt, Mark
Fernie, Alisdair Robert
Carrari, Fernando Oscar
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genomica
Metabolomica
Arabidopsis
Biomass
Inhibitor
Near Isogenic Line
Quantitative Trait Loci
Vacuolar Invertase
topic Genomica
Metabolomica
Arabidopsis
Biomass
Inhibitor
Near Isogenic Line
Quantitative Trait Loci
Vacuolar Invertase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar Km for both alleles and a slightly higher Vmax for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vacInv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.
Fil: Coluccio Leskow, Carla. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diaz Zirpolo, Jose Antonio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Costa, Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; Países Bajos
Fil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar Km for both alleles and a slightly higher Vmax for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vacInv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46969
Coluccio Leskow, Carla; Kamenetzky, Laura; Dominguez, Pia Guadalupe; Diaz Zirpolo, Jose Antonio; Obata, Toshihiro; et al.; Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development; Oxford University Press; Journal of Experimental Botany; 67; 14; 7-2016; 4091-4103
0022-0957
1460-2431
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46969
identifier_str_mv Coluccio Leskow, Carla; Kamenetzky, Laura; Dominguez, Pia Guadalupe; Diaz Zirpolo, Jose Antonio; Obata, Toshihiro; et al.; Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development; Oxford University Press; Journal of Experimental Botany; 67; 14; 7-2016; 4091-4103
0022-0957
1460-2431
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/67/14/4091/2197644
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erw185
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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