Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion

Autores
Verdenelli, Romina A.; Dominchin, María F.; Barbero, Florencia Magali; Perez Brandan, Carolina; Aoki, Antonio; Vargas Gil, Silvina; Meriles, José Manuel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The impact of both fungicide and water erosion on soil microbial communities has been little studied, and far less in subhumid-dry climates. To help fill this knowledge gap, we evaluated the influence of two broad-spectrum fungicides applied to soil subjected to different degrees of water erosion on microbial population and function. Soil samples were taken from an experimental site under three water erosion levels: an agricultural land subjected to minimum water erosion by terrace farming (MIN), an agricultural land subjected to moderate water erosion without terrace farming (MOD), and a land with no water erosion control treatment (non-eroded, NON). Three dosages of both carbendazim and iprodione were applied to microcosms, and the responses of microbial community and function were examined after 30-day exposure. Phospholipid fatty acid (PLFA) analysis, soil enzyme activities, and microbial abundances estimated by quantitative real-time PCR (qPCR) were used to evaluate the impact of carbendazim and iprodione fungicides applied at three dosages on microbial community structure and enzyme activities. The lowest values of fluorescein diacetate hydrolase (FDA), dehydrogenase, urease, and glucosidase activities were registered in soil under moderate erosion, while the highest ones were observed in non-eroded soils. Both carbendazim and iprodione decreased C- and P-related activities, even when those fungicides were applied at field rate doses. Thus, phosphomonoesterase, glucosidase, and xylosidase in soil treated with fungicides applied at recommended field rate decreased by 46 %, 61 %, and 34 % compared to soil without fungicide application. The lowest values of PLFA biomarkers for Gram-positive bacteria, Gram-negative bacteria, and arbuscular mycorrhizal fungi were registered in soils treated with the highest dose of both carbendazim and iprodione. Thus, Gram-positive bacteria, Gram-negative bacteria, arbuscular mycorrhizal fungi, and fungal PLFA biomarkers in soils without fungicide application were about 36 %, 3 %, 47 %, and 82 % higher than those soils treated with the highest doses of fungicides, respectively. In addition, the highest doses of fungicide application tended to greater decrease total PLFAs in both non-eroded and minimum eroded compared to moderate eroded soils. Similarly, the response of bacterial and fungal populations to fungicide treatment was more pronounced in non-eroded soils than in eroded soils. This study illustrates how the interactions between fungicides and water erosion levels affect soil enzyme activities, microbial abundances, and microbial community structure in agroecosystems.
Instituto de Patología Vegetal
Fil: Verdenelli, Romina A. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina
Fil: Verdenelli, Romina A. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Dominchin, María F. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina
Fil: Dominchin, María F. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Barbero, Florencia M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina
Fil: Barbero, Florencia M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aoki, Antonio. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Meriles, José M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina
Fil: Meriles, José M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina
Fuente
Applied Soil Ecology 190 : 104984 (October 2023)
Materia
Carbendazim
Iprodione
Enzyme Activity
Water Erosion
Fungicides
Carbendazima
Actividad Enzimática
Erosión Hídrica
Fungicida
PLFA
Microbial Abundance
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18557
Acceder
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oai_identifier_str oai:localhost:20.500.12123/18557
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosionVerdenelli, Romina A.Dominchin, María F.Barbero, Florencia MagaliPerez Brandan, CarolinaAoki, AntonioVargas Gil, SilvinaMeriles, José ManuelCarbendazimIprodioneEnzyme ActivityWater ErosionFungicidesCarbendazimaActividad EnzimáticaErosión HídricaFungicidaPLFAMicrobial AbundanceThe impact of both fungicide and water erosion on soil microbial communities has been little studied, and far less in subhumid-dry climates. To help fill this knowledge gap, we evaluated the influence of two broad-spectrum fungicides applied to soil subjected to different degrees of water erosion on microbial population and function. Soil samples were taken from an experimental site under three water erosion levels: an agricultural land subjected to minimum water erosion by terrace farming (MIN), an agricultural land subjected to moderate water erosion without terrace farming (MOD), and a land with no water erosion control treatment (non-eroded, NON). Three dosages of both carbendazim and iprodione were applied to microcosms, and the responses of microbial community and function were examined after 30-day exposure. Phospholipid fatty acid (PLFA) analysis, soil enzyme activities, and microbial abundances estimated by quantitative real-time PCR (qPCR) were used to evaluate the impact of carbendazim and iprodione fungicides applied at three dosages on microbial community structure and enzyme activities. The lowest values of fluorescein diacetate hydrolase (FDA), dehydrogenase, urease, and glucosidase activities were registered in soil under moderate erosion, while the highest ones were observed in non-eroded soils. Both carbendazim and iprodione decreased C- and P-related activities, even when those fungicides were applied at field rate doses. Thus, phosphomonoesterase, glucosidase, and xylosidase in soil treated with fungicides applied at recommended field rate decreased by 46 %, 61 %, and 34 % compared to soil without fungicide application. The lowest values of PLFA biomarkers for Gram-positive bacteria, Gram-negative bacteria, and arbuscular mycorrhizal fungi were registered in soils treated with the highest dose of both carbendazim and iprodione. Thus, Gram-positive bacteria, Gram-negative bacteria, arbuscular mycorrhizal fungi, and fungal PLFA biomarkers in soils without fungicide application were about 36 %, 3 %, 47 %, and 82 % higher than those soils treated with the highest doses of fungicides, respectively. In addition, the highest doses of fungicide application tended to greater decrease total PLFAs in both non-eroded and minimum eroded compared to moderate eroded soils. Similarly, the response of bacterial and fungal populations to fungicide treatment was more pronounced in non-eroded soils than in eroded soils. This study illustrates how the interactions between fungicides and water erosion levels affect soil enzyme activities, microbial abundances, and microbial community structure in agroecosystems.Instituto de Patología VegetalFil: Verdenelli, Romina A. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; ArgentinaFil: Verdenelli, Romina A. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Dominchin, María F. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; ArgentinaFil: Dominchin, María F. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Barbero, Florencia M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; ArgentinaFil: Barbero, Florencia M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Aoki, Antonio. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Meriles, José M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; ArgentinaFil: Meriles, José M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; ArgentinaElsevier2024-07-18T09:57:18Z2024-07-18T09:57:18Z2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18557https://www.sciencedirect.com/science/article/pii/S09291393230018280929-13931873-0272 (online)https://doi.org/10.1016/j.apsoil.2023.104984Applied Soil Ecology 190 : 104984 (October 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:39Zoai:localhost:20.500.12123/18557instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:40.002INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
title Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
spellingShingle Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
Verdenelli, Romina A.
Carbendazim
Iprodione
Enzyme Activity
Water Erosion
Fungicides
Carbendazima
Actividad Enzimática
Erosión Hídrica
Fungicida
PLFA
Microbial Abundance
title_short Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
title_full Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
title_fullStr Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
title_full_unstemmed Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
title_sort Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
dc.creator.none.fl_str_mv Verdenelli, Romina A.
Dominchin, María F.
Barbero, Florencia Magali
Perez Brandan, Carolina
Aoki, Antonio
Vargas Gil, Silvina
Meriles, José Manuel
author Verdenelli, Romina A.
author_facet Verdenelli, Romina A.
Dominchin, María F.
Barbero, Florencia Magali
Perez Brandan, Carolina
Aoki, Antonio
Vargas Gil, Silvina
Meriles, José Manuel
author_role author
author2 Dominchin, María F.
Barbero, Florencia Magali
Perez Brandan, Carolina
Aoki, Antonio
Vargas Gil, Silvina
Meriles, José Manuel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Carbendazim
Iprodione
Enzyme Activity
Water Erosion
Fungicides
Carbendazima
Actividad Enzimática
Erosión Hídrica
Fungicida
PLFA
Microbial Abundance
topic Carbendazim
Iprodione
Enzyme Activity
Water Erosion
Fungicides
Carbendazima
Actividad Enzimática
Erosión Hídrica
Fungicida
PLFA
Microbial Abundance
dc.description.none.fl_txt_mv The impact of both fungicide and water erosion on soil microbial communities has been little studied, and far less in subhumid-dry climates. To help fill this knowledge gap, we evaluated the influence of two broad-spectrum fungicides applied to soil subjected to different degrees of water erosion on microbial population and function. Soil samples were taken from an experimental site under three water erosion levels: an agricultural land subjected to minimum water erosion by terrace farming (MIN), an agricultural land subjected to moderate water erosion without terrace farming (MOD), and a land with no water erosion control treatment (non-eroded, NON). Three dosages of both carbendazim and iprodione were applied to microcosms, and the responses of microbial community and function were examined after 30-day exposure. Phospholipid fatty acid (PLFA) analysis, soil enzyme activities, and microbial abundances estimated by quantitative real-time PCR (qPCR) were used to evaluate the impact of carbendazim and iprodione fungicides applied at three dosages on microbial community structure and enzyme activities. The lowest values of fluorescein diacetate hydrolase (FDA), dehydrogenase, urease, and glucosidase activities were registered in soil under moderate erosion, while the highest ones were observed in non-eroded soils. Both carbendazim and iprodione decreased C- and P-related activities, even when those fungicides were applied at field rate doses. Thus, phosphomonoesterase, glucosidase, and xylosidase in soil treated with fungicides applied at recommended field rate decreased by 46 %, 61 %, and 34 % compared to soil without fungicide application. The lowest values of PLFA biomarkers for Gram-positive bacteria, Gram-negative bacteria, and arbuscular mycorrhizal fungi were registered in soils treated with the highest dose of both carbendazim and iprodione. Thus, Gram-positive bacteria, Gram-negative bacteria, arbuscular mycorrhizal fungi, and fungal PLFA biomarkers in soils without fungicide application were about 36 %, 3 %, 47 %, and 82 % higher than those soils treated with the highest doses of fungicides, respectively. In addition, the highest doses of fungicide application tended to greater decrease total PLFAs in both non-eroded and minimum eroded compared to moderate eroded soils. Similarly, the response of bacterial and fungal populations to fungicide treatment was more pronounced in non-eroded soils than in eroded soils. This study illustrates how the interactions between fungicides and water erosion levels affect soil enzyme activities, microbial abundances, and microbial community structure in agroecosystems.
Instituto de Patología Vegetal
Fil: Verdenelli, Romina A. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina
Fil: Verdenelli, Romina A. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Dominchin, María F. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina
Fil: Dominchin, María F. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Barbero, Florencia M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina
Fil: Barbero, Florencia M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aoki, Antonio. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Meriles, José M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina
Fil: Meriles, José M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina
description The impact of both fungicide and water erosion on soil microbial communities has been little studied, and far less in subhumid-dry climates. To help fill this knowledge gap, we evaluated the influence of two broad-spectrum fungicides applied to soil subjected to different degrees of water erosion on microbial population and function. Soil samples were taken from an experimental site under three water erosion levels: an agricultural land subjected to minimum water erosion by terrace farming (MIN), an agricultural land subjected to moderate water erosion without terrace farming (MOD), and a land with no water erosion control treatment (non-eroded, NON). Three dosages of both carbendazim and iprodione were applied to microcosms, and the responses of microbial community and function were examined after 30-day exposure. Phospholipid fatty acid (PLFA) analysis, soil enzyme activities, and microbial abundances estimated by quantitative real-time PCR (qPCR) were used to evaluate the impact of carbendazim and iprodione fungicides applied at three dosages on microbial community structure and enzyme activities. The lowest values of fluorescein diacetate hydrolase (FDA), dehydrogenase, urease, and glucosidase activities were registered in soil under moderate erosion, while the highest ones were observed in non-eroded soils. Both carbendazim and iprodione decreased C- and P-related activities, even when those fungicides were applied at field rate doses. Thus, phosphomonoesterase, glucosidase, and xylosidase in soil treated with fungicides applied at recommended field rate decreased by 46 %, 61 %, and 34 % compared to soil without fungicide application. The lowest values of PLFA biomarkers for Gram-positive bacteria, Gram-negative bacteria, and arbuscular mycorrhizal fungi were registered in soils treated with the highest dose of both carbendazim and iprodione. Thus, Gram-positive bacteria, Gram-negative bacteria, arbuscular mycorrhizal fungi, and fungal PLFA biomarkers in soils without fungicide application were about 36 %, 3 %, 47 %, and 82 % higher than those soils treated with the highest doses of fungicides, respectively. In addition, the highest doses of fungicide application tended to greater decrease total PLFAs in both non-eroded and minimum eroded compared to moderate eroded soils. Similarly, the response of bacterial and fungal populations to fungicide treatment was more pronounced in non-eroded soils than in eroded soils. This study illustrates how the interactions between fungicides and water erosion levels affect soil enzyme activities, microbial abundances, and microbial community structure in agroecosystems.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
2024-07-18T09:57:18Z
2024-07-18T09:57:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18557
https://www.sciencedirect.com/science/article/pii/S0929139323001828
0929-1393
1873-0272 (online)
https://doi.org/10.1016/j.apsoil.2023.104984
url http://hdl.handle.net/20.500.12123/18557
https://www.sciencedirect.com/science/article/pii/S0929139323001828
https://doi.org/10.1016/j.apsoil.2023.104984
identifier_str_mv 0929-1393
1873-0272 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Applied Soil Ecology 190 : 104984 (October 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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