Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis

Autores
Salomon, Virginia María; Gianni De Carvalho, Katia; Arroyo, Florencia; Maldonado, Luis Maria; Gennari, Gerardo Pablo; Vera, Nancy Roxana; Romero, Cintia Mariana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The products of stingless bees have been used in traditional medicine. These products have gained economic potential not only for their historical valuation but also to produce added value related to the knowledge of the qualities of their indigenous microbiota. The isolates from honey and pollen of Scaptotrigona jujuyensis, a stingless bee from Northern Argentina were studied. These were able to produce hydrolytic enzymes: protease, amylase, xylanase, cellulose, and lipase, and growing in bile salts. The isolate 4A was identified as a Bacillus sp. and was able to produce extracellular exopolysaccharides (EPS). The carbohydrate composition of EPS consisted predominantly of fructose (44.6%). Structural characterization of EPS using Fourier-transform infrared spectroscopy (FTIR) showed high similarity with levan. The EPS showed antimicrobial activity and the capacity to form emulsion hydrogels with omega-3 polyunsaturated fatty acids (PUFA) from ray liver oil and chia oil. The prebiotic property of Lactobacillus casei was evaluated with EPS and its mix with omega-3 PUFA. L. casei, showed better growth. Thus, an EPS with emulsifying hydrogel capacity and prebiotic activity was produced from the native microbial flora present in the honey of a stingless bee, which could be an added-value product of the S. jujuyensis colony used as a new nutraceutical.
EEA Famaillá
Fil: Salomon, Virginia María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Gianni De Carvalho, Katia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.
Fil: Arroyo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Maldonado, Luis Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Gennari, Gerardo Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Vera, Nancy R. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Farmacoquímica; Argentina
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Romero, Cintia Mariana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Farmacoquímica; Argentina
Fuente
Food Bioscience 42 : 101077 (August 2021)
Materia
Melipona
Biopolímeros
Productos de la Colmena
Propiedades Antimicrobianas
Bacillus
Biopolymers
Hive Products
Antimicrobial Properties
Abejas sin Aguijón
Scaptotrigona jujuyensis
Stingless Bees
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9242
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9242
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network_name_str INTA Digital (INTA)
spelling Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensisSalomon, Virginia MaríaGianni De Carvalho, KatiaArroyo, FlorenciaMaldonado, Luis MariaGennari, Gerardo PabloVera, Nancy RoxanaRomero, Cintia MarianaMeliponaBiopolímerosProductos de la ColmenaPropiedades AntimicrobianasBacillusBiopolymersHive ProductsAntimicrobial PropertiesAbejas sin AguijónScaptotrigona jujuyensisStingless BeesThe products of stingless bees have been used in traditional medicine. These products have gained economic potential not only for their historical valuation but also to produce added value related to the knowledge of the qualities of their indigenous microbiota. The isolates from honey and pollen of Scaptotrigona jujuyensis, a stingless bee from Northern Argentina were studied. These were able to produce hydrolytic enzymes: protease, amylase, xylanase, cellulose, and lipase, and growing in bile salts. The isolate 4A was identified as a Bacillus sp. and was able to produce extracellular exopolysaccharides (EPS). The carbohydrate composition of EPS consisted predominantly of fructose (44.6%). Structural characterization of EPS using Fourier-transform infrared spectroscopy (FTIR) showed high similarity with levan. The EPS showed antimicrobial activity and the capacity to form emulsion hydrogels with omega-3 polyunsaturated fatty acids (PUFA) from ray liver oil and chia oil. The prebiotic property of Lactobacillus casei was evaluated with EPS and its mix with omega-3 PUFA. L. casei, showed better growth. Thus, an EPS with emulsifying hydrogel capacity and prebiotic activity was produced from the native microbial flora present in the honey of a stingless bee, which could be an added-value product of the S. jujuyensis colony used as a new nutraceutical.EEA FamailláFil: Salomon, Virginia María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Gianni De Carvalho, Katia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.Fil: Arroyo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Maldonado, Luis Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Gennari, Gerardo Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Vera, Nancy R. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Farmacoquímica; ArgentinaFil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Romero, Cintia Mariana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Farmacoquímica; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2022-04-302021-04-30T17:23:28Z2021-04-30T17:23:28Z2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9242https://www.sciencedirect.com/science/article/abs/pii/S22124292210020292212-4292https://doi.org/10.1016/j.fbio.2021.101077Food Bioscience 42 : 101077 (August 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNAPI-1112043/AR./Estrategias para agregar valor a la producción apícola argentina.info:eu-repograntAgreement/INTA/PNAPI-1112044/AR./Gestión y mejora del servicio ambiental de polinizacióninfo:eu-repo/semantics/openAccess2025-09-29T13:45:12Zoai:localhost:20.500.12123/9242instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:12.647INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis
title Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis
spellingShingle Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis
Salomon, Virginia María
Melipona
Biopolímeros
Productos de la Colmena
Propiedades Antimicrobianas
Bacillus
Biopolymers
Hive Products
Antimicrobial Properties
Abejas sin Aguijón
Scaptotrigona jujuyensis
Stingless Bees
title_short Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis
title_full Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis
title_fullStr Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis
title_full_unstemmed Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis
title_sort Biopolymer production by bacteria isolated from native stingless bee honey, Scaptotrigona jujuyensis
dc.creator.none.fl_str_mv Salomon, Virginia María
Gianni De Carvalho, Katia
Arroyo, Florencia
Maldonado, Luis Maria
Gennari, Gerardo Pablo
Vera, Nancy Roxana
Romero, Cintia Mariana
author Salomon, Virginia María
author_facet Salomon, Virginia María
Gianni De Carvalho, Katia
Arroyo, Florencia
Maldonado, Luis Maria
Gennari, Gerardo Pablo
Vera, Nancy Roxana
Romero, Cintia Mariana
author_role author
author2 Gianni De Carvalho, Katia
Arroyo, Florencia
Maldonado, Luis Maria
Gennari, Gerardo Pablo
Vera, Nancy Roxana
Romero, Cintia Mariana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Melipona
Biopolímeros
Productos de la Colmena
Propiedades Antimicrobianas
Bacillus
Biopolymers
Hive Products
Antimicrobial Properties
Abejas sin Aguijón
Scaptotrigona jujuyensis
Stingless Bees
topic Melipona
Biopolímeros
Productos de la Colmena
Propiedades Antimicrobianas
Bacillus
Biopolymers
Hive Products
Antimicrobial Properties
Abejas sin Aguijón
Scaptotrigona jujuyensis
Stingless Bees
dc.description.none.fl_txt_mv The products of stingless bees have been used in traditional medicine. These products have gained economic potential not only for their historical valuation but also to produce added value related to the knowledge of the qualities of their indigenous microbiota. The isolates from honey and pollen of Scaptotrigona jujuyensis, a stingless bee from Northern Argentina were studied. These were able to produce hydrolytic enzymes: protease, amylase, xylanase, cellulose, and lipase, and growing in bile salts. The isolate 4A was identified as a Bacillus sp. and was able to produce extracellular exopolysaccharides (EPS). The carbohydrate composition of EPS consisted predominantly of fructose (44.6%). Structural characterization of EPS using Fourier-transform infrared spectroscopy (FTIR) showed high similarity with levan. The EPS showed antimicrobial activity and the capacity to form emulsion hydrogels with omega-3 polyunsaturated fatty acids (PUFA) from ray liver oil and chia oil. The prebiotic property of Lactobacillus casei was evaluated with EPS and its mix with omega-3 PUFA. L. casei, showed better growth. Thus, an EPS with emulsifying hydrogel capacity and prebiotic activity was produced from the native microbial flora present in the honey of a stingless bee, which could be an added-value product of the S. jujuyensis colony used as a new nutraceutical.
EEA Famaillá
Fil: Salomon, Virginia María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Gianni De Carvalho, Katia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.
Fil: Arroyo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Maldonado, Luis Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Gennari, Gerardo Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Vera, Nancy R. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Farmacoquímica; Argentina
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Romero, Cintia Mariana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Farmacoquímica; Argentina
description The products of stingless bees have been used in traditional medicine. These products have gained economic potential not only for their historical valuation but also to produce added value related to the knowledge of the qualities of their indigenous microbiota. The isolates from honey and pollen of Scaptotrigona jujuyensis, a stingless bee from Northern Argentina were studied. These were able to produce hydrolytic enzymes: protease, amylase, xylanase, cellulose, and lipase, and growing in bile salts. The isolate 4A was identified as a Bacillus sp. and was able to produce extracellular exopolysaccharides (EPS). The carbohydrate composition of EPS consisted predominantly of fructose (44.6%). Structural characterization of EPS using Fourier-transform infrared spectroscopy (FTIR) showed high similarity with levan. The EPS showed antimicrobial activity and the capacity to form emulsion hydrogels with omega-3 polyunsaturated fatty acids (PUFA) from ray liver oil and chia oil. The prebiotic property of Lactobacillus casei was evaluated with EPS and its mix with omega-3 PUFA. L. casei, showed better growth. Thus, an EPS with emulsifying hydrogel capacity and prebiotic activity was produced from the native microbial flora present in the honey of a stingless bee, which could be an added-value product of the S. jujuyensis colony used as a new nutraceutical.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-30T17:23:28Z
2021-04-30T17:23:28Z
2021-04
info:eu-repo/date/embargoEnd/2022-04-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9242
https://www.sciencedirect.com/science/article/abs/pii/S2212429221002029
2212-4292
https://doi.org/10.1016/j.fbio.2021.101077
url http://hdl.handle.net/20.500.12123/9242
https://www.sciencedirect.com/science/article/abs/pii/S2212429221002029
https://doi.org/10.1016/j.fbio.2021.101077
identifier_str_mv 2212-4292
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNAPI-1112043/AR./Estrategias para agregar valor a la producción apícola argentina.
info:eu-repograntAgreement/INTA/PNAPI-1112044/AR./Gestión y mejora del servicio ambiental de polinización
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Food Bioscience 42 : 101077 (August 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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