Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis
- Autores
- Wieland, Nina; Figueiredo, Daniel; Mol, Hans; González, Neus; Abrantes, Nelson; Aparicio, Virginia Carolina; Campos, Isabel; Contreras, Josefina; Francisco, Alcon; Glavan, Matjaž; Blagus, Tanja; Dolžan, Vita; Harkes, Paula; Nørgaard, Trine; Schlünssen, Vivi; Pasković, Igor; Polić Pasković, Marija; Graumans, Martien; Ragas, Ad; Russel, Frans; Scheepers, Paul
- Año de publicación
- 2026
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Dietary uptake is estimated using residue data from food commodities and predicting the effect of food processing, such as peeling. The aim of this study was to explore the value of the analysis of duplicate portions supplemented by the analysis of urinary metabolites. Forty-three participants, consuming organic and nonorganic diets collected a duplicate portion of food and beverages for 24 h. Urine was collected up to 36 h to account for metabolism and prolonged excretion of metabolites. Pesticide residues were analysed in food portions and urine samples using LC-MS/MS and GC-MS/MS. Multiple pesticide residues were detected per food portion. Out of 183 pesticides, 86 were detected in the diet. Glyphosate and AMPA were detected in 42 % and 30 % of all urine samples, respectively, while detection in the diet was low. A positive relationship between dietary intake and urinary excretion was found for 2,4-D and MCPA. No diet-urine relationship was observed for glyphosate and AMPA, indicating contribution from external routes of exposure. Hazard index (HI) indicated no exposure above the acceptable daily intake (ADI) for all participants. This study demonstrates how DPA combined with urine analysis gives insight into contribution of diet to total pesticide exposure compared to standard monitoring.
EEA Balcarce
Fil: Wieland, Nina. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos
Fil: Figueiredo, Daniel. Utrecht University. Institute for Risk Assessment Sciences (IRAS); Países Bajos
Fil: Mol, Hans. Wageningen University & Research. Wageningen Food Safety Research (WFSR); Países Bajos
Fil: González, Neus. Universitat Rovira i Virgili. Institut d’Investigació Sanitària Pere Virgili (IISPV). Laboratory of Toxicology and Environmental Health; España
Fil: Abrantes, Nelson. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; Portugal
Fil: Aparicio, Virginia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Campos, Isabel. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; Portugal
Fil: Contreras, Josefina. Universidad Politécnica de Cartagena. Agricultural Engineering School; España
Fil: Francisco, Alcon. Universidad Politécnica de Cartagena. Agricultural Engineering School; España
Fil: Glavan, Matjaž. University of Ljubljana. Biotechnical Faculty. Agronomy Department; Eslovenia
Fil: Blagus, Tanja. University of Ljubljana. Faculty of Medicine. Institute of Biochemistry and Molecular Genetics. Pharmacogenetics Laboratory; Eslovenia
Fil. Dolžan, Vita. University of Ljubljana. Faculty of Medicine. Institute of Biochemistry and Molecular Genetics. Pharmacogenetics Laboratory; Eslovenia
Fil: Harkes, Paula. Wageningen University & Research (WUR). Soil Physics and Land Management Group; Países Bajos
Fil: Nørgaard, Trine. Aarhus University. Danish Ramazzini Centre. Department of Public Health; Dinamarca
Fil: Schlünssen, Vivi. Aarhus University. Danish Ramazzini Centre. Department of Public Health; Dinamarca
Fil: Pasković, Igor. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; Croacia
Fil: Polić Pasković, Marija. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; Croacia
Fil: Graumans, Martien. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos
Fil: Ragas, Ad. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos
Fil: Russel, Frans. Radboud University. Division of Pharmacology and Toxicology. Department of Pharmacy; Países Bajos
Fil: Scheepers, Paul. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos - Fuente
- Regulatory Toxicology and Pharmacology 164 : 105972. (January 2026)
- Materia
-
Plaguicidas
Dieta
Orinación
Ración Diaria Aceptable
Pesticides
Diet
Urination
Acceptable Daily Intake
Dietary Pesticide
Urinary Excretion
Dietary Exposure
Duplicate Portion Analysis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/24996
Ver los metadatos del registro completo
| id |
INTADig_b739cd3cb1f20bc51a92d03474585ffd |
|---|---|
| oai_identifier_str |
oai:localhost:20.500.12123/24996 |
| network_acronym_str |
INTADig |
| repository_id_str |
l |
| network_name_str |
INTA Digital (INTA) |
| spelling |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysisWieland, NinaFigueiredo, DanielMol, HansGonzález, NeusAbrantes, NelsonAparicio, Virginia CarolinaCampos, IsabelContreras, JosefinaFrancisco, AlconGlavan, MatjažBlagus, TanjaDolžan, VitaHarkes, PaulaNørgaard, TrineSchlünssen, ViviPasković, IgorPolić Pasković, MarijaGraumans, MartienRagas, AdRussel, FransScheepers, PaulPlaguicidasDietaOrinaciónRación Diaria AceptablePesticidesDietUrinationAcceptable Daily IntakeDietary PesticideUrinary ExcretionDietary ExposureDuplicate Portion AnalysisDietary uptake is estimated using residue data from food commodities and predicting the effect of food processing, such as peeling. The aim of this study was to explore the value of the analysis of duplicate portions supplemented by the analysis of urinary metabolites. Forty-three participants, consuming organic and nonorganic diets collected a duplicate portion of food and beverages for 24 h. Urine was collected up to 36 h to account for metabolism and prolonged excretion of metabolites. Pesticide residues were analysed in food portions and urine samples using LC-MS/MS and GC-MS/MS. Multiple pesticide residues were detected per food portion. Out of 183 pesticides, 86 were detected in the diet. Glyphosate and AMPA were detected in 42 % and 30 % of all urine samples, respectively, while detection in the diet was low. A positive relationship between dietary intake and urinary excretion was found for 2,4-D and MCPA. No diet-urine relationship was observed for glyphosate and AMPA, indicating contribution from external routes of exposure. Hazard index (HI) indicated no exposure above the acceptable daily intake (ADI) for all participants. This study demonstrates how DPA combined with urine analysis gives insight into contribution of diet to total pesticide exposure compared to standard monitoring.EEA BalcarceFil: Wieland, Nina. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países BajosFil: Figueiredo, Daniel. Utrecht University. Institute for Risk Assessment Sciences (IRAS); Países BajosFil: Mol, Hans. Wageningen University & Research. Wageningen Food Safety Research (WFSR); Países BajosFil: González, Neus. Universitat Rovira i Virgili. Institut d’Investigació Sanitària Pere Virgili (IISPV). Laboratory of Toxicology and Environmental Health; EspañaFil: Abrantes, Nelson. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; PortugalFil: Aparicio, Virginia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Campos, Isabel. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; PortugalFil: Contreras, Josefina. Universidad Politécnica de Cartagena. Agricultural Engineering School; EspañaFil: Francisco, Alcon. Universidad Politécnica de Cartagena. Agricultural Engineering School; EspañaFil: Glavan, Matjaž. University of Ljubljana. Biotechnical Faculty. Agronomy Department; EsloveniaFil: Blagus, Tanja. University of Ljubljana. Faculty of Medicine. Institute of Biochemistry and Molecular Genetics. Pharmacogenetics Laboratory; EsloveniaFil. Dolžan, Vita. University of Ljubljana. Faculty of Medicine. Institute of Biochemistry and Molecular Genetics. Pharmacogenetics Laboratory; EsloveniaFil: Harkes, Paula. Wageningen University & Research (WUR). Soil Physics and Land Management Group; Países BajosFil: Nørgaard, Trine. Aarhus University. Danish Ramazzini Centre. Department of Public Health; DinamarcaFil: Schlünssen, Vivi. Aarhus University. Danish Ramazzini Centre. Department of Public Health; DinamarcaFil: Pasković, Igor. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; CroaciaFil: Polić Pasković, Marija. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; CroaciaFil: Graumans, Martien. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países BajosFil: Ragas, Ad. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países BajosFil: Russel, Frans. Radboud University. Division of Pharmacology and Toxicology. Department of Pharmacy; Países BajosFil: Scheepers, Paul. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países BajosElsevier2026-01-16T14:52:34Z2026-01-16T14:52:34Z2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24996https://www.sciencedirect.com/science/article/pii/S0273230025002041?via%3Dihub0273-2300 (impreso)1096-0295 (online)https://doi.org/10.1016/j.yrtph.2025.105972Regulatory Toxicology and Pharmacology 164 : 105972. (January 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-05T12:54:27Zoai:localhost:20.500.12123/24996instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-05 12:54:27.442INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis |
| title |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis |
| spellingShingle |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis Wieland, Nina Plaguicidas Dieta Orinación Ración Diaria Aceptable Pesticides Diet Urination Acceptable Daily Intake Dietary Pesticide Urinary Excretion Dietary Exposure Duplicate Portion Analysis |
| title_short |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis |
| title_full |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis |
| title_fullStr |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis |
| title_full_unstemmed |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis |
| title_sort |
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis |
| dc.creator.none.fl_str_mv |
Wieland, Nina Figueiredo, Daniel Mol, Hans González, Neus Abrantes, Nelson Aparicio, Virginia Carolina Campos, Isabel Contreras, Josefina Francisco, Alcon Glavan, Matjaž Blagus, Tanja Dolžan, Vita Harkes, Paula Nørgaard, Trine Schlünssen, Vivi Pasković, Igor Polić Pasković, Marija Graumans, Martien Ragas, Ad Russel, Frans Scheepers, Paul |
| author |
Wieland, Nina |
| author_facet |
Wieland, Nina Figueiredo, Daniel Mol, Hans González, Neus Abrantes, Nelson Aparicio, Virginia Carolina Campos, Isabel Contreras, Josefina Francisco, Alcon Glavan, Matjaž Blagus, Tanja Dolžan, Vita Harkes, Paula Nørgaard, Trine Schlünssen, Vivi Pasković, Igor Polić Pasković, Marija Graumans, Martien Ragas, Ad Russel, Frans Scheepers, Paul |
| author_role |
author |
| author2 |
Figueiredo, Daniel Mol, Hans González, Neus Abrantes, Nelson Aparicio, Virginia Carolina Campos, Isabel Contreras, Josefina Francisco, Alcon Glavan, Matjaž Blagus, Tanja Dolžan, Vita Harkes, Paula Nørgaard, Trine Schlünssen, Vivi Pasković, Igor Polić Pasković, Marija Graumans, Martien Ragas, Ad Russel, Frans Scheepers, Paul |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Plaguicidas Dieta Orinación Ración Diaria Aceptable Pesticides Diet Urination Acceptable Daily Intake Dietary Pesticide Urinary Excretion Dietary Exposure Duplicate Portion Analysis |
| topic |
Plaguicidas Dieta Orinación Ración Diaria Aceptable Pesticides Diet Urination Acceptable Daily Intake Dietary Pesticide Urinary Excretion Dietary Exposure Duplicate Portion Analysis |
| dc.description.none.fl_txt_mv |
Dietary uptake is estimated using residue data from food commodities and predicting the effect of food processing, such as peeling. The aim of this study was to explore the value of the analysis of duplicate portions supplemented by the analysis of urinary metabolites. Forty-three participants, consuming organic and nonorganic diets collected a duplicate portion of food and beverages for 24 h. Urine was collected up to 36 h to account for metabolism and prolonged excretion of metabolites. Pesticide residues were analysed in food portions and urine samples using LC-MS/MS and GC-MS/MS. Multiple pesticide residues were detected per food portion. Out of 183 pesticides, 86 were detected in the diet. Glyphosate and AMPA were detected in 42 % and 30 % of all urine samples, respectively, while detection in the diet was low. A positive relationship between dietary intake and urinary excretion was found for 2,4-D and MCPA. No diet-urine relationship was observed for glyphosate and AMPA, indicating contribution from external routes of exposure. Hazard index (HI) indicated no exposure above the acceptable daily intake (ADI) for all participants. This study demonstrates how DPA combined with urine analysis gives insight into contribution of diet to total pesticide exposure compared to standard monitoring. EEA Balcarce Fil: Wieland, Nina. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos Fil: Figueiredo, Daniel. Utrecht University. Institute for Risk Assessment Sciences (IRAS); Países Bajos Fil: Mol, Hans. Wageningen University & Research. Wageningen Food Safety Research (WFSR); Países Bajos Fil: González, Neus. Universitat Rovira i Virgili. Institut d’Investigació Sanitària Pere Virgili (IISPV). Laboratory of Toxicology and Environmental Health; España Fil: Abrantes, Nelson. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; Portugal Fil: Aparicio, Virginia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Campos, Isabel. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; Portugal Fil: Contreras, Josefina. Universidad Politécnica de Cartagena. Agricultural Engineering School; España Fil: Francisco, Alcon. Universidad Politécnica de Cartagena. Agricultural Engineering School; España Fil: Glavan, Matjaž. University of Ljubljana. Biotechnical Faculty. Agronomy Department; Eslovenia Fil: Blagus, Tanja. University of Ljubljana. Faculty of Medicine. Institute of Biochemistry and Molecular Genetics. Pharmacogenetics Laboratory; Eslovenia Fil. Dolžan, Vita. University of Ljubljana. Faculty of Medicine. Institute of Biochemistry and Molecular Genetics. Pharmacogenetics Laboratory; Eslovenia Fil: Harkes, Paula. Wageningen University & Research (WUR). Soil Physics and Land Management Group; Países Bajos Fil: Nørgaard, Trine. Aarhus University. Danish Ramazzini Centre. Department of Public Health; Dinamarca Fil: Schlünssen, Vivi. Aarhus University. Danish Ramazzini Centre. Department of Public Health; Dinamarca Fil: Pasković, Igor. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; Croacia Fil: Polić Pasković, Marija. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; Croacia Fil: Graumans, Martien. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos Fil: Ragas, Ad. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos Fil: Russel, Frans. Radboud University. Division of Pharmacology and Toxicology. Department of Pharmacy; Países Bajos Fil: Scheepers, Paul. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos |
| description |
Dietary uptake is estimated using residue data from food commodities and predicting the effect of food processing, such as peeling. The aim of this study was to explore the value of the analysis of duplicate portions supplemented by the analysis of urinary metabolites. Forty-three participants, consuming organic and nonorganic diets collected a duplicate portion of food and beverages for 24 h. Urine was collected up to 36 h to account for metabolism and prolonged excretion of metabolites. Pesticide residues were analysed in food portions and urine samples using LC-MS/MS and GC-MS/MS. Multiple pesticide residues were detected per food portion. Out of 183 pesticides, 86 were detected in the diet. Glyphosate and AMPA were detected in 42 % and 30 % of all urine samples, respectively, while detection in the diet was low. A positive relationship between dietary intake and urinary excretion was found for 2,4-D and MCPA. No diet-urine relationship was observed for glyphosate and AMPA, indicating contribution from external routes of exposure. Hazard index (HI) indicated no exposure above the acceptable daily intake (ADI) for all participants. This study demonstrates how DPA combined with urine analysis gives insight into contribution of diet to total pesticide exposure compared to standard monitoring. |
| publishDate |
2026 |
| dc.date.none.fl_str_mv |
2026-01-16T14:52:34Z 2026-01-16T14:52:34Z 2026-01 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/24996 https://www.sciencedirect.com/science/article/pii/S0273230025002041?via%3Dihub 0273-2300 (impreso) 1096-0295 (online) https://doi.org/10.1016/j.yrtph.2025.105972 |
| url |
http://hdl.handle.net/20.500.12123/24996 https://www.sciencedirect.com/science/article/pii/S0273230025002041?via%3Dihub https://doi.org/10.1016/j.yrtph.2025.105972 |
| identifier_str_mv |
0273-2300 (impreso) 1096-0295 (online) |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
Regulatory Toxicology and Pharmacology 164 : 105972. (January 2026) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
| reponame_str |
INTA Digital (INTA) |
| collection |
INTA Digital (INTA) |
| instname_str |
Instituto Nacional de Tecnología Agropecuaria |
| repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
| repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
| _version_ |
1856302448680894464 |
| score |
13.106097 |