In vivo neutralization of bee venom lethality by IgY antibodies

Autores
Leiva, Carlos Leónidas; Geoghegan, Patricia; Lammer, Mónica; Cangelosi, Adriana; Mariconda, Virginia; Celi Castillo, Ana Beatriz; Brero, Luisa; Chacana, Pablo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings.
Instituto de Patobiología
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Molecular Immunology 135 : 183-190 (Julio 2021)
Materia
Apis mellifera
Veneno de Abeja
Anticuerpos
Yema de Huevo
Experimentación in Vivo
Efectos Letales
Neutralización
Bee Venoms
Antibodies
Egg Yolk
In vivo Experimentation
Lethal Effects
Neutralization
IgY Antibodies
Anticuerpos IgY
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9280

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network_name_str INTA Digital (INTA)
spelling In vivo neutralization of bee venom lethality by IgY antibodiesLeiva, Carlos LeónidasGeoghegan, PatriciaLammer, MónicaCangelosi, AdrianaMariconda, VirginiaCeli Castillo, Ana BeatrizBrero, LuisaChacana, PabloApis melliferaVeneno de AbejaAnticuerposYema de HuevoExperimentación in VivoEfectos LetalesNeutralizaciónBee VenomsAntibodiesEgg YolkIn vivo ExperimentationLethal EffectsNeutralizationIgY AntibodiesAnticuerpos IgYBee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings.Instituto de PatobiologíaFil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2021-05-05T16:38:06Z2021-05-05T16:38:06Z2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9280https://www.sciencedirect.com/science/article/pii/S01615890210013221872-9142https://doi.org/10.1016/j.molimm.2021.04.015Molecular Immunology 135 : 183-190 (Julio 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:04Zoai:localhost:20.500.12123/9280instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:05.395INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vivo neutralization of bee venom lethality by IgY antibodies
title In vivo neutralization of bee venom lethality by IgY antibodies
spellingShingle In vivo neutralization of bee venom lethality by IgY antibodies
Leiva, Carlos Leónidas
Apis mellifera
Veneno de Abeja
Anticuerpos
Yema de Huevo
Experimentación in Vivo
Efectos Letales
Neutralización
Bee Venoms
Antibodies
Egg Yolk
In vivo Experimentation
Lethal Effects
Neutralization
IgY Antibodies
Anticuerpos IgY
title_short In vivo neutralization of bee venom lethality by IgY antibodies
title_full In vivo neutralization of bee venom lethality by IgY antibodies
title_fullStr In vivo neutralization of bee venom lethality by IgY antibodies
title_full_unstemmed In vivo neutralization of bee venom lethality by IgY antibodies
title_sort In vivo neutralization of bee venom lethality by IgY antibodies
dc.creator.none.fl_str_mv Leiva, Carlos Leónidas
Geoghegan, Patricia
Lammer, Mónica
Cangelosi, Adriana
Mariconda, Virginia
Celi Castillo, Ana Beatriz
Brero, Luisa
Chacana, Pablo
author Leiva, Carlos Leónidas
author_facet Leiva, Carlos Leónidas
Geoghegan, Patricia
Lammer, Mónica
Cangelosi, Adriana
Mariconda, Virginia
Celi Castillo, Ana Beatriz
Brero, Luisa
Chacana, Pablo
author_role author
author2 Geoghegan, Patricia
Lammer, Mónica
Cangelosi, Adriana
Mariconda, Virginia
Celi Castillo, Ana Beatriz
Brero, Luisa
Chacana, Pablo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apis mellifera
Veneno de Abeja
Anticuerpos
Yema de Huevo
Experimentación in Vivo
Efectos Letales
Neutralización
Bee Venoms
Antibodies
Egg Yolk
In vivo Experimentation
Lethal Effects
Neutralization
IgY Antibodies
Anticuerpos IgY
topic Apis mellifera
Veneno de Abeja
Anticuerpos
Yema de Huevo
Experimentación in Vivo
Efectos Letales
Neutralización
Bee Venoms
Antibodies
Egg Yolk
In vivo Experimentation
Lethal Effects
Neutralization
IgY Antibodies
Anticuerpos IgY
dc.description.none.fl_txt_mv Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings.
Instituto de Patobiología
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-05T16:38:06Z
2021-05-05T16:38:06Z
2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9280
https://www.sciencedirect.com/science/article/pii/S0161589021001322
1872-9142
https://doi.org/10.1016/j.molimm.2021.04.015
url http://hdl.handle.net/20.500.12123/9280
https://www.sciencedirect.com/science/article/pii/S0161589021001322
https://doi.org/10.1016/j.molimm.2021.04.015
identifier_str_mv 1872-9142
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Molecular Immunology 135 : 183-190 (Julio 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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