In vivo neutralization of bee venom lethality by IgY antibodies
- Autores
- Leiva, Carlos Leónidas; Geoghegan, Patricia; Lammer, Mónica; Cangelosi, Adriana; Mariconda, Virginia; Celi Castillo, Ana Beatriz; Brero, Luisa; Chacana, Pablo
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings.
Instituto de Patobiología
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Molecular Immunology 135 : 183-190 (Julio 2021)
- Materia
-
Apis mellifera
Veneno de Abeja
Anticuerpos
Yema de Huevo
Experimentación in Vivo
Efectos Letales
Neutralización
Bee Venoms
Antibodies
Egg Yolk
In vivo Experimentation
Lethal Effects
Neutralization
IgY Antibodies
Anticuerpos IgY - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/9280
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In vivo neutralization of bee venom lethality by IgY antibodiesLeiva, Carlos LeónidasGeoghegan, PatriciaLammer, MónicaCangelosi, AdrianaMariconda, VirginiaCeli Castillo, Ana BeatrizBrero, LuisaChacana, PabloApis melliferaVeneno de AbejaAnticuerposYema de HuevoExperimentación in VivoEfectos LetalesNeutralizaciónBee VenomsAntibodiesEgg YolkIn vivo ExperimentationLethal EffectsNeutralizationIgY AntibodiesAnticuerpos IgYBee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings.Instituto de PatobiologíaFil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2021-05-05T16:38:06Z2021-05-05T16:38:06Z2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9280https://www.sciencedirect.com/science/article/pii/S01615890210013221872-9142https://doi.org/10.1016/j.molimm.2021.04.015Molecular Immunology 135 : 183-190 (Julio 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:04Zoai:localhost:20.500.12123/9280instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:05.395INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
In vivo neutralization of bee venom lethality by IgY antibodies |
title |
In vivo neutralization of bee venom lethality by IgY antibodies |
spellingShingle |
In vivo neutralization of bee venom lethality by IgY antibodies Leiva, Carlos Leónidas Apis mellifera Veneno de Abeja Anticuerpos Yema de Huevo Experimentación in Vivo Efectos Letales Neutralización Bee Venoms Antibodies Egg Yolk In vivo Experimentation Lethal Effects Neutralization IgY Antibodies Anticuerpos IgY |
title_short |
In vivo neutralization of bee venom lethality by IgY antibodies |
title_full |
In vivo neutralization of bee venom lethality by IgY antibodies |
title_fullStr |
In vivo neutralization of bee venom lethality by IgY antibodies |
title_full_unstemmed |
In vivo neutralization of bee venom lethality by IgY antibodies |
title_sort |
In vivo neutralization of bee venom lethality by IgY antibodies |
dc.creator.none.fl_str_mv |
Leiva, Carlos Leónidas Geoghegan, Patricia Lammer, Mónica Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Brero, Luisa Chacana, Pablo |
author |
Leiva, Carlos Leónidas |
author_facet |
Leiva, Carlos Leónidas Geoghegan, Patricia Lammer, Mónica Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Brero, Luisa Chacana, Pablo |
author_role |
author |
author2 |
Geoghegan, Patricia Lammer, Mónica Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Brero, Luisa Chacana, Pablo |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Apis mellifera Veneno de Abeja Anticuerpos Yema de Huevo Experimentación in Vivo Efectos Letales Neutralización Bee Venoms Antibodies Egg Yolk In vivo Experimentation Lethal Effects Neutralization IgY Antibodies Anticuerpos IgY |
topic |
Apis mellifera Veneno de Abeja Anticuerpos Yema de Huevo Experimentación in Vivo Efectos Letales Neutralización Bee Venoms Antibodies Egg Yolk In vivo Experimentation Lethal Effects Neutralization IgY Antibodies Anticuerpos IgY |
dc.description.none.fl_txt_mv |
Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings. Instituto de Patobiología Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-05T16:38:06Z 2021-05-05T16:38:06Z 2021-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/9280 https://www.sciencedirect.com/science/article/pii/S0161589021001322 1872-9142 https://doi.org/10.1016/j.molimm.2021.04.015 |
url |
http://hdl.handle.net/20.500.12123/9280 https://www.sciencedirect.com/science/article/pii/S0161589021001322 https://doi.org/10.1016/j.molimm.2021.04.015 |
identifier_str_mv |
1872-9142 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Molecular Immunology 135 : 183-190 (Julio 2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1846143534715895808 |
score |
12.712165 |