Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period

Autores
Spetter, Lucas Maximiliano; Louge Uriarte, Enrique Leopoldo; Armendano, Joaquín Ignacio; Morrell, Eleonora Lidia; Canton, German Jose; Verna, Andrea Elizabeth; Dorsch, Matías; Pereyra, Susana Beatriz; Odeon, Anselmo Carlos; Saliki, Jeremiah; González Altamiranda, Erika
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Detection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. Additionally, genetic, serological, and pathological analyses were conducted in order to characterize BVDV strains of fetal origin. Samples from 95 selected cases from 1997 to 2018 were analyzed by antigen-capture ELISA (AgELISA), nested RT-PCR (RT-nPCR), and real-time RT-PCR (RT-qPCR). In addition, amplification and sequencing of the 5'UTR region were performed for phylogenetic purposes. Virus neutralization tests against the BVDV-1a, BVDV-1b, and BVDV-2b subtypes were conducted on 60 fetal fluids of the selected cases. Furthermore, the frequency and severity of histopathological lesions were evaluated in BVDV-positive cases. This study demonstrated that RT-nPCR and RT-qPCR were more suitable than AgELISA for BVDV detection in fetal specimens. However, the agreement between the two RT-PCR methods was moderate. The BVDV-1b subtype was more frequently detected than the BVDV-1a and BVDV-2b subtypes. Neutralizing antibodies to any of the three subtypes evaluated were present in 94% of the fetal fluids. Microscopically, half of the BVDV-positive cases showed a mild non-suppurative inflammatory response. These results emphasize the need to consider different methods for a diagnostic approach of BVDV associated to reproductive losses.
EEA Balcarce
Fil: Spetter, Maximiliano Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Verna, Andrea Elizabeth . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Dorsch, Matías A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Pereyra, Susana Beatriz . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Odeón, Anselmo C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Saliki, Jeremiah T. University of Georgia. Athens Veterinary Diagnostic Laboratory; Estados Unidos
Fil: González Altamiranda, Erika A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fuente
Brazilian journal of microbiology (2020)
Materia
Aborto
Diagnóstico
Filogenia
Patología
Abortion
Diagnosis
Phylogeny
Pathology
BVDV
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7354
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oai_identifier_str oai:localhost:20.500.12123/7354
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network_name_str INTA Digital (INTA)
spelling Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year periodSpetter, Lucas MaximilianoLouge Uriarte, Enrique LeopoldoArmendano, Joaquín IgnacioMorrell, Eleonora LidiaCanton, German JoseVerna, Andrea ElizabethDorsch, MatíasPereyra, Susana BeatrizOdeon, Anselmo CarlosSaliki, JeremiahGonzález Altamiranda, ErikaAbortoDiagnósticoFilogeniaPatologíaAbortionDiagnosisPhylogenyPathologyBVDVDetection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. Additionally, genetic, serological, and pathological analyses were conducted in order to characterize BVDV strains of fetal origin. Samples from 95 selected cases from 1997 to 2018 were analyzed by antigen-capture ELISA (AgELISA), nested RT-PCR (RT-nPCR), and real-time RT-PCR (RT-qPCR). In addition, amplification and sequencing of the 5'UTR region were performed for phylogenetic purposes. Virus neutralization tests against the BVDV-1a, BVDV-1b, and BVDV-2b subtypes were conducted on 60 fetal fluids of the selected cases. Furthermore, the frequency and severity of histopathological lesions were evaluated in BVDV-positive cases. This study demonstrated that RT-nPCR and RT-qPCR were more suitable than AgELISA for BVDV detection in fetal specimens. However, the agreement between the two RT-PCR methods was moderate. The BVDV-1b subtype was more frequently detected than the BVDV-1a and BVDV-2b subtypes. Neutralizing antibodies to any of the three subtypes evaluated were present in 94% of the fetal fluids. Microscopically, half of the BVDV-positive cases showed a mild non-suppurative inflammatory response. These results emphasize the need to consider different methods for a diagnostic approach of BVDV associated to reproductive losses.EEA BalcarceFil: Spetter, Maximiliano Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Verna, Andrea Elizabeth . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.Fil: Dorsch, Matías A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Pereyra, Susana Beatriz . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Odeón, Anselmo C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Saliki, Jeremiah T. University of Georgia. Athens Veterinary Diagnostic Laboratory; Estados UnidosFil: González Altamiranda, Erika A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaSpringer2020-06-03T17:41:33Z2020-06-03T17:41:33Z2020-05-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7354https://link.springer.com/article/10.1007/s42770-020-00296-z1517-8382https://doi.org/10.1007/s42770-020-00296-zBrazilian journal of microbiology (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2026-01-08T10:37:53Zoai:localhost:20.500.12123/7354instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:37:53.484INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
title Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
spellingShingle Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
Spetter, Lucas Maximiliano
Aborto
Diagnóstico
Filogenia
Patología
Abortion
Diagnosis
Phylogeny
Pathology
BVDV
title_short Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
title_full Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
title_fullStr Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
title_full_unstemmed Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
title_sort Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
dc.creator.none.fl_str_mv Spetter, Lucas Maximiliano
Louge Uriarte, Enrique Leopoldo
Armendano, Joaquín Ignacio
Morrell, Eleonora Lidia
Canton, German Jose
Verna, Andrea Elizabeth
Dorsch, Matías
Pereyra, Susana Beatriz
Odeon, Anselmo Carlos
Saliki, Jeremiah
González Altamiranda, Erika
author Spetter, Lucas Maximiliano
author_facet Spetter, Lucas Maximiliano
Louge Uriarte, Enrique Leopoldo
Armendano, Joaquín Ignacio
Morrell, Eleonora Lidia
Canton, German Jose
Verna, Andrea Elizabeth
Dorsch, Matías
Pereyra, Susana Beatriz
Odeon, Anselmo Carlos
Saliki, Jeremiah
González Altamiranda, Erika
author_role author
author2 Louge Uriarte, Enrique Leopoldo
Armendano, Joaquín Ignacio
Morrell, Eleonora Lidia
Canton, German Jose
Verna, Andrea Elizabeth
Dorsch, Matías
Pereyra, Susana Beatriz
Odeon, Anselmo Carlos
Saliki, Jeremiah
González Altamiranda, Erika
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aborto
Diagnóstico
Filogenia
Patología
Abortion
Diagnosis
Phylogeny
Pathology
BVDV
topic Aborto
Diagnóstico
Filogenia
Patología
Abortion
Diagnosis
Phylogeny
Pathology
BVDV
dc.description.none.fl_txt_mv Detection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. Additionally, genetic, serological, and pathological analyses were conducted in order to characterize BVDV strains of fetal origin. Samples from 95 selected cases from 1997 to 2018 were analyzed by antigen-capture ELISA (AgELISA), nested RT-PCR (RT-nPCR), and real-time RT-PCR (RT-qPCR). In addition, amplification and sequencing of the 5'UTR region were performed for phylogenetic purposes. Virus neutralization tests against the BVDV-1a, BVDV-1b, and BVDV-2b subtypes were conducted on 60 fetal fluids of the selected cases. Furthermore, the frequency and severity of histopathological lesions were evaluated in BVDV-positive cases. This study demonstrated that RT-nPCR and RT-qPCR were more suitable than AgELISA for BVDV detection in fetal specimens. However, the agreement between the two RT-PCR methods was moderate. The BVDV-1b subtype was more frequently detected than the BVDV-1a and BVDV-2b subtypes. Neutralizing antibodies to any of the three subtypes evaluated were present in 94% of the fetal fluids. Microscopically, half of the BVDV-positive cases showed a mild non-suppurative inflammatory response. These results emphasize the need to consider different methods for a diagnostic approach of BVDV associated to reproductive losses.
EEA Balcarce
Fil: Spetter, Maximiliano Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Verna, Andrea Elizabeth . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Dorsch, Matías A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Pereyra, Susana Beatriz . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Odeón, Anselmo C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Saliki, Jeremiah T. University of Georgia. Athens Veterinary Diagnostic Laboratory; Estados Unidos
Fil: González Altamiranda, Erika A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
description Detection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. Additionally, genetic, serological, and pathological analyses were conducted in order to characterize BVDV strains of fetal origin. Samples from 95 selected cases from 1997 to 2018 were analyzed by antigen-capture ELISA (AgELISA), nested RT-PCR (RT-nPCR), and real-time RT-PCR (RT-qPCR). In addition, amplification and sequencing of the 5'UTR region were performed for phylogenetic purposes. Virus neutralization tests against the BVDV-1a, BVDV-1b, and BVDV-2b subtypes were conducted on 60 fetal fluids of the selected cases. Furthermore, the frequency and severity of histopathological lesions were evaluated in BVDV-positive cases. This study demonstrated that RT-nPCR and RT-qPCR were more suitable than AgELISA for BVDV detection in fetal specimens. However, the agreement between the two RT-PCR methods was moderate. The BVDV-1b subtype was more frequently detected than the BVDV-1a and BVDV-2b subtypes. Neutralizing antibodies to any of the three subtypes evaluated were present in 94% of the fetal fluids. Microscopically, half of the BVDV-positive cases showed a mild non-suppurative inflammatory response. These results emphasize the need to consider different methods for a diagnostic approach of BVDV associated to reproductive losses.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-03T17:41:33Z
2020-06-03T17:41:33Z
2020-05-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7354
https://link.springer.com/article/10.1007/s42770-020-00296-z
1517-8382
https://doi.org/10.1007/s42770-020-00296-z
url http://hdl.handle.net/20.500.12123/7354
https://link.springer.com/article/10.1007/s42770-020-00296-z
https://doi.org/10.1007/s42770-020-00296-z
identifier_str_mv 1517-8382
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Brazilian journal of microbiology (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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