Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR

Autores
Cheuquepan Valenzuel, Felipe Andres; Recavarren, Mariana; Quintana, Silvina; Canton, German Jose; Odeon, Anselmo Carlos; Marin, Maia Solange; Morrell, Eleonora Lidia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a disease with major economic impact on livestock industry. The objective of this work was to determine the presence of Leptospira spp. DNA by qPCR in bovine fetuses with presumptive diagnosis of leptospirosis as the cause of abortion. Leptospira spp. DNA was detected by qPCR in 11 out of 34 fetuses. These specimens (10/11) had histopathological findings in hepatic and/or renal tissues compatible with leptospirosis. qPCR detection rate (32.4 %) was higher compared with direct immuno-fluorescence antibody test (DFAT) (11.8 %). The concordance coefficient between both techniques was 0.44. qPCR is a rapid and sensitive technique for the diagnosis of leptospirosis and improved the detection rate in fetal tissues compared with DFAT. Implementation of molecular techniques may increase the accurate detection of leptospirosis as a cause of bovine abortion allowing the application of rapid therapeutic and prophylactics measures in order to reduce the impact of this zoonotic disease.
EEA Balcarce
Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce). Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Recavarren, Mariana. Instituto de Análisis Fares Taie; Argentina
Fil: Quintana, Silvina. Instituto de Análisis Fares Taie; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Marin, Maia Solange. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce). Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fuente
Comparative Immunology, Microbiology and Infectious Diseases 73 : 101555 (2020)
Materia
Bovina
Aborto
Feto
Diagnóstico
PCR
Leptospira
Enfermedades de los Animales
Bovinae
Abortion
Foetus
Diagnosis
Animal Diseases
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8041
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8041
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network_name_str INTA Digital (INTA)
spelling Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCRCheuquepan Valenzuel, Felipe AndresRecavarren, MarianaQuintana, SilvinaCanton, German JoseOdeon, Anselmo CarlosMarin, Maia SolangeMorrell, Eleonora LidiaBovinaAbortoFetoDiagnósticoPCRLeptospiraEnfermedades de los AnimalesBovinaeAbortionFoetusDiagnosisAnimal DiseasesLeptospirosis is a disease with major economic impact on livestock industry. The objective of this work was to determine the presence of Leptospira spp. DNA by qPCR in bovine fetuses with presumptive diagnosis of leptospirosis as the cause of abortion. Leptospira spp. DNA was detected by qPCR in 11 out of 34 fetuses. These specimens (10/11) had histopathological findings in hepatic and/or renal tissues compatible with leptospirosis. qPCR detection rate (32.4 %) was higher compared with direct immuno-fluorescence antibody test (DFAT) (11.8 %). The concordance coefficient between both techniques was 0.44. qPCR is a rapid and sensitive technique for the diagnosis of leptospirosis and improved the detection rate in fetal tissues compared with DFAT. Implementation of molecular techniques may increase the accurate detection of leptospirosis as a cause of bovine abortion allowing the application of rapid therapeutic and prophylactics measures in order to reduce the impact of this zoonotic disease.EEA BalcarceFil: Cheuquepán Valenzuela, Felipe Andrés. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce). Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Recavarren, Mariana. Instituto de Análisis Fares Taie; ArgentinaFil: Quintana, Silvina. Instituto de Análisis Fares Taie; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Marin, Maia Solange. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce). Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Elsevier2020-10-13T18:46:03Z2020-10-13T18:46:03Z2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8041https://www.sciencedirect.com/science/article/abs/pii/S01479571203014420147-9571https://doi.org/10.1016/j.cimid.2020.101555Comparative Immunology, Microbiology and Infectious Diseases 73 : 101555 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:38Zoai:localhost:20.500.12123/8041instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:39.064INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
title Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
spellingShingle Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
Cheuquepan Valenzuel, Felipe Andres
Bovina
Aborto
Feto
Diagnóstico
PCR
Leptospira
Enfermedades de los Animales
Bovinae
Abortion
Foetus
Diagnosis
Animal Diseases
title_short Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
title_full Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
title_fullStr Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
title_full_unstemmed Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
title_sort Improvement of Leptospira spp. diagnosis in aborted bovine fetuses by qPCR
dc.creator.none.fl_str_mv Cheuquepan Valenzuel, Felipe Andres
Recavarren, Mariana
Quintana, Silvina
Canton, German Jose
Odeon, Anselmo Carlos
Marin, Maia Solange
Morrell, Eleonora Lidia
author Cheuquepan Valenzuel, Felipe Andres
author_facet Cheuquepan Valenzuel, Felipe Andres
Recavarren, Mariana
Quintana, Silvina
Canton, German Jose
Odeon, Anselmo Carlos
Marin, Maia Solange
Morrell, Eleonora Lidia
author_role author
author2 Recavarren, Mariana
Quintana, Silvina
Canton, German Jose
Odeon, Anselmo Carlos
Marin, Maia Solange
Morrell, Eleonora Lidia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bovina
Aborto
Feto
Diagnóstico
PCR
Leptospira
Enfermedades de los Animales
Bovinae
Abortion
Foetus
Diagnosis
Animal Diseases
topic Bovina
Aborto
Feto
Diagnóstico
PCR
Leptospira
Enfermedades de los Animales
Bovinae
Abortion
Foetus
Diagnosis
Animal Diseases
dc.description.none.fl_txt_mv Leptospirosis is a disease with major economic impact on livestock industry. The objective of this work was to determine the presence of Leptospira spp. DNA by qPCR in bovine fetuses with presumptive diagnosis of leptospirosis as the cause of abortion. Leptospira spp. DNA was detected by qPCR in 11 out of 34 fetuses. These specimens (10/11) had histopathological findings in hepatic and/or renal tissues compatible with leptospirosis. qPCR detection rate (32.4 %) was higher compared with direct immuno-fluorescence antibody test (DFAT) (11.8 %). The concordance coefficient between both techniques was 0.44. qPCR is a rapid and sensitive technique for the diagnosis of leptospirosis and improved the detection rate in fetal tissues compared with DFAT. Implementation of molecular techniques may increase the accurate detection of leptospirosis as a cause of bovine abortion allowing the application of rapid therapeutic and prophylactics measures in order to reduce the impact of this zoonotic disease.
EEA Balcarce
Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce). Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Recavarren, Mariana. Instituto de Análisis Fares Taie; Argentina
Fil: Quintana, Silvina. Instituto de Análisis Fares Taie; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Marin, Maia Solange. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce). Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
description Leptospirosis is a disease with major economic impact on livestock industry. The objective of this work was to determine the presence of Leptospira spp. DNA by qPCR in bovine fetuses with presumptive diagnosis of leptospirosis as the cause of abortion. Leptospira spp. DNA was detected by qPCR in 11 out of 34 fetuses. These specimens (10/11) had histopathological findings in hepatic and/or renal tissues compatible with leptospirosis. qPCR detection rate (32.4 %) was higher compared with direct immuno-fluorescence antibody test (DFAT) (11.8 %). The concordance coefficient between both techniques was 0.44. qPCR is a rapid and sensitive technique for the diagnosis of leptospirosis and improved the detection rate in fetal tissues compared with DFAT. Implementation of molecular techniques may increase the accurate detection of leptospirosis as a cause of bovine abortion allowing the application of rapid therapeutic and prophylactics measures in order to reduce the impact of this zoonotic disease.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-13T18:46:03Z
2020-10-13T18:46:03Z
2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8041
https://www.sciencedirect.com/science/article/abs/pii/S0147957120301442
0147-9571
https://doi.org/10.1016/j.cimid.2020.101555
url http://hdl.handle.net/20.500.12123/8041
https://www.sciencedirect.com/science/article/abs/pii/S0147957120301442
https://doi.org/10.1016/j.cimid.2020.101555
identifier_str_mv 0147-9571
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Comparative Immunology, Microbiology and Infectious Diseases 73 : 101555 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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