Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities

Autores
Agra, Marcelo Nicolás; Conte, Claudia Alejandra; Corva, Pablo Marcelo; Cladera, Jorge Luis; Lanzavecchia, Silvia Beatriz; Palacio, María Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The European honeybee, Apis mellifera L. (Hymenoptera: Apidae), is considered as a main contributor to pollination of important crops and to honey production. Originally, beekeeping in Argentina was performed in an extended area covering the north and central region of the country and involving A. mellifera of European origin. Later, honeybees of African origin entered South America through Brazil and hybridized with European genetic resources, giving rise to Africanized populations that are characterized by a more aggressive behavior among other unfavorable traits. In this study, a genetic characterization of 396 honeybee colonies from the most important apicultural region of Argentina is presented in order to provide an updated description of population structure and genetic diversity of commercial and feral colonies. Diversity was analyzed using mitochondrial (COI‐COII region) and nuclear (eight microsatellites) markers. Three European (M4, C1, C2J) and three African (A1, A4, A30) haplotypes were detected. European haplotypes were mostly found in commercial apiaries, whereas African haplotypes were detected at high frequencies in feral colonies. Microsatellite data were analyzed to estimate population genetic variability at the province level and to evaluate genetic admixture. A high level of hybridization between Africanized and European honeybees was detected with a significant latitudinal cline from north to south. Extensive population admixture resulted in the definition of four clusters that included both feral and commercial colonies and that are explained not only by geographical distribution and degree of Africanization but also by human influence through beekeeping activities.
EEA Balcarce
Fil: Agra, Marcelo Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Corva, Pablo M. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fuente
Entomologia Experimentalis et Applicata 166 (9) : 724–738 (September 2018)
Materia
Apidae
Apis Mellifera
PCR
RFLP
Microsatélites
Estructura de la Población
Hymenoptera
Microsatellites
Population Structure
Honey Bees
Abeja Melífera
Abejas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3721
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3721
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activitiesAgra, Marcelo NicolásConte, Claudia AlejandraCorva, Pablo MarceloCladera, Jorge LuisLanzavecchia, Silvia BeatrizPalacio, María AlejandraApidaeApis MelliferaPCRRFLPMicrosatélitesEstructura de la PoblaciónHymenopteraMicrosatellitesPopulation StructureHoney BeesAbeja MelíferaAbejasThe European honeybee, Apis mellifera L. (Hymenoptera: Apidae), is considered as a main contributor to pollination of important crops and to honey production. Originally, beekeeping in Argentina was performed in an extended area covering the north and central region of the country and involving A. mellifera of European origin. Later, honeybees of African origin entered South America through Brazil and hybridized with European genetic resources, giving rise to Africanized populations that are characterized by a more aggressive behavior among other unfavorable traits. In this study, a genetic characterization of 396 honeybee colonies from the most important apicultural region of Argentina is presented in order to provide an updated description of population structure and genetic diversity of commercial and feral colonies. Diversity was analyzed using mitochondrial (COI‐COII region) and nuclear (eight microsatellites) markers. Three European (M4, C1, C2J) and three African (A1, A4, A30) haplotypes were detected. European haplotypes were mostly found in commercial apiaries, whereas African haplotypes were detected at high frequencies in feral colonies. Microsatellite data were analyzed to estimate population genetic variability at the province level and to evaluate genetic admixture. A high level of hybridization between Africanized and European honeybees was detected with a significant latitudinal cline from north to south. Extensive population admixture resulted in the definition of four clusters that included both feral and commercial colonies and that are explained not only by geographical distribution and degree of Africanization but also by human influence through beekeeping activities.EEA BalcarceFil: Agra, Marcelo Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Corva, Pablo M. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaWiley Online Library2018-10-26T14:44:07Z2018-10-26T14:44:07Z2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/full/10.1111/eea.12719http://hdl.handle.net/20.500.12123/37211570-7458https://doi.org/10.1111/eea.12719Entomologia Experimentalis et Applicata 166 (9) : 724–738 (September 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:43Zoai:localhost:20.500.12123/3721instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:43.531INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities
title Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities
spellingShingle Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities
Agra, Marcelo Nicolás
Apidae
Apis Mellifera
PCR
RFLP
Microsatélites
Estructura de la Población
Hymenoptera
Microsatellites
Population Structure
Honey Bees
Abeja Melífera
Abejas
title_short Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities
title_full Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities
title_fullStr Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities
title_full_unstemmed Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities
title_sort Molecular characterization of Apis mellifera colonies from Argentina: genotypic admixture associated with ecoclimatic regions and apicultural activities
dc.creator.none.fl_str_mv Agra, Marcelo Nicolás
Conte, Claudia Alejandra
Corva, Pablo Marcelo
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
Palacio, María Alejandra
author Agra, Marcelo Nicolás
author_facet Agra, Marcelo Nicolás
Conte, Claudia Alejandra
Corva, Pablo Marcelo
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
Palacio, María Alejandra
author_role author
author2 Conte, Claudia Alejandra
Corva, Pablo Marcelo
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
Palacio, María Alejandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Apidae
Apis Mellifera
PCR
RFLP
Microsatélites
Estructura de la Población
Hymenoptera
Microsatellites
Population Structure
Honey Bees
Abeja Melífera
Abejas
topic Apidae
Apis Mellifera
PCR
RFLP
Microsatélites
Estructura de la Población
Hymenoptera
Microsatellites
Population Structure
Honey Bees
Abeja Melífera
Abejas
dc.description.none.fl_txt_mv The European honeybee, Apis mellifera L. (Hymenoptera: Apidae), is considered as a main contributor to pollination of important crops and to honey production. Originally, beekeeping in Argentina was performed in an extended area covering the north and central region of the country and involving A. mellifera of European origin. Later, honeybees of African origin entered South America through Brazil and hybridized with European genetic resources, giving rise to Africanized populations that are characterized by a more aggressive behavior among other unfavorable traits. In this study, a genetic characterization of 396 honeybee colonies from the most important apicultural region of Argentina is presented in order to provide an updated description of population structure and genetic diversity of commercial and feral colonies. Diversity was analyzed using mitochondrial (COI‐COII region) and nuclear (eight microsatellites) markers. Three European (M4, C1, C2J) and three African (A1, A4, A30) haplotypes were detected. European haplotypes were mostly found in commercial apiaries, whereas African haplotypes were detected at high frequencies in feral colonies. Microsatellite data were analyzed to estimate population genetic variability at the province level and to evaluate genetic admixture. A high level of hybridization between Africanized and European honeybees was detected with a significant latitudinal cline from north to south. Extensive population admixture resulted in the definition of four clusters that included both feral and commercial colonies and that are explained not only by geographical distribution and degree of Africanization but also by human influence through beekeeping activities.
EEA Balcarce
Fil: Agra, Marcelo Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Corva, Pablo M. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
description The European honeybee, Apis mellifera L. (Hymenoptera: Apidae), is considered as a main contributor to pollination of important crops and to honey production. Originally, beekeeping in Argentina was performed in an extended area covering the north and central region of the country and involving A. mellifera of European origin. Later, honeybees of African origin entered South America through Brazil and hybridized with European genetic resources, giving rise to Africanized populations that are characterized by a more aggressive behavior among other unfavorable traits. In this study, a genetic characterization of 396 honeybee colonies from the most important apicultural region of Argentina is presented in order to provide an updated description of population structure and genetic diversity of commercial and feral colonies. Diversity was analyzed using mitochondrial (COI‐COII region) and nuclear (eight microsatellites) markers. Three European (M4, C1, C2J) and three African (A1, A4, A30) haplotypes were detected. European haplotypes were mostly found in commercial apiaries, whereas African haplotypes were detected at high frequencies in feral colonies. Microsatellite data were analyzed to estimate population genetic variability at the province level and to evaluate genetic admixture. A high level of hybridization between Africanized and European honeybees was detected with a significant latitudinal cline from north to south. Extensive population admixture resulted in the definition of four clusters that included both feral and commercial colonies and that are explained not only by geographical distribution and degree of Africanization but also by human influence through beekeeping activities.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-26T14:44:07Z
2018-10-26T14:44:07Z
2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/full/10.1111/eea.12719
http://hdl.handle.net/20.500.12123/3721
1570-7458
https://doi.org/10.1111/eea.12719
url https://onlinelibrary.wiley.com/doi/full/10.1111/eea.12719
http://hdl.handle.net/20.500.12123/3721
https://doi.org/10.1111/eea.12719
identifier_str_mv 1570-7458
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley Online Library
publisher.none.fl_str_mv Wiley Online Library
dc.source.none.fl_str_mv Entomologia Experimentalis et Applicata 166 (9) : 724–738 (September 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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