Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina

Autores
Scannapieco, Alejandra Carla; Mannino, Constanza; Soto, Gabriela Cinthia; Palacio, Marí­a Alejandra; Cladera, Jorge Luis; Lanzavecchia, Silvia Beatriz
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hygienic behavior is an economically beneficial, heritable trait, which has evolved to limit the impact of honeybee pathogens. Selecting and breeding colonies with high levels of hygienic behavior has become a feasible and environmentally friendly strategy to control brood diseases in honeybee colonies worldwide. The identification of genes involved in the expression of this character may not only unravel molecular and biochemical pathways underlying hygienic behavior, but also serve as a practical approach to select disease resistance biomarkers useful for honeybee breeding programs. In the present work, we evaluated, at genetic level, Apis mellifera stocks selected for hygienic behavior, widely used for commercial apiculture in Argentina. We analyzed the expression profiles of five genes previously identified as candidates associated with hygienic behavior both in QTL and global gene expression studies in honeybees, more precisely, involved in perception and processing of olfactory information. We validated the differential expression of these genes as potentially responsible for behavioral differences in our selected stocks. Our results indicate that four of them (octopamine receptor, smell-impaired, odorant-binding protein 3, and odorant-binding protein 4) were differentially expressed between hygienic and non-hygienic bees within our highly hygienic colonies. The present findings improve our understanding of the molecular mechanisms underlying the differentiation of middle-age worker bees in their genetic propensity to perform hygienic behavior. This progress towards the genetic characterization of highly hygienic colonies that are commercially used in Argentine apiculture lays the groundwork for future development of targets for marker-assisted selection of disease-resistant honeybee stocks.
Inst. de Genética "Ewald A. Favret"- IGEAF
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mannino, Constanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
Insectes sociaux 64 (4) : 485–494. (November 2017)
Materia
Abeja Melífera
Apis Mellifera
Comportamiento
Behaviour
Genética
Honey Bees
Genetics
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1977
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1977
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from ArgentinaScannapieco, Alejandra CarlaMannino, ConstanzaSoto, Gabriela CinthiaPalacio, Marí­a AlejandraCladera, Jorge LuisLanzavecchia, Silvia BeatrizAbeja MelíferaApis MelliferaComportamientoBehaviourGenéticaHoney BeesGeneticsHygienic behavior is an economically beneficial, heritable trait, which has evolved to limit the impact of honeybee pathogens. Selecting and breeding colonies with high levels of hygienic behavior has become a feasible and environmentally friendly strategy to control brood diseases in honeybee colonies worldwide. The identification of genes involved in the expression of this character may not only unravel molecular and biochemical pathways underlying hygienic behavior, but also serve as a practical approach to select disease resistance biomarkers useful for honeybee breeding programs. In the present work, we evaluated, at genetic level, Apis mellifera stocks selected for hygienic behavior, widely used for commercial apiculture in Argentina. We analyzed the expression profiles of five genes previously identified as candidates associated with hygienic behavior both in QTL and global gene expression studies in honeybees, more precisely, involved in perception and processing of olfactory information. We validated the differential expression of these genes as potentially responsible for behavioral differences in our selected stocks. Our results indicate that four of them (octopamine receptor, smell-impaired, odorant-binding protein 3, and odorant-binding protein 4) were differentially expressed between hygienic and non-hygienic bees within our highly hygienic colonies. The present findings improve our understanding of the molecular mechanisms underlying the differentiation of middle-age worker bees in their genetic propensity to perform hygienic behavior. This progress towards the genetic characterization of highly hygienic colonies that are commercially used in Argentine apiculture lays the groundwork for future development of targets for marker-assisted selection of disease-resistant honeybee stocks.Inst. de Genética "Ewald A. Favret"- IGEAFFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mannino, Constanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina2018-03-07T13:26:44Z2018-03-07T13:26:44Z2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1977https://link.springer.com/article/10.1007/s00040-017-0567-60020-1812 (Print)1420-9098 (Online)https://doi.org/10.1007/s00040-017-0567-6Insectes sociaux 64 (4) : 485–494. (November 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-10-16T09:29:06Zoai:localhost:20.500.12123/1977instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:06.778INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
title Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
spellingShingle Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
Scannapieco, Alejandra Carla
Abeja Melífera
Apis Mellifera
Comportamiento
Behaviour
Genética
Honey Bees
Genetics
title_short Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
title_full Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
title_fullStr Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
title_full_unstemmed Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
title_sort Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
dc.creator.none.fl_str_mv Scannapieco, Alejandra Carla
Mannino, Constanza
Soto, Gabriela Cinthia
Palacio, Marí­a Alejandra
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
author Scannapieco, Alejandra Carla
author_facet Scannapieco, Alejandra Carla
Mannino, Constanza
Soto, Gabriela Cinthia
Palacio, Marí­a Alejandra
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
author_role author
author2 Mannino, Constanza
Soto, Gabriela Cinthia
Palacio, Marí­a Alejandra
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Abeja Melífera
Apis Mellifera
Comportamiento
Behaviour
Genética
Honey Bees
Genetics
topic Abeja Melífera
Apis Mellifera
Comportamiento
Behaviour
Genética
Honey Bees
Genetics
dc.description.none.fl_txt_mv Hygienic behavior is an economically beneficial, heritable trait, which has evolved to limit the impact of honeybee pathogens. Selecting and breeding colonies with high levels of hygienic behavior has become a feasible and environmentally friendly strategy to control brood diseases in honeybee colonies worldwide. The identification of genes involved in the expression of this character may not only unravel molecular and biochemical pathways underlying hygienic behavior, but also serve as a practical approach to select disease resistance biomarkers useful for honeybee breeding programs. In the present work, we evaluated, at genetic level, Apis mellifera stocks selected for hygienic behavior, widely used for commercial apiculture in Argentina. We analyzed the expression profiles of five genes previously identified as candidates associated with hygienic behavior both in QTL and global gene expression studies in honeybees, more precisely, involved in perception and processing of olfactory information. We validated the differential expression of these genes as potentially responsible for behavioral differences in our selected stocks. Our results indicate that four of them (octopamine receptor, smell-impaired, odorant-binding protein 3, and odorant-binding protein 4) were differentially expressed between hygienic and non-hygienic bees within our highly hygienic colonies. The present findings improve our understanding of the molecular mechanisms underlying the differentiation of middle-age worker bees in their genetic propensity to perform hygienic behavior. This progress towards the genetic characterization of highly hygienic colonies that are commercially used in Argentine apiculture lays the groundwork for future development of targets for marker-assisted selection of disease-resistant honeybee stocks.
Inst. de Genética "Ewald A. Favret"- IGEAF
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mannino, Constanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description Hygienic behavior is an economically beneficial, heritable trait, which has evolved to limit the impact of honeybee pathogens. Selecting and breeding colonies with high levels of hygienic behavior has become a feasible and environmentally friendly strategy to control brood diseases in honeybee colonies worldwide. The identification of genes involved in the expression of this character may not only unravel molecular and biochemical pathways underlying hygienic behavior, but also serve as a practical approach to select disease resistance biomarkers useful for honeybee breeding programs. In the present work, we evaluated, at genetic level, Apis mellifera stocks selected for hygienic behavior, widely used for commercial apiculture in Argentina. We analyzed the expression profiles of five genes previously identified as candidates associated with hygienic behavior both in QTL and global gene expression studies in honeybees, more precisely, involved in perception and processing of olfactory information. We validated the differential expression of these genes as potentially responsible for behavioral differences in our selected stocks. Our results indicate that four of them (octopamine receptor, smell-impaired, odorant-binding protein 3, and odorant-binding protein 4) were differentially expressed between hygienic and non-hygienic bees within our highly hygienic colonies. The present findings improve our understanding of the molecular mechanisms underlying the differentiation of middle-age worker bees in their genetic propensity to perform hygienic behavior. This progress towards the genetic characterization of highly hygienic colonies that are commercially used in Argentine apiculture lays the groundwork for future development of targets for marker-assisted selection of disease-resistant honeybee stocks.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
2018-03-07T13:26:44Z
2018-03-07T13:26:44Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1977
https://link.springer.com/article/10.1007/s00040-017-0567-6
0020-1812 (Print)
1420-9098 (Online)
https://doi.org/10.1007/s00040-017-0567-6
url http://hdl.handle.net/20.500.12123/1977
https://link.springer.com/article/10.1007/s00040-017-0567-6
https://doi.org/10.1007/s00040-017-0567-6
identifier_str_mv 0020-1812 (Print)
1420-9098 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.source.none.fl_str_mv Insectes sociaux 64 (4) : 485–494. (November 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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