Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera

Autores
Liendo, María Clara; Muntaabski, Irina; Russo, Romina Maria; Lanzavecchia, Silvia Beatriz; Segura, Diego Fernando; Palacio, María Alejandra; Cladera, Jorge Luis; Fernández, Patricia Carina; Scannapieco, Alejandra Carla
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Varroa destructor Anderson & Trueman (Acari: Varroidae) is one of the major contributors to the significant losses of western honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies worldwide. Hygienic behavior, in which individual workers detect, uncap, and remove unhealthy brood, is a type of social immunity that reduces pathogen and parasite loads in the colony. Previous evidence suggests that hygienic worker bees identify diseased brood through olfactory cues. The aims of the present work were (1) to study the hygienic behavior of worker bees toward V. destructor-infested cells at different stages of brood development, (2) to explore changes in brood volatile profiles associated with the progression of mite infestation, and (3) to analyze the role of specific volatile compounds in triggering the hygienic behavior. Results showed that the removal rate of infested brood changed along its development and the progression of mite reproduction. Two compounds, ethyl hexanoate and α-pinene, were present in volatile collections from mite-infested pupae but absent from uninfested pupae. Field bioassays showed that these volatiles are relevant to elicit the hygienic behavior. A third compound, β-ocimene, was present in infested and uninfested brood but its abundance pattern varied according to the infestation status throughout brood development. Specifically, for uninfested brood, the abundance of β-ocimene showed a reduction in black-eyed pupae, whereas for infested brood, its abundance decreased drastically in light-pink-eyed pupae and remained constant in black-eyed pupae. Our results revealed that olfactory signals associated with V. destructor infestation change as the reproductive cycle of V. destructor progresses inside the cell. These changes can be mimicked to some extent by adding specific volatile compounds to the cell to induce hygienic removal. These findings shed light on the chemical basis of hygienic behavior against V. destructor and could facilitate the development of improved hygienic selection tools to breed mite-resistant honey bee colonies.
EEA Balcarce
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Russo, Romina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Palacio, María Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Fernández, Patricia Carina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina.
Fil: Fernández, Patricia Carina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fuente
Entomologia Experimentalis et Applicata 169 : 563–574 (June 2021)
Materia
Abeja Melífera
Comportamiento Animal
Compuesto Volátil
Varroa Destructor
Apis Melífera
Honey Bees
Animal Behaviour
Volatile Compounds
Acarina
Hymenoptera
Apidae
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis melliferaLiendo, María ClaraMuntaabski, IrinaRusso, Romina MariaLanzavecchia, Silvia BeatrizSegura, Diego FernandoPalacio, María AlejandraCladera, Jorge LuisFernández, Patricia CarinaScannapieco, Alejandra CarlaAbeja MelíferaComportamiento AnimalCompuesto VolátilVarroa DestructorApis MelíferaHoney BeesAnimal BehaviourVolatile CompoundsAcarinaHymenopteraApidaeVarroa destructor Anderson & Trueman (Acari: Varroidae) is one of the major contributors to the significant losses of western honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies worldwide. Hygienic behavior, in which individual workers detect, uncap, and remove unhealthy brood, is a type of social immunity that reduces pathogen and parasite loads in the colony. Previous evidence suggests that hygienic worker bees identify diseased brood through olfactory cues. The aims of the present work were (1) to study the hygienic behavior of worker bees toward V. destructor-infested cells at different stages of brood development, (2) to explore changes in brood volatile profiles associated with the progression of mite infestation, and (3) to analyze the role of specific volatile compounds in triggering the hygienic behavior. Results showed that the removal rate of infested brood changed along its development and the progression of mite reproduction. Two compounds, ethyl hexanoate and α-pinene, were present in volatile collections from mite-infested pupae but absent from uninfested pupae. Field bioassays showed that these volatiles are relevant to elicit the hygienic behavior. A third compound, β-ocimene, was present in infested and uninfested brood but its abundance pattern varied according to the infestation status throughout brood development. Specifically, for uninfested brood, the abundance of β-ocimene showed a reduction in black-eyed pupae, whereas for infested brood, its abundance decreased drastically in light-pink-eyed pupae and remained constant in black-eyed pupae. Our results revealed that olfactory signals associated with V. destructor infestation change as the reproductive cycle of V. destructor progresses inside the cell. These changes can be mimicked to some extent by adding specific volatile compounds to the cell to induce hygienic removal. These findings shed light on the chemical basis of hygienic behavior against V. destructor and could facilitate the development of improved hygienic selection tools to breed mite-resistant honey bee colonies.EEA BalcarceFil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Russo, Romina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Palacio, María Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Fernández, Patricia Carina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina.Fil: Fernández, Patricia Carina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Wiley2022-11-28T10:51:26Z2022-11-28T10:51:26Z2021-03-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13457https://onlinelibrary.wiley.com/doi/full/10.1111/eea.130481570-7458https://doi.org/10.1111/eea.13048Entomologia Experimentalis et Applicata 169 : 563–574 (June 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena.info:eu-repo/semantics/restrictedAccess2025-10-16T09:31:00Zoai:localhost:20.500.12123/13457instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:00.472INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
title Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
spellingShingle Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
Liendo, María Clara
Abeja Melífera
Comportamiento Animal
Compuesto Volátil
Varroa Destructor
Apis Melífera
Honey Bees
Animal Behaviour
Volatile Compounds
Acarina
Hymenoptera
Apidae
title_short Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
title_full Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
title_fullStr Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
title_full_unstemmed Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
title_sort Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
dc.creator.none.fl_str_mv Liendo, María Clara
Muntaabski, Irina
Russo, Romina Maria
Lanzavecchia, Silvia Beatriz
Segura, Diego Fernando
Palacio, María Alejandra
Cladera, Jorge Luis
Fernández, Patricia Carina
Scannapieco, Alejandra Carla
author Liendo, María Clara
author_facet Liendo, María Clara
Muntaabski, Irina
Russo, Romina Maria
Lanzavecchia, Silvia Beatriz
Segura, Diego Fernando
Palacio, María Alejandra
Cladera, Jorge Luis
Fernández, Patricia Carina
Scannapieco, Alejandra Carla
author_role author
author2 Muntaabski, Irina
Russo, Romina Maria
Lanzavecchia, Silvia Beatriz
Segura, Diego Fernando
Palacio, María Alejandra
Cladera, Jorge Luis
Fernández, Patricia Carina
Scannapieco, Alejandra Carla
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Abeja Melífera
Comportamiento Animal
Compuesto Volátil
Varroa Destructor
Apis Melífera
Honey Bees
Animal Behaviour
Volatile Compounds
Acarina
Hymenoptera
Apidae
topic Abeja Melífera
Comportamiento Animal
Compuesto Volátil
Varroa Destructor
Apis Melífera
Honey Bees
Animal Behaviour
Volatile Compounds
Acarina
Hymenoptera
Apidae
dc.description.none.fl_txt_mv Varroa destructor Anderson & Trueman (Acari: Varroidae) is one of the major contributors to the significant losses of western honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies worldwide. Hygienic behavior, in which individual workers detect, uncap, and remove unhealthy brood, is a type of social immunity that reduces pathogen and parasite loads in the colony. Previous evidence suggests that hygienic worker bees identify diseased brood through olfactory cues. The aims of the present work were (1) to study the hygienic behavior of worker bees toward V. destructor-infested cells at different stages of brood development, (2) to explore changes in brood volatile profiles associated with the progression of mite infestation, and (3) to analyze the role of specific volatile compounds in triggering the hygienic behavior. Results showed that the removal rate of infested brood changed along its development and the progression of mite reproduction. Two compounds, ethyl hexanoate and α-pinene, were present in volatile collections from mite-infested pupae but absent from uninfested pupae. Field bioassays showed that these volatiles are relevant to elicit the hygienic behavior. A third compound, β-ocimene, was present in infested and uninfested brood but its abundance pattern varied according to the infestation status throughout brood development. Specifically, for uninfested brood, the abundance of β-ocimene showed a reduction in black-eyed pupae, whereas for infested brood, its abundance decreased drastically in light-pink-eyed pupae and remained constant in black-eyed pupae. Our results revealed that olfactory signals associated with V. destructor infestation change as the reproductive cycle of V. destructor progresses inside the cell. These changes can be mimicked to some extent by adding specific volatile compounds to the cell to induce hygienic removal. These findings shed light on the chemical basis of hygienic behavior against V. destructor and could facilitate the development of improved hygienic selection tools to breed mite-resistant honey bee colonies.
EEA Balcarce
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Russo, Romina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Palacio, María Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Fernández, Patricia Carina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina.
Fil: Fernández, Patricia Carina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
description Varroa destructor Anderson & Trueman (Acari: Varroidae) is one of the major contributors to the significant losses of western honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies worldwide. Hygienic behavior, in which individual workers detect, uncap, and remove unhealthy brood, is a type of social immunity that reduces pathogen and parasite loads in the colony. Previous evidence suggests that hygienic worker bees identify diseased brood through olfactory cues. The aims of the present work were (1) to study the hygienic behavior of worker bees toward V. destructor-infested cells at different stages of brood development, (2) to explore changes in brood volatile profiles associated with the progression of mite infestation, and (3) to analyze the role of specific volatile compounds in triggering the hygienic behavior. Results showed that the removal rate of infested brood changed along its development and the progression of mite reproduction. Two compounds, ethyl hexanoate and α-pinene, were present in volatile collections from mite-infested pupae but absent from uninfested pupae. Field bioassays showed that these volatiles are relevant to elicit the hygienic behavior. A third compound, β-ocimene, was present in infested and uninfested brood but its abundance pattern varied according to the infestation status throughout brood development. Specifically, for uninfested brood, the abundance of β-ocimene showed a reduction in black-eyed pupae, whereas for infested brood, its abundance decreased drastically in light-pink-eyed pupae and remained constant in black-eyed pupae. Our results revealed that olfactory signals associated with V. destructor infestation change as the reproductive cycle of V. destructor progresses inside the cell. These changes can be mimicked to some extent by adding specific volatile compounds to the cell to induce hygienic removal. These findings shed light on the chemical basis of hygienic behavior against V. destructor and could facilitate the development of improved hygienic selection tools to breed mite-resistant honey bee colonies.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-16
2022-11-28T10:51:26Z
2022-11-28T10:51:26Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13457
https://onlinelibrary.wiley.com/doi/full/10.1111/eea.13048
1570-7458
https://doi.org/10.1111/eea.13048
url http://hdl.handle.net/20.500.12123/13457
https://onlinelibrary.wiley.com/doi/full/10.1111/eea.13048
https://doi.org/10.1111/eea.13048
identifier_str_mv 1570-7458
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Entomologia Experimentalis et Applicata 169 : 563–574 (June 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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