Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system

Autores
Canizo, Jésica Romina; Vazquez Echegaray, Camila; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante; Alberio, Ricardo; Alberio, Ramiro; Guberman, Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. RESULTS: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.
EEA Balcarce
Fil: Canizo, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Vazquez Echegaray, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klisch, Doris. University of Nottingham. School of Biosciences; Gran Bretaña
Fil: Aller Atucha, Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Paz, Dante. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales Departamento de Biodiversidad y Biología Experimental, Buenos Aires, Argentina. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires; Argentina
Fil: Alberio, Ricardo. INTA Estación Experimental Agropecuaria Balcarce, Buenos Aires; Argentina Universidad Nacional de Mar del Plata, Facultad de Ciencias Agrarias, Balcarce; Argentina
Fil: Alberio, Ramiro. University of Nottingham, School of Biosciences, Nottingham; Gran Bretaña
Fil: Guberman, Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica. Departamento de Fisiología y Biología Molecular y Celular; Argentina
Fuente
BMC Research Notes 11 : 509 (2018)
Materia
Biotecnología
Animales Transgénicos
Células
Fibroblastos
Vectores Genéticos
Biotechnology
Transgenic Animals
Cells
Fibroblasts
Genetic Vectors
Bovino
Porcino
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3086
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3086
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery systemCanizo, Jésica RominaVazquez Echegaray, CamilaKlisch, DorisAller Atucha, Juan FlorencioPaz, DanteAlberio, RicardoAlberio, RamiroGuberman, AlejandraBiotecnologíaAnimales TransgénicosCélulasFibroblastosVectores GenéticosBiotechnologyTransgenic AnimalsCellsFibroblastsGenetic VectorsBovinoPorcinoOBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. RESULTS: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.EEA BalcarceFil: Canizo, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Vazquez Echegaray, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Klisch, Doris. University of Nottingham. School of Biosciences; Gran BretañaFil: Aller Atucha, Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Paz, Dante. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales Departamento de Biodiversidad y Biología Experimental, Buenos Aires, Argentina. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires; ArgentinaFil: Alberio, Ricardo. INTA Estación Experimental Agropecuaria Balcarce, Buenos Aires; Argentina Universidad Nacional de Mar del Plata, Facultad de Ciencias Agrarias, Balcarce; ArgentinaFil: Alberio, Ramiro. University of Nottingham, School of Biosciences, Nottingham; Gran BretañaFil: Guberman, Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica. Departamento de Fisiología y Biología Molecular y Celular; ArgentinaBioMedCentral2018-08-15T15:37:34Z2018-08-15T15:37:34Z2018-07-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3627-8http://hdl.handle.net/20.500.12123/30861756-0500https://doi.org/10.1186/s13104-018-3627-8BMC Research Notes 11 : 509 (2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:22:29Zoai:localhost:20.500.12123/3086instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:22:29.722INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
spellingShingle Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
Canizo, Jésica Romina
Biotecnología
Animales Transgénicos
Células
Fibroblastos
Vectores Genéticos
Biotechnology
Transgenic Animals
Cells
Fibroblasts
Genetic Vectors
Bovino
Porcino
title_short Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title_full Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title_fullStr Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title_full_unstemmed Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title_sort Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
dc.creator.none.fl_str_mv Canizo, Jésica Romina
Vazquez Echegaray, Camila
Klisch, Doris
Aller Atucha, Juan Florencio
Paz, Dante
Alberio, Ricardo
Alberio, Ramiro
Guberman, Alejandra
author Canizo, Jésica Romina
author_facet Canizo, Jésica Romina
Vazquez Echegaray, Camila
Klisch, Doris
Aller Atucha, Juan Florencio
Paz, Dante
Alberio, Ricardo
Alberio, Ramiro
Guberman, Alejandra
author_role author
author2 Vazquez Echegaray, Camila
Klisch, Doris
Aller Atucha, Juan Florencio
Paz, Dante
Alberio, Ricardo
Alberio, Ramiro
Guberman, Alejandra
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biotecnología
Animales Transgénicos
Células
Fibroblastos
Vectores Genéticos
Biotechnology
Transgenic Animals
Cells
Fibroblasts
Genetic Vectors
Bovino
Porcino
topic Biotecnología
Animales Transgénicos
Células
Fibroblastos
Vectores Genéticos
Biotechnology
Transgenic Animals
Cells
Fibroblasts
Genetic Vectors
Bovino
Porcino
dc.description.none.fl_txt_mv OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. RESULTS: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.
EEA Balcarce
Fil: Canizo, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Vazquez Echegaray, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klisch, Doris. University of Nottingham. School of Biosciences; Gran Bretaña
Fil: Aller Atucha, Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Paz, Dante. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales Departamento de Biodiversidad y Biología Experimental, Buenos Aires, Argentina. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires; Argentina
Fil: Alberio, Ricardo. INTA Estación Experimental Agropecuaria Balcarce, Buenos Aires; Argentina Universidad Nacional de Mar del Plata, Facultad de Ciencias Agrarias, Balcarce; Argentina
Fil: Alberio, Ramiro. University of Nottingham, School of Biosciences, Nottingham; Gran Bretaña
Fil: Guberman, Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica. Departamento de Fisiología y Biología Molecular y Celular; Argentina
description OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. RESULTS: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-15T15:37:34Z
2018-08-15T15:37:34Z
2018-07-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3627-8
http://hdl.handle.net/20.500.12123/3086
1756-0500
https://doi.org/10.1186/s13104-018-3627-8
url https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3627-8
http://hdl.handle.net/20.500.12123/3086
https://doi.org/10.1186/s13104-018-3627-8
identifier_str_mv 1756-0500
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMedCentral
publisher.none.fl_str_mv BioMedCentral
dc.source.none.fl_str_mv BMC Research Notes 11 : 509 (2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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