Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system

Autores
Canizo, Jesica Romina; Vazquez Echegaray, Camila; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante Agustin; Alberio, Ricardo; Alberio, Ramiro; Guberman, Alejandra Sonia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. Results: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.
Fil: Canizo J. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vazquez Echegaray, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Klisch D. Science and Technology Facilities Council of Nottingham. Rutherford Appleton Laboratory; Reino Unido
Fil: Aller Atucha J. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Paz D. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Alberio RH. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Alberio R. Science and Technology Facilities Council of Nottingham. Rutherford Appleton Laboratory; Reino Unido
Fil: Guberman, Alejandra Sonia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
BOVINE AND PORCINE FIBROBLASTS
IPS-LIKE CELLS
LENTIVIRAL VECTORS
REPROGRAMMING
STEMCCA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97996
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/97996
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery systemCanizo, Jesica RominaVazquez Echegaray, CamilaKlisch, DorisAller Atucha, Juan FlorencioPaz, Dante AgustinAlberio, RicardoAlberio, RamiroGuberman, Alejandra SoniaBOVINE AND PORCINE FIBROBLASTSIPS-LIKE CELLSLENTIVIRAL VECTORSREPROGRAMMINGSTEMCCAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. Results: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.Fil: Canizo J. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Vazquez Echegaray, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Klisch D. Science and Technology Facilities Council of Nottingham. Rutherford Appleton Laboratory; Reino UnidoFil: Aller Atucha J. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Paz D. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Alberio RH. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Alberio R. Science and Technology Facilities Council of Nottingham. Rutherford Appleton Laboratory; Reino UnidoFil: Guberman, Alejandra Sonia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaBioMed Central Ltd.2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97996Canizo, Jesica Romina; Vazquez Echegaray, Camila; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante Agustin; et al.; Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system; BioMed Central Ltd.; BMC Research Notes; 11; 1; 7-2018; 1-81756-0500CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13104-018-3627-8info:eu-repo/semantics/altIdentifier/url/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3627-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:40:47Zoai:ri.conicet.gov.ar:11336/97996instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:40:47.699CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
spellingShingle Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
Canizo, Jesica Romina
BOVINE AND PORCINE FIBROBLASTS
IPS-LIKE CELLS
LENTIVIRAL VECTORS
REPROGRAMMING
STEMCCA
title_short Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title_full Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title_fullStr Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title_full_unstemmed Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
title_sort Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
dc.creator.none.fl_str_mv Canizo, Jesica Romina
Vazquez Echegaray, Camila
Klisch, Doris
Aller Atucha, Juan Florencio
Paz, Dante Agustin
Alberio, Ricardo
Alberio, Ramiro
Guberman, Alejandra Sonia
author Canizo, Jesica Romina
author_facet Canizo, Jesica Romina
Vazquez Echegaray, Camila
Klisch, Doris
Aller Atucha, Juan Florencio
Paz, Dante Agustin
Alberio, Ricardo
Alberio, Ramiro
Guberman, Alejandra Sonia
author_role author
author2 Vazquez Echegaray, Camila
Klisch, Doris
Aller Atucha, Juan Florencio
Paz, Dante Agustin
Alberio, Ricardo
Alberio, Ramiro
Guberman, Alejandra Sonia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE AND PORCINE FIBROBLASTS
IPS-LIKE CELLS
LENTIVIRAL VECTORS
REPROGRAMMING
STEMCCA
topic BOVINE AND PORCINE FIBROBLASTS
IPS-LIKE CELLS
LENTIVIRAL VECTORS
REPROGRAMMING
STEMCCA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. Results: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.
Fil: Canizo J. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vazquez Echegaray, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Klisch D. Science and Technology Facilities Council of Nottingham. Rutherford Appleton Laboratory; Reino Unido
Fil: Aller Atucha J. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Paz D. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Alberio RH. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Alberio R. Science and Technology Facilities Council of Nottingham. Rutherford Appleton Laboratory; Reino Unido
Fil: Guberman, Alejandra Sonia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. Results: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97996
Canizo, Jesica Romina; Vazquez Echegaray, Camila; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante Agustin; et al.; Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system; BioMed Central Ltd.; BMC Research Notes; 11; 1; 7-2018; 1-8
1756-0500
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97996
identifier_str_mv Canizo, Jesica Romina; Vazquez Echegaray, Camila; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante Agustin; et al.; Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system; BioMed Central Ltd.; BMC Research Notes; 11; 1; 7-2018; 1-8
1756-0500
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s13104-018-3627-8
info:eu-repo/semantics/altIdentifier/url/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3627-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central Ltd.
publisher.none.fl_str_mv BioMed Central Ltd.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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