Molecular characterization of Brucella ovis in Argentina

Autores
Alvarez, Lucía Paula; Ruiz-Villalobos, Nazaret; Suárez-Esquivel, Marcela; Thomson, Nicholas R.; Marcellino, Romanela; Víquez-Ruiz, Eunice; Robles, Carlos Alejandro; Guzmán-Verri, Caterina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now.
Estación Experimental Agropecuaria Bariloche
Fil: Alvarez, Lucía Paula. Universidad Nacional del Comahue. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Ruiz-Villalobos, Nazaret. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica
Fil: Suárez-Esquivel, Marcela. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica
Fil: Thomson, Nicholas R. Wellcome Trust Sanger Institute. Parasites and Microbes from Pathogen Genomics; Reino Unido
Fil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Sanidad Animal; Argentina
Fil: Víquez-Ruiz, Eunice. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area de Produccion Animal. Grupo de Sanidad Animal; Argentina
Fil: Guzmán-Verri, Caterina. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica
Fuente
Veterinary Microbiology 245 : Art. 108703 (June 2020)
Materia
Brucella Ovis
Brucella
Brucelosis
Brucella Melitensis
Genotipos
Enfermedades de los Animales
Brucellosis
Genotypes
Animal Diseases
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7928
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7928
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network_name_str INTA Digital (INTA)
spelling Molecular characterization of Brucella ovis in ArgentinaAlvarez, Lucía PaulaRuiz-Villalobos, NazaretSuárez-Esquivel, MarcelaThomson, Nicholas R.Marcellino, RomanelaVíquez-Ruiz, EuniceRobles, Carlos AlejandroGuzmán-Verri, CaterinaBrucella OvisBrucellaBrucelosisBrucella MelitensisGenotiposEnfermedades de los AnimalesBrucellosisGenotypesAnimal DiseasesArgentinaBrucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now.Estación Experimental Agropecuaria BarilocheFil: Alvarez, Lucía Paula. Universidad Nacional del Comahue. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Ruiz-Villalobos, Nazaret. Universidad Nacional. Escuela de Medicina Veterinaria; Costa RicaFil: Suárez-Esquivel, Marcela. Universidad Nacional. Escuela de Medicina Veterinaria; Costa RicaFil: Thomson, Nicholas R. Wellcome Trust Sanger Institute. Parasites and Microbes from Pathogen Genomics; Reino UnidoFil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Sanidad Animal; ArgentinaFil: Víquez-Ruiz, Eunice. Universidad Nacional. Escuela de Medicina Veterinaria; Costa RicaFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area de Produccion Animal. Grupo de Sanidad Animal; ArgentinaFil: Guzmán-Verri, Caterina. Universidad Nacional. Escuela de Medicina Veterinaria; Costa RicaElsevier2020-09-21T12:17:40Z2020-09-21T12:17:40Z2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7928https://www.sciencedirect.com/science/article/pii/S037811351931452X0378-1135https://doi.org/10.1016/j.vetmic.2020.108703Veterinary Microbiology 245 : Art. 108703 (June 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:53Zoai:localhost:20.500.12123/7928instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:54.065INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization of Brucella ovis in Argentina
title Molecular characterization of Brucella ovis in Argentina
spellingShingle Molecular characterization of Brucella ovis in Argentina
Alvarez, Lucía Paula
Brucella Ovis
Brucella
Brucelosis
Brucella Melitensis
Genotipos
Enfermedades de los Animales
Brucellosis
Genotypes
Animal Diseases
Argentina
title_short Molecular characterization of Brucella ovis in Argentina
title_full Molecular characterization of Brucella ovis in Argentina
title_fullStr Molecular characterization of Brucella ovis in Argentina
title_full_unstemmed Molecular characterization of Brucella ovis in Argentina
title_sort Molecular characterization of Brucella ovis in Argentina
dc.creator.none.fl_str_mv Alvarez, Lucía Paula
Ruiz-Villalobos, Nazaret
Suárez-Esquivel, Marcela
Thomson, Nicholas R.
Marcellino, Romanela
Víquez-Ruiz, Eunice
Robles, Carlos Alejandro
Guzmán-Verri, Caterina
author Alvarez, Lucía Paula
author_facet Alvarez, Lucía Paula
Ruiz-Villalobos, Nazaret
Suárez-Esquivel, Marcela
Thomson, Nicholas R.
Marcellino, Romanela
Víquez-Ruiz, Eunice
Robles, Carlos Alejandro
Guzmán-Verri, Caterina
author_role author
author2 Ruiz-Villalobos, Nazaret
Suárez-Esquivel, Marcela
Thomson, Nicholas R.
Marcellino, Romanela
Víquez-Ruiz, Eunice
Robles, Carlos Alejandro
Guzmán-Verri, Caterina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Brucella Ovis
Brucella
Brucelosis
Brucella Melitensis
Genotipos
Enfermedades de los Animales
Brucellosis
Genotypes
Animal Diseases
Argentina
topic Brucella Ovis
Brucella
Brucelosis
Brucella Melitensis
Genotipos
Enfermedades de los Animales
Brucellosis
Genotypes
Animal Diseases
Argentina
dc.description.none.fl_txt_mv Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now.
Estación Experimental Agropecuaria Bariloche
Fil: Alvarez, Lucía Paula. Universidad Nacional del Comahue. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Ruiz-Villalobos, Nazaret. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica
Fil: Suárez-Esquivel, Marcela. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica
Fil: Thomson, Nicholas R. Wellcome Trust Sanger Institute. Parasites and Microbes from Pathogen Genomics; Reino Unido
Fil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Sanidad Animal; Argentina
Fil: Víquez-Ruiz, Eunice. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area de Produccion Animal. Grupo de Sanidad Animal; Argentina
Fil: Guzmán-Verri, Caterina. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica
description Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-21T12:17:40Z
2020-09-21T12:17:40Z
2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7928
https://www.sciencedirect.com/science/article/pii/S037811351931452X
0378-1135
https://doi.org/10.1016/j.vetmic.2020.108703
url http://hdl.handle.net/20.500.12123/7928
https://www.sciencedirect.com/science/article/pii/S037811351931452X
https://doi.org/10.1016/j.vetmic.2020.108703
identifier_str_mv 0378-1135
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Microbiology 245 : Art. 108703 (June 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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