Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
- Autores
- Beznec, Ailin; Faccio, Paula Daniela; Miralles, Daniel J.; Abeledo, Leonor Gabriela; Decima Oneto, Cecilia Andrea; Garibotto, María De Belén; Gonzalez, Germán Andrés; Moreyra, Federico; Elizondo, Matias; Ruiz, Monica Beatriz; Lewi, Dalia Marcela; Blumwald, Eduardo; Llorente, Berta; Diaz Paleo, Antonio Horacio; Bossio, Adrian Ezequiel
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.
EEA Pergamino
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina
Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina
Fil: Abeledo, Leonor G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina
Fil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Agrobiotecnología; Argentina
Fil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garibotto, María De Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Garibotto, María De Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Germán Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina
Fil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina
Fil: Elizondo, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Elizondo, Matias. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina
Fil: Ruiz, Mónica Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Ruiz, Mónica Beatriz. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos
Fil: Llorente, Berta. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; Argentina
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina - Fuente
- Journal of Genetic Engineering and Biotechnology 19 : 1-17. (May 2021)
- Materia
-
Trigo
Genética
Citoquininas
Estrés de Sequia
Wheat
Genetics
Cytokinins
Drought Stress - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/9683
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Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under droughtBeznec, AilinFaccio, Paula DanielaMiralles, Daniel J.Abeledo, Leonor GabrielaDecima Oneto, Cecilia AndreaGaribotto, María De BelénGonzalez, Germán AndrésMoreyra, FedericoElizondo, MatiasRuiz, Monica BeatrizLewi, Dalia MarcelaBlumwald, EduardoLlorente, BertaDiaz Paleo, Antonio HoracioBossio, Adrian EzequielTrigoGenéticaCitoquininasEstrés de SequiaWheatGeneticsCytokininsDrought StressBackground: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.EEA PergaminoFil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; ArgentinaFil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); ArgentinaFil: Abeledo, Leonor G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; ArgentinaFil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Agrobiotecnología; ArgentinaFil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garibotto, María De Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Garibotto, María De Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Germán Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; ArgentinaFil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; ArgentinaFil: Elizondo, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; ArgentinaFil: Elizondo, Matias. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; ArgentinaFil: Ruiz, Mónica Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; ArgentinaFil: Ruiz, Mónica Beatriz. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; ArgentinaFil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados UnidosFil: Llorente, Berta. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; ArgentinaFil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaSpringer2021-06-29T12:14:44Z2021-06-29T12:14:44Z2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9683https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-whttps://doi.org/10.1186/s43141-021-00171-wJournal of Genetic Engineering and Biotechnology 19 : 1-17. (May 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:15Zoai:localhost:20.500.12123/9683instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:16.009INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
title |
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
spellingShingle |
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought Beznec, Ailin Trigo Genética Citoquininas Estrés de Sequia Wheat Genetics Cytokinins Drought Stress |
title_short |
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
title_full |
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
title_fullStr |
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
title_full_unstemmed |
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
title_sort |
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
dc.creator.none.fl_str_mv |
Beznec, Ailin Faccio, Paula Daniela Miralles, Daniel J. Abeledo, Leonor Gabriela Decima Oneto, Cecilia Andrea Garibotto, María De Belén Gonzalez, Germán Andrés Moreyra, Federico Elizondo, Matias Ruiz, Monica Beatriz Lewi, Dalia Marcela Blumwald, Eduardo Llorente, Berta Diaz Paleo, Antonio Horacio Bossio, Adrian Ezequiel |
author |
Beznec, Ailin |
author_facet |
Beznec, Ailin Faccio, Paula Daniela Miralles, Daniel J. Abeledo, Leonor Gabriela Decima Oneto, Cecilia Andrea Garibotto, María De Belén Gonzalez, Germán Andrés Moreyra, Federico Elizondo, Matias Ruiz, Monica Beatriz Lewi, Dalia Marcela Blumwald, Eduardo Llorente, Berta Diaz Paleo, Antonio Horacio Bossio, Adrian Ezequiel |
author_role |
author |
author2 |
Faccio, Paula Daniela Miralles, Daniel J. Abeledo, Leonor Gabriela Decima Oneto, Cecilia Andrea Garibotto, María De Belén Gonzalez, Germán Andrés Moreyra, Federico Elizondo, Matias Ruiz, Monica Beatriz Lewi, Dalia Marcela Blumwald, Eduardo Llorente, Berta Diaz Paleo, Antonio Horacio Bossio, Adrian Ezequiel |
author2_role |
author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Trigo Genética Citoquininas Estrés de Sequia Wheat Genetics Cytokinins Drought Stress |
topic |
Trigo Genética Citoquininas Estrés de Sequia Wheat Genetics Cytokinins Drought Stress |
dc.description.none.fl_txt_mv |
Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions. EEA Pergamino Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina Fil: Abeledo, Leonor G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina Fil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Agrobiotecnología; Argentina Fil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garibotto, María De Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Garibotto, María De Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Germán Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina Fil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina Fil: Elizondo, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina Fil: Elizondo, Matias. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina Fil: Ruiz, Mónica Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina Fil: Ruiz, Mónica Beatriz. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos Fil: Llorente, Berta. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; Argentina Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina |
description |
Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-29T12:14:44Z 2021-06-29T12:14:44Z 2021-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/9683 https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-w https://doi.org/10.1186/s43141-021-00171-w |
url |
http://hdl.handle.net/20.500.12123/9683 https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-w https://doi.org/10.1186/s43141-021-00171-w |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos. |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Journal of Genetic Engineering and Biotechnology 19 : 1-17. (May 2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619155017302016 |
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12.559606 |