Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought

Autores
Beznec, Ailin; Faccio, Paula Daniela; Miralles, Daniel J.; Abeledo, Leonor Gabriela; Decima Oneto, Cecilia Andrea; Garibotto, María De Belén; Gonzalez, Germán Andrés; Moreyra, Federico; Elizondo, Matias; Ruiz, Monica Beatriz; Lewi, Dalia Marcela; Blumwald, Eduardo; Llorente, Berta; Diaz Paleo, Antonio Horacio; Bossio, Adrian Ezequiel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.
EEA Pergamino
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina
Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina
Fil: Abeledo, Leonor G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina
Fil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Agrobiotecnología; Argentina
Fil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garibotto, María De Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Garibotto, María De Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Germán Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina
Fil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina
Fil: Elizondo, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Elizondo, Matias. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina
Fil: Ruiz, Mónica Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Ruiz, Mónica Beatriz. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos
Fil: Llorente, Berta. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; Argentina
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
Journal of Genetic Engineering and Biotechnology 19 : 1-17. (May 2021)
Materia
Trigo
Genética
Citoquininas
Estrés de Sequia
Wheat
Genetics
Cytokinins
Drought Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9683

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network_name_str INTA Digital (INTA)
spelling Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under droughtBeznec, AilinFaccio, Paula DanielaMiralles, Daniel J.Abeledo, Leonor GabrielaDecima Oneto, Cecilia AndreaGaribotto, María De BelénGonzalez, Germán AndrésMoreyra, FedericoElizondo, MatiasRuiz, Monica BeatrizLewi, Dalia MarcelaBlumwald, EduardoLlorente, BertaDiaz Paleo, Antonio HoracioBossio, Adrian EzequielTrigoGenéticaCitoquininasEstrés de SequiaWheatGeneticsCytokininsDrought StressBackground: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.EEA PergaminoFil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; ArgentinaFil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); ArgentinaFil: Abeledo, Leonor G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; ArgentinaFil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Agrobiotecnología; ArgentinaFil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garibotto, María De Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Garibotto, María De Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Germán Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; ArgentinaFil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; ArgentinaFil: Elizondo, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; ArgentinaFil: Elizondo, Matias. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; ArgentinaFil: Ruiz, Mónica Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; ArgentinaFil: Ruiz, Mónica Beatriz. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; ArgentinaFil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados UnidosFil: Llorente, Berta. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; ArgentinaFil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaSpringer2021-06-29T12:14:44Z2021-06-29T12:14:44Z2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9683https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-whttps://doi.org/10.1186/s43141-021-00171-wJournal of Genetic Engineering and Biotechnology 19 : 1-17. (May 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:15Zoai:localhost:20.500.12123/9683instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:16.009INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
spellingShingle Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
Beznec, Ailin
Trigo
Genética
Citoquininas
Estrés de Sequia
Wheat
Genetics
Cytokinins
Drought Stress
title_short Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title_full Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title_fullStr Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title_full_unstemmed Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title_sort Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
dc.creator.none.fl_str_mv Beznec, Ailin
Faccio, Paula Daniela
Miralles, Daniel J.
Abeledo, Leonor Gabriela
Decima Oneto, Cecilia Andrea
Garibotto, María De Belén
Gonzalez, Germán Andrés
Moreyra, Federico
Elizondo, Matias
Ruiz, Monica Beatriz
Lewi, Dalia Marcela
Blumwald, Eduardo
Llorente, Berta
Diaz Paleo, Antonio Horacio
Bossio, Adrian Ezequiel
author Beznec, Ailin
author_facet Beznec, Ailin
Faccio, Paula Daniela
Miralles, Daniel J.
Abeledo, Leonor Gabriela
Decima Oneto, Cecilia Andrea
Garibotto, María De Belén
Gonzalez, Germán Andrés
Moreyra, Federico
Elizondo, Matias
Ruiz, Monica Beatriz
Lewi, Dalia Marcela
Blumwald, Eduardo
Llorente, Berta
Diaz Paleo, Antonio Horacio
Bossio, Adrian Ezequiel
author_role author
author2 Faccio, Paula Daniela
Miralles, Daniel J.
Abeledo, Leonor Gabriela
Decima Oneto, Cecilia Andrea
Garibotto, María De Belén
Gonzalez, Germán Andrés
Moreyra, Federico
Elizondo, Matias
Ruiz, Monica Beatriz
Lewi, Dalia Marcela
Blumwald, Eduardo
Llorente, Berta
Diaz Paleo, Antonio Horacio
Bossio, Adrian Ezequiel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trigo
Genética
Citoquininas
Estrés de Sequia
Wheat
Genetics
Cytokinins
Drought Stress
topic Trigo
Genética
Citoquininas
Estrés de Sequia
Wheat
Genetics
Cytokinins
Drought Stress
dc.description.none.fl_txt_mv Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.
EEA Pergamino
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina
Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina
Fil: Abeledo, Leonor G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina
Fil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Agrobiotecnología; Argentina
Fil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garibotto, María De Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Garibotto, María De Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Germán Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina
Fil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina
Fil: Elizondo, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Elizondo, Matias. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina
Fil: Ruiz, Mónica Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Ruiz, Mónica Beatriz. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos
Fil: Llorente, Berta. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; Argentina
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-29T12:14:44Z
2021-06-29T12:14:44Z
2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9683
https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-w
https://doi.org/10.1186/s43141-021-00171-w
url http://hdl.handle.net/20.500.12123/9683
https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-w
https://doi.org/10.1186/s43141-021-00171-w
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Genetic Engineering and Biotechnology 19 : 1-17. (May 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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