Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter

Autores
Decima Oneto, Cecilia Andrea; Otegui, Marí­a Elena; Baroli, Irene; Beznec, Ailin; Faccio, Paula Daniela; Bossio, Adrian Ezequiel; Blumwald, Eduardo; Lewi, Dalia Marcela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize.
Inst. de Genética "Ewald A. Favret"- IGEAF
Fil: Decima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Otegui, Marí­a Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Bossio, Adrian EzequielInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Blumwald, Eduardo. University of California. Department Plant Sciences; Estados Unidos
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fuente
Journal of biotechnology 220 : 66-77. (February 2016)
Materia
Genética
Agua
Estrés de Sequia
Maíz
Resistencia a la Sequía
Genetics
Water
Drought Stress
Maize
Drought Resistance
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1124
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1124
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoterDecima Oneto, Cecilia AndreaOtegui, Marí­a ElenaBaroli, IreneBeznec, AilinFaccio, Paula DanielaBossio, Adrian EzequielBlumwald, EduardoLewi, Dalia MarcelaGenéticaAguaEstrés de SequiaMaízResistencia a la SequíaGeneticsWaterDrought StressMaizeDrought ResistanceSenescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize.Inst. de Genética "Ewald A. Favret"- IGEAFFil: Decima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Otegui, Marí­a Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Bossio, Adrian EzequielInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Blumwald, Eduardo. University of California. Department Plant Sciences; Estados UnidosFil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina2017-09-05T12:41:08Z2017-09-05T12:41:08Z2016info:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1124http://www.sciencedirect.com/science/article/pii/S0168165616300165?via%3Dihub0168-1656 (Print)1873-4863 (Online)https://doi.org/10.1016/j.jbiotec.2016.01.014Journal of biotechnology 220 : 66-77. (February 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:09Zoai:localhost:20.500.12123/1124instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:10.195INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
title Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
spellingShingle Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
Decima Oneto, Cecilia Andrea
Genética
Agua
Estrés de Sequia
Maíz
Resistencia a la Sequía
Genetics
Water
Drought Stress
Maize
Drought Resistance
title_short Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
title_full Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
title_fullStr Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
title_full_unstemmed Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
title_sort Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
dc.creator.none.fl_str_mv Decima Oneto, Cecilia Andrea
Otegui, Marí­a Elena
Baroli, Irene
Beznec, Ailin
Faccio, Paula Daniela
Bossio, Adrian Ezequiel
Blumwald, Eduardo
Lewi, Dalia Marcela
author Decima Oneto, Cecilia Andrea
author_facet Decima Oneto, Cecilia Andrea
Otegui, Marí­a Elena
Baroli, Irene
Beznec, Ailin
Faccio, Paula Daniela
Bossio, Adrian Ezequiel
Blumwald, Eduardo
Lewi, Dalia Marcela
author_role author
author2 Otegui, Marí­a Elena
Baroli, Irene
Beznec, Ailin
Faccio, Paula Daniela
Bossio, Adrian Ezequiel
Blumwald, Eduardo
Lewi, Dalia Marcela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genética
Agua
Estrés de Sequia
Maíz
Resistencia a la Sequía
Genetics
Water
Drought Stress
Maize
Drought Resistance
topic Genética
Agua
Estrés de Sequia
Maíz
Resistencia a la Sequía
Genetics
Water
Drought Stress
Maize
Drought Resistance
dc.description.none.fl_txt_mv Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize.
Inst. de Genética "Ewald A. Favret"- IGEAF
Fil: Decima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Otegui, Marí­a Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Bossio, Adrian EzequielInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Blumwald, Eduardo. University of California. Department Plant Sciences; Estados Unidos
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
description Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-09-05T12:41:08Z
2017-09-05T12:41:08Z
dc.type.none.fl_str_mv info:eu-repo/semantics/acceptedVersion
info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
status_str acceptedVersion
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1124
http://www.sciencedirect.com/science/article/pii/S0168165616300165?via%3Dihub
0168-1656 (Print)
1873-4863 (Online)
https://doi.org/10.1016/j.jbiotec.2016.01.014
url http://hdl.handle.net/20.500.12123/1124
http://www.sciencedirect.com/science/article/pii/S0168165616300165?via%3Dihub
https://doi.org/10.1016/j.jbiotec.2016.01.014
identifier_str_mv 0168-1656 (Print)
1873-4863 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of biotechnology 220 : 66-77. (February 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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