Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought

Autores
Beznec, Ailin Yanina; Faccio, Paula Daniela; Miralles, Daniel Julio; Abeledo, Leonor Gabriela; Décima Oneto, Cecilia Andrea; Garibotto, Maria de Belen; Gonzalez, Germán; Moreyra, Federico; Elizondo, Matías; Ruíz, Mónica; Lewi, Dalia Marcela; Blumwald, Eduardo; Llorente, Berta Elizabet; Paleo, Antonio Díaz; Bossio, Ezequiel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.
Fil: Beznec, Ailin Yanina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo | Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Abeledo, Leonor Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina
Fil: Garibotto, Maria de Belen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo | Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo; Argentina
Fil: Gonzalez, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Elizondo, Matías. Universidad Nacional de San Juan; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Ruíz, Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Universidad Nacional de San Juan; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Blumwald, Eduardo. University of California at Davis; Estados Unidos
Fil: Llorente, Berta Elizabet. Universidad Nacional de Luján; Argentina
Fil: Paleo, Antonio Díaz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Bossio, Ezequiel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Materia
AGRONOMIC TRAITS
CYTOKININ
GENETIC TRANSFORMATION
ISOPENTENYL TRANSFERASE GENE
TRITICUM AESTIVUM L
WATER-DEFICIT TOLERANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168114
Acceder
id CONICETDig_809782ee909d5d5403a62d86ada5a225
oai_identifier_str oai:ri.conicet.gov.ar:11336/168114
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under droughtBeznec, Ailin YaninaFaccio, Paula DanielaMiralles, Daniel JulioAbeledo, Leonor GabrielaDécima Oneto, Cecilia AndreaGaribotto, Maria de BelenGonzalez, GermánMoreyra, FedericoElizondo, MatíasRuíz, MónicaLewi, Dalia MarcelaBlumwald, EduardoLlorente, Berta ElizabetPaleo, Antonio DíazBossio, EzequielAGRONOMIC TRAITSCYTOKININGENETIC TRANSFORMATIONISOPENTENYL TRANSFERASE GENETRITICUM AESTIVUM LWATER-DEFICIT TOLERANCEhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.Fil: Beznec, Ailin Yanina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo | Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo; ArgentinaFil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Abeledo, Leonor Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; ArgentinaFil: Garibotto, Maria de Belen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo | Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo; ArgentinaFil: Gonzalez, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Elizondo, Matías. Universidad Nacional de San Juan; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Ruíz, Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Universidad Nacional de San Juan; ArgentinaFil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Blumwald, Eduardo. University of California at Davis; Estados UnidosFil: Llorente, Berta Elizabet. Universidad Nacional de Luján; ArgentinaFil: Paleo, Antonio Díaz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Bossio, Ezequiel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaSpringer2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168114Beznec, Ailin Yanina; Faccio, Paula Daniela; Miralles, Daniel Julio; Abeledo, Leonor Gabriela; Décima Oneto, Cecilia Andrea; et al.; Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought; Springer; Journal of Genetic Engineering and Biotechnology; 19; 67; 12-2021; 1-171687-157X2090-5920CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s43141-021-00171-winfo:eu-repo/semantics/altIdentifier/url/https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:42:00Zoai:ri.conicet.gov.ar:11336/168114instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:42:00.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
spellingShingle Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
Beznec, Ailin Yanina
AGRONOMIC TRAITS
CYTOKININ
GENETIC TRANSFORMATION
ISOPENTENYL TRANSFERASE GENE
TRITICUM AESTIVUM L
WATER-DEFICIT TOLERANCE
title_short Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title_full Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title_fullStr Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title_full_unstemmed Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
title_sort Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
dc.creator.none.fl_str_mv Beznec, Ailin Yanina
Faccio, Paula Daniela
Miralles, Daniel Julio
Abeledo, Leonor Gabriela
Décima Oneto, Cecilia Andrea
Garibotto, Maria de Belen
Gonzalez, Germán
Moreyra, Federico
Elizondo, Matías
Ruíz, Mónica
Lewi, Dalia Marcela
Blumwald, Eduardo
Llorente, Berta Elizabet
Paleo, Antonio Díaz
Bossio, Ezequiel
author Beznec, Ailin Yanina
author_facet Beznec, Ailin Yanina
Faccio, Paula Daniela
Miralles, Daniel Julio
Abeledo, Leonor Gabriela
Décima Oneto, Cecilia Andrea
Garibotto, Maria de Belen
Gonzalez, Germán
Moreyra, Federico
Elizondo, Matías
Ruíz, Mónica
Lewi, Dalia Marcela
Blumwald, Eduardo
Llorente, Berta Elizabet
Paleo, Antonio Díaz
Bossio, Ezequiel
author_role author
author2 Faccio, Paula Daniela
Miralles, Daniel Julio
Abeledo, Leonor Gabriela
Décima Oneto, Cecilia Andrea
Garibotto, Maria de Belen
Gonzalez, Germán
Moreyra, Federico
Elizondo, Matías
Ruíz, Mónica
Lewi, Dalia Marcela
Blumwald, Eduardo
Llorente, Berta Elizabet
Paleo, Antonio Díaz
Bossio, Ezequiel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AGRONOMIC TRAITS
CYTOKININ
GENETIC TRANSFORMATION
ISOPENTENYL TRANSFERASE GENE
TRITICUM AESTIVUM L
WATER-DEFICIT TOLERANCE
topic AGRONOMIC TRAITS
CYTOKININ
GENETIC TRANSFORMATION
ISOPENTENYL TRANSFERASE GENE
TRITICUM AESTIVUM L
WATER-DEFICIT TOLERANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.
Fil: Beznec, Ailin Yanina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo | Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Abeledo, Leonor Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina
Fil: Garibotto, Maria de Belen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo | Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular. Grupo Vinculado Instituto de Genética "Ewald A. Favret" al Iabimo; Argentina
Fil: Gonzalez, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Elizondo, Matías. Universidad Nacional de San Juan; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Ruíz, Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Universidad Nacional de San Juan; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Blumwald, Eduardo. University of California at Davis; Estados Unidos
Fil: Llorente, Berta Elizabet. Universidad Nacional de Luján; Argentina
Fil: Paleo, Antonio Díaz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Bossio, Ezequiel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
description Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168114
Beznec, Ailin Yanina; Faccio, Paula Daniela; Miralles, Daniel Julio; Abeledo, Leonor Gabriela; Décima Oneto, Cecilia Andrea; et al.; Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought; Springer; Journal of Genetic Engineering and Biotechnology; 19; 67; 12-2021; 1-17
1687-157X
2090-5920
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168114
identifier_str_mv Beznec, Ailin Yanina; Faccio, Paula Daniela; Miralles, Daniel Julio; Abeledo, Leonor Gabriela; Décima Oneto, Cecilia Andrea; et al.; Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought; Springer; Journal of Genetic Engineering and Biotechnology; 19; 67; 12-2021; 1-17
1687-157X
2090-5920
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s43141-021-00171-w
info:eu-repo/semantics/altIdentifier/url/https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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