Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics

Autores
Ferre, Luis Bernardo; Malik, Genoveva; Aller Atucha, Juan Florencio; Alberio, Ricardo; Fresno, Cristóbal; Kjelland, Michael
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable and repeatable semen collection technique for Llama, (b) validate semen evaluation tests for Llama, and (c) to determine the effect of collection frequency and season on Llama semen characteristics. First, the semen and sperm variables were recorded and validated through their own repeatability. Semen collection intervals tested were: 1X/week for three weeks and 3X/week for another three weeks, the second collection period occurring after two weeks of sexual rest. The collection frequency of 3X/week significantly reduced (P < 0.05) the sperm viability, motility, concentration, Total Sperm/Ejaculate, Total Motile Sperm/Ejaculate and Total Live Sperm/Ejaculate, but improved Total Sperm/Week and Total Live Sperm/Week. All recorded variables were significantly higher during the summer in comparison to the spring with the exception of morphology abnormalities, volume, and viability. Also, the 1X/week versus the 3X/week semen collection frequencies did not produce a significant difference in the percent of total motile sperm/week. Based on semen and sperm characteristics evaluated herein, Llama semen collected in the summer was better (P < 0.05), with regard to the majority of the variables analyzed, than semen collected in the spring.
EEA Balcarce
Fil: Ferré Luis Bernardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Malik, Genoveva. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Fresno, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba. Facultad de Ingeniería; Argentina.
Fil: Kjelland, Michael. Conservation, Genetics & Biotech; Estados Unidos.
Fuente
Small Ruminant Research 133 : 140-147 (december 2015)
Materia
Llama
Eyaculación
Recolección de Semen
Evaluación
Espermatozoo
Estacionalidad
Llamas
Ejaculation
Semen Collection
Evaluation
Spermatozoa
Seasonality
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10753
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10753
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristicsFerre, Luis BernardoMalik, GenovevaAller Atucha, Juan FlorencioAlberio, RicardoFresno, CristóbalKjelland, MichaelLlamaEyaculaciónRecolección de SemenEvaluaciónEspermatozooEstacionalidadLlamasEjaculationSemen CollectionEvaluationSpermatozoaSeasonalityAdult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable and repeatable semen collection technique for Llama, (b) validate semen evaluation tests for Llama, and (c) to determine the effect of collection frequency and season on Llama semen characteristics. First, the semen and sperm variables were recorded and validated through their own repeatability. Semen collection intervals tested were: 1X/week for three weeks and 3X/week for another three weeks, the second collection period occurring after two weeks of sexual rest. The collection frequency of 3X/week significantly reduced (P < 0.05) the sperm viability, motility, concentration, Total Sperm/Ejaculate, Total Motile Sperm/Ejaculate and Total Live Sperm/Ejaculate, but improved Total Sperm/Week and Total Live Sperm/Week. All recorded variables were significantly higher during the summer in comparison to the spring with the exception of morphology abnormalities, volume, and viability. Also, the 1X/week versus the 3X/week semen collection frequencies did not produce a significant difference in the percent of total motile sperm/week. Based on semen and sperm characteristics evaluated herein, Llama semen collected in the summer was better (P < 0.05), with regard to the majority of the variables analyzed, than semen collected in the spring.EEA BalcarceFil: Ferré Luis Bernardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Malik, Genoveva. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Fresno, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba. Facultad de Ingeniería; Argentina.Fil: Kjelland, Michael. Conservation, Genetics & Biotech; Estados Unidos.Elsevier2021-11-11T17:18:25Z2021-11-11T17:18:25Z2015-09-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10753https://www.sciencedirect.com/science/article/abs/pii/S09214488153005230921-4488https://doi.org/10.1016/j.smallrumres.2015.08.016Small Ruminant Research 133 : 140-147 (december 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:24Zoai:localhost:20.500.12123/10753instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:24.922INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
title Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
spellingShingle Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
Ferre, Luis Bernardo
Llama
Eyaculación
Recolección de Semen
Evaluación
Espermatozoo
Estacionalidad
Llamas
Ejaculation
Semen Collection
Evaluation
Spermatozoa
Seasonality
title_short Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
title_full Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
title_fullStr Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
title_full_unstemmed Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
title_sort Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
dc.creator.none.fl_str_mv Ferre, Luis Bernardo
Malik, Genoveva
Aller Atucha, Juan Florencio
Alberio, Ricardo
Fresno, Cristóbal
Kjelland, Michael
author Ferre, Luis Bernardo
author_facet Ferre, Luis Bernardo
Malik, Genoveva
Aller Atucha, Juan Florencio
Alberio, Ricardo
Fresno, Cristóbal
Kjelland, Michael
author_role author
author2 Malik, Genoveva
Aller Atucha, Juan Florencio
Alberio, Ricardo
Fresno, Cristóbal
Kjelland, Michael
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Llama
Eyaculación
Recolección de Semen
Evaluación
Espermatozoo
Estacionalidad
Llamas
Ejaculation
Semen Collection
Evaluation
Spermatozoa
Seasonality
topic Llama
Eyaculación
Recolección de Semen
Evaluación
Espermatozoo
Estacionalidad
Llamas
Ejaculation
Semen Collection
Evaluation
Spermatozoa
Seasonality
dc.description.none.fl_txt_mv Adult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable and repeatable semen collection technique for Llama, (b) validate semen evaluation tests for Llama, and (c) to determine the effect of collection frequency and season on Llama semen characteristics. First, the semen and sperm variables were recorded and validated through their own repeatability. Semen collection intervals tested were: 1X/week for three weeks and 3X/week for another three weeks, the second collection period occurring after two weeks of sexual rest. The collection frequency of 3X/week significantly reduced (P < 0.05) the sperm viability, motility, concentration, Total Sperm/Ejaculate, Total Motile Sperm/Ejaculate and Total Live Sperm/Ejaculate, but improved Total Sperm/Week and Total Live Sperm/Week. All recorded variables were significantly higher during the summer in comparison to the spring with the exception of morphology abnormalities, volume, and viability. Also, the 1X/week versus the 3X/week semen collection frequencies did not produce a significant difference in the percent of total motile sperm/week. Based on semen and sperm characteristics evaluated herein, Llama semen collected in the summer was better (P < 0.05), with regard to the majority of the variables analyzed, than semen collected in the spring.
EEA Balcarce
Fil: Ferré Luis Bernardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Malik, Genoveva. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Fresno, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba. Facultad de Ingeniería; Argentina.
Fil: Kjelland, Michael. Conservation, Genetics & Biotech; Estados Unidos.
description Adult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable and repeatable semen collection technique for Llama, (b) validate semen evaluation tests for Llama, and (c) to determine the effect of collection frequency and season on Llama semen characteristics. First, the semen and sperm variables were recorded and validated through their own repeatability. Semen collection intervals tested were: 1X/week for three weeks and 3X/week for another three weeks, the second collection period occurring after two weeks of sexual rest. The collection frequency of 3X/week significantly reduced (P < 0.05) the sperm viability, motility, concentration, Total Sperm/Ejaculate, Total Motile Sperm/Ejaculate and Total Live Sperm/Ejaculate, but improved Total Sperm/Week and Total Live Sperm/Week. All recorded variables were significantly higher during the summer in comparison to the spring with the exception of morphology abnormalities, volume, and viability. Also, the 1X/week versus the 3X/week semen collection frequencies did not produce a significant difference in the percent of total motile sperm/week. Based on semen and sperm characteristics evaluated herein, Llama semen collected in the summer was better (P < 0.05), with regard to the majority of the variables analyzed, than semen collected in the spring.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-09
2021-11-11T17:18:25Z
2021-11-11T17:18:25Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10753
https://www.sciencedirect.com/science/article/abs/pii/S0921448815300523
0921-4488
https://doi.org/10.1016/j.smallrumres.2015.08.016
url http://hdl.handle.net/20.500.12123/10753
https://www.sciencedirect.com/science/article/abs/pii/S0921448815300523
https://doi.org/10.1016/j.smallrumres.2015.08.016
identifier_str_mv 0921-4488
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Small Ruminant Research 133 : 140-147 (december 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.891075

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