Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa

Autores
Carretero, Maria Ignacia; Neild, Debora Margarita; Ferrante, Alejandro Antonio; Caldevilla, M.; Arraztoa, Claudia Cecilia; Fumuso, Fernanda Gabriela; Giuliano, Susana María
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to determine the effect of two equilibration tempera- tures (5 °C and room temperature) and two cryoprotectants (glycerol and dimethylformamide, both at 7%) on llama sperm cryopreservation. Llama ejac- ulates were divided into four aliquots. A lactose-EDTA-egg yolk (LEEY) exten- der with either 7% glycerol (LEEY-G) or 7% dimethylformamide (LEEY-DMF) was added to two of the aliquots, which were equilibrated for 20 min at room temperature and subsequently frozen. The other two aliquots were extended in LEEY, cooled to 5 °C, then LEEY-G or LEEY-DMF was added, equilibrated for 20 min at 5 °C and frozen. No significant differences (P > 0.05) were observed in membrane function and chromatin condensation between any of the freeze? thawing protocols. Post-thaw motility was greater (P < 0.05) in LEEY-DMF than LEEY-G. DNA fragmentation was not different between raw and frozen semen with LEEY-DMF but was high in all samples with glycerol. Our results indicate that 7% glycerol would be detrimental for llama spermatozoa, but fur- ther studies are needed to evaluate effectiveness if used at lower concentrations. Dimethylformamide preserved motility and DNA integrity of frozen?thawed llama spermatozoa and could be used to replace glycerol at the concentrations used in this study.
Fil: Carretero, Maria Ignacia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Neild, Debora Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Caldevilla, M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Arraztoa, Claudia Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Giuliano, Susana María. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Materia
CRYOPRESERVATION
DIMETHYFORMAMIDE
GLYCEROL
LAMA GLAMA (LLAMA)
SEMEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118302
Acceder
id CONICETDig_99ffdbfdf84bc00d8fe7582760380a3d
oai_identifier_str oai:ri.conicet.gov.ar:11336/118302
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoaCarretero, Maria IgnaciaNeild, Debora MargaritaFerrante, Alejandro AntonioCaldevilla, M.Arraztoa, Claudia CeciliaFumuso, Fernanda GabrielaGiuliano, Susana MaríaCRYOPRESERVATIONDIMETHYFORMAMIDEGLYCEROLLAMA GLAMA (LLAMA)SEMENhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to determine the effect of two equilibration tempera- tures (5 °C and room temperature) and two cryoprotectants (glycerol and dimethylformamide, both at 7%) on llama sperm cryopreservation. Llama ejac- ulates were divided into four aliquots. A lactose-EDTA-egg yolk (LEEY) exten- der with either 7% glycerol (LEEY-G) or 7% dimethylformamide (LEEY-DMF) was added to two of the aliquots, which were equilibrated for 20 min at room temperature and subsequently frozen. The other two aliquots were extended in LEEY, cooled to 5 °C, then LEEY-G or LEEY-DMF was added, equilibrated for 20 min at 5 °C and frozen. No significant differences (P > 0.05) were observed in membrane function and chromatin condensation between any of the freeze? thawing protocols. Post-thaw motility was greater (P < 0.05) in LEEY-DMF than LEEY-G. DNA fragmentation was not different between raw and frozen semen with LEEY-DMF but was high in all samples with glycerol. Our results indicate that 7% glycerol would be detrimental for llama spermatozoa, but fur- ther studies are needed to evaluate effectiveness if used at lower concentrations. Dimethylformamide preserved motility and DNA integrity of frozen?thawed llama spermatozoa and could be used to replace glycerol at the concentrations used in this study.Fil: Carretero, Maria Ignacia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Neild, Debora Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Caldevilla, M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Arraztoa, Claudia Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Giuliano, Susana María. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaWiley Blackwell Publishing, Inc2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118302Carretero, Maria Ignacia; Neild, Debora Margarita; Ferrante, Alejandro Antonio; Caldevilla, M.; Arraztoa, Claudia Cecilia; et al.; Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa; Wiley Blackwell Publishing, Inc; Andrologia; 47; 6; 8-2015; 685-6930303-4569CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12319info:eu-repo/semantics/altIdentifier/doi/10.1111/and.12319info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:08Zoai:ri.conicet.gov.ar:11336/118302instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:08.49CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
title Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
spellingShingle Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
Carretero, Maria Ignacia
CRYOPRESERVATION
DIMETHYFORMAMIDE
GLYCEROL
LAMA GLAMA (LLAMA)
SEMEN
title_short Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
title_full Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
title_fullStr Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
title_full_unstemmed Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
title_sort Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
dc.creator.none.fl_str_mv Carretero, Maria Ignacia
Neild, Debora Margarita
Ferrante, Alejandro Antonio
Caldevilla, M.
Arraztoa, Claudia Cecilia
Fumuso, Fernanda Gabriela
Giuliano, Susana María
author Carretero, Maria Ignacia
author_facet Carretero, Maria Ignacia
Neild, Debora Margarita
Ferrante, Alejandro Antonio
Caldevilla, M.
Arraztoa, Claudia Cecilia
Fumuso, Fernanda Gabriela
Giuliano, Susana María
author_role author
author2 Neild, Debora Margarita
Ferrante, Alejandro Antonio
Caldevilla, M.
Arraztoa, Claudia Cecilia
Fumuso, Fernanda Gabriela
Giuliano, Susana María
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CRYOPRESERVATION
DIMETHYFORMAMIDE
GLYCEROL
LAMA GLAMA (LLAMA)
SEMEN
topic CRYOPRESERVATION
DIMETHYFORMAMIDE
GLYCEROL
LAMA GLAMA (LLAMA)
SEMEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to determine the effect of two equilibration tempera- tures (5 °C and room temperature) and two cryoprotectants (glycerol and dimethylformamide, both at 7%) on llama sperm cryopreservation. Llama ejac- ulates were divided into four aliquots. A lactose-EDTA-egg yolk (LEEY) exten- der with either 7% glycerol (LEEY-G) or 7% dimethylformamide (LEEY-DMF) was added to two of the aliquots, which were equilibrated for 20 min at room temperature and subsequently frozen. The other two aliquots were extended in LEEY, cooled to 5 °C, then LEEY-G or LEEY-DMF was added, equilibrated for 20 min at 5 °C and frozen. No significant differences (P > 0.05) were observed in membrane function and chromatin condensation between any of the freeze? thawing protocols. Post-thaw motility was greater (P < 0.05) in LEEY-DMF than LEEY-G. DNA fragmentation was not different between raw and frozen semen with LEEY-DMF but was high in all samples with glycerol. Our results indicate that 7% glycerol would be detrimental for llama spermatozoa, but fur- ther studies are needed to evaluate effectiveness if used at lower concentrations. Dimethylformamide preserved motility and DNA integrity of frozen?thawed llama spermatozoa and could be used to replace glycerol at the concentrations used in this study.
Fil: Carretero, Maria Ignacia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Neild, Debora Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Caldevilla, M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Arraztoa, Claudia Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Giuliano, Susana María. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
description The aim of this study was to determine the effect of two equilibration tempera- tures (5 °C and room temperature) and two cryoprotectants (glycerol and dimethylformamide, both at 7%) on llama sperm cryopreservation. Llama ejac- ulates were divided into four aliquots. A lactose-EDTA-egg yolk (LEEY) exten- der with either 7% glycerol (LEEY-G) or 7% dimethylformamide (LEEY-DMF) was added to two of the aliquots, which were equilibrated for 20 min at room temperature and subsequently frozen. The other two aliquots were extended in LEEY, cooled to 5 °C, then LEEY-G or LEEY-DMF was added, equilibrated for 20 min at 5 °C and frozen. No significant differences (P > 0.05) were observed in membrane function and chromatin condensation between any of the freeze? thawing protocols. Post-thaw motility was greater (P < 0.05) in LEEY-DMF than LEEY-G. DNA fragmentation was not different between raw and frozen semen with LEEY-DMF but was high in all samples with glycerol. Our results indicate that 7% glycerol would be detrimental for llama spermatozoa, but fur- ther studies are needed to evaluate effectiveness if used at lower concentrations. Dimethylformamide preserved motility and DNA integrity of frozen?thawed llama spermatozoa and could be used to replace glycerol at the concentrations used in this study.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118302
Carretero, Maria Ignacia; Neild, Debora Margarita; Ferrante, Alejandro Antonio; Caldevilla, M.; Arraztoa, Claudia Cecilia; et al.; Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa; Wiley Blackwell Publishing, Inc; Andrologia; 47; 6; 8-2015; 685-693
0303-4569
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118302
identifier_str_mv Carretero, Maria Ignacia; Neild, Debora Margarita; Ferrante, Alejandro Antonio; Caldevilla, M.; Arraztoa, Claudia Cecilia; et al.; Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa; Wiley Blackwell Publishing, Inc; Andrologia; 47; 6; 8-2015; 685-693
0303-4569
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12319
info:eu-repo/semantics/altIdentifier/doi/10.1111/and.12319
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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