The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
- Autores
- Jorge, V.R.; Silva, M.R.; Guillin, Eduardo Alejandro; Freire, M.C.M.; Schuster, I.; Almeida, A.M.R.; Oliveira, Luiz O.
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations.
Instituto de Genética
Fil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; Brasil
Fil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; Brasil
Fil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil - Fuente
- Plant Pathology 64 (3) : 729-737 (June 2015)
- Materia
-
Soja
Enfermedades de las Plantas
Roya
Phakopsora pachyrhizi
Variación Genética
América del Sur
Soybeans
Plant Diseases
Rusts
Genetic Variation
South America
Roya de la Soja
Roya Asiática
Diversidad Genética - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/5140
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The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South AmericaJorge, V.R.Silva, M.R.Guillin, Eduardo AlejandroFreire, M.C.M.Schuster, I.Almeida, A.M.R.Oliveira, Luiz O.SojaEnfermedades de las PlantasRoyaPhakopsora pachyrhiziVariación GenéticaAmérica del SurSoybeansPlant DiseasesRustsGenetic VariationSouth AmericaRoya de la SojaRoya AsiáticaDiversidad GenéticaA sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations.Instituto de GenéticaFil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; BrasilFil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; BrasilFil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; BrasilFil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; BrasilFil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; BrasilFil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; BrasilWiley2019-05-17T13:03:49Z2019-05-17T13:03:49Z2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300http://hdl.handle.net/20.500.12123/51400032-08621365-3059https://doi.org/10.1111/ppa.12300Plant Pathology 64 (3) : 729-737 (June 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:40Zoai:localhost:20.500.12123/5140instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:40.332INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
title |
The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
spellingShingle |
The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America Jorge, V.R. Soja Enfermedades de las Plantas Roya Phakopsora pachyrhizi Variación Genética América del Sur Soybeans Plant Diseases Rusts Genetic Variation South America Roya de la Soja Roya Asiática Diversidad Genética |
title_short |
The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
title_full |
The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
title_fullStr |
The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
title_full_unstemmed |
The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
title_sort |
The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
dc.creator.none.fl_str_mv |
Jorge, V.R. Silva, M.R. Guillin, Eduardo Alejandro Freire, M.C.M. Schuster, I. Almeida, A.M.R. Oliveira, Luiz O. |
author |
Jorge, V.R. |
author_facet |
Jorge, V.R. Silva, M.R. Guillin, Eduardo Alejandro Freire, M.C.M. Schuster, I. Almeida, A.M.R. Oliveira, Luiz O. |
author_role |
author |
author2 |
Silva, M.R. Guillin, Eduardo Alejandro Freire, M.C.M. Schuster, I. Almeida, A.M.R. Oliveira, Luiz O. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Soja Enfermedades de las Plantas Roya Phakopsora pachyrhizi Variación Genética América del Sur Soybeans Plant Diseases Rusts Genetic Variation South America Roya de la Soja Roya Asiática Diversidad Genética |
topic |
Soja Enfermedades de las Plantas Roya Phakopsora pachyrhizi Variación Genética América del Sur Soybeans Plant Diseases Rusts Genetic Variation South America Roya de la Soja Roya Asiática Diversidad Genética |
dc.description.none.fl_txt_mv |
A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations. Instituto de Genética Fil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; Brasil Fil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; Brasil Fil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil |
description |
A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06 2019-05-17T13:03:49Z 2019-05-17T13:03:49Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300 http://hdl.handle.net/20.500.12123/5140 0032-0862 1365-3059 https://doi.org/10.1111/ppa.12300 |
url |
https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300 http://hdl.handle.net/20.500.12123/5140 https://doi.org/10.1111/ppa.12300 |
identifier_str_mv |
0032-0862 1365-3059 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
Plant Pathology 64 (3) : 729-737 (June 2015) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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