The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America

Autores
Jorge, V.R.; Silva, M.R.; Guillin, Eduardo Alejandro; Freire, M.C.M.; Schuster, I.; Almeida, A.M.R.; Oliveira, Luiz O.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations.
Instituto de Genética
Fil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; Brasil
Fil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; Brasil
Fil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fuente
Plant Pathology 64 (3) : 729-737 (June 2015)
Materia
Soja
Enfermedades de las Plantas
Roya
Phakopsora pachyrhizi
Variación Genética
América del Sur
Soybeans
Plant Diseases
Rusts
Genetic Variation
South America
Roya de la Soja
Roya Asiática
Diversidad Genética
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5140
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5140
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network_name_str INTA Digital (INTA)
spelling The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South AmericaJorge, V.R.Silva, M.R.Guillin, Eduardo AlejandroFreire, M.C.M.Schuster, I.Almeida, A.M.R.Oliveira, Luiz O.SojaEnfermedades de las PlantasRoyaPhakopsora pachyrhiziVariación GenéticaAmérica del SurSoybeansPlant DiseasesRustsGenetic VariationSouth AmericaRoya de la SojaRoya AsiáticaDiversidad GenéticaA sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations.Instituto de GenéticaFil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; BrasilFil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; BrasilFil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; BrasilFil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; BrasilFil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; BrasilFil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; BrasilWiley2019-05-17T13:03:49Z2019-05-17T13:03:49Z2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300http://hdl.handle.net/20.500.12123/51400032-08621365-3059https://doi.org/10.1111/ppa.12300Plant Pathology 64 (3) : 729-737 (June 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:57Zoai:localhost:20.500.12123/5140instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:57.371INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
title The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
spellingShingle The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
Jorge, V.R.
Soja
Enfermedades de las Plantas
Roya
Phakopsora pachyrhizi
Variación Genética
América del Sur
Soybeans
Plant Diseases
Rusts
Genetic Variation
South America
Roya de la Soja
Roya Asiática
Diversidad Genética
title_short The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
title_full The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
title_fullStr The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
title_full_unstemmed The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
title_sort The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
dc.creator.none.fl_str_mv Jorge, V.R.
Silva, M.R.
Guillin, Eduardo Alejandro
Freire, M.C.M.
Schuster, I.
Almeida, A.M.R.
Oliveira, Luiz O.
author Jorge, V.R.
author_facet Jorge, V.R.
Silva, M.R.
Guillin, Eduardo Alejandro
Freire, M.C.M.
Schuster, I.
Almeida, A.M.R.
Oliveira, Luiz O.
author_role author
author2 Silva, M.R.
Guillin, Eduardo Alejandro
Freire, M.C.M.
Schuster, I.
Almeida, A.M.R.
Oliveira, Luiz O.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Soja
Enfermedades de las Plantas
Roya
Phakopsora pachyrhizi
Variación Genética
América del Sur
Soybeans
Plant Diseases
Rusts
Genetic Variation
South America
Roya de la Soja
Roya Asiática
Diversidad Genética
topic Soja
Enfermedades de las Plantas
Roya
Phakopsora pachyrhizi
Variación Genética
América del Sur
Soybeans
Plant Diseases
Rusts
Genetic Variation
South America
Roya de la Soja
Roya Asiática
Diversidad Genética
dc.description.none.fl_txt_mv A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations.
Instituto de Genética
Fil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
Fil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; Brasil
Fil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; Brasil
Fil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil
description A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
2019-05-17T13:03:49Z
2019-05-17T13:03:49Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300
http://hdl.handle.net/20.500.12123/5140
0032-0862
1365-3059
https://doi.org/10.1111/ppa.12300
url https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300
http://hdl.handle.net/20.500.12123/5140
https://doi.org/10.1111/ppa.12300
identifier_str_mv 0032-0862
1365-3059
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant Pathology 64 (3) : 729-737 (June 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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