Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter

Autores
Poulsen Hornum, Anabela; Camadro, Elsa Lucila
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Crop wild relatives are conserved in germplasm banks as original accessions or their ex situ regenerated progenies. The commonly used sexual regeneration method in wild potatoes (FAO 2013)—cultivation of 20–25 plants/accession (N), hand-pollination with pollen mixtures when a percentage of the plants are in bloom, and composition of the regenerated accession without controlling the number of seeds provided by each female progenitor—does not consider the action of internal reproductive barriers. However, wild potatoes—mostly diploid and obligate out-crossers—may present internal pre-zygotic, post-zygotic, or both types of complete or incomplete reproductive barriers, which can reduce the effective number of progenitors (Ne) in relation to N. To evaluate possible phenotypic and genetic changes during a sexual reproduction cycle, two regeneration protocols were followed with one accession of Solanum chacoense Bitter: the current one and the proposed by Camadro (2012). The original accession and the two regenerated populations were morphologically and molecularly characterized. Twenty-one morphological characters (quantitative and qualitative) were considered, and the data were subjected to univariate and multivariate analyses. Allele frequencies were calculated for six microsatellite markers (SSR) and compared among populations with a chi-square test (α = 0.05). Great morphological variability for morphological phenotypes and four of the six analyzed SSR, as well as changes in allele frequencies (including allele loss) were revealed within and among the three populations. Therefore, it is advisable to consider aspects of reproductive and population genetics in developing regeneration protocols to prevent or reduce the risks of genetic erosion in sexual regeneration cycles.
EEA Balcarce
Fil: Poulsen Hornum, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada; Argentina
Fil: Poulsen Hornum, Anabela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Unidad Integrada; Argentina
Fil: Poulsen Hornum, Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada; Argentina
Fil: Camadro, Elsa Lucila. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Unidad Integrada; Argentina
Fil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Genetic Resources and Crop Evolution 71 : 2467-2484 (2024)
Materia
Solanum chacoense
Germplasm
Germplasm Banks
Germplasm Conservation
Potatoes
Germoplasma
Banco de Germoplasma
Conservación del Germoplasma
Papa
Papa Silvestre
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25407
Acceder
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oai_identifier_str oai:localhost:20.500.12123/25407
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense BitterPoulsen Hornum, AnabelaCamadro, Elsa LucilaSolanum chacoenseGermplasmGermplasm BanksGermplasm ConservationPotatoesGermoplasmaBanco de GermoplasmaConservación del GermoplasmaPapaPapa SilvestreCrop wild relatives are conserved in germplasm banks as original accessions or their ex situ regenerated progenies. The commonly used sexual regeneration method in wild potatoes (FAO 2013)—cultivation of 20–25 plants/accession (N), hand-pollination with pollen mixtures when a percentage of the plants are in bloom, and composition of the regenerated accession without controlling the number of seeds provided by each female progenitor—does not consider the action of internal reproductive barriers. However, wild potatoes—mostly diploid and obligate out-crossers—may present internal pre-zygotic, post-zygotic, or both types of complete or incomplete reproductive barriers, which can reduce the effective number of progenitors (Ne) in relation to N. To evaluate possible phenotypic and genetic changes during a sexual reproduction cycle, two regeneration protocols were followed with one accession of Solanum chacoense Bitter: the current one and the proposed by Camadro (2012). The original accession and the two regenerated populations were morphologically and molecularly characterized. Twenty-one morphological characters (quantitative and qualitative) were considered, and the data were subjected to univariate and multivariate analyses. Allele frequencies were calculated for six microsatellite markers (SSR) and compared among populations with a chi-square test (α = 0.05). Great morphological variability for morphological phenotypes and four of the six analyzed SSR, as well as changes in allele frequencies (including allele loss) were revealed within and among the three populations. Therefore, it is advisable to consider aspects of reproductive and population genetics in developing regeneration protocols to prevent or reduce the risks of genetic erosion in sexual regeneration cycles.EEA BalcarceFil: Poulsen Hornum, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada; ArgentinaFil: Poulsen Hornum, Anabela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Unidad Integrada; ArgentinaFil: Poulsen Hornum, Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada; ArgentinaFil: Camadro, Elsa Lucila. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Unidad Integrada; ArgentinaFil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2026-03-10T11:13:03Z2026-03-10T11:13:03Z2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25407https://link.springer.com/article/10.1007/s10722-023-01742-10925-9864 (print)1573-5109 (online)https://doi.org/10.1007/s10722-023-01742-1Genetic Resources and Crop Evolution 71 : 2467-2484 (2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNHFA-1106074/AR./Bases para la sostenibilidad de las cadenas de la papa y la batata.info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-03-26T11:25:30Zoai:localhost:20.500.12123/25407instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-03-26 11:25:30.375INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter
title Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter
spellingShingle Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter
Poulsen Hornum, Anabela
Solanum chacoense
Germplasm
Germplasm Banks
Germplasm Conservation
Potatoes
Germoplasma
Banco de Germoplasma
Conservación del Germoplasma
Papa
Papa Silvestre
title_short Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter
title_full Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter
title_fullStr Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter
title_full_unstemmed Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter
title_sort Morphological and molecular characterization of two populations ex situ regenerated from a germplasm bank accession of the wild potato Solanum chacoense Bitter
dc.creator.none.fl_str_mv Poulsen Hornum, Anabela
Camadro, Elsa Lucila
author Poulsen Hornum, Anabela
author_facet Poulsen Hornum, Anabela
Camadro, Elsa Lucila
author_role author
author2 Camadro, Elsa Lucila
author2_role author
dc.subject.none.fl_str_mv Solanum chacoense
Germplasm
Germplasm Banks
Germplasm Conservation
Potatoes
Germoplasma
Banco de Germoplasma
Conservación del Germoplasma
Papa
Papa Silvestre
topic Solanum chacoense
Germplasm
Germplasm Banks
Germplasm Conservation
Potatoes
Germoplasma
Banco de Germoplasma
Conservación del Germoplasma
Papa
Papa Silvestre
dc.description.none.fl_txt_mv Crop wild relatives are conserved in germplasm banks as original accessions or their ex situ regenerated progenies. The commonly used sexual regeneration method in wild potatoes (FAO 2013)—cultivation of 20–25 plants/accession (N), hand-pollination with pollen mixtures when a percentage of the plants are in bloom, and composition of the regenerated accession without controlling the number of seeds provided by each female progenitor—does not consider the action of internal reproductive barriers. However, wild potatoes—mostly diploid and obligate out-crossers—may present internal pre-zygotic, post-zygotic, or both types of complete or incomplete reproductive barriers, which can reduce the effective number of progenitors (Ne) in relation to N. To evaluate possible phenotypic and genetic changes during a sexual reproduction cycle, two regeneration protocols were followed with one accession of Solanum chacoense Bitter: the current one and the proposed by Camadro (2012). The original accession and the two regenerated populations were morphologically and molecularly characterized. Twenty-one morphological characters (quantitative and qualitative) were considered, and the data were subjected to univariate and multivariate analyses. Allele frequencies were calculated for six microsatellite markers (SSR) and compared among populations with a chi-square test (α = 0.05). Great morphological variability for morphological phenotypes and four of the six analyzed SSR, as well as changes in allele frequencies (including allele loss) were revealed within and among the three populations. Therefore, it is advisable to consider aspects of reproductive and population genetics in developing regeneration protocols to prevent or reduce the risks of genetic erosion in sexual regeneration cycles.
EEA Balcarce
Fil: Poulsen Hornum, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada; Argentina
Fil: Poulsen Hornum, Anabela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Unidad Integrada; Argentina
Fil: Poulsen Hornum, Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada; Argentina
Fil: Camadro, Elsa Lucila. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Unidad Integrada; Argentina
Fil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Crop wild relatives are conserved in germplasm banks as original accessions or their ex situ regenerated progenies. The commonly used sexual regeneration method in wild potatoes (FAO 2013)—cultivation of 20–25 plants/accession (N), hand-pollination with pollen mixtures when a percentage of the plants are in bloom, and composition of the regenerated accession without controlling the number of seeds provided by each female progenitor—does not consider the action of internal reproductive barriers. However, wild potatoes—mostly diploid and obligate out-crossers—may present internal pre-zygotic, post-zygotic, or both types of complete or incomplete reproductive barriers, which can reduce the effective number of progenitors (Ne) in relation to N. To evaluate possible phenotypic and genetic changes during a sexual reproduction cycle, two regeneration protocols were followed with one accession of Solanum chacoense Bitter: the current one and the proposed by Camadro (2012). The original accession and the two regenerated populations were morphologically and molecularly characterized. Twenty-one morphological characters (quantitative and qualitative) were considered, and the data were subjected to univariate and multivariate analyses. Allele frequencies were calculated for six microsatellite markers (SSR) and compared among populations with a chi-square test (α = 0.05). Great morphological variability for morphological phenotypes and four of the six analyzed SSR, as well as changes in allele frequencies (including allele loss) were revealed within and among the three populations. Therefore, it is advisable to consider aspects of reproductive and population genetics in developing regeneration protocols to prevent or reduce the risks of genetic erosion in sexual regeneration cycles.
publishDate 2024
dc.date.none.fl_str_mv 2024
2026-03-10T11:13:03Z
2026-03-10T11:13:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25407
https://link.springer.com/article/10.1007/s10722-023-01742-1
0925-9864 (print)
1573-5109 (online)
https://doi.org/10.1007/s10722-023-01742-1
url http://hdl.handle.net/20.500.12123/25407
https://link.springer.com/article/10.1007/s10722-023-01742-1
https://doi.org/10.1007/s10722-023-01742-1
identifier_str_mv 0925-9864 (print)
1573-5109 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNHFA-1106074/AR./Bases para la sostenibilidad de las cadenas de la papa y la batata.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Genetic Resources and Crop Evolution 71 : 2467-2484 (2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.977003

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