Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins

Autores
Kotsovolos, Nikolaos; Schnittger, Leonhard; Sibeko-Matjila, Kgomotso
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite’s rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa.
Instituto de Patobiología
Fil: Kotsovolos, Nikolaos. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; Sudáfrica
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Sibeko-Matjila, Kgomotso. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; Sudáfrica
Fuente
Computational Biology and Chemistry 120 (1): 108653. (February 2026)
Materia
Bioinformática
Theileria parva
Proteína Aglutinante
Enfermedades de los Animales
Bioinformatics
Binding Proteins
Animal Diseases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24800
Acceder
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spelling Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteinsKotsovolos, NikolaosSchnittger, LeonhardSibeko-Matjila, KgomotsoBioinformáticaTheileria parvaProteína AglutinanteEnfermedades de los AnimalesBioinformaticsBinding ProteinsAnimal DiseasesAs an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite’s rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa.Instituto de PatobiologíaFil: Kotsovolos, Nikolaos. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; SudáfricaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Sibeko-Matjila, Kgomotso. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; SudáfricaElsevier2025-12-29T16:04:35Z2025-12-29T16:04:35Z2026-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24800https://www.sciencedirect.com/science/article/pii/S14769271250031471476-92711476-928X (online)https://doi.org/10.1016/j.compbiolchem.2025.108653Computational Biology and Chemistry 120 (1): 108653. (February 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-01-08T10:41:01Zoai:localhost:20.500.12123/24800instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:41:01.571INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
spellingShingle Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
Kotsovolos, Nikolaos
Bioinformática
Theileria parva
Proteína Aglutinante
Enfermedades de los Animales
Bioinformatics
Binding Proteins
Animal Diseases
title_short Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title_full Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title_fullStr Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title_full_unstemmed Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title_sort Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
dc.creator.none.fl_str_mv Kotsovolos, Nikolaos
Schnittger, Leonhard
Sibeko-Matjila, Kgomotso
author Kotsovolos, Nikolaos
author_facet Kotsovolos, Nikolaos
Schnittger, Leonhard
Sibeko-Matjila, Kgomotso
author_role author
author2 Schnittger, Leonhard
Sibeko-Matjila, Kgomotso
author2_role author
author
dc.subject.none.fl_str_mv Bioinformática
Theileria parva
Proteína Aglutinante
Enfermedades de los Animales
Bioinformatics
Binding Proteins
Animal Diseases
topic Bioinformática
Theileria parva
Proteína Aglutinante
Enfermedades de los Animales
Bioinformatics
Binding Proteins
Animal Diseases
dc.description.none.fl_txt_mv As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite’s rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa.
Instituto de Patobiología
Fil: Kotsovolos, Nikolaos. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; Sudáfrica
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Sibeko-Matjila, Kgomotso. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; Sudáfrica
description As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite’s rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa.
publishDate 2025
dc.date.none.fl_str_mv 2025-12-29T16:04:35Z
2025-12-29T16:04:35Z
2026-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24800
https://www.sciencedirect.com/science/article/pii/S1476927125003147
1476-9271
1476-928X (online)
https://doi.org/10.1016/j.compbiolchem.2025.108653
url http://hdl.handle.net/20.500.12123/24800
https://www.sciencedirect.com/science/article/pii/S1476927125003147
https://doi.org/10.1016/j.compbiolchem.2025.108653
identifier_str_mv 1476-9271
1476-928X (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Computational Biology and Chemistry 120 (1): 108653. (February 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.25844

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