Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies d...

Autores
Obara, Isaiah; Nielsen, Morten; Jeschek, Marie; Nijhof, Ard; Mazzoni, Camila J.; Svitek, Nicholas; Steinaa, Lucilla; Awino, Elias; Olds, Cassandra; Jabbar, Ahmed; Clausen, Peter Henning; Bishop, Richard P.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There is strong evidence that the immunity induced by live vaccination for control of the protozoan parasite Theileria parva is mediated by class I MHC-restricted CD8+ T cells directed against the schizont stage of the parasite that infects bovine lymphocytes. The functional competency of class I MHC genes is dependent on the presence of codons specifying certain critical amino acid residues that line the peptide binding groove. Compared with European Bos taurus in which class I MHC allelic polymorphisms have been examined extensively, published data on class I MHC transcripts in African taurines in T. parva endemic areas is very limited. We utilized the multiplexing capabilities of 454 pyrosequencing to make an initial assessment of class I MHC allelic diversity in a population of Ankole cattle. We also typed a population of exotic Holstein cattle from an African ranch for class I MHC and investigated the extent, if any, that their peptide-binding motifs overlapped with those of Ankole cattle. We report the identification of 18 novel allelic sequences in Ankole cattle and provide evidence of positive selection for sequence diversity, including in residues that predominantly interact with peptides. In silico functional analysis resulted in peptide binding specificities that were largely distinct between the two breeds. We also demonstrate that CD8+ T cells derived from Ankole cattle that are seropositive for T. parva do not recognize vaccine candidate antigens originally identified in Holstein and Boran (Bos indicus) cattle breeds.
Fil: Obara, Isaiah. Freie Universität Berlin; Alemania
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Jeschek, Marie. Berlin Center for Genomics in Biodiversity Research; Alemania
Fil: Nijhof, Ard. Freie Universität Berlin; Alemania
Fil: Mazzoni, Camila J.. Berlin Center for Genomics in Biodiversity Research; Alemania. Evolutionary Genetics; Alemania
Fil: Svitek, Nicholas. International Livestock Research Institute; Kenia
Fil: Steinaa, Lucilla. International Livestock Research Institute; Kenia
Fil: Awino, Elias. International Livestock Research Institute; Kenia
Fil: Olds, Cassandra. International Livestock Research Institute; Kenia
Fil: Jabbar, Ahmed. Freie Universität Berlin; Alemania
Fil: Clausen, Peter Henning. Freie Universität Berlin; Alemania
Fil: Bishop, Richard P.. International Livestock Research Institute; Kenia
Materia
ANKOLE CATTLE
CD8+ T CELL EPITOPES
CLASS I MHC
PYROSEQUENCING
THEILERIA PARVA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51498
Acceder
id CONICETDig_e817f34dff2126a7f981215d2489fd4f
oai_identifier_str oai:ri.conicet.gov.ar:11336/51498
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clustersObara, IsaiahNielsen, MortenJeschek, MarieNijhof, ArdMazzoni, Camila J.Svitek, NicholasSteinaa, LucillaAwino, EliasOlds, CassandraJabbar, AhmedClausen, Peter HenningBishop, Richard P.ANKOLE CATTLECD8+ T CELL EPITOPESCLASS I MHCPYROSEQUENCINGTHEILERIA PARVAhttps://purl.org/becyt/ford/2.6https://purl.org/becyt/ford/2https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3There is strong evidence that the immunity induced by live vaccination for control of the protozoan parasite Theileria parva is mediated by class I MHC-restricted CD8+ T cells directed against the schizont stage of the parasite that infects bovine lymphocytes. The functional competency of class I MHC genes is dependent on the presence of codons specifying certain critical amino acid residues that line the peptide binding groove. Compared with European Bos taurus in which class I MHC allelic polymorphisms have been examined extensively, published data on class I MHC transcripts in African taurines in T. parva endemic areas is very limited. We utilized the multiplexing capabilities of 454 pyrosequencing to make an initial assessment of class I MHC allelic diversity in a population of Ankole cattle. We also typed a population of exotic Holstein cattle from an African ranch for class I MHC and investigated the extent, if any, that their peptide-binding motifs overlapped with those of Ankole cattle. We report the identification of 18 novel allelic sequences in Ankole cattle and provide evidence of positive selection for sequence diversity, including in residues that predominantly interact with peptides. In silico functional analysis resulted in peptide binding specificities that were largely distinct between the two breeds. We also demonstrate that CD8+ T cells derived from Ankole cattle that are seropositive for T. parva do not recognize vaccine candidate antigens originally identified in Holstein and Boran (Bos indicus) cattle breeds.Fil: Obara, Isaiah. Freie Universität Berlin; AlemaniaFil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Jeschek, Marie. Berlin Center for Genomics in Biodiversity Research; AlemaniaFil: Nijhof, Ard. Freie Universität Berlin; AlemaniaFil: Mazzoni, Camila J.. Berlin Center for Genomics in Biodiversity Research; Alemania. Evolutionary Genetics; AlemaniaFil: Svitek, Nicholas. International Livestock Research Institute; KeniaFil: Steinaa, Lucilla. International Livestock Research Institute; KeniaFil: Awino, Elias. International Livestock Research Institute; KeniaFil: Olds, Cassandra. International Livestock Research Institute; KeniaFil: Jabbar, Ahmed. Freie Universität Berlin; AlemaniaFil: Clausen, Peter Henning. Freie Universität Berlin; AlemaniaFil: Bishop, Richard P.. International Livestock Research Institute; KeniaSpringer2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51498Obara, Isaiah; Nielsen, Morten; Jeschek, Marie; Nijhof, Ard; Mazzoni, Camila J.; et al.; Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters; Springer; Immunogenetics; 68; 5; 5-2016; 339-3520093-7711CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00251-016-0902-5info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00251-016-0902-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:19:01Zoai:ri.conicet.gov.ar:11336/51498instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:19:02.231CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters
title Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters
spellingShingle Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters
Obara, Isaiah
ANKOLE CATTLE
CD8+ T CELL EPITOPES
CLASS I MHC
PYROSEQUENCING
THEILERIA PARVA
title_short Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters
title_full Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters
title_fullStr Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters
title_full_unstemmed Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters
title_sort Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters
dc.creator.none.fl_str_mv Obara, Isaiah
Nielsen, Morten
Jeschek, Marie
Nijhof, Ard
Mazzoni, Camila J.
Svitek, Nicholas
Steinaa, Lucilla
Awino, Elias
Olds, Cassandra
Jabbar, Ahmed
Clausen, Peter Henning
Bishop, Richard P.
author Obara, Isaiah
author_facet Obara, Isaiah
Nielsen, Morten
Jeschek, Marie
Nijhof, Ard
Mazzoni, Camila J.
Svitek, Nicholas
Steinaa, Lucilla
Awino, Elias
Olds, Cassandra
Jabbar, Ahmed
Clausen, Peter Henning
Bishop, Richard P.
author_role author
author2 Nielsen, Morten
Jeschek, Marie
Nijhof, Ard
Mazzoni, Camila J.
Svitek, Nicholas
Steinaa, Lucilla
Awino, Elias
Olds, Cassandra
Jabbar, Ahmed
Clausen, Peter Henning
Bishop, Richard P.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANKOLE CATTLE
CD8+ T CELL EPITOPES
CLASS I MHC
PYROSEQUENCING
THEILERIA PARVA
topic ANKOLE CATTLE
CD8+ T CELL EPITOPES
CLASS I MHC
PYROSEQUENCING
THEILERIA PARVA
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv There is strong evidence that the immunity induced by live vaccination for control of the protozoan parasite Theileria parva is mediated by class I MHC-restricted CD8+ T cells directed against the schizont stage of the parasite that infects bovine lymphocytes. The functional competency of class I MHC genes is dependent on the presence of codons specifying certain critical amino acid residues that line the peptide binding groove. Compared with European Bos taurus in which class I MHC allelic polymorphisms have been examined extensively, published data on class I MHC transcripts in African taurines in T. parva endemic areas is very limited. We utilized the multiplexing capabilities of 454 pyrosequencing to make an initial assessment of class I MHC allelic diversity in a population of Ankole cattle. We also typed a population of exotic Holstein cattle from an African ranch for class I MHC and investigated the extent, if any, that their peptide-binding motifs overlapped with those of Ankole cattle. We report the identification of 18 novel allelic sequences in Ankole cattle and provide evidence of positive selection for sequence diversity, including in residues that predominantly interact with peptides. In silico functional analysis resulted in peptide binding specificities that were largely distinct between the two breeds. We also demonstrate that CD8+ T cells derived from Ankole cattle that are seropositive for T. parva do not recognize vaccine candidate antigens originally identified in Holstein and Boran (Bos indicus) cattle breeds.
Fil: Obara, Isaiah. Freie Universität Berlin; Alemania
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Jeschek, Marie. Berlin Center for Genomics in Biodiversity Research; Alemania
Fil: Nijhof, Ard. Freie Universität Berlin; Alemania
Fil: Mazzoni, Camila J.. Berlin Center for Genomics in Biodiversity Research; Alemania. Evolutionary Genetics; Alemania
Fil: Svitek, Nicholas. International Livestock Research Institute; Kenia
Fil: Steinaa, Lucilla. International Livestock Research Institute; Kenia
Fil: Awino, Elias. International Livestock Research Institute; Kenia
Fil: Olds, Cassandra. International Livestock Research Institute; Kenia
Fil: Jabbar, Ahmed. Freie Universität Berlin; Alemania
Fil: Clausen, Peter Henning. Freie Universität Berlin; Alemania
Fil: Bishop, Richard P.. International Livestock Research Institute; Kenia
description There is strong evidence that the immunity induced by live vaccination for control of the protozoan parasite Theileria parva is mediated by class I MHC-restricted CD8+ T cells directed against the schizont stage of the parasite that infects bovine lymphocytes. The functional competency of class I MHC genes is dependent on the presence of codons specifying certain critical amino acid residues that line the peptide binding groove. Compared with European Bos taurus in which class I MHC allelic polymorphisms have been examined extensively, published data on class I MHC transcripts in African taurines in T. parva endemic areas is very limited. We utilized the multiplexing capabilities of 454 pyrosequencing to make an initial assessment of class I MHC allelic diversity in a population of Ankole cattle. We also typed a population of exotic Holstein cattle from an African ranch for class I MHC and investigated the extent, if any, that their peptide-binding motifs overlapped with those of Ankole cattle. We report the identification of 18 novel allelic sequences in Ankole cattle and provide evidence of positive selection for sequence diversity, including in residues that predominantly interact with peptides. In silico functional analysis resulted in peptide binding specificities that were largely distinct between the two breeds. We also demonstrate that CD8+ T cells derived from Ankole cattle that are seropositive for T. parva do not recognize vaccine candidate antigens originally identified in Holstein and Boran (Bos indicus) cattle breeds.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51498
Obara, Isaiah; Nielsen, Morten; Jeschek, Marie; Nijhof, Ard; Mazzoni, Camila J.; et al.; Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters; Springer; Immunogenetics; 68; 5; 5-2016; 339-352
0093-7711
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51498
identifier_str_mv Obara, Isaiah; Nielsen, Morten; Jeschek, Marie; Nijhof, Ard; Mazzoni, Camila J.; et al.; Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters; Springer; Immunogenetics; 68; 5; 5-2016; 339-352
0093-7711
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00251-016-0902-5
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00251-016-0902-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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