Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage

• Autores: Müller, Gabriela L.; Budde, Claudio Olaf; Lauxmann, Martín Alexander; Triassi, Agustina; Andreo, Carlos Santiago; Drincovich, María Fabiana; Lara, María Valeria
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Functional Plant Biology 40 (5) : 449-459. (2013)
• Materia: Tomate; Solanum Lycopersicum; Fisiología Postcosecha; Maduración en Postcosecha; Pared Celular; Metabolismo; Almacenamiento en Frío; Aptitud para la Conservación; Tomatoes; Postharvest Physiology; Postharvest Ripening; Cell Walls; Metabolism; Cold Storage; Keeping Quality; Metabolismo de la Pared Celular; Daño por Frío; Endotransglicosilasa/Hidrolasa; Xyloglucan β-xilosidasa; Cell Wall Metabolism; Chilling Injury; Endotransgylcosylase/Hydrolase; Xyloglucan β-xylosidase
• Institución: Instituto Nacional de Tecnología Agropecuaria

To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum (20°C). In this work, Micro-Tom tomatoes stored at 20°C (normal ripening) were compared with those stored at 15°C or 4°C (chilling injury inducer) for 7 days. In contrast to 4°C, storage at 15°C delayed ripening with the benefit of not enhancing oxidative metabolism and of enabling ripening upon being transferred to 20°C. The transcriptional expression profile of enzymes related to cell wall metabolism was compared at the three temperatures. Although endo-β-1,4-glucanase (Cel1), which is associated with fruit decay, was largely increased after removal from 4°C storage, its expression was not modified in fruits stored at 15°C. Enhanced transcriptional expression of xyloglucan endotransgylcosylase/hydrolases (XTHs) XTH1, –2, –10 and –11, and of two β-xylosidases (Xyl1–2) was detected in fruits stored at 15°C with respect to those at 20°C. Following 2 days at 20°C, these transcripts remained higher in fruits stored at 15°C and XHT3 and –9 also increased. Ethylene evolution was similar in fruits kept at 15°C and 20°C; thus, the changes in the transcript profile and fruit properties between these treatments may be under the control of factors other than ethylene.
EEA San Pedro
Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina.
Fil: Lauxmann, Martin A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Triassi, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Andreo, Carlos Santiago. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.