Extracting DNA of nematodes communities from Argentine Pampas agricultural soils

Autores
Mondino, Eduardo Ariel; Covacevich, Fernanda; Studdert, Guillermo; Pimentel, João Pedro; Berbara, Ricardo Luiz Louro
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.
Fil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidade Federal Rural do Rio de Janeiro. Instituto de Agronomia. Departamento de Solos. Laboratório de Matéria Orgânica e Biologia do Solo; Brasil
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Studdert, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Pimentel, João Pedro. Universidade Federal Rural do Rio de Janeiro. Departamento de Entomologia e Fitopatologia. Instituto de Biologia; Brasil
Fil: Berbara, Ricardo Luiz Louro Universidade Federal Rural do Rio de Janeiro. Instituto de Agronomia. Departamento de Solos. Laboratório de Matéria Orgânica e Biologia do Solo; Brasil
Fuente
Anais da Academia Brasileira de Ciências 87 (2) : 691-697. (2015)
Materia
Suelos Agrícolas
Nematoda
ADN
Agroecosistemas
Agroecosystems
DNA
Agricultural Soils
Región Pampeana
Cetyltrimethylammonium Bromide
CTAB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2360
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2360
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Extracting DNA of nematodes communities from Argentine Pampas agricultural soilsMondino, Eduardo ArielCovacevich, FernandaStuddert, GuillermoPimentel, João PedroBerbara, Ricardo Luiz LouroSuelos AgrícolasNematodaADNAgroecosistemasAgroecosystemsDNAAgricultural SoilsRegión PampeanaCetyltrimethylammonium BromideCTABWe examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.Fil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidade Federal Rural do Rio de Janeiro. Instituto de Agronomia. Departamento de Solos. Laboratório de Matéria Orgânica e Biologia do Solo; BrasilFil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Studdert, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Pimentel, João Pedro. Universidade Federal Rural do Rio de Janeiro. Departamento de Entomologia e Fitopatologia. Instituto de Biologia; BrasilFil: Berbara, Ricardo Luiz Louro Universidade Federal Rural do Rio de Janeiro. Instituto de Agronomia. Departamento de Solos. Laboratório de Matéria Orgânica e Biologia do Solo; Brasil2018-05-09T14:37:28Z2018-05-09T14:37:28Z2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2360http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-376520150002006910001-37651678-2690 (Online version)http://dx.doi.org/10.1590/0001-3765201520130110Anais da Academia Brasileira de Ciências 87 (2) : 691-697. (2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:18Zoai:localhost:20.500.12123/2360instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:18.935INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
spellingShingle Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
Mondino, Eduardo Ariel
Suelos Agrícolas
Nematoda
ADN
Agroecosistemas
Agroecosystems
DNA
Agricultural Soils
Región Pampeana
Cetyltrimethylammonium Bromide
CTAB
title_short Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title_full Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title_fullStr Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title_full_unstemmed Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
title_sort Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
dc.creator.none.fl_str_mv Mondino, Eduardo Ariel
Covacevich, Fernanda
Studdert, Guillermo
Pimentel, João Pedro
Berbara, Ricardo Luiz Louro
author Mondino, Eduardo Ariel
author_facet Mondino, Eduardo Ariel
Covacevich, Fernanda
Studdert, Guillermo
Pimentel, João Pedro
Berbara, Ricardo Luiz Louro
author_role author
author2 Covacevich, Fernanda
Studdert, Guillermo
Pimentel, João Pedro
Berbara, Ricardo Luiz Louro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Suelos Agrícolas
Nematoda
ADN
Agroecosistemas
Agroecosystems
DNA
Agricultural Soils
Región Pampeana
Cetyltrimethylammonium Bromide
CTAB
topic Suelos Agrícolas
Nematoda
ADN
Agroecosistemas
Agroecosystems
DNA
Agricultural Soils
Región Pampeana
Cetyltrimethylammonium Bromide
CTAB
dc.description.none.fl_txt_mv We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.
Fil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidade Federal Rural do Rio de Janeiro. Instituto de Agronomia. Departamento de Solos. Laboratório de Matéria Orgânica e Biologia do Solo; Brasil
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Studdert, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Pimentel, João Pedro. Universidade Federal Rural do Rio de Janeiro. Departamento de Entomologia e Fitopatologia. Instituto de Biologia; Brasil
Fil: Berbara, Ricardo Luiz Louro Universidade Federal Rural do Rio de Janeiro. Instituto de Agronomia. Departamento de Solos. Laboratório de Matéria Orgânica e Biologia do Solo; Brasil
description We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.
publishDate 2015
dc.date.none.fl_str_mv 2015
2018-05-09T14:37:28Z
2018-05-09T14:37:28Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2360
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691
0001-3765
1678-2690 (Online version)
http://dx.doi.org/10.1590/0001-3765201520130110
url http://hdl.handle.net/20.500.12123/2360
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691
http://dx.doi.org/10.1590/0001-3765201520130110
identifier_str_mv 0001-3765
1678-2690 (Online version)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências 87 (2) : 691-697. (2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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