Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis

Autores
Echeverria, Gustavo; Zumarraga, Martin Jose; Proaño‑Pérez, Freddy; Barceló Blasco, Francisco; Waard, Jacobus H. de
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although several brands of tuberculin purified protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the field and laboratory results showed significant differences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have influenced the immune reaction toward the different tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.
Instituto de Biotecnología
Fil: Echeverria, Gustavo. Universidad Central del Ecuador. Instituto de Investigación en Zoonosis-CIZ; Ecuador
Fil: Echeverria, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Echeverria, Gustavo. BioGENA. División Investigación y Desarrollo; Ecuador
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Proaño‑Pérez, Freddy. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia; Ecuador
Fil: Barceló Blasco, Francisco. Universidad de las Américas. Facultad de Ciencias de la Salud. One Health Research Group; Ecuador
Fil: Waard, Jacobus H. de. Universidad de las Américas. Facultad de Ciencias de la Salud. One Health Research Group; Ecuador
Fuente
Scientifc Reports 14 : 5155 (Published: 02 March 2024)
Materia
Tuberculin
Bovine Tuberculosis
Diagnosis
Tuberculina
Tuberculosis Bovina
Diagnóstico
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosisEcheverria, GustavoZumarraga, Martin JoseProaño‑Pérez, FreddyBarceló Blasco, FranciscoWaard, Jacobus H. deTuberculinBovine TuberculosisDiagnosisTuberculinaTuberculosis BovinaDiagnósticoAlthough several brands of tuberculin purified protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the field and laboratory results showed significant differences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have influenced the immune reaction toward the different tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.Instituto de BiotecnologíaFil: Echeverria, Gustavo. Universidad Central del Ecuador. Instituto de Investigación en Zoonosis-CIZ; EcuadorFil: Echeverria, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Echeverria, Gustavo. BioGENA. División Investigación y Desarrollo; EcuadorFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Proaño‑Pérez, Freddy. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia; EcuadorFil: Barceló Blasco, Francisco. Universidad de las Américas. Facultad de Ciencias de la Salud. One Health Research Group; EcuadorFil: Waard, Jacobus H. de. Universidad de las Américas. Facultad de Ciencias de la Salud. One Health Research Group; EcuadorSpringer Nature2024-06-24T10:27:20Z2024-06-24T10:27:20Z2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18233https://www.nature.com/articles/s41598-024-52089-12045-2322https://doi.org/10.1038/s41598-024-52089-1Scientifc Reports 14 : 5155 (Published: 02 March 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:27Zoai:localhost:20.500.12123/18233instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:27.578INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
spellingShingle Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
Echeverria, Gustavo
Tuberculin
Bovine Tuberculosis
Diagnosis
Tuberculina
Tuberculosis Bovina
Diagnóstico
title_short Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title_full Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title_fullStr Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title_full_unstemmed Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
title_sort Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
dc.creator.none.fl_str_mv Echeverria, Gustavo
Zumarraga, Martin Jose
Proaño‑Pérez, Freddy
Barceló Blasco, Francisco
Waard, Jacobus H. de
author Echeverria, Gustavo
author_facet Echeverria, Gustavo
Zumarraga, Martin Jose
Proaño‑Pérez, Freddy
Barceló Blasco, Francisco
Waard, Jacobus H. de
author_role author
author2 Zumarraga, Martin Jose
Proaño‑Pérez, Freddy
Barceló Blasco, Francisco
Waard, Jacobus H. de
author2_role author
author
author
author
dc.subject.none.fl_str_mv Tuberculin
Bovine Tuberculosis
Diagnosis
Tuberculina
Tuberculosis Bovina
Diagnóstico
topic Tuberculin
Bovine Tuberculosis
Diagnosis
Tuberculina
Tuberculosis Bovina
Diagnóstico
dc.description.none.fl_txt_mv Although several brands of tuberculin purified protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the field and laboratory results showed significant differences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have influenced the immune reaction toward the different tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.
Instituto de Biotecnología
Fil: Echeverria, Gustavo. Universidad Central del Ecuador. Instituto de Investigación en Zoonosis-CIZ; Ecuador
Fil: Echeverria, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Echeverria, Gustavo. BioGENA. División Investigación y Desarrollo; Ecuador
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Proaño‑Pérez, Freddy. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia; Ecuador
Fil: Barceló Blasco, Francisco. Universidad de las Américas. Facultad de Ciencias de la Salud. One Health Research Group; Ecuador
Fil: Waard, Jacobus H. de. Universidad de las Américas. Facultad de Ciencias de la Salud. One Health Research Group; Ecuador
description Although several brands of tuberculin purified protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the field and laboratory results showed significant differences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have influenced the immune reaction toward the different tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.
publishDate 2024
dc.date.none.fl_str_mv 2024-06-24T10:27:20Z
2024-06-24T10:27:20Z
2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18233
https://www.nature.com/articles/s41598-024-52089-1
2045-2322
https://doi.org/10.1038/s41598-024-52089-1
url http://hdl.handle.net/20.500.12123/18233
https://www.nature.com/articles/s41598-024-52089-1
https://doi.org/10.1038/s41598-024-52089-1
identifier_str_mv 2045-2322
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Scientifc Reports 14 : 5155 (Published: 02 March 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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