Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)

Autores
Badaracco, Alejandra; Matthijnssens, Jelle; Romero, Sandra Raquel; Heylen, Elisabeth; Zeller, Mark; Garaicoechea, Lorena Laura; Van Ranst, Marc; Parreño, Gladys Viviana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in all 4 members of the SAC, and so far, RVA has been detected from alpacas, llamas and guanacos. A total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in Jujuy, Argentina were collected and analyzed by ELISA to detect RVA antigen. Two samples (3%) were found to contain G8 RVA strains and one strain (RVA/Vicuña-wt/ARG/C75/2010/G8P[14]) was selected for further genome analyses, revealing the G8-P[14]-I2-R2-C2-M2-Ax-N2-T6-E3-Hx genotype constellation. Unfortunately, no sequence data could be obtained for NSP1 and NSP5. Except for the E3 NSP4 genotype, this partial genotype constellation is reminiscent to bovine RVA strains and bovine-like RVA strains isolated from sheep, guanaco, antelope and humans. This relationship was confirmed phylogenetically, providing further evidence of the widespread presence of this genotype constellation in animals belonging to the artiodactyls. In particular, a close phylogenetic relationship was found between C75 and guanaco RVA strain RVA/Guanaco-wt/ARG/Chubut/1999/G8P[14] for at least 5 gene segments, suggesting a partial conservation of the genotype constellation of RVA strains infecting different species of SACs, even though nowadays their natural habitats are not overlapping. The further monitoring of the sanitary health of wild newborn and juvenile vicuñas is essential to improve the management practices applied in their sustainable exploitation.
Instituto de Virología
Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Matthijnssens, Jelle. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica
Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; Argentina
Fil: Heylen, Elisabeth. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica
Fil: Zeller, Mark. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica
Fil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Van Ranst, Marc. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fuente
Veterinary Microbiology 161 (3–4) : 247-254 (January 2013)
Materia
Vicuña
Virus de los Animales
Rotavirus
Identificación
Anticuerpos
Vicunas
Animal Viruses
Identification
Antibodies
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4318
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4318
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)Badaracco, AlejandraMatthijnssens, JelleRomero, Sandra RaquelHeylen, ElisabethZeller, MarkGaraicoechea, Lorena LauraVan Ranst, MarcParreño, Gladys VivianaVicuñaVirus de los AnimalesRotavirusIdentificaciónAnticuerposVicunasAnimal VirusesIdentificationAntibodiesArgentinaThe wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in all 4 members of the SAC, and so far, RVA has been detected from alpacas, llamas and guanacos. A total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in Jujuy, Argentina were collected and analyzed by ELISA to detect RVA antigen. Two samples (3%) were found to contain G8 RVA strains and one strain (RVA/Vicuña-wt/ARG/C75/2010/G8P[14]) was selected for further genome analyses, revealing the G8-P[14]-I2-R2-C2-M2-Ax-N2-T6-E3-Hx genotype constellation. Unfortunately, no sequence data could be obtained for NSP1 and NSP5. Except for the E3 NSP4 genotype, this partial genotype constellation is reminiscent to bovine RVA strains and bovine-like RVA strains isolated from sheep, guanaco, antelope and humans. This relationship was confirmed phylogenetically, providing further evidence of the widespread presence of this genotype constellation in animals belonging to the artiodactyls. In particular, a close phylogenetic relationship was found between C75 and guanaco RVA strain RVA/Guanaco-wt/ARG/Chubut/1999/G8P[14] for at least 5 gene segments, suggesting a partial conservation of the genotype constellation of RVA strains infecting different species of SACs, even though nowadays their natural habitats are not overlapping. The further monitoring of the sanitary health of wild newborn and juvenile vicuñas is essential to improve the management practices applied in their sustainable exploitation.Instituto de VirologíaFil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Matthijnssens, Jelle. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; BélgicaFil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; ArgentinaFil: Heylen, Elisabeth. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; BélgicaFil: Zeller, Mark. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; BélgicaFil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Van Ranst, Marc. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; BélgicaFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaElsevier2019-01-23T14:59:02Z2019-01-23T14:59:02Z2013-01-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0378113512004208http://hdl.handle.net/20.500.12123/43180378-1135https://doi.org/10.1016/j.vetmic.2012.07.035Veterinary Microbiology 161 (3–4) : 247-254 (January 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:25Zoai:localhost:20.500.12123/4318instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:26.182INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
title Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
spellingShingle Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
Badaracco, Alejandra
Vicuña
Virus de los Animales
Rotavirus
Identificación
Anticuerpos
Vicunas
Animal Viruses
Identification
Antibodies
Argentina
title_short Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
title_full Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
title_fullStr Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
title_full_unstemmed Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
title_sort Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
dc.creator.none.fl_str_mv Badaracco, Alejandra
Matthijnssens, Jelle
Romero, Sandra Raquel
Heylen, Elisabeth
Zeller, Mark
Garaicoechea, Lorena Laura
Van Ranst, Marc
Parreño, Gladys Viviana
author Badaracco, Alejandra
author_facet Badaracco, Alejandra
Matthijnssens, Jelle
Romero, Sandra Raquel
Heylen, Elisabeth
Zeller, Mark
Garaicoechea, Lorena Laura
Van Ranst, Marc
Parreño, Gladys Viviana
author_role author
author2 Matthijnssens, Jelle
Romero, Sandra Raquel
Heylen, Elisabeth
Zeller, Mark
Garaicoechea, Lorena Laura
Van Ranst, Marc
Parreño, Gladys Viviana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Vicuña
Virus de los Animales
Rotavirus
Identificación
Anticuerpos
Vicunas
Animal Viruses
Identification
Antibodies
Argentina
topic Vicuña
Virus de los Animales
Rotavirus
Identificación
Anticuerpos
Vicunas
Animal Viruses
Identification
Antibodies
Argentina
dc.description.none.fl_txt_mv The wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in all 4 members of the SAC, and so far, RVA has been detected from alpacas, llamas and guanacos. A total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in Jujuy, Argentina were collected and analyzed by ELISA to detect RVA antigen. Two samples (3%) were found to contain G8 RVA strains and one strain (RVA/Vicuña-wt/ARG/C75/2010/G8P[14]) was selected for further genome analyses, revealing the G8-P[14]-I2-R2-C2-M2-Ax-N2-T6-E3-Hx genotype constellation. Unfortunately, no sequence data could be obtained for NSP1 and NSP5. Except for the E3 NSP4 genotype, this partial genotype constellation is reminiscent to bovine RVA strains and bovine-like RVA strains isolated from sheep, guanaco, antelope and humans. This relationship was confirmed phylogenetically, providing further evidence of the widespread presence of this genotype constellation in animals belonging to the artiodactyls. In particular, a close phylogenetic relationship was found between C75 and guanaco RVA strain RVA/Guanaco-wt/ARG/Chubut/1999/G8P[14] for at least 5 gene segments, suggesting a partial conservation of the genotype constellation of RVA strains infecting different species of SACs, even though nowadays their natural habitats are not overlapping. The further monitoring of the sanitary health of wild newborn and juvenile vicuñas is essential to improve the management practices applied in their sustainable exploitation.
Instituto de Virología
Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Matthijnssens, Jelle. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica
Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; Argentina
Fil: Heylen, Elisabeth. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica
Fil: Zeller, Mark. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica
Fil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Van Ranst, Marc. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
description The wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in all 4 members of the SAC, and so far, RVA has been detected from alpacas, llamas and guanacos. A total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in Jujuy, Argentina were collected and analyzed by ELISA to detect RVA antigen. Two samples (3%) were found to contain G8 RVA strains and one strain (RVA/Vicuña-wt/ARG/C75/2010/G8P[14]) was selected for further genome analyses, revealing the G8-P[14]-I2-R2-C2-M2-Ax-N2-T6-E3-Hx genotype constellation. Unfortunately, no sequence data could be obtained for NSP1 and NSP5. Except for the E3 NSP4 genotype, this partial genotype constellation is reminiscent to bovine RVA strains and bovine-like RVA strains isolated from sheep, guanaco, antelope and humans. This relationship was confirmed phylogenetically, providing further evidence of the widespread presence of this genotype constellation in animals belonging to the artiodactyls. In particular, a close phylogenetic relationship was found between C75 and guanaco RVA strain RVA/Guanaco-wt/ARG/Chubut/1999/G8P[14] for at least 5 gene segments, suggesting a partial conservation of the genotype constellation of RVA strains infecting different species of SACs, even though nowadays their natural habitats are not overlapping. The further monitoring of the sanitary health of wild newborn and juvenile vicuñas is essential to improve the management practices applied in their sustainable exploitation.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-25
2019-01-23T14:59:02Z
2019-01-23T14:59:02Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0378113512004208
http://hdl.handle.net/20.500.12123/4318
0378-1135
https://doi.org/10.1016/j.vetmic.2012.07.035
url https://www.sciencedirect.com/science/article/pii/S0378113512004208
http://hdl.handle.net/20.500.12123/4318
https://doi.org/10.1016/j.vetmic.2012.07.035
identifier_str_mv 0378-1135
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Microbiology 161 (3–4) : 247-254 (January 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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