Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way

Autores
Varela, Anabella; Ibañez, Verónica Noé; Alonso, Rodrigo; Zavallo, Diego; Asurmendi, Sebastian; Gomez Talquenca, Gonzalo Sebastián; Marfil, Carlos Federico; Berli, Federico Javier
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in different environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the effects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplified polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP differentiation between vineyards was selected and analyzed through reduced representation bisulfite sequencing. Twenty-nine differentially methylated regions between vineyards were identified and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the different phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent.
Instituto de Biotecnología
Fil: Varela, Anabella. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Varela, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ibañez, Verónica N. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ibañez, Verónica N. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Rodrigo. Bodega Catena Zapata; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez Talquenca, Gonzalo Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Marfil, Carlos Federico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berli, Federico Javier. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Plant Cell Reports 40 (1) : 111-125 (Enero 2021)
Materia
Epigenetics
Methylation
Phenotypic Plasticity
Vineyards
Epigenético
Metilación
Plasticidad Fenotípica
Vitis vinifera
Viña
Malbec
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9082
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9082
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent wayVarela, AnabellaIbañez, Verónica NoéAlonso, RodrigoZavallo, DiegoAsurmendi, SebastianGomez Talquenca, Gonzalo SebastiánMarfil, Carlos FedericoBerli, Federico JavierEpigeneticsMethylationPhenotypic PlasticityVineyardsEpigenéticoMetilaciónPlasticidad FenotípicaVitis viniferaViñaMalbecClonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in different environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the effects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplified polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP differentiation between vineyards was selected and analyzed through reduced representation bisulfite sequencing. Twenty-nine differentially methylated regions between vineyards were identified and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the different phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent.Instituto de BiotecnologíaFil: Varela, Anabella. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Varela, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ibañez, Verónica N. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Ibañez, Verónica N. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, Rodrigo. Bodega Catena Zapata; ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez Talquenca, Gonzalo Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Marfil, Carlos Federico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Berli, Federico Javier. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer Verlag2021-04-14T11:01:39Z2021-04-14T11:01:39Z2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9082https://link.springer.com/article/10.1007/s00299-020-02617-w1432-203Xhttps://doi.org/10.1007/s00299-020-02617-wPlant Cell Reports 40 (1) : 111-125 (Enero 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:10Zoai:localhost:20.500.12123/9082instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:11.215INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
title Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
spellingShingle Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
Varela, Anabella
Epigenetics
Methylation
Phenotypic Plasticity
Vineyards
Epigenético
Metilación
Plasticidad Fenotípica
Vitis vinifera
Viña
Malbec
title_short Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
title_full Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
title_fullStr Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
title_full_unstemmed Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
title_sort Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
dc.creator.none.fl_str_mv Varela, Anabella
Ibañez, Verónica Noé
Alonso, Rodrigo
Zavallo, Diego
Asurmendi, Sebastian
Gomez Talquenca, Gonzalo Sebastián
Marfil, Carlos Federico
Berli, Federico Javier
author Varela, Anabella
author_facet Varela, Anabella
Ibañez, Verónica Noé
Alonso, Rodrigo
Zavallo, Diego
Asurmendi, Sebastian
Gomez Talquenca, Gonzalo Sebastián
Marfil, Carlos Federico
Berli, Federico Javier
author_role author
author2 Ibañez, Verónica Noé
Alonso, Rodrigo
Zavallo, Diego
Asurmendi, Sebastian
Gomez Talquenca, Gonzalo Sebastián
Marfil, Carlos Federico
Berli, Federico Javier
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Epigenetics
Methylation
Phenotypic Plasticity
Vineyards
Epigenético
Metilación
Plasticidad Fenotípica
Vitis vinifera
Viña
Malbec
topic Epigenetics
Methylation
Phenotypic Plasticity
Vineyards
Epigenético
Metilación
Plasticidad Fenotípica
Vitis vinifera
Viña
Malbec
dc.description.none.fl_txt_mv Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in different environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the effects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplified polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP differentiation between vineyards was selected and analyzed through reduced representation bisulfite sequencing. Twenty-nine differentially methylated regions between vineyards were identified and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the different phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent.
Instituto de Biotecnología
Fil: Varela, Anabella. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Varela, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ibañez, Verónica N. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ibañez, Verónica N. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Rodrigo. Bodega Catena Zapata; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez Talquenca, Gonzalo Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Marfil, Carlos Federico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berli, Federico Javier. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in different environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the effects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplified polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP differentiation between vineyards was selected and analyzed through reduced representation bisulfite sequencing. Twenty-nine differentially methylated regions between vineyards were identified and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the different phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-14T11:01:39Z
2021-04-14T11:01:39Z
2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9082
https://link.springer.com/article/10.1007/s00299-020-02617-w
1432-203X
https://doi.org/10.1007/s00299-020-02617-w
url http://hdl.handle.net/20.500.12123/9082
https://link.springer.com/article/10.1007/s00299-020-02617-w
https://doi.org/10.1007/s00299-020-02617-w
identifier_str_mv 1432-203X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv Plant Cell Reports 40 (1) : 111-125 (Enero 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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