Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way

Autores
Varela, Anabella Mercedes; Ibañez, Verónica Noé; Alonso, Rodrigo; Zavallo, Diego; Asurmendi, Sebastian; Gómez Talquenca, Sebastián; Marfil, Carlos Federico; Berli, Federico Javier
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in diferent environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the efects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplifed polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP diferentiation between vineyards was selected and analyzed through reduced representation bisulfte sequencing. Twenty-nine diferentially methylated regions between vineyards were identifed and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the diferent phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent.
Fil: Varela, Anabella Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ibañez, Verónica Noé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Alonso, Rodrigo. No especifíca;
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Mendoza-san Juan. Estacion Experimental Agropecuaria Mendoza. Agencia de Extension Rural Lujan de Cuyo.; Argentina
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
DIFFERENTIALLY METHYLATED REGIONS (DMR)
EPIGENETICS
MSAP
PHENOTYPIC PLASTICITY
RRBS
VITIS VINIFERA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142414
Acceder
id CONICETDig_0608a63705bbe4986dd0d31cd6d1a7ea
oai_identifier_str oai:ri.conicet.gov.ar:11336/142414
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent wayVarela, Anabella MercedesIbañez, Verónica NoéAlonso, RodrigoZavallo, DiegoAsurmendi, SebastianGómez Talquenca, SebastiánMarfil, Carlos FedericoBerli, Federico JavierDIFFERENTIALLY METHYLATED REGIONS (DMR)EPIGENETICSMSAPPHENOTYPIC PLASTICITYRRBSVITIS VINIFERAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in diferent environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the efects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplifed polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP diferentiation between vineyards was selected and analyzed through reduced representation bisulfte sequencing. Twenty-nine diferentially methylated regions between vineyards were identifed and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the diferent phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent.Fil: Varela, Anabella Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Ibañez, Verónica Noé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Alonso, Rodrigo. No especifíca;Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Mendoza-san Juan. Estacion Experimental Agropecuaria Mendoza. Agencia de Extension Rural Lujan de Cuyo.; ArgentinaFil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaSpringer2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142414Varela, Anabella Mercedes; Ibañez, Verónica Noé; Alonso, Rodrigo; Zavallo, Diego; Asurmendi, Sebastian; et al.; Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way; Springer; Plant Cell Reports; 10-2020; 1-150721-7714CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-020-02617-winfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00299-020-02617-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:12Zoai:ri.conicet.gov.ar:11336/142414instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:13.262CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way
title Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way
spellingShingle Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way
Varela, Anabella Mercedes
DIFFERENTIALLY METHYLATED REGIONS (DMR)
EPIGENETICS
MSAP
PHENOTYPIC PLASTICITY
RRBS
VITIS VINIFERA
title_short Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way
title_full Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way
title_fullStr Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way
title_full_unstemmed Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way
title_sort Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way
dc.creator.none.fl_str_mv Varela, Anabella Mercedes
Ibañez, Verónica Noé
Alonso, Rodrigo
Zavallo, Diego
Asurmendi, Sebastian
Gómez Talquenca, Sebastián
Marfil, Carlos Federico
Berli, Federico Javier
author Varela, Anabella Mercedes
author_facet Varela, Anabella Mercedes
Ibañez, Verónica Noé
Alonso, Rodrigo
Zavallo, Diego
Asurmendi, Sebastian
Gómez Talquenca, Sebastián
Marfil, Carlos Federico
Berli, Federico Javier
author_role author
author2 Ibañez, Verónica Noé
Alonso, Rodrigo
Zavallo, Diego
Asurmendi, Sebastian
Gómez Talquenca, Sebastián
Marfil, Carlos Federico
Berli, Federico Javier
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIFFERENTIALLY METHYLATED REGIONS (DMR)
EPIGENETICS
MSAP
PHENOTYPIC PLASTICITY
RRBS
VITIS VINIFERA
topic DIFFERENTIALLY METHYLATED REGIONS (DMR)
EPIGENETICS
MSAP
PHENOTYPIC PLASTICITY
RRBS
VITIS VINIFERA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in diferent environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the efects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplifed polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP diferentiation between vineyards was selected and analyzed through reduced representation bisulfte sequencing. Twenty-nine diferentially methylated regions between vineyards were identifed and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the diferent phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent.
Fil: Varela, Anabella Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ibañez, Verónica Noé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Alonso, Rodrigo. No especifíca;
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Mendoza-san Juan. Estacion Experimental Agropecuaria Mendoza. Agencia de Extension Rural Lujan de Cuyo.; Argentina
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in diferent environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the efects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplifed polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP diferentiation between vineyards was selected and analyzed through reduced representation bisulfte sequencing. Twenty-nine diferentially methylated regions between vineyards were identifed and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the diferent phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent.
publishDate 2020
dc.date.none.fl_str_mv 2020-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142414
Varela, Anabella Mercedes; Ibañez, Verónica Noé; Alonso, Rodrigo; Zavallo, Diego; Asurmendi, Sebastian; et al.; Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way; Springer; Plant Cell Reports; 10-2020; 1-15
0721-7714
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142414
identifier_str_mv Varela, Anabella Mercedes; Ibañez, Verónica Noé; Alonso, Rodrigo; Zavallo, Diego; Asurmendi, Sebastian; et al.; Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone-dependent way; Springer; Plant Cell Reports; 10-2020; 1-15
0721-7714
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-020-02617-w
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00299-020-02617-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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