Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea

Autores
Bello, Fernando; Montironi, Ivana Dalila; Medina, María Belén; Munitz, Martín Sebastián; Ferreira, Flavia Vanina; Williman, Celia; Vazquez, Daniel Eduardo; Cariddi, Laura Noelia; Musumeci, Matías Alejandro
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Botrytis cinerea, the causal agent of the gray mold, is a filamentous fungus that infects blueberries and can cause important production losses in postharvest storage. Considering that the use of synthetic fungicides is not allowed on blueberries in postharvest conditions, alternative and natural strategies are needed to control gray mold. The objective of this work was to evaluate the capability of volatile organic compounds (VOCs) produced by Trichoderma atroviride IC-11 to control B. cinerea growth in blueberries after harvest.These VOCs inhibited almost completely B. cinerea growth in vitro. The most abundant volatile compound was 6-pentyl-α-pyrone (6PP). In vitro assays with pure 6PP confirmed its antifungal activity. The incidence of gray mold was evaluated in blueberries inoculated with B. cinerea and exposed to volatiles of T. atroviride IC-11. Gray mold incidence among those stored in air at 20 °C for 14 days was 100%, while the incidence among the volatile-treated fruit was 17%. Gray mold incidence among those stored in air at 4 °C for 31 days was 82%, while the incidence among the volatile-treated fruit was 11%. T. atroviride IC-11 VOCs inhibited mycelial growth and conidia germination of B. cinerea. The binding of VOCs to the surface of hyphae caused their vacuolation and deterioration. Selective cytotoxicity of 6PP on B. cinerea was observed but not on human intestinal cells at specific concentrations that controlled gray mold. The postharvest mycofumigation of blueberries with T. atroviride IC-11 VOCs is a promising approach to protect these fruits from gray mold.
EEA Concordia
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Montironi, Ivana Dalila. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina
Fil: Medina, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Medina, María Belén. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Munitz, Martín Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Munitz, Martín Sebastián. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Williman, Celia. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina.
Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fuente
Food Microbiology : 104040 (Available online 14 April 2022)
Materia
Arándano
Fumigación
Trichoderma
Botrytis cinerea
Podredumbres
Control de Enfermedades
Vaccinium corymbosum
Blueberries
Fumigation
Rots
Diseases Control
Trichoderma atroviride
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11677
Acceder
id INTADig_9dbc631dff5b87fd139721113a754ac8
oai_identifier_str oai:localhost:20.500.12123/11677
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinereaBello, FernandoMontironi, Ivana DalilaMedina, María BelénMunitz, Martín SebastiánFerreira, Flavia VaninaWilliman, CeliaVazquez, Daniel EduardoCariddi, Laura NoeliaMusumeci, Matías AlejandroArándanoFumigaciónTrichodermaBotrytis cinereaPodredumbresControl de EnfermedadesVaccinium corymbosumBlueberriesFumigationRotsDiseases ControlTrichoderma atrovirideBotrytis cinerea, the causal agent of the gray mold, is a filamentous fungus that infects blueberries and can cause important production losses in postharvest storage. Considering that the use of synthetic fungicides is not allowed on blueberries in postharvest conditions, alternative and natural strategies are needed to control gray mold. The objective of this work was to evaluate the capability of volatile organic compounds (VOCs) produced by Trichoderma atroviride IC-11 to control B. cinerea growth in blueberries after harvest.These VOCs inhibited almost completely B. cinerea growth in vitro. The most abundant volatile compound was 6-pentyl-α-pyrone (6PP). In vitro assays with pure 6PP confirmed its antifungal activity. The incidence of gray mold was evaluated in blueberries inoculated with B. cinerea and exposed to volatiles of T. atroviride IC-11. Gray mold incidence among those stored in air at 20 °C for 14 days was 100%, while the incidence among the volatile-treated fruit was 17%. Gray mold incidence among those stored in air at 4 °C for 31 days was 82%, while the incidence among the volatile-treated fruit was 11%. T. atroviride IC-11 VOCs inhibited mycelial growth and conidia germination of B. cinerea. The binding of VOCs to the surface of hyphae caused their vacuolation and deterioration. Selective cytotoxicity of 6PP on B. cinerea was observed but not on human intestinal cells at specific concentrations that controlled gray mold. The postharvest mycofumigation of blueberries with T. atroviride IC-11 VOCs is a promising approach to protect these fruits from gray mold.EEA ConcordiaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Montironi, Ivana Dalila. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; ArgentinaFil: Medina, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; ArgentinaFil: Medina, María Belén. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Munitz, Martín Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; ArgentinaFil: Munitz, Martín Sebastián. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; ArgentinaFil: Williman, Celia. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina.Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; ArgentinaFil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2023-04-192022-04-19T12:55:47Z2022-04-19T12:55:47Z2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11677https://www.sciencedirect.com/science/article/pii/S07400020220006480740-0020https://doi.org/10.1016/j.fm.2022.104040Food Microbiology : 104040 (Available online 14 April 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:32Zoai:localhost:20.500.12123/11677instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:32.562INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea
title Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea
spellingShingle Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea
Bello, Fernando
Arándano
Fumigación
Trichoderma
Botrytis cinerea
Podredumbres
Control de Enfermedades
Vaccinium corymbosum
Blueberries
Fumigation
Rots
Diseases Control
Trichoderma atroviride
title_short Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea
title_full Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea
title_fullStr Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea
title_full_unstemmed Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea
title_sort Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea
dc.creator.none.fl_str_mv Bello, Fernando
Montironi, Ivana Dalila
Medina, María Belén
Munitz, Martín Sebastián
Ferreira, Flavia Vanina
Williman, Celia
Vazquez, Daniel Eduardo
Cariddi, Laura Noelia
Musumeci, Matías Alejandro
author Bello, Fernando
author_facet Bello, Fernando
Montironi, Ivana Dalila
Medina, María Belén
Munitz, Martín Sebastián
Ferreira, Flavia Vanina
Williman, Celia
Vazquez, Daniel Eduardo
Cariddi, Laura Noelia
Musumeci, Matías Alejandro
author_role author
author2 Montironi, Ivana Dalila
Medina, María Belén
Munitz, Martín Sebastián
Ferreira, Flavia Vanina
Williman, Celia
Vazquez, Daniel Eduardo
Cariddi, Laura Noelia
Musumeci, Matías Alejandro
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arándano
Fumigación
Trichoderma
Botrytis cinerea
Podredumbres
Control de Enfermedades
Vaccinium corymbosum
Blueberries
Fumigation
Rots
Diseases Control
Trichoderma atroviride
topic Arándano
Fumigación
Trichoderma
Botrytis cinerea
Podredumbres
Control de Enfermedades
Vaccinium corymbosum
Blueberries
Fumigation
Rots
Diseases Control
Trichoderma atroviride
dc.description.none.fl_txt_mv Botrytis cinerea, the causal agent of the gray mold, is a filamentous fungus that infects blueberries and can cause important production losses in postharvest storage. Considering that the use of synthetic fungicides is not allowed on blueberries in postharvest conditions, alternative and natural strategies are needed to control gray mold. The objective of this work was to evaluate the capability of volatile organic compounds (VOCs) produced by Trichoderma atroviride IC-11 to control B. cinerea growth in blueberries after harvest.These VOCs inhibited almost completely B. cinerea growth in vitro. The most abundant volatile compound was 6-pentyl-α-pyrone (6PP). In vitro assays with pure 6PP confirmed its antifungal activity. The incidence of gray mold was evaluated in blueberries inoculated with B. cinerea and exposed to volatiles of T. atroviride IC-11. Gray mold incidence among those stored in air at 20 °C for 14 days was 100%, while the incidence among the volatile-treated fruit was 17%. Gray mold incidence among those stored in air at 4 °C for 31 days was 82%, while the incidence among the volatile-treated fruit was 11%. T. atroviride IC-11 VOCs inhibited mycelial growth and conidia germination of B. cinerea. The binding of VOCs to the surface of hyphae caused their vacuolation and deterioration. Selective cytotoxicity of 6PP on B. cinerea was observed but not on human intestinal cells at specific concentrations that controlled gray mold. The postharvest mycofumigation of blueberries with T. atroviride IC-11 VOCs is a promising approach to protect these fruits from gray mold.
EEA Concordia
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Montironi, Ivana Dalila. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina
Fil: Medina, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Medina, María Belén. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Munitz, Martín Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Munitz, Martín Sebastián. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Williman, Celia. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina.
Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
description Botrytis cinerea, the causal agent of the gray mold, is a filamentous fungus that infects blueberries and can cause important production losses in postharvest storage. Considering that the use of synthetic fungicides is not allowed on blueberries in postharvest conditions, alternative and natural strategies are needed to control gray mold. The objective of this work was to evaluate the capability of volatile organic compounds (VOCs) produced by Trichoderma atroviride IC-11 to control B. cinerea growth in blueberries after harvest.These VOCs inhibited almost completely B. cinerea growth in vitro. The most abundant volatile compound was 6-pentyl-α-pyrone (6PP). In vitro assays with pure 6PP confirmed its antifungal activity. The incidence of gray mold was evaluated in blueberries inoculated with B. cinerea and exposed to volatiles of T. atroviride IC-11. Gray mold incidence among those stored in air at 20 °C for 14 days was 100%, while the incidence among the volatile-treated fruit was 17%. Gray mold incidence among those stored in air at 4 °C for 31 days was 82%, while the incidence among the volatile-treated fruit was 11%. T. atroviride IC-11 VOCs inhibited mycelial growth and conidia germination of B. cinerea. The binding of VOCs to the surface of hyphae caused their vacuolation and deterioration. Selective cytotoxicity of 6PP on B. cinerea was observed but not on human intestinal cells at specific concentrations that controlled gray mold. The postharvest mycofumigation of blueberries with T. atroviride IC-11 VOCs is a promising approach to protect these fruits from gray mold.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-19T12:55:47Z
2022-04-19T12:55:47Z
2022-04
info:eu-repo/date/embargoEnd/2023-04-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11677
https://www.sciencedirect.com/science/article/pii/S0740002022000648
0740-0020
https://doi.org/10.1016/j.fm.2022.104040
url http://hdl.handle.net/20.500.12123/11677
https://www.sciencedirect.com/science/article/pii/S0740002022000648
https://doi.org/10.1016/j.fm.2022.104040
identifier_str_mv 0740-0020
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Food Microbiology : 104040 (Available online 14 April 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619164096921600
score 12.559606

Publicaciones similares