Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata

Autores
Chacón, Florencia Isabel; Sineli, Pedro Eugenio; Guiñazú, Santiago Bernabé; Alvarez, Fátima Micaela; Poehlein, Anja; Daniel, Rolf; Dib, Julian Rafael
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Blueberry production in Argentina faces economic losses caused mainly by fungal pathogens such as Botrytis cinerea and Alternaria alternata. Synthetic fungicides are expected to be discontinued in favor of a more sustainable agriculture, making indigenous yeast, which protects the plants, a promising alternative. This study explored the cultivable portion of blueberry fruits and flowers epiphytic yeast community to identify yeasts capable of protecting fruits against the major phytopathogens affecting this crop. Native yeasts were isolated and identified based on the PCR-amplified and sequenced D1/D2 domains of the 26S rRNA gene. Members of the genera: Rhodotorula, Hanseniaspora, Naganishia, Moesziomyces, Rhizosphaera, Metschnikowia, Clavispora, Pallidophorina, Hannaella, Aureobasidium, Sporobolomyces, Filobasidium, and Cystobasidium were identified. Subsequently, antagonism against B. cinerea and A. alternata was assessed in vitro and in vivo. Finally, potential modes of action such as killer phenotype, siderophore production and formation of volatile organic compounds (VOCs) were also evaluated. Among 38 yeast strains identified, the strain Aureobasidium leucospermi LAF2 stood out in vitro and in vivo with respect to protect blueberry fruits against B. cinerea by 58.33 % and A. alternata by 67.78 % of biocontrol efficiency at 25 ◦C. It also proved the ability to produce hydroxamate-type siderophores and VOCs capable of inhibiting B. cinerea growth by 48.19 %. These results suggest that fruits and flowers epiphytic microbial communities are a reservoir of potential biological control agents. A. leucospermi LAF2 is a strong candidate for a sustainable alternative to synthetic fungicides in blueberry production.
Fil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Guiñazú, Santiago Bernabé. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alvarez, Fátima Micaela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Poehlein, Anja. Universität Göttingen; Alemania
Fil: Daniel, Rolf. Universität Göttingen; Alemania
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Materia
BLUEBERRY
BOTRYTIS CINEREA
ALTERNARIA ALTERNATA
BIOLOGICAL CONTROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272002
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272002
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternataChacón, Florencia IsabelSineli, Pedro EugenioGuiñazú, Santiago BernabéAlvarez, Fátima MicaelaPoehlein, AnjaDaniel, RolfDib, Julian RafaelBLUEBERRYBOTRYTIS CINEREAALTERNARIA ALTERNATABIOLOGICAL CONTROLhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Blueberry production in Argentina faces economic losses caused mainly by fungal pathogens such as Botrytis cinerea and Alternaria alternata. Synthetic fungicides are expected to be discontinued in favor of a more sustainable agriculture, making indigenous yeast, which protects the plants, a promising alternative. This study explored the cultivable portion of blueberry fruits and flowers epiphytic yeast community to identify yeasts capable of protecting fruits against the major phytopathogens affecting this crop. Native yeasts were isolated and identified based on the PCR-amplified and sequenced D1/D2 domains of the 26S rRNA gene. Members of the genera: Rhodotorula, Hanseniaspora, Naganishia, Moesziomyces, Rhizosphaera, Metschnikowia, Clavispora, Pallidophorina, Hannaella, Aureobasidium, Sporobolomyces, Filobasidium, and Cystobasidium were identified. Subsequently, antagonism against B. cinerea and A. alternata was assessed in vitro and in vivo. Finally, potential modes of action such as killer phenotype, siderophore production and formation of volatile organic compounds (VOCs) were also evaluated. Among 38 yeast strains identified, the strain Aureobasidium leucospermi LAF2 stood out in vitro and in vivo with respect to protect blueberry fruits against B. cinerea by 58.33 % and A. alternata by 67.78 % of biocontrol efficiency at 25 ◦C. It also proved the ability to produce hydroxamate-type siderophores and VOCs capable of inhibiting B. cinerea growth by 48.19 %. These results suggest that fruits and flowers epiphytic microbial communities are a reservoir of potential biological control agents. A. leucospermi LAF2 is a strong candidate for a sustainable alternative to synthetic fungicides in blueberry production.Fil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Guiñazú, Santiago Bernabé. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Alvarez, Fátima Micaela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Poehlein, Anja. Universität Göttingen; AlemaniaFil: Daniel, Rolf. Universität Göttingen; AlemaniaFil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaAcademic Press Inc Elsevier Science2025-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272002Chacón, Florencia Isabel; Sineli, Pedro Eugenio; Guiñazú, Santiago Bernabé; Alvarez, Fátima Micaela; Poehlein, Anja; et al.; Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata; Academic Press Inc Elsevier Science; Biological Control; 209; 10-2025; 1-111049-9644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S104996442500180Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2025.105870info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:35:08Zoai:ri.conicet.gov.ar:11336/272002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:35:08.939CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
title Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
spellingShingle Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
Chacón, Florencia Isabel
BLUEBERRY
BOTRYTIS CINEREA
ALTERNARIA ALTERNATA
BIOLOGICAL CONTROL
title_short Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
title_full Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
title_fullStr Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
title_full_unstemmed Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
title_sort Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
dc.creator.none.fl_str_mv Chacón, Florencia Isabel
Sineli, Pedro Eugenio
Guiñazú, Santiago Bernabé
Alvarez, Fátima Micaela
Poehlein, Anja
Daniel, Rolf
Dib, Julian Rafael
author Chacón, Florencia Isabel
author_facet Chacón, Florencia Isabel
Sineli, Pedro Eugenio
Guiñazú, Santiago Bernabé
Alvarez, Fátima Micaela
Poehlein, Anja
Daniel, Rolf
Dib, Julian Rafael
author_role author
author2 Sineli, Pedro Eugenio
Guiñazú, Santiago Bernabé
Alvarez, Fátima Micaela
Poehlein, Anja
Daniel, Rolf
Dib, Julian Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BLUEBERRY
BOTRYTIS CINEREA
ALTERNARIA ALTERNATA
BIOLOGICAL CONTROL
topic BLUEBERRY
BOTRYTIS CINEREA
ALTERNARIA ALTERNATA
BIOLOGICAL CONTROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Blueberry production in Argentina faces economic losses caused mainly by fungal pathogens such as Botrytis cinerea and Alternaria alternata. Synthetic fungicides are expected to be discontinued in favor of a more sustainable agriculture, making indigenous yeast, which protects the plants, a promising alternative. This study explored the cultivable portion of blueberry fruits and flowers epiphytic yeast community to identify yeasts capable of protecting fruits against the major phytopathogens affecting this crop. Native yeasts were isolated and identified based on the PCR-amplified and sequenced D1/D2 domains of the 26S rRNA gene. Members of the genera: Rhodotorula, Hanseniaspora, Naganishia, Moesziomyces, Rhizosphaera, Metschnikowia, Clavispora, Pallidophorina, Hannaella, Aureobasidium, Sporobolomyces, Filobasidium, and Cystobasidium were identified. Subsequently, antagonism against B. cinerea and A. alternata was assessed in vitro and in vivo. Finally, potential modes of action such as killer phenotype, siderophore production and formation of volatile organic compounds (VOCs) were also evaluated. Among 38 yeast strains identified, the strain Aureobasidium leucospermi LAF2 stood out in vitro and in vivo with respect to protect blueberry fruits against B. cinerea by 58.33 % and A. alternata by 67.78 % of biocontrol efficiency at 25 ◦C. It also proved the ability to produce hydroxamate-type siderophores and VOCs capable of inhibiting B. cinerea growth by 48.19 %. These results suggest that fruits and flowers epiphytic microbial communities are a reservoir of potential biological control agents. A. leucospermi LAF2 is a strong candidate for a sustainable alternative to synthetic fungicides in blueberry production.
Fil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Guiñazú, Santiago Bernabé. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alvarez, Fátima Micaela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Poehlein, Anja. Universität Göttingen; Alemania
Fil: Daniel, Rolf. Universität Göttingen; Alemania
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
description Blueberry production in Argentina faces economic losses caused mainly by fungal pathogens such as Botrytis cinerea and Alternaria alternata. Synthetic fungicides are expected to be discontinued in favor of a more sustainable agriculture, making indigenous yeast, which protects the plants, a promising alternative. This study explored the cultivable portion of blueberry fruits and flowers epiphytic yeast community to identify yeasts capable of protecting fruits against the major phytopathogens affecting this crop. Native yeasts were isolated and identified based on the PCR-amplified and sequenced D1/D2 domains of the 26S rRNA gene. Members of the genera: Rhodotorula, Hanseniaspora, Naganishia, Moesziomyces, Rhizosphaera, Metschnikowia, Clavispora, Pallidophorina, Hannaella, Aureobasidium, Sporobolomyces, Filobasidium, and Cystobasidium were identified. Subsequently, antagonism against B. cinerea and A. alternata was assessed in vitro and in vivo. Finally, potential modes of action such as killer phenotype, siderophore production and formation of volatile organic compounds (VOCs) were also evaluated. Among 38 yeast strains identified, the strain Aureobasidium leucospermi LAF2 stood out in vitro and in vivo with respect to protect blueberry fruits against B. cinerea by 58.33 % and A. alternata by 67.78 % of biocontrol efficiency at 25 ◦C. It also proved the ability to produce hydroxamate-type siderophores and VOCs capable of inhibiting B. cinerea growth by 48.19 %. These results suggest that fruits and flowers epiphytic microbial communities are a reservoir of potential biological control agents. A. leucospermi LAF2 is a strong candidate for a sustainable alternative to synthetic fungicides in blueberry production.
publishDate 2025
dc.date.none.fl_str_mv 2025-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272002
Chacón, Florencia Isabel; Sineli, Pedro Eugenio; Guiñazú, Santiago Bernabé; Alvarez, Fátima Micaela; Poehlein, Anja; et al.; Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata; Academic Press Inc Elsevier Science; Biological Control; 209; 10-2025; 1-11
1049-9644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272002
identifier_str_mv Chacón, Florencia Isabel; Sineli, Pedro Eugenio; Guiñazú, Santiago Bernabé; Alvarez, Fátima Micaela; Poehlein, Anja; et al.; Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata; Academic Press Inc Elsevier Science; Biological Control; 209; 10-2025; 1-11
1049-9644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S104996442500180X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2025.105870
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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