Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata
- Autores
- Chacón, Florencia Isabel; Sineli, Pedro Eugenio; Guiñazú, Santiago Bernabé; Alvarez, Fátima Micaela; Poehlein, Anja; Daniel, Rolf; Dib, Julian Rafael
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Blueberry production in Argentina faces economic losses caused mainly by fungal pathogens such as Botrytis cinerea and Alternaria alternata. Synthetic fungicides are expected to be discontinued in favor of a more sustainable agriculture, making indigenous yeast, which protects the plants, a promising alternative. This study explored the cultivable portion of blueberry fruits and flowers epiphytic yeast community to identify yeasts capable of protecting fruits against the major phytopathogens affecting this crop. Native yeasts were isolated and identified based on the PCR-amplified and sequenced D1/D2 domains of the 26S rRNA gene. Members of the genera: Rhodotorula, Hanseniaspora, Naganishia, Moesziomyces, Rhizosphaera, Metschnikowia, Clavispora, Pallidophorina, Hannaella, Aureobasidium, Sporobolomyces, Filobasidium, and Cystobasidium were identified. Subsequently, antagonism against B. cinerea and A. alternata was assessed in vitro and in vivo. Finally, potential modes of action such as killer phenotype, siderophore production and formation of volatile organic compounds (VOCs) were also evaluated. Among 38 yeast strains identified, the strain Aureobasidium leucospermi LAF2 stood out in vitro and in vivo with respect to protect blueberry fruits against B. cinerea by 58.33 % and A. alternata by 67.78 % of biocontrol efficiency at 25 ◦C. It also proved the ability to produce hydroxamate-type siderophores and VOCs capable of inhibiting B. cinerea growth by 48.19 %. These results suggest that fruits and flowers epiphytic microbial communities are a reservoir of potential biological control agents. A. leucospermi LAF2 is a strong candidate for a sustainable alternative to synthetic fungicides in blueberry production.
Fil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Guiñazú, Santiago Bernabé. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alvarez, Fátima Micaela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Poehlein, Anja. Universität Göttingen; Alemania
Fil: Daniel, Rolf. Universität Göttingen; Alemania
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina - Materia
-
BLUEBERRY
BOTRYTIS CINEREA
ALTERNARIA ALTERNATA
BIOLOGICAL CONTROL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/272002
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/272002 |
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Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternataChacón, Florencia IsabelSineli, Pedro EugenioGuiñazú, Santiago BernabéAlvarez, Fátima MicaelaPoehlein, AnjaDaniel, RolfDib, Julian RafaelBLUEBERRYBOTRYTIS CINEREAALTERNARIA ALTERNATABIOLOGICAL CONTROLhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Blueberry production in Argentina faces economic losses caused mainly by fungal pathogens such as Botrytis cinerea and Alternaria alternata. Synthetic fungicides are expected to be discontinued in favor of a more sustainable agriculture, making indigenous yeast, which protects the plants, a promising alternative. This study explored the cultivable portion of blueberry fruits and flowers epiphytic yeast community to identify yeasts capable of protecting fruits against the major phytopathogens affecting this crop. Native yeasts were isolated and identified based on the PCR-amplified and sequenced D1/D2 domains of the 26S rRNA gene. Members of the genera: Rhodotorula, Hanseniaspora, Naganishia, Moesziomyces, Rhizosphaera, Metschnikowia, Clavispora, Pallidophorina, Hannaella, Aureobasidium, Sporobolomyces, Filobasidium, and Cystobasidium were identified. Subsequently, antagonism against B. cinerea and A. alternata was assessed in vitro and in vivo. Finally, potential modes of action such as killer phenotype, siderophore production and formation of volatile organic compounds (VOCs) were also evaluated. Among 38 yeast strains identified, the strain Aureobasidium leucospermi LAF2 stood out in vitro and in vivo with respect to protect blueberry fruits against B. cinerea by 58.33 % and A. alternata by 67.78 % of biocontrol efficiency at 25 ◦C. It also proved the ability to produce hydroxamate-type siderophores and VOCs capable of inhibiting B. cinerea growth by 48.19 %. These results suggest that fruits and flowers epiphytic microbial communities are a reservoir of potential biological control agents. A. leucospermi LAF2 is a strong candidate for a sustainable alternative to synthetic fungicides in blueberry production.Fil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Guiñazú, Santiago Bernabé. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Alvarez, Fátima Micaela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Poehlein, Anja. Universität Göttingen; AlemaniaFil: Daniel, Rolf. Universität Göttingen; AlemaniaFil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaAcademic Press Inc Elsevier Science2025-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272002Chacón, Florencia Isabel; Sineli, Pedro Eugenio; Guiñazú, Santiago Bernabé; Alvarez, Fátima Micaela; Poehlein, Anja; et al.; Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata; Academic Press Inc Elsevier Science; Biological Control; 209; 10-2025; 1-111049-9644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S104996442500180Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2025.105870info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:09Zoai:ri.conicet.gov.ar:11336/272002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:10.272CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata |
title |
Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata |
spellingShingle |
Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata Chacón, Florencia Isabel BLUEBERRY BOTRYTIS CINEREA ALTERNARIA ALTERNATA BIOLOGICAL CONTROL |
title_short |
Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata |
title_full |
Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata |
title_fullStr |
Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata |
title_full_unstemmed |
Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata |
title_sort |
Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata |
dc.creator.none.fl_str_mv |
Chacón, Florencia Isabel Sineli, Pedro Eugenio Guiñazú, Santiago Bernabé Alvarez, Fátima Micaela Poehlein, Anja Daniel, Rolf Dib, Julian Rafael |
author |
Chacón, Florencia Isabel |
author_facet |
Chacón, Florencia Isabel Sineli, Pedro Eugenio Guiñazú, Santiago Bernabé Alvarez, Fátima Micaela Poehlein, Anja Daniel, Rolf Dib, Julian Rafael |
author_role |
author |
author2 |
Sineli, Pedro Eugenio Guiñazú, Santiago Bernabé Alvarez, Fátima Micaela Poehlein, Anja Daniel, Rolf Dib, Julian Rafael |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
BLUEBERRY BOTRYTIS CINEREA ALTERNARIA ALTERNATA BIOLOGICAL CONTROL |
topic |
BLUEBERRY BOTRYTIS CINEREA ALTERNARIA ALTERNATA BIOLOGICAL CONTROL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Blueberry production in Argentina faces economic losses caused mainly by fungal pathogens such as Botrytis cinerea and Alternaria alternata. Synthetic fungicides are expected to be discontinued in favor of a more sustainable agriculture, making indigenous yeast, which protects the plants, a promising alternative. This study explored the cultivable portion of blueberry fruits and flowers epiphytic yeast community to identify yeasts capable of protecting fruits against the major phytopathogens affecting this crop. Native yeasts were isolated and identified based on the PCR-amplified and sequenced D1/D2 domains of the 26S rRNA gene. Members of the genera: Rhodotorula, Hanseniaspora, Naganishia, Moesziomyces, Rhizosphaera, Metschnikowia, Clavispora, Pallidophorina, Hannaella, Aureobasidium, Sporobolomyces, Filobasidium, and Cystobasidium were identified. Subsequently, antagonism against B. cinerea and A. alternata was assessed in vitro and in vivo. Finally, potential modes of action such as killer phenotype, siderophore production and formation of volatile organic compounds (VOCs) were also evaluated. Among 38 yeast strains identified, the strain Aureobasidium leucospermi LAF2 stood out in vitro and in vivo with respect to protect blueberry fruits against B. cinerea by 58.33 % and A. alternata by 67.78 % of biocontrol efficiency at 25 ◦C. It also proved the ability to produce hydroxamate-type siderophores and VOCs capable of inhibiting B. cinerea growth by 48.19 %. These results suggest that fruits and flowers epiphytic microbial communities are a reservoir of potential biological control agents. A. leucospermi LAF2 is a strong candidate for a sustainable alternative to synthetic fungicides in blueberry production. Fil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Guiñazú, Santiago Bernabé. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Alvarez, Fátima Micaela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Poehlein, Anja. Universität Göttingen; Alemania Fil: Daniel, Rolf. Universität Göttingen; Alemania Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina |
description |
Blueberry production in Argentina faces economic losses caused mainly by fungal pathogens such as Botrytis cinerea and Alternaria alternata. Synthetic fungicides are expected to be discontinued in favor of a more sustainable agriculture, making indigenous yeast, which protects the plants, a promising alternative. This study explored the cultivable portion of blueberry fruits and flowers epiphytic yeast community to identify yeasts capable of protecting fruits against the major phytopathogens affecting this crop. Native yeasts were isolated and identified based on the PCR-amplified and sequenced D1/D2 domains of the 26S rRNA gene. Members of the genera: Rhodotorula, Hanseniaspora, Naganishia, Moesziomyces, Rhizosphaera, Metschnikowia, Clavispora, Pallidophorina, Hannaella, Aureobasidium, Sporobolomyces, Filobasidium, and Cystobasidium were identified. Subsequently, antagonism against B. cinerea and A. alternata was assessed in vitro and in vivo. Finally, potential modes of action such as killer phenotype, siderophore production and formation of volatile organic compounds (VOCs) were also evaluated. Among 38 yeast strains identified, the strain Aureobasidium leucospermi LAF2 stood out in vitro and in vivo with respect to protect blueberry fruits against B. cinerea by 58.33 % and A. alternata by 67.78 % of biocontrol efficiency at 25 ◦C. It also proved the ability to produce hydroxamate-type siderophores and VOCs capable of inhibiting B. cinerea growth by 48.19 %. These results suggest that fruits and flowers epiphytic microbial communities are a reservoir of potential biological control agents. A. leucospermi LAF2 is a strong candidate for a sustainable alternative to synthetic fungicides in blueberry production. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/272002 Chacón, Florencia Isabel; Sineli, Pedro Eugenio; Guiñazú, Santiago Bernabé; Alvarez, Fátima Micaela; Poehlein, Anja; et al.; Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata; Academic Press Inc Elsevier Science; Biological Control; 209; 10-2025; 1-11 1049-9644 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/272002 |
identifier_str_mv |
Chacón, Florencia Isabel; Sineli, Pedro Eugenio; Guiñazú, Santiago Bernabé; Alvarez, Fátima Micaela; Poehlein, Anja; et al.; Unveiling the potential of native Blueberry-derived yeasts as biocontrol agents against Botrytis cinerea and Alternaria alternata; Academic Press Inc Elsevier Science; Biological Control; 209; 10-2025; 1-11 1049-9644 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S104996442500180X info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2025.105870 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613329237049344 |
score |
13.070432 |