Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium

Autores
Lajmanovich, Rafael Carlos; Cabagna Zenklusen, Mariana; Attademo, Andres Maximiliano; Junges, Celina Maria; Peltzer, Paola; Bassó, Agustín; Lorenzatti, Eduardo Antonio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The assessment of micronucleated erythrocytes (ME) in blood represents a widely used method for thedetection of chromosomal damage by chemical agents, such as herbicides that may occur as water con-taminants. We investigated the changes in some circulating blood-cell parameters of tadpoles of thecommon toad (Rhinella arenarum) that were exposed during 48 or 96 h to three sub-lethal concentra-tions (3.75, 7.5, and 15 mg/L) of a commercial formulation of a glufosinate-ammonium (GLA)-basedherbicide (Liberty®, LY®) as well as to the corresponding active ingredient GLA. The frequency of MEand other erythrocyte nuclear abnormalities (ENA, i.e., lobed nuclei, binucleates or segmented nuclei,kidney-shaped nuclei, notched nuclei, and picnotic nuclei) were evaluated and compared with positive(cyclophosphamide, CP, 40 mg/L) and negative (de-chlorinated tap water) controls. The results indicatethat the exposure of R. arenarum tadpoles to LY®induces a concentration-dependent increase in ME fre-quency. The ENA frequency at 48 h was also significantly higher than that in the negative control groupfor all the chemicals assayed (CP, LY®and GLA) whereas at 96 h, increases in ENA over the negative controlgroup were found only for CP and GLA (7.5 mg/L). Our study demonstrates that the commercial formula-tion of a GLA-based herbicide induces micronucleus formation in R. arenarum tadpoles, in contrast to theactive ingredient. According to these results, the inert ingredients of the commercial formulation playedan important role in the production of genotoxic damage in erythrocytes of amphibian tadpoles.
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabagna Zenklusen, Mariana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bassó, Agustín. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Lorenzatti, Eduardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Materia
Glufosinate
Tadpoles
Mutagenesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9581
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/9581
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammoniumLajmanovich, Rafael CarlosCabagna Zenklusen, MarianaAttademo, Andres MaximilianoJunges, Celina MariaPeltzer, PaolaBassó, AgustínLorenzatti, Eduardo AntonioGlufosinateTadpolesMutagenesishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The assessment of micronucleated erythrocytes (ME) in blood represents a widely used method for thedetection of chromosomal damage by chemical agents, such as herbicides that may occur as water con-taminants. We investigated the changes in some circulating blood-cell parameters of tadpoles of thecommon toad (Rhinella arenarum) that were exposed during 48 or 96 h to three sub-lethal concentra-tions (3.75, 7.5, and 15 mg/L) of a commercial formulation of a glufosinate-ammonium (GLA)-basedherbicide (Liberty®, LY®) as well as to the corresponding active ingredient GLA. The frequency of MEand other erythrocyte nuclear abnormalities (ENA, i.e., lobed nuclei, binucleates or segmented nuclei,kidney-shaped nuclei, notched nuclei, and picnotic nuclei) were evaluated and compared with positive(cyclophosphamide, CP, 40 mg/L) and negative (de-chlorinated tap water) controls. The results indicatethat the exposure of R. arenarum tadpoles to LY®induces a concentration-dependent increase in ME fre-quency. The ENA frequency at 48 h was also significantly higher than that in the negative control groupfor all the chemicals assayed (CP, LY®and GLA) whereas at 96 h, increases in ENA over the negative controlgroup were found only for CP and GLA (7.5 mg/L). Our study demonstrates that the commercial formula-tion of a GLA-based herbicide induces micronucleus formation in R. arenarum tadpoles, in contrast to theactive ingredient. According to these results, the inert ingredients of the commercial formulation playedan important role in the production of genotoxic damage in erythrocytes of amphibian tadpoles.Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabagna Zenklusen, Mariana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bassó, Agustín. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Lorenzatti, Eduardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); ArgentinaElsevier Science2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9581Lajmanovich, Rafael Carlos; Cabagna Zenklusen, Mariana; Attademo, Andres Maximiliano; Junges, Celina Maria; Peltzer, Paola; et al.; Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 769; 1-2014; 1-221383-5718enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2014.04.009info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S138357181400103Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:49Zoai:ri.conicet.gov.ar:11336/9581instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:49.424CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium
title Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium
spellingShingle Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium
Lajmanovich, Rafael Carlos
Glufosinate
Tadpoles
Mutagenesis
title_short Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium
title_full Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium
title_fullStr Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium
title_full_unstemmed Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium
title_sort Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium
dc.creator.none.fl_str_mv Lajmanovich, Rafael Carlos
Cabagna Zenklusen, Mariana
Attademo, Andres Maximiliano
Junges, Celina Maria
Peltzer, Paola
Bassó, Agustín
Lorenzatti, Eduardo Antonio
author Lajmanovich, Rafael Carlos
author_facet Lajmanovich, Rafael Carlos
Cabagna Zenklusen, Mariana
Attademo, Andres Maximiliano
Junges, Celina Maria
Peltzer, Paola
Bassó, Agustín
Lorenzatti, Eduardo Antonio
author_role author
author2 Cabagna Zenklusen, Mariana
Attademo, Andres Maximiliano
Junges, Celina Maria
Peltzer, Paola
Bassó, Agustín
Lorenzatti, Eduardo Antonio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Glufosinate
Tadpoles
Mutagenesis
topic Glufosinate
Tadpoles
Mutagenesis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The assessment of micronucleated erythrocytes (ME) in blood represents a widely used method for thedetection of chromosomal damage by chemical agents, such as herbicides that may occur as water con-taminants. We investigated the changes in some circulating blood-cell parameters of tadpoles of thecommon toad (Rhinella arenarum) that were exposed during 48 or 96 h to three sub-lethal concentra-tions (3.75, 7.5, and 15 mg/L) of a commercial formulation of a glufosinate-ammonium (GLA)-basedherbicide (Liberty®, LY®) as well as to the corresponding active ingredient GLA. The frequency of MEand other erythrocyte nuclear abnormalities (ENA, i.e., lobed nuclei, binucleates or segmented nuclei,kidney-shaped nuclei, notched nuclei, and picnotic nuclei) were evaluated and compared with positive(cyclophosphamide, CP, 40 mg/L) and negative (de-chlorinated tap water) controls. The results indicatethat the exposure of R. arenarum tadpoles to LY®induces a concentration-dependent increase in ME fre-quency. The ENA frequency at 48 h was also significantly higher than that in the negative control groupfor all the chemicals assayed (CP, LY®and GLA) whereas at 96 h, increases in ENA over the negative controlgroup were found only for CP and GLA (7.5 mg/L). Our study demonstrates that the commercial formula-tion of a GLA-based herbicide induces micronucleus formation in R. arenarum tadpoles, in contrast to theactive ingredient. According to these results, the inert ingredients of the commercial formulation playedan important role in the production of genotoxic damage in erythrocytes of amphibian tadpoles.
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabagna Zenklusen, Mariana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bassó, Agustín. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Lorenzatti, Eduardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
description The assessment of micronucleated erythrocytes (ME) in blood represents a widely used method for thedetection of chromosomal damage by chemical agents, such as herbicides that may occur as water con-taminants. We investigated the changes in some circulating blood-cell parameters of tadpoles of thecommon toad (Rhinella arenarum) that were exposed during 48 or 96 h to three sub-lethal concentra-tions (3.75, 7.5, and 15 mg/L) of a commercial formulation of a glufosinate-ammonium (GLA)-basedherbicide (Liberty®, LY®) as well as to the corresponding active ingredient GLA. The frequency of MEand other erythrocyte nuclear abnormalities (ENA, i.e., lobed nuclei, binucleates or segmented nuclei,kidney-shaped nuclei, notched nuclei, and picnotic nuclei) were evaluated and compared with positive(cyclophosphamide, CP, 40 mg/L) and negative (de-chlorinated tap water) controls. The results indicatethat the exposure of R. arenarum tadpoles to LY®induces a concentration-dependent increase in ME fre-quency. The ENA frequency at 48 h was also significantly higher than that in the negative control groupfor all the chemicals assayed (CP, LY®and GLA) whereas at 96 h, increases in ENA over the negative controlgroup were found only for CP and GLA (7.5 mg/L). Our study demonstrates that the commercial formula-tion of a GLA-based herbicide induces micronucleus formation in R. arenarum tadpoles, in contrast to theactive ingredient. According to these results, the inert ingredients of the commercial formulation playedan important role in the production of genotoxic damage in erythrocytes of amphibian tadpoles.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9581
Lajmanovich, Rafael Carlos; Cabagna Zenklusen, Mariana; Attademo, Andres Maximiliano; Junges, Celina Maria; Peltzer, Paola; et al.; Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 769; 1-2014; 1-22
1383-5718
url http://hdl.handle.net/11336/9581
identifier_str_mv Lajmanovich, Rafael Carlos; Cabagna Zenklusen, Mariana; Attademo, Andres Maximiliano; Junges, Celina Maria; Peltzer, Paola; et al.; Induction of micronuclei and nuclear abnormalities in tadpoles of the common toad (Rhinella arenarum) treated with the herbicides Liberty® and glufosinate-ammonium; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 769; 1-2014; 1-22
1383-5718
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2014.04.009
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S138357181400103X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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