Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication

Autores
Covacevich, Fernanda; Hernandez Guijarro, Keren; Crespo, Esteban; Lumini, Erica; Rivero Mega, María Soledad; Lugo, Mónica Alejandra
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Low arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) analysis after trap plant multiplication strategies to improve the knowledge of the current richness of glomalean AM fungi (Glomerales; Glomeromycota) from the Argentine Puna. Indigenous propagules from two pristine sites at 3870 and 3370 m of elevation were multiplied using different host plants; propagation periods (2–6 months), and subculture cycles (1; 2; or 3) from 5 to 13 months. The propagule multiplication experiment allowed the detection of different glomoid taxa of Funneliformis spp. and Rhizoglomus spp., which were considered cryptic species since they had never been found in Puna soils before. On the other hand; almost all the generalist species previously described were recovered from cultures; except for Glomus ambisporum. Both plant host selection and culture times are critical for Glomerales multiplication. The SSCP analysis complemented the morphological approach and showed a high variability of Glomus at each site; revealing the presence of Funneliformis mosseae. This study demonstrates that AMF trap culture (TC) is a useful strategy for improving the analysis of AM fungal diversity/richness in the Argentinean highlands.
EEA Balcarce
Fil: Covacevich, Fernanada. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación Biológicas Aplicadas. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina.
Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Crespo, Esteban. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.
Fil: Lumini, Erica. Institute for Sustainable Plant Protection of National Research Council; Italia.
Fil: Rivero Mega, María Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.
Fil: Lugo, Mónica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.
Fil: Lugo, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis; Argentina. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina.
Fuente
Plants 10 (9) : 1803 (September 2021)
Materia
Suelo
Hongos
Biodiversidad
Micorrizas Arbusculares
Propágulos
Zona de Montaña
Argentina
Soil
Fungi
Biodiversity
Arbuscular Mycorrhiza
Sets
Highlands
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10249
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10249
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplicationCovacevich, FernandaHernandez Guijarro, KerenCrespo, EstebanLumini, EricaRivero Mega, María SoledadLugo, Mónica AlejandraSueloHongosBiodiversidadMicorrizas ArbuscularesPropágulosZona de MontañaArgentinaSoilFungiBiodiversityArbuscular MycorrhizaSetsHighlandsLow arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) analysis after trap plant multiplication strategies to improve the knowledge of the current richness of glomalean AM fungi (Glomerales; Glomeromycota) from the Argentine Puna. Indigenous propagules from two pristine sites at 3870 and 3370 m of elevation were multiplied using different host plants; propagation periods (2–6 months), and subculture cycles (1; 2; or 3) from 5 to 13 months. The propagule multiplication experiment allowed the detection of different glomoid taxa of Funneliformis spp. and Rhizoglomus spp., which were considered cryptic species since they had never been found in Puna soils before. On the other hand; almost all the generalist species previously described were recovered from cultures; except for Glomus ambisporum. Both plant host selection and culture times are critical for Glomerales multiplication. The SSCP analysis complemented the morphological approach and showed a high variability of Glomus at each site; revealing the presence of Funneliformis mosseae. This study demonstrates that AMF trap culture (TC) is a useful strategy for improving the analysis of AM fungal diversity/richness in the Argentinean highlands.EEA BalcarceFil: Covacevich, Fernanada. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación Biológicas Aplicadas. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina.Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Crespo, Esteban. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.Fil: Lumini, Erica. Institute for Sustainable Plant Protection of National Research Council; Italia.Fil: Rivero Mega, María Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.Fil: Lugo, Mónica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.Fil: Lugo, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis; Argentina. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina.Multidisciplinary Digital Publishing Institute (MDPI)2021-09-14T11:45:50Z2021-09-14T11:45:50Z2021-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10249https://www.mdpi.com/2223-7747/10/9/1803/html2223-7747https://doi.org/10.3390/plants10091803Plants 10 (9) : 1803 (September 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E2-I037-002/2019-PD-E2-I037-002/AR./Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso sueloinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:20Zoai:localhost:20.500.12123/10249instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:20.878INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
title Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
spellingShingle Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
Covacevich, Fernanda
Suelo
Hongos
Biodiversidad
Micorrizas Arbusculares
Propágulos
Zona de Montaña
Argentina
Soil
Fungi
Biodiversity
Arbuscular Mycorrhiza
Sets
Highlands
title_short Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
title_full Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
title_fullStr Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
title_full_unstemmed Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
title_sort Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
dc.creator.none.fl_str_mv Covacevich, Fernanda
Hernandez Guijarro, Keren
Crespo, Esteban
Lumini, Erica
Rivero Mega, María Soledad
Lugo, Mónica Alejandra
author Covacevich, Fernanda
author_facet Covacevich, Fernanda
Hernandez Guijarro, Keren
Crespo, Esteban
Lumini, Erica
Rivero Mega, María Soledad
Lugo, Mónica Alejandra
author_role author
author2 Hernandez Guijarro, Keren
Crespo, Esteban
Lumini, Erica
Rivero Mega, María Soledad
Lugo, Mónica Alejandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Suelo
Hongos
Biodiversidad
Micorrizas Arbusculares
Propágulos
Zona de Montaña
Argentina
Soil
Fungi
Biodiversity
Arbuscular Mycorrhiza
Sets
Highlands
topic Suelo
Hongos
Biodiversidad
Micorrizas Arbusculares
Propágulos
Zona de Montaña
Argentina
Soil
Fungi
Biodiversity
Arbuscular Mycorrhiza
Sets
Highlands
dc.description.none.fl_txt_mv Low arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) analysis after trap plant multiplication strategies to improve the knowledge of the current richness of glomalean AM fungi (Glomerales; Glomeromycota) from the Argentine Puna. Indigenous propagules from two pristine sites at 3870 and 3370 m of elevation were multiplied using different host plants; propagation periods (2–6 months), and subculture cycles (1; 2; or 3) from 5 to 13 months. The propagule multiplication experiment allowed the detection of different glomoid taxa of Funneliformis spp. and Rhizoglomus spp., which were considered cryptic species since they had never been found in Puna soils before. On the other hand; almost all the generalist species previously described were recovered from cultures; except for Glomus ambisporum. Both plant host selection and culture times are critical for Glomerales multiplication. The SSCP analysis complemented the morphological approach and showed a high variability of Glomus at each site; revealing the presence of Funneliformis mosseae. This study demonstrates that AMF trap culture (TC) is a useful strategy for improving the analysis of AM fungal diversity/richness in the Argentinean highlands.
EEA Balcarce
Fil: Covacevich, Fernanada. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación Biológicas Aplicadas. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina.
Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Crespo, Esteban. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.
Fil: Lumini, Erica. Institute for Sustainable Plant Protection of National Research Council; Italia.
Fil: Rivero Mega, María Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.
Fil: Lugo, Mónica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.
Fil: Lugo, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis; Argentina. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina.
description Low arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) analysis after trap plant multiplication strategies to improve the knowledge of the current richness of glomalean AM fungi (Glomerales; Glomeromycota) from the Argentine Puna. Indigenous propagules from two pristine sites at 3870 and 3370 m of elevation were multiplied using different host plants; propagation periods (2–6 months), and subculture cycles (1; 2; or 3) from 5 to 13 months. The propagule multiplication experiment allowed the detection of different glomoid taxa of Funneliformis spp. and Rhizoglomus spp., which were considered cryptic species since they had never been found in Puna soils before. On the other hand; almost all the generalist species previously described were recovered from cultures; except for Glomus ambisporum. Both plant host selection and culture times are critical for Glomerales multiplication. The SSCP analysis complemented the morphological approach and showed a high variability of Glomus at each site; revealing the presence of Funneliformis mosseae. This study demonstrates that AMF trap culture (TC) is a useful strategy for improving the analysis of AM fungal diversity/richness in the Argentinean highlands.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-14T11:45:50Z
2021-09-14T11:45:50Z
2021-08-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10249
https://www.mdpi.com/2223-7747/10/9/1803/html
2223-7747
https://doi.org/10.3390/plants10091803
url http://hdl.handle.net/20.500.12123/10249
https://www.mdpi.com/2223-7747/10/9/1803/html
https://doi.org/10.3390/plants10091803
identifier_str_mv 2223-7747
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002/2019-PD-E2-I037-002/AR./Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv Plants 10 (9) : 1803 (September 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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