Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor

Autores
Barrios Baron, Maria Pilar; Delfosse, Veronica Cecilia; Agrofoglio, Yamila Carla; Nahirñak, Vanesa; Almasia, Natalia Ines; Vazquez Rovere, Cecilia; Distefano, Ana Julia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL‐Ar impedes accumulation of dsRNA‐derived siRNAs. We also showed that P0PL‐Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F‐box‐like domain. A mutant in the GW/WG motif within the P0PL‐Ar F‐box‐like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL‐Ar F‐box‐like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL‐Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer‐like enzyme (DCL)‐mediated primary siRNA production and downstream at the RNA‐induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.
Instituto de Biotecnología
Fil: Barrios Baron, Maria Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Barrios Baron, Maria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Delfosse, Veronica Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delfosse, Veronica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Agrofoglio, Yamila Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Nahirñak, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Almasia, Natalia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Vazquez Rovere, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Distefano, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Distefano, Ana Julia. Universidad Nacional de Luján (UNLU); Argentina
Fuente
Plant Pathology 70 (2) : 259-274 (Febrero 2021)
Materia
Potato Leaf Roll Virus
Immunosuppressants
Luteovirus del Enrollado de Patata
Solanum tuberosum
Inmunodepresores
Argonaute
Argonauta
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9088
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9088
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spelling Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressorBarrios Baron, Maria PilarDelfosse, Veronica CeciliaAgrofoglio, Yamila CarlaNahirñak, VanesaAlmasia, Natalia InesVazquez Rovere, CeciliaDistefano, Ana JuliaPotato Leaf Roll VirusImmunosuppressantsLuteovirus del Enrollado de PatataSolanum tuberosumInmunodepresoresArgonauteArgonautaThe potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL‐Ar impedes accumulation of dsRNA‐derived siRNAs. We also showed that P0PL‐Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F‐box‐like domain. A mutant in the GW/WG motif within the P0PL‐Ar F‐box‐like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL‐Ar F‐box‐like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL‐Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer‐like enzyme (DCL)‐mediated primary siRNA production and downstream at the RNA‐induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.Instituto de BiotecnologíaFil: Barrios Baron, Maria Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Barrios Baron, Maria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Delfosse, Veronica Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Delfosse, Veronica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Agrofoglio, Yamila Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Nahirñak, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Almasia, Natalia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Vazquez Rovere, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Distefano, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Distefano, Ana Julia. Universidad Nacional de Luján (UNLU); ArgentinaWiley2021-04-14T15:57:32Z2021-04-14T15:57:32Z2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9088https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.132901365-3059https://doi.org/10.1111/ppa.13290Plant Pathology 70 (2) : 259-274 (Febrero 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.info:eu-repo/semantics/restrictedAccess2025-10-23T11:17:33Zoai:localhost:20.500.12123/9088instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:33.678INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
title Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
spellingShingle Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
Barrios Baron, Maria Pilar
Potato Leaf Roll Virus
Immunosuppressants
Luteovirus del Enrollado de Patata
Solanum tuberosum
Inmunodepresores
Argonaute
Argonauta
title_short Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
title_full Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
title_fullStr Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
title_full_unstemmed Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
title_sort Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
dc.creator.none.fl_str_mv Barrios Baron, Maria Pilar
Delfosse, Veronica Cecilia
Agrofoglio, Yamila Carla
Nahirñak, Vanesa
Almasia, Natalia Ines
Vazquez Rovere, Cecilia
Distefano, Ana Julia
author Barrios Baron, Maria Pilar
author_facet Barrios Baron, Maria Pilar
Delfosse, Veronica Cecilia
Agrofoglio, Yamila Carla
Nahirñak, Vanesa
Almasia, Natalia Ines
Vazquez Rovere, Cecilia
Distefano, Ana Julia
author_role author
author2 Delfosse, Veronica Cecilia
Agrofoglio, Yamila Carla
Nahirñak, Vanesa
Almasia, Natalia Ines
Vazquez Rovere, Cecilia
Distefano, Ana Julia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Potato Leaf Roll Virus
Immunosuppressants
Luteovirus del Enrollado de Patata
Solanum tuberosum
Inmunodepresores
Argonaute
Argonauta
topic Potato Leaf Roll Virus
Immunosuppressants
Luteovirus del Enrollado de Patata
Solanum tuberosum
Inmunodepresores
Argonaute
Argonauta
dc.description.none.fl_txt_mv The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL‐Ar impedes accumulation of dsRNA‐derived siRNAs. We also showed that P0PL‐Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F‐box‐like domain. A mutant in the GW/WG motif within the P0PL‐Ar F‐box‐like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL‐Ar F‐box‐like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL‐Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer‐like enzyme (DCL)‐mediated primary siRNA production and downstream at the RNA‐induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.
Instituto de Biotecnología
Fil: Barrios Baron, Maria Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Barrios Baron, Maria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Delfosse, Veronica Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delfosse, Veronica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Agrofoglio, Yamila Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Nahirñak, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Almasia, Natalia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Vazquez Rovere, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Distefano, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Distefano, Ana Julia. Universidad Nacional de Luján (UNLU); Argentina
description The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL‐Ar impedes accumulation of dsRNA‐derived siRNAs. We also showed that P0PL‐Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F‐box‐like domain. A mutant in the GW/WG motif within the P0PL‐Ar F‐box‐like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL‐Ar F‐box‐like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL‐Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer‐like enzyme (DCL)‐mediated primary siRNA production and downstream at the RNA‐induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-14T15:57:32Z
2021-04-14T15:57:32Z
2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9088
https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13290
1365-3059
https://doi.org/10.1111/ppa.13290
url http://hdl.handle.net/20.500.12123/9088
https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13290
https://doi.org/10.1111/ppa.13290
identifier_str_mv 1365-3059
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant Pathology 70 (2) : 259-274 (Febrero 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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