Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars

Autores
Gatica Hernandez, Ismaél Jairo Gabriel; Palottini, Florencia; Macri, Ivana Noelia; Galmarini, Claudio Romulo; Farina, Walter Marcelo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The honey bee is the most frequently used species in pollination services for diverse crops. In onion crops (Allium cepa L.), however, bees avoid visiting certain varieties, being attracted differently to male sterile (MS) and fertile (OP) lines. These differences might be based on the phenolic profiles of the cultivars’ nectars. To understand the relationship between nectar composition and pollinator attraction to different onion lines, we tested sensory and cognitive abilities and palatability in honey bees exposed to MS and OP onion nectars and sugar solutions mimicking them. We evaluated the proboscis extension response (PER) after antennal contact (unconditioned response) to MS or OP onion nectars, finding no statistical differences, which denotes similar gustatory perception for both nectars. We also performed food uptake assays to test palatability of different artificial nectars, considering their flavonoids and potassium content. The presence of potassium decreased palatability of the artificial nectars. Finally, we evaluated the beeś cognitive abilities when the reward (unconditioned stimulus, US) offered during conditioning PER assays presents differences in composition. We found that potassium by itself impaired learning; however, such impairment was even higher when naringenin and quercetin were added in the US (MS mimic nectar). Interestingly, potassium together with luteolin (OP mimic nectar) improved learning. Our study demonstrates that the differences in the nectars’ flavonoid profiles combined with their high potassium content could explain the previously reported differences in attractiveness between onion lines, suggesting an important role of nectar-compounds other than sugars for the attractiveness of flowers to pollinators.
EEA La Consulta
Fil: Gatica Hernandez, Ismaél Jairo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palottini, Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Macri, Ivana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Ingenierıá Rural; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Farina, Walter Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of experimental biology 222 (2) : jeb.189910. (January 2019)
Materia
Apis Mellifera
Comportamiento Animal
Apetito
Néctar
Olfacción
Compuestos Fenólicos
Alimentación de los Animales
Flavonoides
Polinización
Animal Behaviour
Appetite
Olfaction
Phenolic Compounds
Animal Feeding
Flavonoids
Pollination
Olfactory Learning
Responsiveness
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4929
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4929
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network_name_str INTA Digital (INTA)
spelling Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectarsGatica Hernandez, Ismaél Jairo GabrielPalottini, FlorenciaMacri, Ivana NoeliaGalmarini, Claudio RomuloFarina, Walter MarceloApis MelliferaComportamiento AnimalApetitoNéctarOlfacciónCompuestos FenólicosAlimentación de los AnimalesFlavonoidesPolinizaciónAnimal BehaviourAppetiteOlfactionPhenolic CompoundsAnimal FeedingFlavonoidsPollinationOlfactory LearningResponsivenessThe honey bee is the most frequently used species in pollination services for diverse crops. In onion crops (Allium cepa L.), however, bees avoid visiting certain varieties, being attracted differently to male sterile (MS) and fertile (OP) lines. These differences might be based on the phenolic profiles of the cultivars’ nectars. To understand the relationship between nectar composition and pollinator attraction to different onion lines, we tested sensory and cognitive abilities and palatability in honey bees exposed to MS and OP onion nectars and sugar solutions mimicking them. We evaluated the proboscis extension response (PER) after antennal contact (unconditioned response) to MS or OP onion nectars, finding no statistical differences, which denotes similar gustatory perception for both nectars. We also performed food uptake assays to test palatability of different artificial nectars, considering their flavonoids and potassium content. The presence of potassium decreased palatability of the artificial nectars. Finally, we evaluated the beeś cognitive abilities when the reward (unconditioned stimulus, US) offered during conditioning PER assays presents differences in composition. We found that potassium by itself impaired learning; however, such impairment was even higher when naringenin and quercetin were added in the US (MS mimic nectar). Interestingly, potassium together with luteolin (OP mimic nectar) improved learning. Our study demonstrates that the differences in the nectars’ flavonoid profiles combined with their high potassium content could explain the previously reported differences in attractiveness between onion lines, suggesting an important role of nectar-compounds other than sugars for the attractiveness of flowers to pollinators.EEA La ConsultaFil: Gatica Hernandez, Ismaél Jairo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palottini, Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Macri, Ivana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Ingenierıá Rural; ArgentinaFil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Farina, Walter Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCompany of Biologists2019-04-17T12:51:26Z2019-04-17T12:51:26Z2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4929http://jeb.biologists.org/content/222/2/jeb1899100022-09491477-9145 (Online)http://dx.doi.org/10.1242/jeb.189910Journal of experimental biology 222 (2) : jeb.189910. (January 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:38Zoai:localhost:20.500.12123/4929instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:38.438INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
title Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
spellingShingle Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
Gatica Hernandez, Ismaél Jairo Gabriel
Apis Mellifera
Comportamiento Animal
Apetito
Néctar
Olfacción
Compuestos Fenólicos
Alimentación de los Animales
Flavonoides
Polinización
Animal Behaviour
Appetite
Olfaction
Phenolic Compounds
Animal Feeding
Flavonoids
Pollination
Olfactory Learning
Responsiveness
title_short Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
title_full Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
title_fullStr Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
title_full_unstemmed Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
title_sort Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
dc.creator.none.fl_str_mv Gatica Hernandez, Ismaél Jairo Gabriel
Palottini, Florencia
Macri, Ivana Noelia
Galmarini, Claudio Romulo
Farina, Walter Marcelo
author Gatica Hernandez, Ismaél Jairo Gabriel
author_facet Gatica Hernandez, Ismaél Jairo Gabriel
Palottini, Florencia
Macri, Ivana Noelia
Galmarini, Claudio Romulo
Farina, Walter Marcelo
author_role author
author2 Palottini, Florencia
Macri, Ivana Noelia
Galmarini, Claudio Romulo
Farina, Walter Marcelo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Apis Mellifera
Comportamiento Animal
Apetito
Néctar
Olfacción
Compuestos Fenólicos
Alimentación de los Animales
Flavonoides
Polinización
Animal Behaviour
Appetite
Olfaction
Phenolic Compounds
Animal Feeding
Flavonoids
Pollination
Olfactory Learning
Responsiveness
topic Apis Mellifera
Comportamiento Animal
Apetito
Néctar
Olfacción
Compuestos Fenólicos
Alimentación de los Animales
Flavonoides
Polinización
Animal Behaviour
Appetite
Olfaction
Phenolic Compounds
Animal Feeding
Flavonoids
Pollination
Olfactory Learning
Responsiveness
dc.description.none.fl_txt_mv The honey bee is the most frequently used species in pollination services for diverse crops. In onion crops (Allium cepa L.), however, bees avoid visiting certain varieties, being attracted differently to male sterile (MS) and fertile (OP) lines. These differences might be based on the phenolic profiles of the cultivars’ nectars. To understand the relationship between nectar composition and pollinator attraction to different onion lines, we tested sensory and cognitive abilities and palatability in honey bees exposed to MS and OP onion nectars and sugar solutions mimicking them. We evaluated the proboscis extension response (PER) after antennal contact (unconditioned response) to MS or OP onion nectars, finding no statistical differences, which denotes similar gustatory perception for both nectars. We also performed food uptake assays to test palatability of different artificial nectars, considering their flavonoids and potassium content. The presence of potassium decreased palatability of the artificial nectars. Finally, we evaluated the beeś cognitive abilities when the reward (unconditioned stimulus, US) offered during conditioning PER assays presents differences in composition. We found that potassium by itself impaired learning; however, such impairment was even higher when naringenin and quercetin were added in the US (MS mimic nectar). Interestingly, potassium together with luteolin (OP mimic nectar) improved learning. Our study demonstrates that the differences in the nectars’ flavonoid profiles combined with their high potassium content could explain the previously reported differences in attractiveness between onion lines, suggesting an important role of nectar-compounds other than sugars for the attractiveness of flowers to pollinators.
EEA La Consulta
Fil: Gatica Hernandez, Ismaél Jairo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palottini, Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Macri, Ivana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Ingenierıá Rural; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Farina, Walter Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Fisiología de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The honey bee is the most frequently used species in pollination services for diverse crops. In onion crops (Allium cepa L.), however, bees avoid visiting certain varieties, being attracted differently to male sterile (MS) and fertile (OP) lines. These differences might be based on the phenolic profiles of the cultivars’ nectars. To understand the relationship between nectar composition and pollinator attraction to different onion lines, we tested sensory and cognitive abilities and palatability in honey bees exposed to MS and OP onion nectars and sugar solutions mimicking them. We evaluated the proboscis extension response (PER) after antennal contact (unconditioned response) to MS or OP onion nectars, finding no statistical differences, which denotes similar gustatory perception for both nectars. We also performed food uptake assays to test palatability of different artificial nectars, considering their flavonoids and potassium content. The presence of potassium decreased palatability of the artificial nectars. Finally, we evaluated the beeś cognitive abilities when the reward (unconditioned stimulus, US) offered during conditioning PER assays presents differences in composition. We found that potassium by itself impaired learning; however, such impairment was even higher when naringenin and quercetin were added in the US (MS mimic nectar). Interestingly, potassium together with luteolin (OP mimic nectar) improved learning. Our study demonstrates that the differences in the nectars’ flavonoid profiles combined with their high potassium content could explain the previously reported differences in attractiveness between onion lines, suggesting an important role of nectar-compounds other than sugars for the attractiveness of flowers to pollinators.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-17T12:51:26Z
2019-04-17T12:51:26Z
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4929
http://jeb.biologists.org/content/222/2/jeb189910
0022-0949
1477-9145 (Online)
http://dx.doi.org/10.1242/jeb.189910
url http://hdl.handle.net/20.500.12123/4929
http://jeb.biologists.org/content/222/2/jeb189910
http://dx.doi.org/10.1242/jeb.189910
identifier_str_mv 0022-0949
1477-9145 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv Journal of experimental biology 222 (2) : jeb.189910. (January 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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