Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos

Autores
Cagnoli, Claudia Inés; Chiapparrone, María Laura; Cacciato, Claudio Santiago; Rodríguez, Marcelo Gastón; Catena, María del Carmen; Aller Atucha, Juan Florencio
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Campylobacter fetus is an important veterinary pathogen that causes campylobacteriosis. This disease causes decreased productivity of cattle by inducing reproductive losses. Although several virulence factors have been recognized in C. fetus, including a cytolethal distending toxin (CDT), the exact mechanism responsible for embryonic death remains unknown. The aim of this work was to evaluate the effect of C. fetus subsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv), and their toxin activity on the in vitro fertilization of bovine ova and early embryonic development. Two different experiments were performed. In Experiment 1, a total of 1524 cumulus–oocyte complexes (COCs) were inseminated, distributed into three groups: two of them infected with the microorganism (Cff, Cfv) and a control group. Percentages COCs cleaved were similar among groups (p = 0.1243); however, the embryonic development rate (blastocyst at day 7) in the control group was greater (p < 0.001) than those obtained in Cff and Cfv groups. In experiment 2, a total of 746 COCs were inseminated, divided into three groups: two of them treated with the bacterial-free culture filtrates to test toxin activity (Cff-CDT, Cfv-CDT) and a control group. Both cleavage and embryonic development rates were greater (p < 0.001) in the control group than those obtained in Cff-CDT and Cfv-CDT groups. This study provides evidence that both subspecies of C. fetus do not interfere with fertilization but do affect in vitro embryonic development. It is the first report on the biological effect of the CDT on bovine embryonic development.
EEA Balcarce
Fil: Cagnoli, Claudia Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.
Fil: Chiapparrone, María Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.
Fil: Cacciato, Claudio Santiago. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.
Fil: Rodríguez, Marcelo Gastón. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Catena, María del Carmen. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fuente
Veterinary Microbiology 288 : 109925 (January 2024)
Materia
Campylobacter
Bovinae
Pathogenesis
Animal Embryos
Animal Reproduction
Patogénesis
Embriones Animales
In Vitro
Reproducción Animal
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16814
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16814
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryosCagnoli, Claudia InésChiapparrone, María LauraCacciato, Claudio SantiagoRodríguez, Marcelo GastónCatena, María del CarmenAller Atucha, Juan FlorencioCampylobacterBovinaePathogenesisAnimal EmbryosAnimal ReproductionPatogénesisEmbriones AnimalesIn VitroReproducción AnimalCampylobacter fetus is an important veterinary pathogen that causes campylobacteriosis. This disease causes decreased productivity of cattle by inducing reproductive losses. Although several virulence factors have been recognized in C. fetus, including a cytolethal distending toxin (CDT), the exact mechanism responsible for embryonic death remains unknown. The aim of this work was to evaluate the effect of C. fetus subsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv), and their toxin activity on the in vitro fertilization of bovine ova and early embryonic development. Two different experiments were performed. In Experiment 1, a total of 1524 cumulus–oocyte complexes (COCs) were inseminated, distributed into three groups: two of them infected with the microorganism (Cff, Cfv) and a control group. Percentages COCs cleaved were similar among groups (p = 0.1243); however, the embryonic development rate (blastocyst at day 7) in the control group was greater (p < 0.001) than those obtained in Cff and Cfv groups. In experiment 2, a total of 746 COCs were inseminated, divided into three groups: two of them treated with the bacterial-free culture filtrates to test toxin activity (Cff-CDT, Cfv-CDT) and a control group. Both cleavage and embryonic development rates were greater (p < 0.001) in the control group than those obtained in Cff-CDT and Cfv-CDT groups. This study provides evidence that both subspecies of C. fetus do not interfere with fertilization but do affect in vitro embryonic development. It is the first report on the biological effect of the CDT on bovine embryonic development.EEA BalcarceFil: Cagnoli, Claudia Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.Fil: Chiapparrone, María Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.Fil: Cacciato, Claudio Santiago. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.Fil: Rodríguez, Marcelo Gastón. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Catena, María del Carmen. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Elsevier2024-02-28T10:43:08Z2024-02-28T10:43:08Z2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16814https://www.sciencedirect.com/science/article/pii/S03781135230027911873-2542 (online)0378-1135 (print)https://doi.org/10.1016/j.vetmic.2023.109925Veterinary Microbiology 288 : 109925 (January 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:14Zoai:localhost:20.500.12123/16814instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:14.573INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
title Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
spellingShingle Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
Cagnoli, Claudia Inés
Campylobacter
Bovinae
Pathogenesis
Animal Embryos
Animal Reproduction
Patogénesis
Embriones Animales
In Vitro
Reproducción Animal
title_short Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
title_full Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
title_fullStr Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
title_full_unstemmed Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
title_sort Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
dc.creator.none.fl_str_mv Cagnoli, Claudia Inés
Chiapparrone, María Laura
Cacciato, Claudio Santiago
Rodríguez, Marcelo Gastón
Catena, María del Carmen
Aller Atucha, Juan Florencio
author Cagnoli, Claudia Inés
author_facet Cagnoli, Claudia Inés
Chiapparrone, María Laura
Cacciato, Claudio Santiago
Rodríguez, Marcelo Gastón
Catena, María del Carmen
Aller Atucha, Juan Florencio
author_role author
author2 Chiapparrone, María Laura
Cacciato, Claudio Santiago
Rodríguez, Marcelo Gastón
Catena, María del Carmen
Aller Atucha, Juan Florencio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Campylobacter
Bovinae
Pathogenesis
Animal Embryos
Animal Reproduction
Patogénesis
Embriones Animales
In Vitro
Reproducción Animal
topic Campylobacter
Bovinae
Pathogenesis
Animal Embryos
Animal Reproduction
Patogénesis
Embriones Animales
In Vitro
Reproducción Animal
dc.description.none.fl_txt_mv Campylobacter fetus is an important veterinary pathogen that causes campylobacteriosis. This disease causes decreased productivity of cattle by inducing reproductive losses. Although several virulence factors have been recognized in C. fetus, including a cytolethal distending toxin (CDT), the exact mechanism responsible for embryonic death remains unknown. The aim of this work was to evaluate the effect of C. fetus subsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv), and their toxin activity on the in vitro fertilization of bovine ova and early embryonic development. Two different experiments were performed. In Experiment 1, a total of 1524 cumulus–oocyte complexes (COCs) were inseminated, distributed into three groups: two of them infected with the microorganism (Cff, Cfv) and a control group. Percentages COCs cleaved were similar among groups (p = 0.1243); however, the embryonic development rate (blastocyst at day 7) in the control group was greater (p < 0.001) than those obtained in Cff and Cfv groups. In experiment 2, a total of 746 COCs were inseminated, divided into three groups: two of them treated with the bacterial-free culture filtrates to test toxin activity (Cff-CDT, Cfv-CDT) and a control group. Both cleavage and embryonic development rates were greater (p < 0.001) in the control group than those obtained in Cff-CDT and Cfv-CDT groups. This study provides evidence that both subspecies of C. fetus do not interfere with fertilization but do affect in vitro embryonic development. It is the first report on the biological effect of the CDT on bovine embryonic development.
EEA Balcarce
Fil: Cagnoli, Claudia Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.
Fil: Chiapparrone, María Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.
Fil: Cacciato, Claudio Santiago. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.
Fil: Rodríguez, Marcelo Gastón. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Catena, María del Carmen. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina.
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
description Campylobacter fetus is an important veterinary pathogen that causes campylobacteriosis. This disease causes decreased productivity of cattle by inducing reproductive losses. Although several virulence factors have been recognized in C. fetus, including a cytolethal distending toxin (CDT), the exact mechanism responsible for embryonic death remains unknown. The aim of this work was to evaluate the effect of C. fetus subsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv), and their toxin activity on the in vitro fertilization of bovine ova and early embryonic development. Two different experiments were performed. In Experiment 1, a total of 1524 cumulus–oocyte complexes (COCs) were inseminated, distributed into three groups: two of them infected with the microorganism (Cff, Cfv) and a control group. Percentages COCs cleaved were similar among groups (p = 0.1243); however, the embryonic development rate (blastocyst at day 7) in the control group was greater (p < 0.001) than those obtained in Cff and Cfv groups. In experiment 2, a total of 746 COCs were inseminated, divided into three groups: two of them treated with the bacterial-free culture filtrates to test toxin activity (Cff-CDT, Cfv-CDT) and a control group. Both cleavage and embryonic development rates were greater (p < 0.001) in the control group than those obtained in Cff-CDT and Cfv-CDT groups. This study provides evidence that both subspecies of C. fetus do not interfere with fertilization but do affect in vitro embryonic development. It is the first report on the biological effect of the CDT on bovine embryonic development.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-28T10:43:08Z
2024-02-28T10:43:08Z
2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16814
https://www.sciencedirect.com/science/article/pii/S0378113523002791
1873-2542 (online)
0378-1135 (print)
https://doi.org/10.1016/j.vetmic.2023.109925
url http://hdl.handle.net/20.500.12123/16814
https://www.sciencedirect.com/science/article/pii/S0378113523002791
https://doi.org/10.1016/j.vetmic.2023.109925
identifier_str_mv 1873-2542 (online)
0378-1135 (print)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Microbiology 288 : 109925 (January 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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