Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina
- Autores
- Dorsch, Matias; Regidor-Cerrillo, Javier; Ortega-Mora, Luis; Scioli, Maria Valeria; Morrell, Eleonora Lidia; Canton, German Jose; Moore, Prando Dadin; Hecker, Yanina
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa.
EEA Balcarce
Fil: Dorsch, Matías A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Investigación Agropecuaria (INIA); Uruguay.
Fil: Regidor-Cerrillo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España.
Fil: Ortega-Mora, Luis. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España.
Fil: Scioli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Morrel, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Cantón, Germán Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Moore, Dadín. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Hercker, Yanina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. - Fuente
- Parasitology Research (2020)
- Materia
-
Bovina
Neospora Caninum
Aborto
Microsatélites
Argentina
Bovinae
Abortion
Microsatellites
Región Pampa Húmeda - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/8143
Ver los metadatos del registro completo
id |
INTADig_90af236ec49cbce4ff2993119752549d |
---|---|
oai_identifier_str |
oai:localhost:20.500.12123/8143 |
network_acronym_str |
INTADig |
repository_id_str |
l |
network_name_str |
INTA Digital (INTA) |
spelling |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in ArgentinaDorsch, MatiasRegidor-Cerrillo, JavierOrtega-Mora, LuisScioli, Maria ValeriaMorrell, Eleonora LidiaCanton, German JoseMoore, Prando DadinHecker, YaninaBovinaNeospora CaninumAbortoMicrosatélitesArgentinaBovinaeAbortionMicrosatellitesRegión Pampa HúmedaNeospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa.EEA BalcarceFil: Dorsch, Matías A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Investigación Agropecuaria (INIA); Uruguay.Fil: Regidor-Cerrillo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España.Fil: Ortega-Mora, Luis. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España.Fil: Scioli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Morrel, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Cantón, Germán Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Moore, Dadín. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Hercker, Yanina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Springer Verlag2020-10-28T21:39:08Z2020-10-28T21:39:08Z2020-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8143https://link.springer.com/article/10.1007/s00436-020-06922-x1432-19550932-0113https://doi.org/10.1007/s00436-020-06922-xParasitology Research (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:03Zoai:localhost:20.500.12123/8143instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:03.486INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
title |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
spellingShingle |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina Dorsch, Matias Bovina Neospora Caninum Aborto Microsatélites Argentina Bovinae Abortion Microsatellites Región Pampa Húmeda |
title_short |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
title_full |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
title_fullStr |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
title_full_unstemmed |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
title_sort |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
dc.creator.none.fl_str_mv |
Dorsch, Matias Regidor-Cerrillo, Javier Ortega-Mora, Luis Scioli, Maria Valeria Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Hecker, Yanina |
author |
Dorsch, Matias |
author_facet |
Dorsch, Matias Regidor-Cerrillo, Javier Ortega-Mora, Luis Scioli, Maria Valeria Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Hecker, Yanina |
author_role |
author |
author2 |
Regidor-Cerrillo, Javier Ortega-Mora, Luis Scioli, Maria Valeria Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Hecker, Yanina |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Bovina Neospora Caninum Aborto Microsatélites Argentina Bovinae Abortion Microsatellites Región Pampa Húmeda |
topic |
Bovina Neospora Caninum Aborto Microsatélites Argentina Bovinae Abortion Microsatellites Región Pampa Húmeda |
dc.description.none.fl_txt_mv |
Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. EEA Balcarce Fil: Dorsch, Matías A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Investigación Agropecuaria (INIA); Uruguay. Fil: Regidor-Cerrillo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Ortega-Mora, Luis. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Scioli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Morrel, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Cantón, Germán Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Moore, Dadín. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Hercker, Yanina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. |
description |
Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-28T21:39:08Z 2020-10-28T21:39:08Z 2020-10-15 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/8143 https://link.springer.com/article/10.1007/s00436-020-06922-x 1432-1955 0932-0113 https://doi.org/10.1007/s00436-020-06922-x |
url |
http://hdl.handle.net/20.500.12123/8143 https://link.springer.com/article/10.1007/s00436-020-06922-x https://doi.org/10.1007/s00436-020-06922-x |
identifier_str_mv |
1432-1955 0932-0113 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
Parasitology Research (2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
_version_ |
1844619148436439040 |
score |
12.559606 |