Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies.
- Autores
- Campero, Lucía María; Gual, Ignacio; Dellarupe, Andrea; Schares, Gereon; Moré, Gastón; Moore, Prando Dadin; Venturini, María Cecilia
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Neospora caninum is an important abortifacient agent affecting mainly cattle worldwide. The aim of the present work was to describe the histopathological findings in a naturally infected beef cow and its midterm fetus caused by a genetically defined N. caninum isolate in Argentina. A N. caninum seropositive multiparous Aberdeen Angus pregnant cow and its fetus in the sixth month of gestation were submitted for histopathological, immunohistochemical, serological, and molecular studies and parasite isolation. The cow belonged to a beef herd under extensive management, with a N. caninum seroprevalence of 11%, and low level of annual abortion rate (≤ 5%). The dam had mild lymphocytic infiltrate in CNS, heart and uterus and no parasites were detected by Immunohistochemistry (IHC). No parasitic DNA was detected in the dam's brain, and gamma interferon knockout mice inoculated with brain material did not become infected. Clusters of tachyzoites and parasitic DNA were detected in the placenta by IHC and PCR, respectively. However, isolation from the placenta was unsuccessful. The fetus developed specific antibodies and an inflammatory response was detected in multiple organs. Furthermore, in vitro and in vivo isolation was achieved from gamma interferon knockout mice inoculated with CNS from the fetus. Multilocus-microsatellite typing revealed a genetically defined N. caninum isolate similar to the previously reported as MLG 72. We report the first N. caninum isolate from beef cattle in Argentina.
EEA Balcarce
Fil: Campero, Lucía María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Schares, Gereon. Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Institute of Epidemiology; Alemania.
Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Moore, Dadín Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. - Fuente
- Veterinary Parasitology: Regional Studies and Reports 21 : 100438 (2020)
- Materia
-
Ganado Bovino
Neospora Caninum
Virulencia
Microsatélites
Argentina
Cattle
Virulence
Microsatellites - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/7936
Ver los metadatos del registro completo
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Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies.Campero, Lucía MaríaGual, IgnacioDellarupe, AndreaSchares, GereonMoré, GastónMoore, Prando DadinVenturini, María CeciliaGanado BovinoNeospora CaninumVirulenciaMicrosatélitesArgentinaCattleVirulenceMicrosatellitesNeospora caninum is an important abortifacient agent affecting mainly cattle worldwide. The aim of the present work was to describe the histopathological findings in a naturally infected beef cow and its midterm fetus caused by a genetically defined N. caninum isolate in Argentina. A N. caninum seropositive multiparous Aberdeen Angus pregnant cow and its fetus in the sixth month of gestation were submitted for histopathological, immunohistochemical, serological, and molecular studies and parasite isolation. The cow belonged to a beef herd under extensive management, with a N. caninum seroprevalence of 11%, and low level of annual abortion rate (≤ 5%). The dam had mild lymphocytic infiltrate in CNS, heart and uterus and no parasites were detected by Immunohistochemistry (IHC). No parasitic DNA was detected in the dam's brain, and gamma interferon knockout mice inoculated with brain material did not become infected. Clusters of tachyzoites and parasitic DNA were detected in the placenta by IHC and PCR, respectively. However, isolation from the placenta was unsuccessful. The fetus developed specific antibodies and an inflammatory response was detected in multiple organs. Furthermore, in vitro and in vivo isolation was achieved from gamma interferon knockout mice inoculated with CNS from the fetus. Multilocus-microsatellite typing revealed a genetically defined N. caninum isolate similar to the previously reported as MLG 72. We report the first N. caninum isolate from beef cattle in Argentina.EEA BalcarceFil: Campero, Lucía María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Schares, Gereon. Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Institute of Epidemiology; Alemania.Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Moore, Dadín Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Elsevier2020-09-22T11:57:24Z2020-09-22T11:57:24Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7936https://www.sciencedirect.com/science/article/abs/pii/S24059390203021972405-9390https://doi.org/10.1016/j.vprsr.2020.100438Veterinary Parasitology: Regional Studies and Reports 21 : 100438 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:01Zoai:localhost:20.500.12123/7936instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:01.861INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
title |
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
spellingShingle |
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. Campero, Lucía María Ganado Bovino Neospora Caninum Virulencia Microsatélites Argentina Cattle Virulence Microsatellites |
title_short |
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
title_full |
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
title_fullStr |
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
title_full_unstemmed |
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
title_sort |
Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
dc.creator.none.fl_str_mv |
Campero, Lucía María Gual, Ignacio Dellarupe, Andrea Schares, Gereon Moré, Gastón Moore, Prando Dadin Venturini, María Cecilia |
author |
Campero, Lucía María |
author_facet |
Campero, Lucía María Gual, Ignacio Dellarupe, Andrea Schares, Gereon Moré, Gastón Moore, Prando Dadin Venturini, María Cecilia |
author_role |
author |
author2 |
Gual, Ignacio Dellarupe, Andrea Schares, Gereon Moré, Gastón Moore, Prando Dadin Venturini, María Cecilia |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Ganado Bovino Neospora Caninum Virulencia Microsatélites Argentina Cattle Virulence Microsatellites |
topic |
Ganado Bovino Neospora Caninum Virulencia Microsatélites Argentina Cattle Virulence Microsatellites |
dc.description.none.fl_txt_mv |
Neospora caninum is an important abortifacient agent affecting mainly cattle worldwide. The aim of the present work was to describe the histopathological findings in a naturally infected beef cow and its midterm fetus caused by a genetically defined N. caninum isolate in Argentina. A N. caninum seropositive multiparous Aberdeen Angus pregnant cow and its fetus in the sixth month of gestation were submitted for histopathological, immunohistochemical, serological, and molecular studies and parasite isolation. The cow belonged to a beef herd under extensive management, with a N. caninum seroprevalence of 11%, and low level of annual abortion rate (≤ 5%). The dam had mild lymphocytic infiltrate in CNS, heart and uterus and no parasites were detected by Immunohistochemistry (IHC). No parasitic DNA was detected in the dam's brain, and gamma interferon knockout mice inoculated with brain material did not become infected. Clusters of tachyzoites and parasitic DNA were detected in the placenta by IHC and PCR, respectively. However, isolation from the placenta was unsuccessful. The fetus developed specific antibodies and an inflammatory response was detected in multiple organs. Furthermore, in vitro and in vivo isolation was achieved from gamma interferon knockout mice inoculated with CNS from the fetus. Multilocus-microsatellite typing revealed a genetically defined N. caninum isolate similar to the previously reported as MLG 72. We report the first N. caninum isolate from beef cattle in Argentina. EEA Balcarce Fil: Campero, Lucía María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Schares, Gereon. Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Institute of Epidemiology; Alemania. Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Moore, Dadín Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. |
description |
Neospora caninum is an important abortifacient agent affecting mainly cattle worldwide. The aim of the present work was to describe the histopathological findings in a naturally infected beef cow and its midterm fetus caused by a genetically defined N. caninum isolate in Argentina. A N. caninum seropositive multiparous Aberdeen Angus pregnant cow and its fetus in the sixth month of gestation were submitted for histopathological, immunohistochemical, serological, and molecular studies and parasite isolation. The cow belonged to a beef herd under extensive management, with a N. caninum seroprevalence of 11%, and low level of annual abortion rate (≤ 5%). The dam had mild lymphocytic infiltrate in CNS, heart and uterus and no parasites were detected by Immunohistochemistry (IHC). No parasitic DNA was detected in the dam's brain, and gamma interferon knockout mice inoculated with brain material did not become infected. Clusters of tachyzoites and parasitic DNA were detected in the placenta by IHC and PCR, respectively. However, isolation from the placenta was unsuccessful. The fetus developed specific antibodies and an inflammatory response was detected in multiple organs. Furthermore, in vitro and in vivo isolation was achieved from gamma interferon knockout mice inoculated with CNS from the fetus. Multilocus-microsatellite typing revealed a genetically defined N. caninum isolate similar to the previously reported as MLG 72. We report the first N. caninum isolate from beef cattle in Argentina. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-22T11:57:24Z 2020-09-22T11:57:24Z 2020-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/7936 https://www.sciencedirect.com/science/article/abs/pii/S2405939020302197 2405-9390 https://doi.org/10.1016/j.vprsr.2020.100438 |
url |
http://hdl.handle.net/20.500.12123/7936 https://www.sciencedirect.com/science/article/abs/pii/S2405939020302197 https://doi.org/10.1016/j.vprsr.2020.100438 |
identifier_str_mv |
2405-9390 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Veterinary Parasitology: Regional Studies and Reports 21 : 100438 (2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619147435048960 |
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12.559606 |