Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis

Autores
Regidor Cerrillo, Javier; Díez Fuertes, Francisco; García Culebras, Alicia; Moore, Dadin Prando; González Warleta, Marta; Cuevas, Carmen; Schares, Gereon; Katzer, Frank; Pedraza Diaz, Susana; Mezo, Mercedes; Ortega Mora, Luis M.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cyst-forming protozoan parasite Neospora caninum is one of the main causes of bovine abortion worldwide and is of great economic importance in the cattle industry. Recent studies have revealed extensive genetic variation among N. caninum isolates based on microsatellite sequences (MSs). MSs may be suitable molecular markers for inferring the diversity of parasite populations, molecular epidemiology and the basis for phenotypic variations in N. caninum, which have been poorly defined. In this study, we evaluated nine MS markers using a panel of 11 N. caninum-derived reference isolates from around the world and 96 N. caninum bovine clinical samples and one ovine clinical sample collected from four countries on two continents, including Spain, Argentina, Germany and Scotland, over a 10-year period. These markers were used as molecular tools to investigate the genetic diversity, geographic distribution and population structure of N. caninum. Multilocus microsatellite genotyping based on 7 loci demonstrated high levels of genetic diversity in the samples from all of the different countries, with 96 microsatellite multilocus genotypes (MLGs) identified from 108 N. caninum samples. Geographic sub-structuring was present in the country populations according to pairwise FST. Principal component analysis (PCA) and Neighbor Joining tree topologies also suggested MLG segregation partially associated with geographical origin. An analysis of the MLG relationships, using eBURST, confirmed that the close genetic relationship observed between the Spanish and Argentinean populations may be the result of parasite migration (i.e., the introduction of novel MLGs from Spain to South America) due to cattle movement. The eBURST relationships also revealed genetically different clusters associated with the abortion. The presence of linkage disequilibrium, the co-existence of specific MLGs to individual farms and eBURST MLG relationships suggest a predominant clonal propagation for Spanish N. caninum MLGs in cattle.
Fil: Regidor Cerrillo, Javier. Universidad Complutense de Madrid; España
Fil: Díez Fuertes, Francisco. Universidad Complutense de Madrid; España
Fil: García Culebras, Alicia. Universidad Complutense de Madrid; España
Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González Warleta, Marta. Agricultural Research Center of Mabegondo; España
Fil: Cuevas, Carmen. Universidad Complutense de Madrid; España
Fil: Schares, Gereon. Agricultural Research Center of Mabegondo; España
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Pedraza Diaz, Susana. Universidad Complutense de Madrid; España
Fil: Mezo, Mercedes. Agricultural Research Center of Mabegondo; España
Fil: Ortega Mora, Luis M.. Universidad Complutense de Madrid; España
Materia
Genetic diversity
Neospora caninum
Cattle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25122
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25122
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping AnalysisRegidor Cerrillo, JavierDíez Fuertes, FranciscoGarcía Culebras, AliciaMoore, Dadin PrandoGonzález Warleta, MartaCuevas, CarmenSchares, GereonKatzer, FrankPedraza Diaz, SusanaMezo, MercedesOrtega Mora, Luis M.Genetic diversityNeospora caninumCattleThe cyst-forming protozoan parasite Neospora caninum is one of the main causes of bovine abortion worldwide and is of great economic importance in the cattle industry. Recent studies have revealed extensive genetic variation among N. caninum isolates based on microsatellite sequences (MSs). MSs may be suitable molecular markers for inferring the diversity of parasite populations, molecular epidemiology and the basis for phenotypic variations in N. caninum, which have been poorly defined. In this study, we evaluated nine MS markers using a panel of 11 N. caninum-derived reference isolates from around the world and 96 N. caninum bovine clinical samples and one ovine clinical sample collected from four countries on two continents, including Spain, Argentina, Germany and Scotland, over a 10-year period. These markers were used as molecular tools to investigate the genetic diversity, geographic distribution and population structure of N. caninum. Multilocus microsatellite genotyping based on 7 loci demonstrated high levels of genetic diversity in the samples from all of the different countries, with 96 microsatellite multilocus genotypes (MLGs) identified from 108 N. caninum samples. Geographic sub-structuring was present in the country populations according to pairwise FST. Principal component analysis (PCA) and Neighbor Joining tree topologies also suggested MLG segregation partially associated with geographical origin. An analysis of the MLG relationships, using eBURST, confirmed that the close genetic relationship observed between the Spanish and Argentinean populations may be the result of parasite migration (i.e., the introduction of novel MLGs from Spain to South America) due to cattle movement. The eBURST relationships also revealed genetically different clusters associated with the abortion. The presence of linkage disequilibrium, the co-existence of specific MLGs to individual farms and eBURST MLG relationships suggest a predominant clonal propagation for Spanish N. caninum MLGs in cattle.Fil: Regidor Cerrillo, Javier. Universidad Complutense de Madrid; EspañaFil: Díez Fuertes, Francisco. Universidad Complutense de Madrid; EspañaFil: García Culebras, Alicia. Universidad Complutense de Madrid; EspañaFil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: González Warleta, Marta. Agricultural Research Center of Mabegondo; EspañaFil: Cuevas, Carmen. Universidad Complutense de Madrid; EspañaFil: Schares, Gereon. Agricultural Research Center of Mabegondo; EspañaFil: Katzer, Frank. Moredun Research Institute; Reino UnidoFil: Pedraza Diaz, Susana. Universidad Complutense de Madrid; EspañaFil: Mezo, Mercedes. Agricultural Research Center of Mabegondo; EspañaFil: Ortega Mora, Luis M.. Universidad Complutense de Madrid; EspañaPublic Library of Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25122Regidor Cerrillo, Javier; Díez Fuertes, Francisco; García Culebras, Alicia; Moore, Dadin Prando; González Warleta, Marta; et al.; Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis; Public Library of Science; Plos One; 8; 8; 8-2013; e7267801-e72678121932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072678info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0072678info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:38Zoai:ri.conicet.gov.ar:11336/25122instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:38.782CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis
title Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis
spellingShingle Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis
Regidor Cerrillo, Javier
Genetic diversity
Neospora caninum
Cattle
title_short Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis
title_full Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis
title_fullStr Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis
title_full_unstemmed Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis
title_sort Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis
dc.creator.none.fl_str_mv Regidor Cerrillo, Javier
Díez Fuertes, Francisco
García Culebras, Alicia
Moore, Dadin Prando
González Warleta, Marta
Cuevas, Carmen
Schares, Gereon
Katzer, Frank
Pedraza Diaz, Susana
Mezo, Mercedes
Ortega Mora, Luis M.
author Regidor Cerrillo, Javier
author_facet Regidor Cerrillo, Javier
Díez Fuertes, Francisco
García Culebras, Alicia
Moore, Dadin Prando
González Warleta, Marta
Cuevas, Carmen
Schares, Gereon
Katzer, Frank
Pedraza Diaz, Susana
Mezo, Mercedes
Ortega Mora, Luis M.
author_role author
author2 Díez Fuertes, Francisco
García Culebras, Alicia
Moore, Dadin Prando
González Warleta, Marta
Cuevas, Carmen
Schares, Gereon
Katzer, Frank
Pedraza Diaz, Susana
Mezo, Mercedes
Ortega Mora, Luis M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genetic diversity
Neospora caninum
Cattle
topic Genetic diversity
Neospora caninum
Cattle
dc.description.none.fl_txt_mv The cyst-forming protozoan parasite Neospora caninum is one of the main causes of bovine abortion worldwide and is of great economic importance in the cattle industry. Recent studies have revealed extensive genetic variation among N. caninum isolates based on microsatellite sequences (MSs). MSs may be suitable molecular markers for inferring the diversity of parasite populations, molecular epidemiology and the basis for phenotypic variations in N. caninum, which have been poorly defined. In this study, we evaluated nine MS markers using a panel of 11 N. caninum-derived reference isolates from around the world and 96 N. caninum bovine clinical samples and one ovine clinical sample collected from four countries on two continents, including Spain, Argentina, Germany and Scotland, over a 10-year period. These markers were used as molecular tools to investigate the genetic diversity, geographic distribution and population structure of N. caninum. Multilocus microsatellite genotyping based on 7 loci demonstrated high levels of genetic diversity in the samples from all of the different countries, with 96 microsatellite multilocus genotypes (MLGs) identified from 108 N. caninum samples. Geographic sub-structuring was present in the country populations according to pairwise FST. Principal component analysis (PCA) and Neighbor Joining tree topologies also suggested MLG segregation partially associated with geographical origin. An analysis of the MLG relationships, using eBURST, confirmed that the close genetic relationship observed between the Spanish and Argentinean populations may be the result of parasite migration (i.e., the introduction of novel MLGs from Spain to South America) due to cattle movement. The eBURST relationships also revealed genetically different clusters associated with the abortion. The presence of linkage disequilibrium, the co-existence of specific MLGs to individual farms and eBURST MLG relationships suggest a predominant clonal propagation for Spanish N. caninum MLGs in cattle.
Fil: Regidor Cerrillo, Javier. Universidad Complutense de Madrid; España
Fil: Díez Fuertes, Francisco. Universidad Complutense de Madrid; España
Fil: García Culebras, Alicia. Universidad Complutense de Madrid; España
Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González Warleta, Marta. Agricultural Research Center of Mabegondo; España
Fil: Cuevas, Carmen. Universidad Complutense de Madrid; España
Fil: Schares, Gereon. Agricultural Research Center of Mabegondo; España
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Pedraza Diaz, Susana. Universidad Complutense de Madrid; España
Fil: Mezo, Mercedes. Agricultural Research Center of Mabegondo; España
Fil: Ortega Mora, Luis M.. Universidad Complutense de Madrid; España
description The cyst-forming protozoan parasite Neospora caninum is one of the main causes of bovine abortion worldwide and is of great economic importance in the cattle industry. Recent studies have revealed extensive genetic variation among N. caninum isolates based on microsatellite sequences (MSs). MSs may be suitable molecular markers for inferring the diversity of parasite populations, molecular epidemiology and the basis for phenotypic variations in N. caninum, which have been poorly defined. In this study, we evaluated nine MS markers using a panel of 11 N. caninum-derived reference isolates from around the world and 96 N. caninum bovine clinical samples and one ovine clinical sample collected from four countries on two continents, including Spain, Argentina, Germany and Scotland, over a 10-year period. These markers were used as molecular tools to investigate the genetic diversity, geographic distribution and population structure of N. caninum. Multilocus microsatellite genotyping based on 7 loci demonstrated high levels of genetic diversity in the samples from all of the different countries, with 96 microsatellite multilocus genotypes (MLGs) identified from 108 N. caninum samples. Geographic sub-structuring was present in the country populations according to pairwise FST. Principal component analysis (PCA) and Neighbor Joining tree topologies also suggested MLG segregation partially associated with geographical origin. An analysis of the MLG relationships, using eBURST, confirmed that the close genetic relationship observed between the Spanish and Argentinean populations may be the result of parasite migration (i.e., the introduction of novel MLGs from Spain to South America) due to cattle movement. The eBURST relationships also revealed genetically different clusters associated with the abortion. The presence of linkage disequilibrium, the co-existence of specific MLGs to individual farms and eBURST MLG relationships suggest a predominant clonal propagation for Spanish N. caninum MLGs in cattle.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25122
Regidor Cerrillo, Javier; Díez Fuertes, Francisco; García Culebras, Alicia; Moore, Dadin Prando; González Warleta, Marta; et al.; Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis; Public Library of Science; Plos One; 8; 8; 8-2013; e7267801-e7267812
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25122
identifier_str_mv Regidor Cerrillo, Javier; Díez Fuertes, Francisco; García Culebras, Alicia; Moore, Dadin Prando; González Warleta, Marta; et al.; Genetic Diversity and Geographic Population Structure of Bovine Neospora caninum Determined by Microsatellite Genotyping Analysis; Public Library of Science; Plos One; 8; 8; 8-2013; e7267801-e7267812
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072678
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0072678
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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