The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes

Autores
Kashiwa, Takeshi; Lavilla, Miguel Angel; Diaz Paleo, Antonio Horacio; Ivancovich, Antonio Juan Gerardo; Yamanaka, Naoki
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cercospora leaf blight (CLB) causes extensive losses in soybean production in worldwide, including major soybean-producing countries such as Argentina. Cercospora kikuchii, C. cf. sigesbeckiae, C. cf. flagellaris, and C. cf. nicotianae are identified as pathogens of CLB. Soybean resistance against CLB is still unknown. Also, chemical control for CLB is losing effectiveness because of fungicide resistance of pathogens such as C. kikuchii. We urgently need to breed a CLB-resistant cultivar. Unfortunately, efficient methods for the screening of a resistant soybean genotype have not yet been established. In this study, we designed a new, high-throughput inoculation method for identifying resistance against one of the CLB pathogens, C. kikuchii. We used liquid-cultured mycelia of the pathogen C. kikuchii on detached soybean leaves. Lesions on soybean leaflets appeared 9 days postinoculation by this method. We used this method to select four C. kikuchii-resistant genotypes from 80 genotypes in the World Soybean Core Collection. The high-throughput screening method developed in this study can contribute to the research about C. kikuchii resistance by facilitating identification of resistant varieties.
EEA Pergamino
Fil: Kashiwa, Takeshi. Japan International Research Center for Agricultural Sciences (JIRCAS). Biological Resources and Post-harvest Division; Japón
Fil: Lavilla, Miguel Ángel. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; Argentina
Fil: Diaz Paleo, Antonio Horacio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Ivancovich, Antonio Juan Gerardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Yamanaka, Naoki. Japan International Research Center for Agricultural Sciences (JIRCAS). Biological Resources and Post-harvest Division; Japón
Fuente
PhytoFrontiers 1 (4) : 250-257. (November 2021)
Materia
Soja
Inoculación
Métodos de Aplicación
Genotipos
Cercospora kikuchii
Organismos Patógenos
Soybeans
Inoculation
Application Methods
Genotypes
Pathogens
Cercospora Leaf Blight
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11162
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11162
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean GenotypesKashiwa, TakeshiLavilla, Miguel AngelDiaz Paleo, Antonio HoracioIvancovich, Antonio Juan GerardoYamanaka, NaokiSojaInoculaciónMétodos de AplicaciónGenotiposCercospora kikuchiiOrganismos PatógenosSoybeansInoculationApplication MethodsGenotypesPathogensCercospora Leaf BlightCercospora leaf blight (CLB) causes extensive losses in soybean production in worldwide, including major soybean-producing countries such as Argentina. Cercospora kikuchii, C. cf. sigesbeckiae, C. cf. flagellaris, and C. cf. nicotianae are identified as pathogens of CLB. Soybean resistance against CLB is still unknown. Also, chemical control for CLB is losing effectiveness because of fungicide resistance of pathogens such as C. kikuchii. We urgently need to breed a CLB-resistant cultivar. Unfortunately, efficient methods for the screening of a resistant soybean genotype have not yet been established. In this study, we designed a new, high-throughput inoculation method for identifying resistance against one of the CLB pathogens, C. kikuchii. We used liquid-cultured mycelia of the pathogen C. kikuchii on detached soybean leaves. Lesions on soybean leaflets appeared 9 days postinoculation by this method. We used this method to select four C. kikuchii-resistant genotypes from 80 genotypes in the World Soybean Core Collection. The high-throughput screening method developed in this study can contribute to the research about C. kikuchii resistance by facilitating identification of resistant varieties.EEA PergaminoFil: Kashiwa, Takeshi. Japan International Research Center for Agricultural Sciences (JIRCAS). Biological Resources and Post-harvest Division; JapónFil: Lavilla, Miguel Ángel. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; ArgentinaFil: Diaz Paleo, Antonio Horacio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Ivancovich, Antonio Juan Gerardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Yamanaka, Naoki. Japan International Research Center for Agricultural Sciences (JIRCAS). Biological Resources and Post-harvest Division; JapónThe American Phytopathological Society (APS)2022-02-16T11:25:11Z2022-02-16T11:25:11Z2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11162https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-01-21-0002-TA2690-5442https://doi.org/10.1094/PHYTOFR-01-21-0002-TAPhytoFrontiers 1 (4) : 250-257. (November 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:28Zoai:localhost:20.500.12123/11162instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:29.23INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
title The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
spellingShingle The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
Kashiwa, Takeshi
Soja
Inoculación
Métodos de Aplicación
Genotipos
Cercospora kikuchii
Organismos Patógenos
Soybeans
Inoculation
Application Methods
Genotypes
Pathogens
Cercospora Leaf Blight
title_short The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
title_full The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
title_fullStr The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
title_full_unstemmed The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
title_sort The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
dc.creator.none.fl_str_mv Kashiwa, Takeshi
Lavilla, Miguel Angel
Diaz Paleo, Antonio Horacio
Ivancovich, Antonio Juan Gerardo
Yamanaka, Naoki
author Kashiwa, Takeshi
author_facet Kashiwa, Takeshi
Lavilla, Miguel Angel
Diaz Paleo, Antonio Horacio
Ivancovich, Antonio Juan Gerardo
Yamanaka, Naoki
author_role author
author2 Lavilla, Miguel Angel
Diaz Paleo, Antonio Horacio
Ivancovich, Antonio Juan Gerardo
Yamanaka, Naoki
author2_role author
author
author
author
dc.subject.none.fl_str_mv Soja
Inoculación
Métodos de Aplicación
Genotipos
Cercospora kikuchii
Organismos Patógenos
Soybeans
Inoculation
Application Methods
Genotypes
Pathogens
Cercospora Leaf Blight
topic Soja
Inoculación
Métodos de Aplicación
Genotipos
Cercospora kikuchii
Organismos Patógenos
Soybeans
Inoculation
Application Methods
Genotypes
Pathogens
Cercospora Leaf Blight
dc.description.none.fl_txt_mv Cercospora leaf blight (CLB) causes extensive losses in soybean production in worldwide, including major soybean-producing countries such as Argentina. Cercospora kikuchii, C. cf. sigesbeckiae, C. cf. flagellaris, and C. cf. nicotianae are identified as pathogens of CLB. Soybean resistance against CLB is still unknown. Also, chemical control for CLB is losing effectiveness because of fungicide resistance of pathogens such as C. kikuchii. We urgently need to breed a CLB-resistant cultivar. Unfortunately, efficient methods for the screening of a resistant soybean genotype have not yet been established. In this study, we designed a new, high-throughput inoculation method for identifying resistance against one of the CLB pathogens, C. kikuchii. We used liquid-cultured mycelia of the pathogen C. kikuchii on detached soybean leaves. Lesions on soybean leaflets appeared 9 days postinoculation by this method. We used this method to select four C. kikuchii-resistant genotypes from 80 genotypes in the World Soybean Core Collection. The high-throughput screening method developed in this study can contribute to the research about C. kikuchii resistance by facilitating identification of resistant varieties.
EEA Pergamino
Fil: Kashiwa, Takeshi. Japan International Research Center for Agricultural Sciences (JIRCAS). Biological Resources and Post-harvest Division; Japón
Fil: Lavilla, Miguel Ángel. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; Argentina
Fil: Diaz Paleo, Antonio Horacio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Ivancovich, Antonio Juan Gerardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Yamanaka, Naoki. Japan International Research Center for Agricultural Sciences (JIRCAS). Biological Resources and Post-harvest Division; Japón
description Cercospora leaf blight (CLB) causes extensive losses in soybean production in worldwide, including major soybean-producing countries such as Argentina. Cercospora kikuchii, C. cf. sigesbeckiae, C. cf. flagellaris, and C. cf. nicotianae are identified as pathogens of CLB. Soybean resistance against CLB is still unknown. Also, chemical control for CLB is losing effectiveness because of fungicide resistance of pathogens such as C. kikuchii. We urgently need to breed a CLB-resistant cultivar. Unfortunately, efficient methods for the screening of a resistant soybean genotype have not yet been established. In this study, we designed a new, high-throughput inoculation method for identifying resistance against one of the CLB pathogens, C. kikuchii. We used liquid-cultured mycelia of the pathogen C. kikuchii on detached soybean leaves. Lesions on soybean leaflets appeared 9 days postinoculation by this method. We used this method to select four C. kikuchii-resistant genotypes from 80 genotypes in the World Soybean Core Collection. The high-throughput screening method developed in this study can contribute to the research about C. kikuchii resistance by facilitating identification of resistant varieties.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
2022-02-16T11:25:11Z
2022-02-16T11:25:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11162
https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-01-21-0002-TA
2690-5442
https://doi.org/10.1094/PHYTOFR-01-21-0002-TA
url http://hdl.handle.net/20.500.12123/11162
https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-01-21-0002-TA
https://doi.org/10.1094/PHYTOFR-01-21-0002-TA
identifier_str_mv 2690-5442
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The American Phytopathological Society (APS)
publisher.none.fl_str_mv The American Phytopathological Society (APS)
dc.source.none.fl_str_mv PhytoFrontiers 1 (4) : 250-257. (November 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619161938952192
score 12.559606

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