Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods

Autores
Latorre Rapela, María Gabriela de Los Milagros; Lura, Maria Cristina Enriqueta; Marcipar, Iván Sergio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Late-cycle diseases (LCD) cause a significant deterioration in quality and reduce yields in soybean crops. In Argentina, in particular, leaf blight and purple seed stain, caused by the agent Cercospora kikuchii, and frog eye spot, caused by C. sojina, are the prevailing sources of diseases. The early,rapid and accurate detection of these phytopathogens becomes essential, and would contribute to preserving both the environment and the health of humans and animals by preventing the wasteful or improper use of chemicals such as pesticides. In order to detect Cercospora species in soybean plants at an early stage, immunochemical and molecular techniques were developed in this work. Strains from the NITE Biological Resource Center collection (Japan): Cercospora kikuchii NBRC 6711 and Cercospora sojina NBRC 6715 and regional isolates of C. kikuchii were used. To develop Dot-Blot and PCR techniques, experiments with plants undergoing different treatmentswere carried out: those experimentally inoculated with these fungi, those treated with sterile water and healthy plants as well. Both techniques allowed the detection, at early stages, of Cercospora species involved in two of the most frequent LCD in the country, when the cercosporin concentration produced by the fungus was higher than 3.93 ± 0.39 nmol∙cyl−1 ±SD. The sensitivity between both techniques was very different. While Dot-Blot allowed the detection of the disease 4 days after inoculation, PCR detected it after 4 hours, even without visible symptoms of the disease.
Fil: Latorre Rapela, María Gabriela de Los Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Lura, Maria Cristina Enriqueta. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Marcipar, Iván Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
CERCOSPORA KIKUCHII
CERCOSPORA SOJINA
DOT-BLOT
PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41364
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular MethodsLatorre Rapela, María Gabriela de Los MilagrosLura, Maria Cristina EnriquetaMarcipar, Iván SergioCERCOSPORA KIKUCHIICERCOSPORA SOJINADOT-BLOTPCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Late-cycle diseases (LCD) cause a significant deterioration in quality and reduce yields in soybean crops. In Argentina, in particular, leaf blight and purple seed stain, caused by the agent Cercospora kikuchii, and frog eye spot, caused by C. sojina, are the prevailing sources of diseases. The early,rapid and accurate detection of these phytopathogens becomes essential, and would contribute to preserving both the environment and the health of humans and animals by preventing the wasteful or improper use of chemicals such as pesticides. In order to detect Cercospora species in soybean plants at an early stage, immunochemical and molecular techniques were developed in this work. Strains from the NITE Biological Resource Center collection (Japan): Cercospora kikuchii NBRC 6711 and Cercospora sojina NBRC 6715 and regional isolates of C. kikuchii were used. To develop Dot-Blot and PCR techniques, experiments with plants undergoing different treatmentswere carried out: those experimentally inoculated with these fungi, those treated with sterile water and healthy plants as well. Both techniques allowed the detection, at early stages, of Cercospora species involved in two of the most frequent LCD in the country, when the cercosporin concentration produced by the fungus was higher than 3.93 ± 0.39 nmol∙cyl−1 ±SD. The sensitivity between both techniques was very different. While Dot-Blot allowed the detection of the disease 4 days after inoculation, PCR detected it after 4 hours, even without visible symptoms of the disease.Fil: Latorre Rapela, María Gabriela de Los Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Lura, Maria Cristina Enriqueta. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Marcipar, Iván Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaScientific Research Publishing2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41364Latorre Rapela, María Gabriela de Los Milagros; Lura, Maria Cristina Enriqueta; Marcipar, Iván Sergio; Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods; Scientific Research Publishing; American Journal of Plant Sciences; 6; 18; 11-2015; 2939-29482158-27422158-2750CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4236/ajps.2015.618289info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/15-2602280_61439.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:57Zoai:ri.conicet.gov.ar:11336/41364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:57.755CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods
title Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods
spellingShingle Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods
Latorre Rapela, María Gabriela de Los Milagros
CERCOSPORA KIKUCHII
CERCOSPORA SOJINA
DOT-BLOT
PCR
title_short Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods
title_full Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods
title_fullStr Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods
title_full_unstemmed Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods
title_sort Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods
dc.creator.none.fl_str_mv Latorre Rapela, María Gabriela de Los Milagros
Lura, Maria Cristina Enriqueta
Marcipar, Iván Sergio
author Latorre Rapela, María Gabriela de Los Milagros
author_facet Latorre Rapela, María Gabriela de Los Milagros
Lura, Maria Cristina Enriqueta
Marcipar, Iván Sergio
author_role author
author2 Lura, Maria Cristina Enriqueta
Marcipar, Iván Sergio
author2_role author
author
dc.subject.none.fl_str_mv CERCOSPORA KIKUCHII
CERCOSPORA SOJINA
DOT-BLOT
PCR
topic CERCOSPORA KIKUCHII
CERCOSPORA SOJINA
DOT-BLOT
PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Late-cycle diseases (LCD) cause a significant deterioration in quality and reduce yields in soybean crops. In Argentina, in particular, leaf blight and purple seed stain, caused by the agent Cercospora kikuchii, and frog eye spot, caused by C. sojina, are the prevailing sources of diseases. The early,rapid and accurate detection of these phytopathogens becomes essential, and would contribute to preserving both the environment and the health of humans and animals by preventing the wasteful or improper use of chemicals such as pesticides. In order to detect Cercospora species in soybean plants at an early stage, immunochemical and molecular techniques were developed in this work. Strains from the NITE Biological Resource Center collection (Japan): Cercospora kikuchii NBRC 6711 and Cercospora sojina NBRC 6715 and regional isolates of C. kikuchii were used. To develop Dot-Blot and PCR techniques, experiments with plants undergoing different treatmentswere carried out: those experimentally inoculated with these fungi, those treated with sterile water and healthy plants as well. Both techniques allowed the detection, at early stages, of Cercospora species involved in two of the most frequent LCD in the country, when the cercosporin concentration produced by the fungus was higher than 3.93 ± 0.39 nmol∙cyl−1 ±SD. The sensitivity between both techniques was very different. While Dot-Blot allowed the detection of the disease 4 days after inoculation, PCR detected it after 4 hours, even without visible symptoms of the disease.
Fil: Latorre Rapela, María Gabriela de Los Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Lura, Maria Cristina Enriqueta. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Marcipar, Iván Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description Late-cycle diseases (LCD) cause a significant deterioration in quality and reduce yields in soybean crops. In Argentina, in particular, leaf blight and purple seed stain, caused by the agent Cercospora kikuchii, and frog eye spot, caused by C. sojina, are the prevailing sources of diseases. The early,rapid and accurate detection of these phytopathogens becomes essential, and would contribute to preserving both the environment and the health of humans and animals by preventing the wasteful or improper use of chemicals such as pesticides. In order to detect Cercospora species in soybean plants at an early stage, immunochemical and molecular techniques were developed in this work. Strains from the NITE Biological Resource Center collection (Japan): Cercospora kikuchii NBRC 6711 and Cercospora sojina NBRC 6715 and regional isolates of C. kikuchii were used. To develop Dot-Blot and PCR techniques, experiments with plants undergoing different treatmentswere carried out: those experimentally inoculated with these fungi, those treated with sterile water and healthy plants as well. Both techniques allowed the detection, at early stages, of Cercospora species involved in two of the most frequent LCD in the country, when the cercosporin concentration produced by the fungus was higher than 3.93 ± 0.39 nmol∙cyl−1 ±SD. The sensitivity between both techniques was very different. While Dot-Blot allowed the detection of the disease 4 days after inoculation, PCR detected it after 4 hours, even without visible symptoms of the disease.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41364
Latorre Rapela, María Gabriela de Los Milagros; Lura, Maria Cristina Enriqueta; Marcipar, Iván Sergio; Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods; Scientific Research Publishing; American Journal of Plant Sciences; 6; 18; 11-2015; 2939-2948
2158-2742
2158-2750
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41364
identifier_str_mv Latorre Rapela, María Gabriela de Los Milagros; Lura, Maria Cristina Enriqueta; Marcipar, Iván Sergio; Early Detection of Cercospora Species in Soybean Plants: Immunologic and Molecular Methods; Scientific Research Publishing; American Journal of Plant Sciences; 6; 18; 11-2015; 2939-2948
2158-2742
2158-2750
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/ajps.2015.618289
info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/15-2602280_61439.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research Publishing
publisher.none.fl_str_mv Scientific Research Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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