Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat

Autores
Bossio, Adrian Ezequiel; Diaz Paleo, Antonio Horacio; Del Vas, Mariana; Baroli, Irene; Acevedo, Alberto; Rios, Raul Daniel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the influence of post-transcriptional silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic embryogenesis in wheat. We found that PTGS of either of the target genes drastically inhibits callus regeneration and overall efficiency of transformation, in a similar manner as the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the medium with glutathione did not overcome the observed low efficiency of wheat transformation. Furthermore, of the small number of obtained transformants, none exhibited altered GSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential for somatic embryogenesis and, as a consequence, it is difficult to regenerate wheat plants with silenced GSH1 and GSH2 genes.
Instituto de Genética
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación de Recursos Naturales; Argentina
Fil: Ríos, Raúl Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fuente
Plant Cell, Tissue and Organ Culture 112 (2) : 239–248 (February 2013)
Materia
Embriogénesis Somática
Trigo
Triticum aestivum
Biosíntesis
Glutation
Transcripción Génica
Somatic Embryogenesis
Wheat
Bioshyntesis
Glutathione
Gene Transcription
Silenciamiento postranscripcional
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3432
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3432
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network_name_str INTA Digital (INTA)
spelling Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheatBossio, Adrian EzequielDiaz Paleo, Antonio HoracioDel Vas, MarianaBaroli, IreneAcevedo, AlbertoRios, Raul DanielEmbriogénesis SomáticaTrigoTriticum aestivumBiosíntesisGlutationTranscripción GénicaSomatic EmbryogenesisWheatBioshyntesisGlutathioneGene TranscriptionSilenciamiento postranscripcionalSomatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the influence of post-transcriptional silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic embryogenesis in wheat. We found that PTGS of either of the target genes drastically inhibits callus regeneration and overall efficiency of transformation, in a similar manner as the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the medium with glutathione did not overcome the observed low efficiency of wheat transformation. Furthermore, of the small number of obtained transformants, none exhibited altered GSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential for somatic embryogenesis and, as a consequence, it is difficult to regenerate wheat plants with silenced GSH1 and GSH2 genes.Instituto de GenéticaFil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.Fil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación de Recursos Naturales; ArgentinaFil: Ríos, Raúl Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaSpringer2018-09-20T17:25:42Z2018-09-20T17:25:42Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3432https://link.springer.com/article/10.1007/s11240-012-0228-41573-5044https://doi.org/10.1007/s11240-012-0228-4Plant Cell, Tissue and Organ Culture 112 (2) : 239–248 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:26Zoai:localhost:20.500.12123/3432instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:26.875INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
title Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
spellingShingle Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
Bossio, Adrian Ezequiel
Embriogénesis Somática
Trigo
Triticum aestivum
Biosíntesis
Glutation
Transcripción Génica
Somatic Embryogenesis
Wheat
Bioshyntesis
Glutathione
Gene Transcription
Silenciamiento postranscripcional
title_short Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
title_full Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
title_fullStr Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
title_full_unstemmed Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
title_sort Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
dc.creator.none.fl_str_mv Bossio, Adrian Ezequiel
Diaz Paleo, Antonio Horacio
Del Vas, Mariana
Baroli, Irene
Acevedo, Alberto
Rios, Raul Daniel
author Bossio, Adrian Ezequiel
author_facet Bossio, Adrian Ezequiel
Diaz Paleo, Antonio Horacio
Del Vas, Mariana
Baroli, Irene
Acevedo, Alberto
Rios, Raul Daniel
author_role author
author2 Diaz Paleo, Antonio Horacio
Del Vas, Mariana
Baroli, Irene
Acevedo, Alberto
Rios, Raul Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Embriogénesis Somática
Trigo
Triticum aestivum
Biosíntesis
Glutation
Transcripción Génica
Somatic Embryogenesis
Wheat
Bioshyntesis
Glutathione
Gene Transcription
Silenciamiento postranscripcional
topic Embriogénesis Somática
Trigo
Triticum aestivum
Biosíntesis
Glutation
Transcripción Génica
Somatic Embryogenesis
Wheat
Bioshyntesis
Glutathione
Gene Transcription
Silenciamiento postranscripcional
dc.description.none.fl_txt_mv Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the influence of post-transcriptional silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic embryogenesis in wheat. We found that PTGS of either of the target genes drastically inhibits callus regeneration and overall efficiency of transformation, in a similar manner as the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the medium with glutathione did not overcome the observed low efficiency of wheat transformation. Furthermore, of the small number of obtained transformants, none exhibited altered GSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential for somatic embryogenesis and, as a consequence, it is difficult to regenerate wheat plants with silenced GSH1 and GSH2 genes.
Instituto de Genética
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación de Recursos Naturales; Argentina
Fil: Ríos, Raúl Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
description Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the influence of post-transcriptional silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic embryogenesis in wheat. We found that PTGS of either of the target genes drastically inhibits callus regeneration and overall efficiency of transformation, in a similar manner as the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the medium with glutathione did not overcome the observed low efficiency of wheat transformation. Furthermore, of the small number of obtained transformants, none exhibited altered GSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential for somatic embryogenesis and, as a consequence, it is difficult to regenerate wheat plants with silenced GSH1 and GSH2 genes.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
2018-09-20T17:25:42Z
2018-09-20T17:25:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3432
https://link.springer.com/article/10.1007/s11240-012-0228-4
1573-5044
https://doi.org/10.1007/s11240-012-0228-4
url http://hdl.handle.net/20.500.12123/3432
https://link.springer.com/article/10.1007/s11240-012-0228-4
https://doi.org/10.1007/s11240-012-0228-4
identifier_str_mv 1573-5044
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Plant Cell, Tissue and Organ Culture 112 (2) : 239–248 (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619126329311232
score 12.558318

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