High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)

Autores
Goytia Bertero, Valentina; Beznec, Ailin; Faccio, Paula Daniela; Auteri, Micol Tais; Arteaga, Martin; Bonafede, Marcos; Bossio, Maria Emilia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for “peperina” plants (Minthostachys verticillata (Griseb.) Epling). In vitro shoots developed via shoot apex extracted from greenhouse-grown plants were cultured on shoot elongation medium (ShM) consisting of Murashige and Skoog (MS) basal salts supplemented with myoinositol, thiamine, and benzyladenine. Shoot apexes were disinfected with 70% ethanol for 5 min and 0.26 sodium hypochlorite (w/v) for 5 min, before the initiation of in vitro culture. For somatic embryo (SE) induction, leaves collected from 2-mo-old in vitro raised shoots were cultured on MS basal medium supplemented with benzyladenine and two different concentrations of coconut water (CW) until SE developed. Using 2.5% CW-supplemented medium, 100% of cultured leaves developed SE and 89.3% of the leaf explants developed plantlets. The resulting embryos germinated on the same medium, and the plantlets obtained were transferred onto ShM until they developed 3 to 4 leaves. To increase the low root development, shoots were transferred into fully hydrated perlite for rooting and then transplanted into soil-perlite containing pots for hardening. This protocol provides the first step to supply the demand and simultaneously protect the natural populations of Minthostachys verticillata from overexploitation. Furthermore, this protocol provides the first step for crop improvement by genetic modification (editing or transgenesis).
Fil: Goytia Bertero, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas Químicas y Naturales. Cátedra de Ingeniería Genética; Argentina
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Arteaga, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Auteri, Micol Tais. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Ingeniería Genética; Argentina
Fuente
In Vitro Cellular & Developmental Biology - Plant (2020)
Materia
Minthostachys
Somatic Embryogenesis
In Vitro Culture
Embriogénesis Somática
Cultivo in Vitro
Griseb
Peperina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8145
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8145
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spelling High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)Goytia Bertero, ValentinaBeznec, AilinFaccio, Paula DanielaAuteri, Micol TaisArteaga, MartinBonafede, MarcosBossio, Maria EmiliaMinthostachysSomatic EmbryogenesisIn Vitro CultureEmbriogénesis SomáticaCultivo in VitroGrisebPeperinaIn vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for “peperina” plants (Minthostachys verticillata (Griseb.) Epling). In vitro shoots developed via shoot apex extracted from greenhouse-grown plants were cultured on shoot elongation medium (ShM) consisting of Murashige and Skoog (MS) basal salts supplemented with myoinositol, thiamine, and benzyladenine. Shoot apexes were disinfected with 70% ethanol for 5 min and 0.26 sodium hypochlorite (w/v) for 5 min, before the initiation of in vitro culture. For somatic embryo (SE) induction, leaves collected from 2-mo-old in vitro raised shoots were cultured on MS basal medium supplemented with benzyladenine and two different concentrations of coconut water (CW) until SE developed. Using 2.5% CW-supplemented medium, 100% of cultured leaves developed SE and 89.3% of the leaf explants developed plantlets. The resulting embryos germinated on the same medium, and the plantlets obtained were transferred onto ShM until they developed 3 to 4 leaves. To increase the low root development, shoots were transferred into fully hydrated perlite for rooting and then transplanted into soil-perlite containing pots for hardening. This protocol provides the first step to supply the demand and simultaneously protect the natural populations of Minthostachys verticillata from overexploitation. Furthermore, this protocol provides the first step for crop improvement by genetic modification (editing or transgenesis).Fil: Goytia Bertero, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas Químicas y Naturales. Cátedra de Ingeniería Genética; ArgentinaFil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Arteaga, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Auteri, Micol Tais. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Ingeniería Genética; ArgentinaSpringer2020-10-28T22:16:35Z2020-10-28T22:16:35Z2020-07-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8145https://link.springer.com/article/10.1007%2Fs11627-020-10098-51054-5476https://doi.org/10.1007/s11627-020-10098-5In Vitro Cellular & Developmental Biology - Plant (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:03Zoai:localhost:20.500.12123/8145instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:03.501INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
title High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
spellingShingle High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
Goytia Bertero, Valentina
Minthostachys
Somatic Embryogenesis
In Vitro Culture
Embriogénesis Somática
Cultivo in Vitro
Griseb
Peperina
title_short High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
title_full High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
title_fullStr High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
title_full_unstemmed High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
title_sort High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
dc.creator.none.fl_str_mv Goytia Bertero, Valentina
Beznec, Ailin
Faccio, Paula Daniela
Auteri, Micol Tais
Arteaga, Martin
Bonafede, Marcos
Bossio, Maria Emilia
author Goytia Bertero, Valentina
author_facet Goytia Bertero, Valentina
Beznec, Ailin
Faccio, Paula Daniela
Auteri, Micol Tais
Arteaga, Martin
Bonafede, Marcos
Bossio, Maria Emilia
author_role author
author2 Beznec, Ailin
Faccio, Paula Daniela
Auteri, Micol Tais
Arteaga, Martin
Bonafede, Marcos
Bossio, Maria Emilia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Minthostachys
Somatic Embryogenesis
In Vitro Culture
Embriogénesis Somática
Cultivo in Vitro
Griseb
Peperina
topic Minthostachys
Somatic Embryogenesis
In Vitro Culture
Embriogénesis Somática
Cultivo in Vitro
Griseb
Peperina
dc.description.none.fl_txt_mv In vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for “peperina” plants (Minthostachys verticillata (Griseb.) Epling). In vitro shoots developed via shoot apex extracted from greenhouse-grown plants were cultured on shoot elongation medium (ShM) consisting of Murashige and Skoog (MS) basal salts supplemented with myoinositol, thiamine, and benzyladenine. Shoot apexes were disinfected with 70% ethanol for 5 min and 0.26 sodium hypochlorite (w/v) for 5 min, before the initiation of in vitro culture. For somatic embryo (SE) induction, leaves collected from 2-mo-old in vitro raised shoots were cultured on MS basal medium supplemented with benzyladenine and two different concentrations of coconut water (CW) until SE developed. Using 2.5% CW-supplemented medium, 100% of cultured leaves developed SE and 89.3% of the leaf explants developed plantlets. The resulting embryos germinated on the same medium, and the plantlets obtained were transferred onto ShM until they developed 3 to 4 leaves. To increase the low root development, shoots were transferred into fully hydrated perlite for rooting and then transplanted into soil-perlite containing pots for hardening. This protocol provides the first step to supply the demand and simultaneously protect the natural populations of Minthostachys verticillata from overexploitation. Furthermore, this protocol provides the first step for crop improvement by genetic modification (editing or transgenesis).
Fil: Goytia Bertero, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas Químicas y Naturales. Cátedra de Ingeniería Genética; Argentina
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Arteaga, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Auteri, Micol Tais. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Ingeniería Genética; Argentina
description In vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for “peperina” plants (Minthostachys verticillata (Griseb.) Epling). In vitro shoots developed via shoot apex extracted from greenhouse-grown plants were cultured on shoot elongation medium (ShM) consisting of Murashige and Skoog (MS) basal salts supplemented with myoinositol, thiamine, and benzyladenine. Shoot apexes were disinfected with 70% ethanol for 5 min and 0.26 sodium hypochlorite (w/v) for 5 min, before the initiation of in vitro culture. For somatic embryo (SE) induction, leaves collected from 2-mo-old in vitro raised shoots were cultured on MS basal medium supplemented with benzyladenine and two different concentrations of coconut water (CW) until SE developed. Using 2.5% CW-supplemented medium, 100% of cultured leaves developed SE and 89.3% of the leaf explants developed plantlets. The resulting embryos germinated on the same medium, and the plantlets obtained were transferred onto ShM until they developed 3 to 4 leaves. To increase the low root development, shoots were transferred into fully hydrated perlite for rooting and then transplanted into soil-perlite containing pots for hardening. This protocol provides the first step to supply the demand and simultaneously protect the natural populations of Minthostachys verticillata from overexploitation. Furthermore, this protocol provides the first step for crop improvement by genetic modification (editing or transgenesis).
publishDate 2020
dc.date.none.fl_str_mv 2020-10-28T22:16:35Z
2020-10-28T22:16:35Z
2020-07-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8145
https://link.springer.com/article/10.1007%2Fs11627-020-10098-5
1054-5476
https://doi.org/10.1007/s11627-020-10098-5
url http://hdl.handle.net/20.500.12123/8145
https://link.springer.com/article/10.1007%2Fs11627-020-10098-5
https://doi.org/10.1007/s11627-020-10098-5
identifier_str_mv 1054-5476
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv In Vitro Cellular & Developmental Biology - Plant (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.558318

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