Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes

Autores
Garcia, José María; Andrada, Aldo R.; Erazzu, Luis Ernesto; Acevedo, Alberto
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Modern sugarcane (Saccharum spp.) cultivars originated from an intricate hybridization process, resulting in complex polyploid and aneuploid genomes. Flow cytometry (FCM), which has emerged as a precise and fast method for research on genome size in plants, has potential to unveil key aspects of sugarcane cytogenetics. In this study, FCM probes were conducted to estimate 2C DNA content in 175 Saccharum genotypes embracing phenotypically contrasting canes (commercial cultivars, energy-cane hybrids, and a Saccharum spontaneum accession) and full-sib sugarcane families. FCM-mediated DNA content estimates exhibited high accuracy in predicting chromosome numbers, offering a time-efficient alternative to classical cytogenetic procedure. Low-magnitude correlations detected between DNA content and phenotypic traits suggest that indirect selection of agronomic traits using FCM-mediated DNA content estimates may not be efficient. DNA content variation patterns in sugarcane families varied according to the crossing, and revealed transgressive variations associated with rampant aneuploidy. Altogether, the findings uncover that loss or gain of genetic material constitutes a source of high genetic variability that may substantially impact on crop breeding.
EEA Famaillá
Fil: García, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Andrada, Aldo R. Fundación M. Lillo. Instituto de Genética y Microbiología. Area Biología Integrativa; Argentina
Fil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fuente
Plant Breeding : 1-13. (First published: 29 July 2024)
Materia
Caña de Azúcar
Variación Genética
Genotipos
Citometría de Flujo (Células)
Poliploidia
Variación Fenotípica
Sugar Cane
Genetic Variation
Genotypes
Flow Cytometry
Polyploidy
Phenotypic Variation
Saccharum
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypesGarcia, José MaríaAndrada, Aldo R.Erazzu, Luis ErnestoAcevedo, AlbertoCaña de AzúcarVariación GenéticaGenotiposCitometría de Flujo (Células)PoliploidiaVariación FenotípicaSugar CaneGenetic VariationGenotypesFlow CytometryPolyploidyPhenotypic VariationSaccharumModern sugarcane (Saccharum spp.) cultivars originated from an intricate hybridization process, resulting in complex polyploid and aneuploid genomes. Flow cytometry (FCM), which has emerged as a precise and fast method for research on genome size in plants, has potential to unveil key aspects of sugarcane cytogenetics. In this study, FCM probes were conducted to estimate 2C DNA content in 175 Saccharum genotypes embracing phenotypically contrasting canes (commercial cultivars, energy-cane hybrids, and a Saccharum spontaneum accession) and full-sib sugarcane families. FCM-mediated DNA content estimates exhibited high accuracy in predicting chromosome numbers, offering a time-efficient alternative to classical cytogenetic procedure. Low-magnitude correlations detected between DNA content and phenotypic traits suggest that indirect selection of agronomic traits using FCM-mediated DNA content estimates may not be efficient. DNA content variation patterns in sugarcane families varied according to the crossing, and revealed transgressive variations associated with rampant aneuploidy. Altogether, the findings uncover that loss or gain of genetic material constitutes a source of high genetic variability that may substantially impact on crop breeding.EEA FamailláFil: García, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Andrada, Aldo R. Fundación M. Lillo. Instituto de Genética y Microbiología. Area Biología Integrativa; ArgentinaFil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.Fil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; ArgentinaWiley2024-08-02T13:13:51Z2024-08-02T13:13:51Z2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18773https://onlinelibrary.wiley.com/doi/10.1111/pbr.132030179-95411439-0523https://doi.org/10.1111/pbr.13203Plant Breeding : 1-13. (First published: 29 July 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climáticoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:41Zoai:localhost:20.500.12123/18773instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:41.96INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
title Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
spellingShingle Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
Garcia, José María
Caña de Azúcar
Variación Genética
Genotipos
Citometría de Flujo (Células)
Poliploidia
Variación Fenotípica
Sugar Cane
Genetic Variation
Genotypes
Flow Cytometry
Polyploidy
Phenotypic Variation
Saccharum
title_short Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
title_full Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
title_fullStr Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
title_full_unstemmed Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
title_sort Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
dc.creator.none.fl_str_mv Garcia, José María
Andrada, Aldo R.
Erazzu, Luis Ernesto
Acevedo, Alberto
author Garcia, José María
author_facet Garcia, José María
Andrada, Aldo R.
Erazzu, Luis Ernesto
Acevedo, Alberto
author_role author
author2 Andrada, Aldo R.
Erazzu, Luis Ernesto
Acevedo, Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Caña de Azúcar
Variación Genética
Genotipos
Citometría de Flujo (Células)
Poliploidia
Variación Fenotípica
Sugar Cane
Genetic Variation
Genotypes
Flow Cytometry
Polyploidy
Phenotypic Variation
Saccharum
topic Caña de Azúcar
Variación Genética
Genotipos
Citometría de Flujo (Células)
Poliploidia
Variación Fenotípica
Sugar Cane
Genetic Variation
Genotypes
Flow Cytometry
Polyploidy
Phenotypic Variation
Saccharum
dc.description.none.fl_txt_mv Modern sugarcane (Saccharum spp.) cultivars originated from an intricate hybridization process, resulting in complex polyploid and aneuploid genomes. Flow cytometry (FCM), which has emerged as a precise and fast method for research on genome size in plants, has potential to unveil key aspects of sugarcane cytogenetics. In this study, FCM probes were conducted to estimate 2C DNA content in 175 Saccharum genotypes embracing phenotypically contrasting canes (commercial cultivars, energy-cane hybrids, and a Saccharum spontaneum accession) and full-sib sugarcane families. FCM-mediated DNA content estimates exhibited high accuracy in predicting chromosome numbers, offering a time-efficient alternative to classical cytogenetic procedure. Low-magnitude correlations detected between DNA content and phenotypic traits suggest that indirect selection of agronomic traits using FCM-mediated DNA content estimates may not be efficient. DNA content variation patterns in sugarcane families varied according to the crossing, and revealed transgressive variations associated with rampant aneuploidy. Altogether, the findings uncover that loss or gain of genetic material constitutes a source of high genetic variability that may substantially impact on crop breeding.
EEA Famaillá
Fil: García, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Andrada, Aldo R. Fundación M. Lillo. Instituto de Genética y Microbiología. Area Biología Integrativa; Argentina
Fil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
description Modern sugarcane (Saccharum spp.) cultivars originated from an intricate hybridization process, resulting in complex polyploid and aneuploid genomes. Flow cytometry (FCM), which has emerged as a precise and fast method for research on genome size in plants, has potential to unveil key aspects of sugarcane cytogenetics. In this study, FCM probes were conducted to estimate 2C DNA content in 175 Saccharum genotypes embracing phenotypically contrasting canes (commercial cultivars, energy-cane hybrids, and a Saccharum spontaneum accession) and full-sib sugarcane families. FCM-mediated DNA content estimates exhibited high accuracy in predicting chromosome numbers, offering a time-efficient alternative to classical cytogenetic procedure. Low-magnitude correlations detected between DNA content and phenotypic traits suggest that indirect selection of agronomic traits using FCM-mediated DNA content estimates may not be efficient. DNA content variation patterns in sugarcane families varied according to the crossing, and revealed transgressive variations associated with rampant aneuploidy. Altogether, the findings uncover that loss or gain of genetic material constitutes a source of high genetic variability that may substantially impact on crop breeding.
publishDate 2024
dc.date.none.fl_str_mv 2024-08-02T13:13:51Z
2024-08-02T13:13:51Z
2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18773
https://onlinelibrary.wiley.com/doi/10.1111/pbr.13203
0179-9541
1439-0523
https://doi.org/10.1111/pbr.13203
url http://hdl.handle.net/20.500.12123/18773
https://onlinelibrary.wiley.com/doi/10.1111/pbr.13203
https://doi.org/10.1111/pbr.13203
identifier_str_mv 0179-9541
1439-0523
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climático
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant Breeding : 1-13. (First published: 29 July 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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