Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype

Autores
Di Pauli, Valentina; Fontana, Paola Daniela; Lewi, Dalia Marcela; Felipe, Arturo; Erazzu, Luis Ernesto
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The complexity of the sugarcane genome, the narrow genetic base of subtropical germplasm, and the extended breeding and selection cycle pose significant challenges to conventional breeding methods. In vitro mutagenesis offers an alternative approach to generating genetic variability by harnessing both somaclonal variation and induced mutations. An in vitro mutagenesis protocol, using the chemical mutagen ethyl methanesulfonate (EMS) in an elite Argentine sugarcane cultivar, was developed. The resulting genetic variability was assessed in the field in plant and first ratoon crops. Six-week-old embryogenic calli were treated with 8, 16, 32, and 48 mM EMS. Plants were regenerated from treated calli and acclimatized in a greenhouse. The lethal dose LD50 for regeneration capacity was 31.68 mM EMS, whereas LD25 was 17.71 mM EMS. Based on these results, the plants from the 16 and 32 mM EMS treatments were phenotypically assessed in the field. These assessments showed that EMS treatments exhibited phenotypic variability due to genetic changes. Traits such as the number of stalks, stalk length, stalk weight, internode length, and Brix showed an increase in the population mean in the EMS treatments compared with the wild type. Some traits maintained changes from one year to the next, whereas others reverted to the wild-type genotype values in the second year. Mutagenic treatments generated greater and more stable genetic variation than somaclonal variation, supporting their use as effective tools for sugarcane improvement. Based on agronomic traits, 23 mutant plants were selected and advanced to subsequent generations.
EEA Famaillá
Fil: Di Pauli, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Felipe, Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fuente
Discover Plants 2 : article number 230. (July 2025)
Materia
Caña de Azúcar
Genotipos
Variación Genética
Ems
Argentina
Sugar Cane
Genotypes
Genetic Variation
Mutagenesis
EMS
Etilmetanosulfonato
Ethylmethanesulphonate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23188
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oai_identifier_str oai:localhost:20.500.12123/23188
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network_name_str INTA Digital (INTA)
spelling Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotypeDi Pauli, ValentinaFontana, Paola DanielaLewi, Dalia MarcelaFelipe, ArturoErazzu, Luis ErnestoCaña de AzúcarGenotiposVariación GenéticaEmsArgentinaSugar CaneGenotypesGenetic VariationMutagenesisEMSEtilmetanosulfonatoEthylmethanesulphonateThe complexity of the sugarcane genome, the narrow genetic base of subtropical germplasm, and the extended breeding and selection cycle pose significant challenges to conventional breeding methods. In vitro mutagenesis offers an alternative approach to generating genetic variability by harnessing both somaclonal variation and induced mutations. An in vitro mutagenesis protocol, using the chemical mutagen ethyl methanesulfonate (EMS) in an elite Argentine sugarcane cultivar, was developed. The resulting genetic variability was assessed in the field in plant and first ratoon crops. Six-week-old embryogenic calli were treated with 8, 16, 32, and 48 mM EMS. Plants were regenerated from treated calli and acclimatized in a greenhouse. The lethal dose LD50 for regeneration capacity was 31.68 mM EMS, whereas LD25 was 17.71 mM EMS. Based on these results, the plants from the 16 and 32 mM EMS treatments were phenotypically assessed in the field. These assessments showed that EMS treatments exhibited phenotypic variability due to genetic changes. Traits such as the number of stalks, stalk length, stalk weight, internode length, and Brix showed an increase in the population mean in the EMS treatments compared with the wild type. Some traits maintained changes from one year to the next, whereas others reverted to the wild-type genotype values in the second year. Mutagenic treatments generated greater and more stable genetic variation than somaclonal variation, supporting their use as effective tools for sugarcane improvement. Based on agronomic traits, 23 mutant plants were selected and advanced to subsequent generations.EEA FamailláFil: Di Pauli, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Felipe, Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.Springer Nature2025-07-28T16:09:29Z2025-07-28T16:09:29Z2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23188https://link.springer.com/article/10.1007/s44372-025-00298-x3005-1207https://doi.org/10.1007/s44372-025-00298-xDiscover Plants 2 : article number 230. (July 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climáticoinfo:eu-repograntAgreement/INTA/2023-PD-L01-I086, Edición génica, transgénesis y mutaciones inducidas como generadores de nueva variabilidad en especies de interés agropecuarioinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:27Zoai:localhost:20.500.12123/23188instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:27.752INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
title Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
spellingShingle Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
Di Pauli, Valentina
Caña de Azúcar
Genotipos
Variación Genética
Ems
Argentina
Sugar Cane
Genotypes
Genetic Variation
Mutagenesis
EMS
Etilmetanosulfonato
Ethylmethanesulphonate
title_short Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
title_full Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
title_fullStr Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
title_full_unstemmed Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
title_sort Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
dc.creator.none.fl_str_mv Di Pauli, Valentina
Fontana, Paola Daniela
Lewi, Dalia Marcela
Felipe, Arturo
Erazzu, Luis Ernesto
author Di Pauli, Valentina
author_facet Di Pauli, Valentina
Fontana, Paola Daniela
Lewi, Dalia Marcela
Felipe, Arturo
Erazzu, Luis Ernesto
author_role author
author2 Fontana, Paola Daniela
Lewi, Dalia Marcela
Felipe, Arturo
Erazzu, Luis Ernesto
author2_role author
author
author
author
dc.subject.none.fl_str_mv Caña de Azúcar
Genotipos
Variación Genética
Ems
Argentina
Sugar Cane
Genotypes
Genetic Variation
Mutagenesis
EMS
Etilmetanosulfonato
Ethylmethanesulphonate
topic Caña de Azúcar
Genotipos
Variación Genética
Ems
Argentina
Sugar Cane
Genotypes
Genetic Variation
Mutagenesis
EMS
Etilmetanosulfonato
Ethylmethanesulphonate
dc.description.none.fl_txt_mv The complexity of the sugarcane genome, the narrow genetic base of subtropical germplasm, and the extended breeding and selection cycle pose significant challenges to conventional breeding methods. In vitro mutagenesis offers an alternative approach to generating genetic variability by harnessing both somaclonal variation and induced mutations. An in vitro mutagenesis protocol, using the chemical mutagen ethyl methanesulfonate (EMS) in an elite Argentine sugarcane cultivar, was developed. The resulting genetic variability was assessed in the field in plant and first ratoon crops. Six-week-old embryogenic calli were treated with 8, 16, 32, and 48 mM EMS. Plants were regenerated from treated calli and acclimatized in a greenhouse. The lethal dose LD50 for regeneration capacity was 31.68 mM EMS, whereas LD25 was 17.71 mM EMS. Based on these results, the plants from the 16 and 32 mM EMS treatments were phenotypically assessed in the field. These assessments showed that EMS treatments exhibited phenotypic variability due to genetic changes. Traits such as the number of stalks, stalk length, stalk weight, internode length, and Brix showed an increase in the population mean in the EMS treatments compared with the wild type. Some traits maintained changes from one year to the next, whereas others reverted to the wild-type genotype values in the second year. Mutagenic treatments generated greater and more stable genetic variation than somaclonal variation, supporting their use as effective tools for sugarcane improvement. Based on agronomic traits, 23 mutant plants were selected and advanced to subsequent generations.
EEA Famaillá
Fil: Di Pauli, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Felipe, Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
description The complexity of the sugarcane genome, the narrow genetic base of subtropical germplasm, and the extended breeding and selection cycle pose significant challenges to conventional breeding methods. In vitro mutagenesis offers an alternative approach to generating genetic variability by harnessing both somaclonal variation and induced mutations. An in vitro mutagenesis protocol, using the chemical mutagen ethyl methanesulfonate (EMS) in an elite Argentine sugarcane cultivar, was developed. The resulting genetic variability was assessed in the field in plant and first ratoon crops. Six-week-old embryogenic calli were treated with 8, 16, 32, and 48 mM EMS. Plants were regenerated from treated calli and acclimatized in a greenhouse. The lethal dose LD50 for regeneration capacity was 31.68 mM EMS, whereas LD25 was 17.71 mM EMS. Based on these results, the plants from the 16 and 32 mM EMS treatments were phenotypically assessed in the field. These assessments showed that EMS treatments exhibited phenotypic variability due to genetic changes. Traits such as the number of stalks, stalk length, stalk weight, internode length, and Brix showed an increase in the population mean in the EMS treatments compared with the wild type. Some traits maintained changes from one year to the next, whereas others reverted to the wild-type genotype values in the second year. Mutagenic treatments generated greater and more stable genetic variation than somaclonal variation, supporting their use as effective tools for sugarcane improvement. Based on agronomic traits, 23 mutant plants were selected and advanced to subsequent generations.
publishDate 2025
dc.date.none.fl_str_mv 2025-07-28T16:09:29Z
2025-07-28T16:09:29Z
2025-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23188
https://link.springer.com/article/10.1007/s44372-025-00298-x
3005-1207
https://doi.org/10.1007/s44372-025-00298-x
url http://hdl.handle.net/20.500.12123/23188
https://link.springer.com/article/10.1007/s44372-025-00298-x
https://doi.org/10.1007/s44372-025-00298-x
identifier_str_mv 3005-1207
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climático
info:eu-repograntAgreement/INTA/2023-PD-L01-I086, Edición génica, transgénesis y mutaciones inducidas como generadores de nueva variabilidad en especies de interés agropecuario
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Discover Plants 2 : article number 230. (July 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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