Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
- Autores
- Pearce, Stephen; Tabbita, Facundo; Cantu, Dario; Buffalo, Vince; Avni, Raz; Vazquez Gross, Hans; Zhao, Rongrong; Conley, Christopher J.; Distelfeld, Assaf; Dubcovsky, Jorge
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background; During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. Results: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. Conclusions: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.
Instituto de Recursos Biológicos
Fil: Pearce, Stephen. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Cantu, Dario. University of California. Department of Viticulture and Enology; Estados Unidos
Fil: Buffalo, Vince. University of California. Department of Plant Sciences; Estados Unidos
Fil: Avni, Raz. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; Israel
Fil: Vazquez Gross, Hans. University of California. Department of Plant Sciences; Estados Unidos
Fil: Zhao, Rongrong. China Agricultural University. College of Resources and Environmental Science. Department of Plant Nutrition; China
Fil: Conley, Christopher J. University of California. Department of Statistics; Estados Unidos
Fil: Distelfeld, Assaf. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; Israel
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos - Fuente
- BMC Plant Biology 14 : 368 (2014)
- Materia
-
Trigo
Avejentamiento
Genes
Genética
Cinc
Hierro
Wheat
Senescence
Genetics
Zinc
Iron
Zn
Fe - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/3369
Ver los metadatos del registro completo
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Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescencePearce, StephenTabbita, FacundoCantu, DarioBuffalo, VinceAvni, RazVazquez Gross, HansZhao, RongrongConley, Christopher J.Distelfeld, AssafDubcovsky, JorgeTrigoAvejentamientoGenesGenéticaCincHierroWheatSenescenceGeneticsZincIronZnFeBackground; During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. Results: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. Conclusions: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.Instituto de Recursos BiológicosFil: Pearce, Stephen. University of California. Department of Plant Sciences; Estados UnidosFil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Cantu, Dario. University of California. Department of Viticulture and Enology; Estados UnidosFil: Buffalo, Vince. University of California. Department of Plant Sciences; Estados UnidosFil: Avni, Raz. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; IsraelFil: Vazquez Gross, Hans. University of California. Department of Plant Sciences; Estados UnidosFil: Zhao, Rongrong. China Agricultural University. College of Resources and Environmental Science. Department of Plant Nutrition; ChinaFil: Conley, Christopher J. University of California. Department of Statistics; Estados UnidosFil: Distelfeld, Assaf. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; IsraelFil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos2018-09-17T12:37:39Z2018-09-17T12:37:39Z2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0368-2http://hdl.handle.net/20.500.12123/33691471-2229https://doi.org/10.1186/s12870-014-0368-2BMC Plant Biology 14 : 368 (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:26Zoai:localhost:20.500.12123/3369instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:26.738INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence |
title |
Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence |
spellingShingle |
Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence Pearce, Stephen Trigo Avejentamiento Genes Genética Cinc Hierro Wheat Senescence Genetics Zinc Iron Zn Fe |
title_short |
Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence |
title_full |
Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence |
title_fullStr |
Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence |
title_full_unstemmed |
Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence |
title_sort |
Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence |
dc.creator.none.fl_str_mv |
Pearce, Stephen Tabbita, Facundo Cantu, Dario Buffalo, Vince Avni, Raz Vazquez Gross, Hans Zhao, Rongrong Conley, Christopher J. Distelfeld, Assaf Dubcovsky, Jorge |
author |
Pearce, Stephen |
author_facet |
Pearce, Stephen Tabbita, Facundo Cantu, Dario Buffalo, Vince Avni, Raz Vazquez Gross, Hans Zhao, Rongrong Conley, Christopher J. Distelfeld, Assaf Dubcovsky, Jorge |
author_role |
author |
author2 |
Tabbita, Facundo Cantu, Dario Buffalo, Vince Avni, Raz Vazquez Gross, Hans Zhao, Rongrong Conley, Christopher J. Distelfeld, Assaf Dubcovsky, Jorge |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Trigo Avejentamiento Genes Genética Cinc Hierro Wheat Senescence Genetics Zinc Iron Zn Fe |
topic |
Trigo Avejentamiento Genes Genética Cinc Hierro Wheat Senescence Genetics Zinc Iron Zn Fe |
dc.description.none.fl_txt_mv |
Background; During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. Results: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. Conclusions: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world. Instituto de Recursos Biológicos Fil: Pearce, Stephen. University of California. Department of Plant Sciences; Estados Unidos Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Cantu, Dario. University of California. Department of Viticulture and Enology; Estados Unidos Fil: Buffalo, Vince. University of California. Department of Plant Sciences; Estados Unidos Fil: Avni, Raz. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; Israel Fil: Vazquez Gross, Hans. University of California. Department of Plant Sciences; Estados Unidos Fil: Zhao, Rongrong. China Agricultural University. College of Resources and Environmental Science. Department of Plant Nutrition; China Fil: Conley, Christopher J. University of California. Department of Statistics; Estados Unidos Fil: Distelfeld, Assaf. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; Israel Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos |
description |
Background; During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. Results: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. Conclusions: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12 2018-09-17T12:37:39Z 2018-09-17T12:37:39Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0368-2 http://hdl.handle.net/20.500.12123/3369 1471-2229 https://doi.org/10.1186/s12870-014-0368-2 |
url |
https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0368-2 http://hdl.handle.net/20.500.12123/3369 https://doi.org/10.1186/s12870-014-0368-2 |
identifier_str_mv |
1471-2229 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
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