Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence

Autores
Pearce, Stephen; Tabbita, Facundo; Cantu, Dario; Buffalo, Vince; Avni, Raz; Vazquez Gross, Hans; Zhao, Rongrong; Conley, Christopher J.; Distelfeld, Assaf; Dubcovsky, Jorge
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background; During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. Results: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. Conclusions: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.
Instituto de Recursos Biológicos
Fil: Pearce, Stephen. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Cantu, Dario. University of California. Department of Viticulture and Enology; Estados Unidos
Fil: Buffalo, Vince. University of California. Department of Plant Sciences; Estados Unidos
Fil: Avni, Raz. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; Israel
Fil: Vazquez Gross, Hans. University of California. Department of Plant Sciences; Estados Unidos
Fil: Zhao, Rongrong. China Agricultural University. College of Resources and Environmental Science. Department of Plant Nutrition; China
Fil: Conley, Christopher J. University of California. Department of Statistics; Estados Unidos
Fil: Distelfeld, Assaf. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; Israel
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Fuente
BMC Plant Biology 14 : 368 (2014)
Materia
Trigo
Avejentamiento
Genes
Genética
Cinc
Hierro
Wheat
Senescence
Genetics
Zinc
Iron
Zn
Fe
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/3369
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network_name_str INTA Digital (INTA)
spelling Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescencePearce, StephenTabbita, FacundoCantu, DarioBuffalo, VinceAvni, RazVazquez Gross, HansZhao, RongrongConley, Christopher J.Distelfeld, AssafDubcovsky, JorgeTrigoAvejentamientoGenesGenéticaCincHierroWheatSenescenceGeneticsZincIronZnFeBackground; During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. Results: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. Conclusions: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.Instituto de Recursos BiológicosFil: Pearce, Stephen. University of California. Department of Plant Sciences; Estados UnidosFil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Cantu, Dario. University of California. Department of Viticulture and Enology; Estados UnidosFil: Buffalo, Vince. University of California. Department of Plant Sciences; Estados UnidosFil: Avni, Raz. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; IsraelFil: Vazquez Gross, Hans. University of California. Department of Plant Sciences; Estados UnidosFil: Zhao, Rongrong. China Agricultural University. College of Resources and Environmental Science. Department of Plant Nutrition; ChinaFil: Conley, Christopher J. University of California. Department of Statistics; Estados UnidosFil: Distelfeld, Assaf. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; IsraelFil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos2018-09-17T12:37:39Z2018-09-17T12:37:39Z2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0368-2http://hdl.handle.net/20.500.12123/33691471-2229https://doi.org/10.1186/s12870-014-0368-2BMC Plant Biology 14 : 368 (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:26Zoai:localhost:20.500.12123/3369instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:26.738INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
title Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
spellingShingle Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
Pearce, Stephen
Trigo
Avejentamiento
Genes
Genética
Cinc
Hierro
Wheat
Senescence
Genetics
Zinc
Iron
Zn
Fe
title_short Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
title_full Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
title_fullStr Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
title_full_unstemmed Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
title_sort Regulation of Zn and Fe transporters by the GPC1gene during early wheat monocarpic senescence
dc.creator.none.fl_str_mv Pearce, Stephen
Tabbita, Facundo
Cantu, Dario
Buffalo, Vince
Avni, Raz
Vazquez Gross, Hans
Zhao, Rongrong
Conley, Christopher J.
Distelfeld, Assaf
Dubcovsky, Jorge
author Pearce, Stephen
author_facet Pearce, Stephen
Tabbita, Facundo
Cantu, Dario
Buffalo, Vince
Avni, Raz
Vazquez Gross, Hans
Zhao, Rongrong
Conley, Christopher J.
Distelfeld, Assaf
Dubcovsky, Jorge
author_role author
author2 Tabbita, Facundo
Cantu, Dario
Buffalo, Vince
Avni, Raz
Vazquez Gross, Hans
Zhao, Rongrong
Conley, Christopher J.
Distelfeld, Assaf
Dubcovsky, Jorge
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trigo
Avejentamiento
Genes
Genética
Cinc
Hierro
Wheat
Senescence
Genetics
Zinc
Iron
Zn
Fe
topic Trigo
Avejentamiento
Genes
Genética
Cinc
Hierro
Wheat
Senescence
Genetics
Zinc
Iron
Zn
Fe
dc.description.none.fl_txt_mv Background; During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. Results: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. Conclusions: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.
Instituto de Recursos Biológicos
Fil: Pearce, Stephen. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Cantu, Dario. University of California. Department of Viticulture and Enology; Estados Unidos
Fil: Buffalo, Vince. University of California. Department of Plant Sciences; Estados Unidos
Fil: Avni, Raz. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; Israel
Fil: Vazquez Gross, Hans. University of California. Department of Plant Sciences; Estados Unidos
Fil: Zhao, Rongrong. China Agricultural University. College of Resources and Environmental Science. Department of Plant Nutrition; China
Fil: Conley, Christopher J. University of California. Department of Statistics; Estados Unidos
Fil: Distelfeld, Assaf. Tel Aviv University. Faculty of Life Sciences. Department of Molecular Biology and Ecology of Plants; Israel
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
description Background; During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. Results: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. Conclusions: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
2018-09-17T12:37:39Z
2018-09-17T12:37:39Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0368-2
http://hdl.handle.net/20.500.12123/3369
1471-2229
https://doi.org/10.1186/s12870-014-0368-2
url https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0368-2
http://hdl.handle.net/20.500.12123/3369
https://doi.org/10.1186/s12870-014-0368-2
identifier_str_mv 1471-2229
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv BMC Plant Biology 14 : 368 (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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