Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence

Autores
Pearce, Stephen; Tabbita, Facundo; Cantu, Dario; Buffalo, Vince; Avni, Raz; Vazquez Gross, Hans; Zhao, Rongrong; Conley, Christopher J.; Distelfeld, Assaf; Dubcovsky, Jorge
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. RESULTS: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. CONCLUSIONS: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.
Fil: Pearce, Stephen. University of California; Estados Unidos
Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Cantu, Dario. University of California; Estados Unidos
Fil: Buffalo, Vince. University of California; Estados Unidos
Fil: Avni, Raz. Tel Aviv University; Israel
Fil: Vazquez Gross, Hans. University of California; Estados Unidos
Fil: Zhao, Rongrong. China Agricultural University; China
Fil: Conley, Christopher J.. University of California; Estados Unidos
Fil: Distelfeld, Assaf. Faculty Of Life Sciences, Department Of Molecular Biolo;
Fil: Dubcovsky, Jorge. University of California; Estados Unidos. Howard Hughes Medical Institute ; Estados Unidos. Gordon & Betty Moore Foundation Investigator; Estados Unidos
Materia
Wheat
Senescence
Zinc transport
Iron transport
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32851

id CONICETDig_a1e74e054545604281d19f48fb22836b
oai_identifier_str oai:ri.conicet.gov.ar:11336/32851
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescencePearce, StephenTabbita, FacundoCantu, DarioBuffalo, VinceAvni, RazVazquez Gross, HansZhao, RongrongConley, Christopher J.Distelfeld, AssafDubcovsky, JorgeWheatSenescenceZinc transportIron transporthttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4BACKGROUND: During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. RESULTS: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. CONCLUSIONS: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.Fil: Pearce, Stephen. University of California; Estados UnidosFil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaFil: Cantu, Dario. University of California; Estados UnidosFil: Buffalo, Vince. University of California; Estados UnidosFil: Avni, Raz. Tel Aviv University; IsraelFil: Vazquez Gross, Hans. University of California; Estados UnidosFil: Zhao, Rongrong. China Agricultural University; ChinaFil: Conley, Christopher J.. University of California; Estados UnidosFil: Distelfeld, Assaf. Faculty Of Life Sciences, Department Of Molecular Biolo;Fil: Dubcovsky, Jorge. University of California; Estados Unidos. Howard Hughes Medical Institute ; Estados Unidos. Gordon & Betty Moore Foundation Investigator; Estados UnidosBioMed Central2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32851Cantu, Dario; Tabbita, Facundo; Dubcovsky, Jorge; Zhao, Rongrong; Vazquez Gross, Hans; Distelfeld, Assaf; et al.; Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence; BioMed Central; BMC Plant Biology; 14; 1; 2-2014; 368-2911471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/content/pdf/s12870-014-0368-2.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-014-0368-2info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302714/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:26Zoai:ri.conicet.gov.ar:11336/32851instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:26.871CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
title Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
spellingShingle Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
Pearce, Stephen
Wheat
Senescence
Zinc transport
Iron transport
title_short Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
title_full Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
title_fullStr Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
title_full_unstemmed Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
title_sort Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
dc.creator.none.fl_str_mv Pearce, Stephen
Tabbita, Facundo
Cantu, Dario
Buffalo, Vince
Avni, Raz
Vazquez Gross, Hans
Zhao, Rongrong
Conley, Christopher J.
Distelfeld, Assaf
Dubcovsky, Jorge
author Pearce, Stephen
author_facet Pearce, Stephen
Tabbita, Facundo
Cantu, Dario
Buffalo, Vince
Avni, Raz
Vazquez Gross, Hans
Zhao, Rongrong
Conley, Christopher J.
Distelfeld, Assaf
Dubcovsky, Jorge
author_role author
author2 Tabbita, Facundo
Cantu, Dario
Buffalo, Vince
Avni, Raz
Vazquez Gross, Hans
Zhao, Rongrong
Conley, Christopher J.
Distelfeld, Assaf
Dubcovsky, Jorge
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Wheat
Senescence
Zinc transport
Iron transport
topic Wheat
Senescence
Zinc transport
Iron transport
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv BACKGROUND: During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. RESULTS: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. CONCLUSIONS: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.
Fil: Pearce, Stephen. University of California; Estados Unidos
Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Cantu, Dario. University of California; Estados Unidos
Fil: Buffalo, Vince. University of California; Estados Unidos
Fil: Avni, Raz. Tel Aviv University; Israel
Fil: Vazquez Gross, Hans. University of California; Estados Unidos
Fil: Zhao, Rongrong. China Agricultural University; China
Fil: Conley, Christopher J.. University of California; Estados Unidos
Fil: Distelfeld, Assaf. Faculty Of Life Sciences, Department Of Molecular Biolo;
Fil: Dubcovsky, Jorge. University of California; Estados Unidos. Howard Hughes Medical Institute ; Estados Unidos. Gordon & Betty Moore Foundation Investigator; Estados Unidos
description BACKGROUND: During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. RESULTS: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. CONCLUSIONS: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32851
Cantu, Dario; Tabbita, Facundo; Dubcovsky, Jorge; Zhao, Rongrong; Vazquez Gross, Hans; Distelfeld, Assaf; et al.; Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence; BioMed Central; BMC Plant Biology; 14; 1; 2-2014; 368-291
1471-2229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32851
identifier_str_mv Cantu, Dario; Tabbita, Facundo; Dubcovsky, Jorge; Zhao, Rongrong; Vazquez Gross, Hans; Distelfeld, Assaf; et al.; Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence; BioMed Central; BMC Plant Biology; 14; 1; 2-2014; 368-291
1471-2229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/content/pdf/s12870-014-0368-2.pdf
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-014-0368-2
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302714/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613870179581952
score 13.070432