Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence
- Autores
- Pearce, Stephen; Tabbita, Facundo; Cantu, Dario; Buffalo, Vince; Avni, Raz; Vazquez Gross, Hans; Zhao, Rongrong; Conley, Christopher J.; Distelfeld, Assaf; Dubcovsky, Jorge
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- BACKGROUND: During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. RESULTS: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. CONCLUSIONS: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.
Fil: Pearce, Stephen. University of California; Estados Unidos
Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Cantu, Dario. University of California; Estados Unidos
Fil: Buffalo, Vince. University of California; Estados Unidos
Fil: Avni, Raz. Tel Aviv University; Israel
Fil: Vazquez Gross, Hans. University of California; Estados Unidos
Fil: Zhao, Rongrong. China Agricultural University; China
Fil: Conley, Christopher J.. University of California; Estados Unidos
Fil: Distelfeld, Assaf. Faculty Of Life Sciences, Department Of Molecular Biolo;
Fil: Dubcovsky, Jorge. University of California; Estados Unidos. Howard Hughes Medical Institute ; Estados Unidos. Gordon & Betty Moore Foundation Investigator; Estados Unidos - Materia
-
Wheat
Senescence
Zinc transport
Iron transport - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/32851
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3498 |
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CONICET Digital (CONICET) |
spelling |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescencePearce, StephenTabbita, FacundoCantu, DarioBuffalo, VinceAvni, RazVazquez Gross, HansZhao, RongrongConley, Christopher J.Distelfeld, AssafDubcovsky, JorgeWheatSenescenceZinc transportIron transporthttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4BACKGROUND: During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. RESULTS: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. CONCLUSIONS: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world.Fil: Pearce, Stephen. University of California; Estados UnidosFil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaFil: Cantu, Dario. University of California; Estados UnidosFil: Buffalo, Vince. University of California; Estados UnidosFil: Avni, Raz. Tel Aviv University; IsraelFil: Vazquez Gross, Hans. University of California; Estados UnidosFil: Zhao, Rongrong. China Agricultural University; ChinaFil: Conley, Christopher J.. University of California; Estados UnidosFil: Distelfeld, Assaf. Faculty Of Life Sciences, Department Of Molecular Biolo;Fil: Dubcovsky, Jorge. University of California; Estados Unidos. Howard Hughes Medical Institute ; Estados Unidos. Gordon & Betty Moore Foundation Investigator; Estados UnidosBioMed Central2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32851Cantu, Dario; Tabbita, Facundo; Dubcovsky, Jorge; Zhao, Rongrong; Vazquez Gross, Hans; Distelfeld, Assaf; et al.; Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence; BioMed Central; BMC Plant Biology; 14; 1; 2-2014; 368-2911471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/content/pdf/s12870-014-0368-2.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-014-0368-2info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302714/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:26Zoai:ri.conicet.gov.ar:11336/32851instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:26.871CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence |
title |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence |
spellingShingle |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence Pearce, Stephen Wheat Senescence Zinc transport Iron transport |
title_short |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence |
title_full |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence |
title_fullStr |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence |
title_full_unstemmed |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence |
title_sort |
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence |
dc.creator.none.fl_str_mv |
Pearce, Stephen Tabbita, Facundo Cantu, Dario Buffalo, Vince Avni, Raz Vazquez Gross, Hans Zhao, Rongrong Conley, Christopher J. Distelfeld, Assaf Dubcovsky, Jorge |
author |
Pearce, Stephen |
author_facet |
Pearce, Stephen Tabbita, Facundo Cantu, Dario Buffalo, Vince Avni, Raz Vazquez Gross, Hans Zhao, Rongrong Conley, Christopher J. Distelfeld, Assaf Dubcovsky, Jorge |
author_role |
author |
author2 |
Tabbita, Facundo Cantu, Dario Buffalo, Vince Avni, Raz Vazquez Gross, Hans Zhao, Rongrong Conley, Christopher J. Distelfeld, Assaf Dubcovsky, Jorge |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Wheat Senescence Zinc transport Iron transport |
topic |
Wheat Senescence Zinc transport Iron transport |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
BACKGROUND: During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. RESULTS: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. CONCLUSIONS: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world. Fil: Pearce, Stephen. University of California; Estados Unidos Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina Fil: Cantu, Dario. University of California; Estados Unidos Fil: Buffalo, Vince. University of California; Estados Unidos Fil: Avni, Raz. Tel Aviv University; Israel Fil: Vazquez Gross, Hans. University of California; Estados Unidos Fil: Zhao, Rongrong. China Agricultural University; China Fil: Conley, Christopher J.. University of California; Estados Unidos Fil: Distelfeld, Assaf. Faculty Of Life Sciences, Department Of Molecular Biolo; Fil: Dubcovsky, Jorge. University of California; Estados Unidos. Howard Hughes Medical Institute ; Estados Unidos. Gordon & Betty Moore Foundation Investigator; Estados Unidos |
description |
BACKGROUND: During wheat senescence, leaf components are degraded in a coordinated manner, releasing amino acids and micronutrients which are subsequently transported to the developing grain. We have previously shown that the simultaneous downregulation of Grain Protein Content (GPC) transcription factors, GPC1 and GPC2, greatly delays senescence and disrupts nutrient remobilization, and therefore provide a valuable entry point to identify genes involved in micronutrient transport to the wheat grain. RESULTS: We generated loss-of-function mutations for GPC1 and GPC2 in tetraploid wheat and showed in field trials that gpc1 mutants exhibit significant delays in senescence and reductions in grain Zn and Fe content, but that mutations in GPC2 had no significant effect on these traits. An RNA-seq study of these mutants at different time points showed a larger proportion of senescence-regulated genes among the GPC1 (64%) than among the GPC2 (37%) regulated genes. Combined, the two GPC genes regulate a subset (21.2%) of the senescence-regulated genes, 76.1% of which are upregulated at 12 days after anthesis, before the appearance of any visible signs of senescence. Taken together, these results demonstrate that GPC1 is a key regulator of nutrient remobilization which acts predominantly during the early stages of senescence. Genes upregulated at this stage include transporters from the ZIP and YSL gene families, which facilitate Zn and Fe export from the cytoplasm to the phloem, and genes involved in the biosynthesis of chelators that facilitate the phloem-based transport of these nutrients to the grains. CONCLUSIONS: This study provides an overview of the transport mechanisms activated in the wheat flag leaf during monocarpic senescence. It also identifies promising targets to improve nutrient remobilization to the wheat grain, which can help mitigate Zn and Fe deficiencies that afflict many regions of the developing world. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/32851 Cantu, Dario; Tabbita, Facundo; Dubcovsky, Jorge; Zhao, Rongrong; Vazquez Gross, Hans; Distelfeld, Assaf; et al.; Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence; BioMed Central; BMC Plant Biology; 14; 1; 2-2014; 368-291 1471-2229 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/32851 |
identifier_str_mv |
Cantu, Dario; Tabbita, Facundo; Dubcovsky, Jorge; Zhao, Rongrong; Vazquez Gross, Hans; Distelfeld, Assaf; et al.; Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence; BioMed Central; BMC Plant Biology; 14; 1; 2-2014; 368-291 1471-2229 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/content/pdf/s12870-014-0368-2.pdf info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-014-0368-2 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4302714/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |